FastQCFastQC Report
Sat 31 Dec 2022
SRR5848832.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848832.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146234
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC7250.49578073498639164No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT7080.4841555315453315No Hit
AAGCCGGGCTTCTACCTCAGCCTGCCAAGTAGCTGTCACTACAGGTACCAGC1820.12445806036899763No Hit
TTACATGGGCTGAGAGGTGTTTAGGCACAGCAGAGGGGACGCGGGGGGCAAG1700.1162520344106022No Hit
TTTTGAGGGCGAGGAAAAAATACGGAGGATAACCACTATTTTTGTGCAAATA1680.11488436341753627No Hit
CAAGTCGGGAGCTGGAGTCCGAGAATGATGAGTACGAGCGGGCCCGGAGGCT1660.11351669242447038No Hit
ATTCCAGGGGCATTAAGAGATATAAATGCAATAGTATTTAAAGTGTCAGGCA1640.11214902143140446No Hit
TCCTGTGGGATGAAGGTAAACTTGACGGACTAATAAAGGAAACTGAAGAGAA1620.11078135043833856No Hit
GCCAAGGGCATTTGATGAGTCCTAACATAGAATTGATCTAGCTCTTGAAATA1610.1100975149418056No Hit
CTTTAATCGGGCAGTGTGAGAGGAATGATGGTATTCTATTTTATATAATTGT1600.10941367944527264No Hit
TAGCCAGGGATTGGTCATTGATATCAGTATTATTATTTTGCATCCTCTGCTA1580.10804600845220674No Hit
TTAAAGCGGGAAGGTCTGATGACCCTGTCGAAGATGATAAAGAAAAAAAAGA1570.10736217295567378No Hit
ACACGGGAATGACAATGGATAAAAGTGAGCTGGTACAGAAAGCCAAACTCGC1540.10531066646607493No Hit
ACTCTCGGGGATTTGTCATAGGCTTTCCCCTTTCTTTCTGTTCTAGCAGCAG1540.10531066646607493No Hit
AGACAGCGGGGGGCCGCGCGGCGGGAGGAGGTGCCGGCCGAGCACCGCAGAC1540.10531066646607493No Hit
CAGTAGCGGGGTGAAAAGTGAGAACCAACTTTTAGATAGAAAGTTATTTGAG1540.10531066646607493No Hit
ACACTAGGGATTCTTTCCTGTCACCATTATATGTCTTTATGAAGCAAGGGAG1530.10462683096954196No Hit
TAGCAAGGGGTGGCTGTGGCATCTCAGTCCTATTTTTGAGTTTTTTTGTGGG1530.10462683096954196No Hit
TATCGAGGGATCCTTGCCAGCATTTGTTGTTGCCTGTTTTTAGGTATAAAAG1520.103942995473009No Hit
TTCAAGGGTTTCTTAGTTGGGTGAATTAAAGGTTAATCCGAGAATTAGCATG1510.10325915997647607No Hit
AGCAGCGGGAAGGTGGGCGAGGCCTAGGAAGAGGGTCATTCTTAAGCCACAC1510.10325915997647607No Hit
TCTTAAGGGTACAGAAGAATTGGAATGAAGACAGGATTTTTTTTGTCAAAGA1510.10325915997647607No Hit
TGCGGATGGGACAAATATTGTGATTTCCCAGGGGTTACAAGAGAGGAGACAC1500.10257532447994311No Hit
TGGGTTGGGCTTCTCGTTGGTCAGACCGATGTCCATTACATTCTCACACACA1500.10257532447994311No Hit
AAGCCAGGGAAATGTTTAAGAAATGAATAAATAGAAGTTTATTCCCTGCAGG1480.10120765348687719No Hit
GAACGAGGGGAGGGTCTTGTCTGTTAGAAGGAAAACTAACAAACAGAAAGGA1470.10052381799034425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)