FastQCFastQC Report
Sat 31 Dec 2022
SRR5848820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136932
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT5040.36806590132328454No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC4260.31110332135658575No Hit
AAATGTGGGGTAATTTCTTTTTTGGTTTGTTTTTTTAAAAGCAAACGAAACG1900.13875500248298425No Hit
CATCATGGGATAGGAAGTAACCGGTTTTGTGAGGATTGGATGCAAGCTTTTT1870.13656413402272663No Hit
GCTACTGGGCTCGGAGCATGGCAGCTAAGTTAGTGAGGGAAGGTGAGATGGA1770.1292612391552011No Hit
CATCTGGGGATGGATTGCTGGTCTTCCGAGCTCTATAATGTAGTGAAATAAA1740.12707037069494348No Hit
AGATTCGGGGTATGTTGTGAGCTGTGGGCCTTTATCAGGTTAAGGAAGTTTT1740.12707037069494348No Hit
TGGTTCGGGGCAGCCAGACCTGGTGGTCCTTTCTGGATTGCACATGATGGAG1740.12707037069494348No Hit
TGCTTCGGGGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATATATTTT1720.12560979172143838No Hit
TTAAAAGGGAGATTGAGACAATTTGAAAAAACTCAAAACTGTGTAGTGTAGA1720.12560979172143838No Hit
TCTTTACGGGGGGAGACGAAGATGGCGGCGGCGGCGGCGGTGACGGCGCTTC1710.12487950223468583No Hit
CGTCTCGGGCAGACAAACGCTGTGTTCTCACATGGCAGAAGAGTGGGGAGAG1680.1226886337744282No Hit
ATAGTAGGGATTTCAAGTTCTAATTTACACTTATTTCAGTGTGGTTTTCATA1660.1212280548009231No Hit
GTTACAGGGTAATTGAGAGGTGAGAACATAAGATTTCCTCTGGAAATTTGCT1660.1212280548009231No Hit
AATATGGGGCTTTTAAAAATCAAAGTCCAACAGCAGTGTGTTACTTACTGTC1660.1212280548009231No Hit
TACGCTGGGATCTGAGTTGCGGGGAGGAAATTAAACAGAAACATGATTAGCT1650.12049776531417053No Hit
CCTCGGGGGTAAAACCAGATGATCAGCAAAAGGAAGTGGTTAACATGGCCCA1650.12049776531417053No Hit
CGAAATGGGAAGACAATTTACAGTCTTTGACTAAGCATCCACACTGGAAGAA1630.11903718634066543No Hit
CTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAA1630.11903718634066543No Hit
AAAAGCGGGATTGCGCAATTATAGATCTGGATTTTGAACCACTTAATGAAGC1590.11611602839365524No Hit
TACACTGGGAAGTACCAATGACTATACACTATTCTCATTACATTACTTTGTA1590.11611602839365524No Hit
ACATTCGGGTTCCTTTAACCGGATTGTGAAAGCTTCATGTATTTTAATTTAG1580.1153857389069027No Hit
CTGCGGGTCTACAAATCCATTTAAATGGTATTGGAGGGTGAGTAAAACCTTG1550.11319487044664504No Hit
ATTCCTGGGGCATATAATTTTATATTCTGCTTTTGTTATCATTTTCCTTGTT1540.11246458095989251No Hit
TGCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1540.11246458095989251No Hit
CTTTCAGGGGTATCCAGGAGAGGAACTGCATAACCAAAGGGGTGACCAGCCC1530.11173429147313994No Hit
CGTTCCGGGCTATCTGAGACCTTGCTTTCAATTCTTTCGAGTGTGTACACAG1530.11173429147313994No Hit
CTTTTGGGGGGAGCAACCCGCGAGCTTACACCGGCTTCTCTCTGTCCTCAGC1520.11100400198638741No Hit
CTGCTGGGAGTGCCAGAATTAGTAGTCTTATTCCAAAAATTCTAATTCATAT1520.11100400198638741No Hit
TTTGCAGGGTATTAATGTTGGGTAAAATGGGGAACCACCATTGGATATCTAT1510.11027371249963484No Hit
GCGGTAGGGGAAAACCAATCAGGTGAATTCACCATAGTAACAAACTGAAAAA1500.10954342301288231No Hit
TGCAGTGGGGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACGT1490.10881313352612977No Hit
CGAATCGGGCTGGCCTACTGGGCTGACATTAACTACAATTATGGGAAATGCA1490.10881313352612977No Hit
GTACAAGGGTTAAATTTAGCTCTTTAAAATCCTTGTCCTATGTCTGACTTGT1480.10808284403937721No Hit
AGCCATGGGAGCTTGAGTGAAAGGAGGGTGAGGCTGCCGATGCCTTCCTGCC1480.10808284403937721No Hit
AATGCCGGGCTACCAACGGGAGTTTTGAGTCGGTATGACACCACTGCAGTGT1480.10808284403937721No Hit
GTGGCTGGGGGCTGAGATGTACGAACTTCCGGTTCTCCGGGCAGCTGCCACT1480.10808284403937721No Hit
AGCATAGGGGACGTGGAAGGATTTGAGTGTGGTTTCTATGTGGCAAGATCAA1460.10662226506587211No Hit
AACTACGGGACATGCAAATAACAGACTTTCCATAATCTACTGTGACAAGGTG1460.10662226506587211No Hit
GTAGTTGGGAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAA1460.10662226506587211No Hit
CTATCAGGGGGCATAACTTTGTCCCAAGAATTATTTGTTGTAAAAGTGAGAA1460.10662226506587211No Hit
CGCCTGGGATGCTTGAATTTCCTCAGTGGGTTGTATGTTTGTCCATACATAC1460.10662226506587211No Hit
TAGGCAGGGGAATCGACCTCCATCCTGAGCTCTCCAGGCCTGGGACAATGGA1460.10662226506587211No Hit
AAGATTGGGACAGGTCCTCTGCCTCATATGTTTTCTATACACACAGCACTTT1450.10589197557911957No Hit
ATATACGGGAGTCCAACTGTTACAACCACTCTAAAAAACCTTGGGGCACTTT1440.10516168609236701No Hit
CTACATGGGGGATAAATAATCATGGAAAATAAATTCCAGACGAGGTCAGATA1440.10516168609236701No Hit
GGTGCTGGGATTACAGGTGTGAGCTACTGTTCCCCGCCTAAATCTTTTAGTT1440.10516168609236701No Hit
CATGTCGGGTCAGCCTGGTTTGAATTTAATCATCTCTTTAGATCTTATAAGG1430.10443139660561446No Hit
AGCAGCGGGGCGGCTTAGCTTGACTGTGACTATTTAAACCTTGGCCCTCCAC1430.10443139660561446No Hit
AGGCATGGGGAATCTCTTTGGTGTAGACCTATAAATAGATTCTCGTCTTTTC1430.10443139660561446No Hit
GCCACAGGTTATTTTTATCTTTAATAGTTATATATGAGAGGAAGGGTCAACA1420.10370110711886192No Hit
CCTACAGGGGCGAGCCTGCGTTTTCCGGCCAGAGGACATGATGCAGGGGGAG1420.10370110711886192No Hit
TGACGGGGGACGGCCCACGGTGGGCACCAATCTTACTGACATCGTGGCACAG1420.10370110711886192No Hit
TTAAACGGGATTTGTTACAAGTTAAAGTCCAGAAGTATACACAAGATTGATT1420.10370110711886192No Hit
TCGAGGGGCTTCGGGAGTATTAAGACATAAGCCTAGAAAGGTAGGCAAAGGT1420.10370110711886192No Hit
GTAACTGGGGTTCCTCGCCCTGTGACCATGTGTTCTCATTGTTCAGCTCCCA1410.10297081763210936No Hit
GTCTTTGGGCAATCTTACAACTCATAGCTTCCTGACTATTTTCAGGTATTTT1410.10297081763210936No Hit
AAAAATGGGGAAGTTGGTATATGCATGAGCAGATACATATTTATTTAAGAAA1410.10297081763210936No Hit
AAATAAGGGGATCTGCCATGGGACATAAGATGAACGATCACACACAGCACGT1400.10224052814535682No Hit
TATGACGGGTCTTTGGGGCTTCTGTGACTTGGGAAATGCTTACATTCAGGCC1400.10224052814535682No Hit
CAAAGAGGGAGCAAAATCAAAGTGATTTGGACAAATATGGGAAACACAGTCT1400.10224052814535682No Hit
GGCAACGGGCATTAATCCTGGAATAAAAGAAGCCGAAATAAATGAGAGATGA1400.10224052814535682No Hit
AATAACGGGCTACAGAAAATAAACAGGGTGATCATAATTACCACATCAAATT1400.10224052814535682No Hit
GTTTTGGGCAGCTGGGCTCCAGTAGGCTTTAAATGTTCATTTGTTTTCCAAT1400.10224052814535682No Hit
GTGCTAGGGATAGGAGTTCCCATTTGGTTGAAAGCTAGAAGCCCTAATTGAC1390.10151023865860426No Hit
CGAACGGGGTCCTATGTGGTCTTTTATCTTCCATGTAATGGTTTTCAGGCAT1390.10151023865860426No Hit
ATATTTGGGTATATACAAATGAATGTTATGAGGACATTTTATGAGTTTCAGT1390.10151023865860426No Hit
GGCTTTGGGGAAAGGGGGAGGAGGGAAGAAGGAAGGATGGGAGAGAAAGACA1390.10151023865860426No Hit
GTTGCAGGGTATGTGGTATATGGCAAATGACATATAAATGTTTATAATATTA1390.10151023865860426No Hit
ACTTACGGGGAAAGGAGATTTGATATGTGTATCTTATGGCTCAGTATGCCCT1390.10151023865860426No Hit
ACATCCGGGATACACCAGTCTAAGCACCTGGGCTAAGACTGGAGCCCATGAT1380.10077994917185172No Hit
TTCTCAGGGATACATTTCATGTGTGTCCTGTTCATACTCGCACTAGTGTTTT1380.10077994917185172No Hit
CATACTGGGAATAAAAGAAAGTGAATAGATGTTATTTCCTACTTTAGAAAGT1380.10077994917185172No Hit
CGTGGCGGGCAACTACTGCCACATCCTTATACTTTATTGCTTTTCTGTCTTC1380.10077994917185172No Hit
TTGAGGAGGGGAGCCGCCGGACGGACGCTCGTCTTCGCCCGCCATGGCCGAG1370.10004965968509918No Hit
TCGACGGGTATATCACAAGAAAATAAAACTGTAGGTGAAAATTCATGATGAA1370.10004965968509918No Hit
CTTCAAGCGGGGCTCATTGTAGATTCTGGATATTAGCTCTTTGTCAGATGAG1370.10004965968509918No Hit
GAATACGGGCTTCCCCCTCTTTCTTTAAAGAACTGCTCAATATTCAATCTGT1370.10004965968509918No Hit
GTTGCTGGGTCTTTCCACTAATTTCAAAGTCTATTTTTCTTATTACTAAAAT1370.10004965968509918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)