FastQCFastQC Report
Sat 31 Dec 2022
SRR5848833.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848833.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148421
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT8290.558546297356843No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC7310.4925179051481933No Hit
AAGCCGGGCTTCTACCTCAGCCTGCCAAGTAGCTGTCACTACAGGTACCAGC1970.13273054352146932No Hit
TTACATGGGCTGAGAGGTGTTTAGGCACAGCAGAGGGGACGCGGGGGGCAAG1850.12464543427143059No Hit
GCACTCTAGCCTGGGCGACTGAGCCAGACTCCATCTCAAAAAATAAAGAGAA1750.11790784322973165No Hit
AGACAGCGGGGGGCCGCGCGGCGGGAGGAGGTGCCGGCCGAGCACCGCAGAC1750.11790784322973165No Hit
AGACTCGGGTGCACACTTGCTGGAACTTCTCTAGGATATATATCTAGGAGTC1700.11453904770888217No Hit
GCTTCAGGGACAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCTGT1690.11386528860471228No Hit
TTTTGAGGGCGAGGAAAAAATACGGAGGATAACCACTATTTTTGTGCAAATA1680.11319152950054238No Hit
ACTCTCGGGGATTTGTCATAGGCTTTCCCCTTTCTTTCTGTTCTAGCAGCAG1660.11184401129220259No Hit
GCACCTGGGGGCACTCAGGATTCTTTCTATCTCACTCCTCTGCTGAGATCTC1660.11184401129220259No Hit
GCCAAGGGCATTTGATGAGTCCTAACATAGAATTGATCTAGCTCTTGAAATA1660.11184401129220259No Hit
GTTTCAGGGGATTCATCTGAGGAGTCAGTCCTCTCGAAAGCTGCCAAAATAA1650.1111702521880327No Hit
TTATCTGGGATCAGTAGTGAATCTTTCACTTAACAAGATACCTTGGCCCCCA1640.11049649308386279No Hit
CTAGCTGGGGACAGTTCTTCAAGAAGCCAATTTTAGTTTTTAAAAAACAGTG1640.11049649308386279No Hit
CAAGTCGGGAGCTGGAGTCCGAGAATGATGAGTACGAGCGGGCCCGGAGGCT1630.10982273397969292No Hit
AATCACGGGCAGCTCTCGGGGCCTTTTTTCCAGTGCCCATTTGGCTACTCTG1630.10982273397969292No Hit
CTGCCAGGGCATTCTGTCCAGTTTCCTAAGAGAGACAGCTTGGAGTATGCTT1620.10914897487552301No Hit
AAAGTCGGGTACAAGGCCTCAGAATTATTTGCCCAAGTGTATTCTTTAAATA1610.1084752157713531No Hit
AGCAGCGGGAAGGTGGGCGAGGCCTAGGAAGAGGGTCATTCTTAAGCCACAC1600.10780145666718323No Hit
AGTTATGGGATGCCTGGCACAGCCAGGAAGGAGCTGCCCATGGGATTGTCAT1580.10645393845884342No Hit
GAACGAGGGGAGGGTCTTGTCTGTTAGAAGGAAAACTAACAAACAGAAAGGA1580.10645393845884342No Hit
TCCTGTGGGATGAAGGTAAACTTGACGGACTAATAAAGGAAACTGAAGAGAA1560.10510642025050364No Hit
ATAGCAGGGGCTTTTAGTGCTACTGGTGCTCTTGAAGGACAGATGTTCCGGA1550.10443266114633373No Hit
CACACTGGGATTCTTTGTAATGATGGTCTTTGTCTTGGACCCTATTTTGGAT1550.10443266114633373No Hit
TGGGTTGGGCTTCTCGTTGGTCAGACCGATGTCCATTACATTCTCACACACA1550.10443266114633373No Hit
GGGTATGGGATCTGACAATGGGTGGCTCCCTGGGCCTGTTCATCCATCCCGG1540.10375890204216383No Hit
TCTTAAGGGTACAGAAGAATTGGAATGAAGACAGGATTTTTTTTGTCAAAGA1540.10375890204216383No Hit
CCACGTGGGCAAAATCACTGAAGAAAATCGCTGGCATCAGTGTGCAGCAAAT1530.10308514293799395No Hit
CAAACAGGGGAGGTAAGATTGTTCTGTCAGAAAACCCTCTCCCAGTTCCCCT1510.10173762472965416No Hit
AAACTAGGGAGGAGGAGTGACGGGAACCGGGAGCCGGCTCTATGCGCCTGGA1510.10173762472965416No Hit
AAATAAGGGGTTGTTTAATATGCATGACATTTTTATGATAGAAAAGATTTTT1510.10173762472965416No Hit
GCCATAGGGAACAACCTTCATGGAATTTCTAGTGGAATTGAATTTATAGAAA1490.10039010652131436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)