FastQCFastQC Report
Sat 31 Dec 2022
SRR5848821.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848821.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138631
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT4930.3556203158023819No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC4360.31450397097330324No Hit
AGATTCGGGGTATGTTGTGAGCTGTGGGCCTTTATCAGGTTAAGGAAGTTTT2020.14571055535919095No Hit
TCTTTACGGGGGGAGACGAAGATGGCGGCGGCGGCGGCGGTGACGGCGCTTC1970.14210385844435947No Hit
AAATGTGGGGTAATTTCTTTTTTGGTTTGTTTTTTTAAAAGCAAACGAAACG1960.1413825190613932No Hit
AGCCATGGGAGCTTGAGTGAAAGGAGGGTGAGGCTGCCGATGCCTTCCTGCC1940.1399398402954606No Hit
CATCATGGGATAGGAAGTAACCGGTTTTGTGAGGATTGGATGCAAGCTTTTT1930.13921850091249433No Hit
CCTCGGGGGTAAAACCAGATGATCAGCAAAAGGAAGTGGTTAACATGGCCCA1890.13633314338062913No Hit
CGTCTCGGGCAGACAAACGCTGTGTTCTCACATGGCAGAAGAGTGGGGAGAG1860.13416912523173027No Hit
GCAATGGGGAACAAAGGCTGTGAGTTCTCCTCTTTCCTTCCACAGGACAAGG1810.13056242831689882No Hit
TAAGGCGGGAGCGCGAGCAGCGGGAGCGGGAGCAACGCCTCGAGGCCTTCCA1810.13056242831689882No Hit
CTGCGGGTCTACAAATCCATTTAAATGGTATTGGAGGGTGAGTAAAACCTTG1810.13056242831689882No Hit
ACTCGGGGGACTTTCTGTGTGAGTATGTTACAGTATTTCTTTCCATTCTGCA1800.12984108893393254No Hit
TGCAGTGGGGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACGT1790.12911974955096625No Hit
ACATTCGGGTTCCTTTAACCGGATTGTGAAAGCTTCATGTATTTTAATTTAG1790.12911974955096625No Hit
CATCTGGGGATGGATTGCTGGTCTTCCGAGCTCTATAATGTAGTGAAATAAA1770.12767707078503365No Hit
TACGCTGGGATCTGAGTTGCGGGGAGGAAATTAAACAGAAACATGATTAGCT1760.12695573140206737No Hit
GTACAAGGGTTAAATTTAGCTCTTTAAAATCCTTGTCCTATGTCTGACTTGT1750.12623439201910105No Hit
CGAACGGGGTCCTATGTGGTCTTTTATCTTCCATGTAATGGTTTTCAGGCAT1750.12623439201910105No Hit
TAGGCAGGGGAATCGACCTCCATCCTGAGCTCTCCAGGCCTGGGACAATGGA1740.12551305263613477No Hit
TGCTTCGGGGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATATATTTT1730.1247917132531685No Hit
ACATCCGGGATACACCAGTCTAAGCACCTGGGCTAAGACTGGAGCCCATGAT1720.1240703738702022No Hit
AGCAGCGGGGCGGCTTAGCTTGACTGTGACTATTTAAACCTTGGCCCTCCAC1700.1226276951042696No Hit
TGGTTCGGGGCAGCCAGACCTGGTGGTCCTTTCTGGATTGCACATGATGGAG1690.12190635572130332No Hit
AATATGGGGCTTTTAAAAATCAAAGTCCAACAGCAGTGTGTTACTTACTGTC1680.12118501633833702No Hit
CTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAA1660.11974233757240443No Hit
ATAGTGGGGGACACATGAAAGGAGGTATAGGAACTTTGGCATTCGTTTCTGC1660.11974233757240443No Hit
TACAGCAGGGGTAGAATAAGAATCATGGATGAGAAAATACTCTGAAACAATG1640.11829965880647186No Hit
TCGACGGGTATATCACAAGAAAATAAAACTGTAGGTGAAAATTCATGATGAA1630.11757831942350556No Hit
CTCTGACGGGATGTGCAGTACTTAATGTTTGTAAGACTTACAAAAAAAGATT1630.11757831942350556No Hit
TTTATGGGGGTGAAGCTTGGAACAATAAAACCAAAGGAAGAATACATCAGAG1630.11757831942350556No Hit
GTGCTAGGGATAGGAGTTCCCATTTGGTTGAAAGCTAGAAGCCCTAATTGAC1620.11685698004053928No Hit
CGCAATGGGTTTGTAGGAATTTATTTATGAATGTCTATGGCCTGCCAGTGTA1620.11685698004053928No Hit
ATCATGGGGAAGGACAAAGGTGGCCCAGAATTTTCCAGTGCCCTGAAAAATA1620.11685698004053928No Hit
GTGGTCGGGGAGCGCTGGAAACTGGGTGGACGGCATGGGTTGGTCTCAGGAT1600.11541430127460668No Hit
CGAATCGGGCTGGCCTACTGGGCTGACATTAACTACAATTATGGGAAATGCA1600.11541430127460668No Hit
AAAAGCGGGATTGCGCAATTATAGATCTGGATTTTGAACCACTTAATGAAGC1590.11469296189164038No Hit
TTGAGGAGGGGAGCCGCCGGACGGACGCTCGTCTTCGCCCGCCATGGCCGAG1590.11469296189164038No Hit
CTTTCAGGGGTATCCAGGAGAGGAACTGCATAACCAAAGGGGTGACCAGCCC1580.11397162250867411No Hit
GCTACTGGGCTCGGAGCATGGCAGCTAAGTTAGTGAGGGAAGGTGAGATGGA1570.11325028312570783No Hit
GTTCTTGGGGAAGCAGAATGAGGGAAGAAATTTTGCCTCCCTCAGACAAGCA1570.11325028312570783No Hit
GTCTTTGGGCAATCTTACAACTCATAGCTTCCTGACTATTTTCAGGTATTTT1550.11180760435977523No Hit
AACTACGGGACATGCAAATAACAGACTTTCCATAATCTACTGTGACAAGGTG1540.11108626497680894No Hit
GAATACGGGCTTCCCCCTCTTTCTTTAAAGAACTGCTCAATATTCAATCTGT1540.11108626497680894No Hit
AAAAATGGGGAAGTTGGTATATGCATGAGCAGATACATATTTATTTAAGAAA1540.11108626497680894No Hit
AGCATAGGGGACGTGGAAGGATTTGAGTGTGGTTTCTATGTGGCAAGATCAA1530.11036492559384264No Hit
ACTAGGGGATGTCTCACATTTTAAATCAAAAGCTAGAAATGAAGCTCAATGA1530.11036492559384264No Hit
CATGTCGGGTCAGCCTGGTTTGAATTTAATCATCTCTTTAGATCTTATAAGG1520.10964358621087635No Hit
TTAATAGGGAACCATTAAGCTGTAGTGCCTCCTTTACCAATGCTGACCATTA1520.10964358621087635No Hit
AGGCATGGGGAATCTCTTTGGTGTAGACCTATAAATAGATTCTCGTCTTTTC1510.10892224682791006No Hit
GCGGTAGGGGAAAACCAATCAGGTGAATTCACCATAGTAACAAACTGAAAAA1510.10892224682791006No Hit
AAATAAGGGGATCTGCCATGGGACATAAGATGAACGATCACACACAGCACGT1500.10820090744494378No Hit
GTCGGCGGGAGTATTGGGCAGCATTCTTGGAGCACTGAAGGCCATTGTAAAT1500.10820090744494378No Hit
ATGACAGGGGATAGTCTTGGATTGCATTAAATGCAGCACATTTAAAAATATG1500.10820090744494378No Hit
ACACCCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1500.10820090744494378No Hit
GTTACAGGGTAATTGAGAGGTGAGAACATAAGATTTCCTCTGGAAATTTGCT1490.10747956806197749No Hit
GTATTGAGGGAATTAGTTTCTCAGGAACACAAACCCTATTGTGAACTGCGTA1490.10747956806197749No Hit
CTGTTAGGGAAATGGAAGACACATTCATCCTTCTCCCTCTGACTGCTTTGAT1490.10747956806197749No Hit
CTTTTGGGGGGAGCAACCCGCGAGCTTACACCGGCTTCTCTCTGTCCTCAGC1480.10675822867901119No Hit
TTGGAAGGGCGCTATGCAGTCAGGGATGAGAAGGGGATTGTGATTGAGTAAA1480.10675822867901119No Hit
CGAAATGGGAAGACAATTTACAGTCTTTGACTAAGCATCCACACTGGAAGAA1480.10675822867901119No Hit
CCTACAGGGGCGAGCCTGCGTTTTCCGGCCAGAGGACATGATGCAGGGGGAG1480.10675822867901119No Hit
TTAGTCGGGGAAAGAAAATGGTTTTCTACCATTTCGACTGGATCATATCAGA1480.10675822867901119No Hit
ATTCCTGGGGCATATAATTTTATATTCTGCTTTTGTTATCATTTTCCTTGTT1470.10603688929604489No Hit
TATTAGGGGATCTTTTGGAGGGACACGTACCCAGCAATATCAGTTGTAAGCA1470.10603688929604489No Hit
AATGCCGGGCTACCAACGGGAGTTTTGAGTCGGTATGACACCACTGCAGTGT1470.10603688929604489No Hit
TGACGTGGGGTTTGCAAAATACCTGAGCAGGATTCCTTAAAACAGTCAAGGT1470.10603688929604489No Hit
TCGAGGGGCTTCGGGAGTATTAAGACATAAGCCTAGAAAGGTAGGCAAAGGT1470.10603688929604489No Hit
GCTCATGGGGGGAACACCTTAGAGAAAAAAGACTTTTAGGTAAGATTGGTTT1460.10531554991307861No Hit
GCCACTGGGTGTAGCAGGCATACATGCAGGATGTATCCCATCTCCCCTGATT1460.10531554991307861No Hit
AATAACGGGCTACAGAAAATAAACAGGGTGATCATAATTACCACATCAAATT1460.10531554991307861No Hit
GTTACTGGGACTGAGGATTACAAAATGATTATATTCTGTCTAATTCTATCAG1450.10459421053011231No Hit
TGCTACGGGGATGTCCAATGAGGTAGTAGTATATACATCTAAGGCAATAACA1450.10459421053011231No Hit
AGCTTGGGGGCGGCGCCGCCATGGCCGACGTGGAAGACGGAGAGGAAACCTG1450.10459421053011231No Hit
TCTTCTGGGGGCTCCGCGCACCTGGGCGCCATCCGCCCTGGCTCCGCTGCAC1450.10459421053011231No Hit
AAGCACGGAGCCTTATTTCAGGTATATAAAAATGTGGAAAAAGTTGCCTTTT1440.10387287114714601No Hit
AAGCAGGGGAACCTGATCTGTCCAGTCTAGAAGGATTCCAGTGGGAAGGTGT1440.10387287114714601No Hit
TATGACGGGTCTTTGGGGCTTCTGTGACTTGGGAAATGCTTACATTCAGGCC1440.10387287114714601No Hit
CCAGCTTGGGACACTTCGGGAATTTATAAAAATCTACAGATGAATCAGTCCT1440.10387287114714601No Hit
ATCGCAGGGGTGATATCCTGTGGTCAGACCCCCTGGAAGATTTTGGAAATGA1440.10387287114714601No Hit
GTCTAAGGGACCCTATCAACTGGAGAGCCCACAAGGCATAGGGAATAGAGAA1430.10315153176417972No Hit
TAACAAGGGCTGGAGAATGCATCCATCCTTTCAGAGGGCTCTCTGCAGGAAG1420.10243019238121345No Hit
TGGCTTGGGTCTTGTCGCGGGTGCTTCCCCTAAACTACTCCTGAGCATCCCA1420.10243019238121345No Hit
TTAAACGGGATTTGTTACAAGTTAAAGTCCAGAAGTATACACAAGATTGATT1420.10243019238121345No Hit
ATATATGGGAGACAACCATCTGTGAGGTCAGTCATTTTGCATGATGTATGTA1410.10170885299824715No Hit
TGCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1410.10170885299824715No Hit
CTATCAGGGGGCATAACTTTGTCCCAAGAATTATTTGTTGTAAAAGTGAGAA1410.10170885299824715No Hit
CCATTTGGGGGTGGCGGCTTCCTGTGGTGTTTGTGGACGTCCTCTGCCTGTG1400.10098751361528086No Hit
TTCTCAGGGATACATTTCATGTGTGTCCTGTTCATACTCGCACTAGTGTTTT1400.10098751361528086No Hit
GCACGCGGGGAAGAAGAGTTTAGAAGTCTCTTTGAATAAAGGTTTTGTTCAT1400.10098751361528086No Hit
GGTGCTGGGATTACAGGTGTGAGCTACTGTTCCCCGCCTAAATCTTTTAGTT1400.10098751361528086No Hit
ACGCATGGGCGGGACGGGGTCATTGAGATGGGTAGACACCTGCAAGAGGAGT1390.10026617423231456No Hit
GTTTTAGGGCACAAAAAAGAAAAAATAAATTGGACTTCATCAACATCTAAAA1390.10026617423231456No Hit
CAAAGAGGGAGCAAAATCAAAGTGATTTGGACAAATATGGGAAACACAGTCT1390.10026617423231456No Hit
ATATACGGGAGTCCAACTGTTACAACCACTCTAAAAAACCTTGGGGCACTTT1390.10026617423231456No Hit
GCAGGGCGGTATCCAAGAAACATACATACATTCTAAATGGTATATATTGGGA1390.10026617423231456No Hit
TTACTACCGGGCGATCTGTTTATGGGCGATCCACCACCGGGGAAATGGGGTA1390.10026617423231456No Hit

[OK]Adapter Content

Adapter graph