FastQCFastQC Report
Sat 31 Dec 2022
SRR5848781.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848781.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102905
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC9120.8862543122297265No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT8180.7949079247849958No Hit
ACACGTGGGGAGATTGCCGGTCTCTCCTATTCAGGTGACTTTCGGATGGTGG1640.15937029298867889No Hit
CTAACAGGGGAAAGCCGGAAGGGGCAAGACGGGTTCAGTTCGTCATGGGGCT1590.15451144259268257No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGA1570.15256790243428406No Hit
TTGCTTGGGAACAGGGACTTGGTTCAATAAATTATGGTATATCCATTAGTGG1480.1438219717214907No Hit
CGTACAGGGGAGATTCTGAATTGTTAAGTATAGAAATCAATTTGATTTTTGT1310.12730188037510323No Hit
GCTAGGGGGCAATTACTGAGAGACAACTTGACATTGTGGACATCGGATACCC1290.12535834021670472No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCCTGTCTCTTATAC1280.12438657013750547No Hit
CCATTAGGGCCAGTGGTTACCTATCACAATTAACAAAGAAATCCAAGCTCTT1260.12244302997910694No Hit
CGATTAGGGATTTAAGTAGATTATACGACATCATTTTTAAAAAAGCAGTTAG1240.12049948982070843No Hit
ACTCCTGGGACTACTGATTTTATTAAATCAGAGTCTTTAATTCTTGCATGTT1210.11758417958311063No Hit
GTAGTAGGGCAGGCATGAGCCATCACATCTGGCCCAAATAACAATTTTTGAC1190.1156406394247121No Hit
AAATATTGGGGCTCTTCCGATCTAACAACGGGAAAAAAAGAGCAAAGGAAAC1170.11369709926631359No Hit
TAGTCAGGGGGTATTTCTATTCTTTAAATAAAATGCAAGTTGACATCTTGAA1160.11272532918711432No Hit
TCCAGCAGGGCCTTACAGATATTTTTCTCCAGAAGATGGTGCTGGGTAATAA1150.11175355910791507No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAAACTCGTATGC1130.10981001894951656No Hit
ATAGCAGGGTGTGACATGGTGGTTTGAATTCTTTGCCCGATTATCAATTTAG1120.10883824887031729No Hit
ACTGGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1100.10689470871191875No Hit
AAATGTGGGTGGAAAACACATGGATGTTGTTTAGTGAAGTTGAACATGTACG1100.10689470871191875No Hit
CATATGGGGAAAAATCCAATATTTGTAAGCATTCACTGGATGTGTTCAGCAT1090.1059229386327195No Hit
GCTCGGGGGAAAAGCCAGGGATGCTCTCGGTGAGTTATCACAGAAATGATGT1090.1059229386327195No Hit
ATCCCAAGGACAGAGGGGAAGGAATCTCAACACCAGAAGGAATGAAAATGTA1090.1059229386327195No Hit
ACCGCAGGGAGGGTGATAAATAGCCACTGAATGGAGAGGAAAGAGTGAAGGA1080.10495116855352023No Hit
GGTACGGGGAACTGCAACGCAGAGAAACTATTAAAGGTATTCAGAAACGTGA1070.10397939847432097No Hit
TGCTTTGGGAAGTGGGTGCTGGGCTGGTATGGGAGCCCCGGGTCCCTGGAGC1070.10397939847432097No Hit
CAAACCGGGATCCCTTGTCTATTCCTGTCTGTATAAAGTGTTTCATTATGAC1060.10300762839512172No Hit
CTGCCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1050.10203585831592245No Hit
TTGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1040.10106408823672319No Hit
AATAGGGTGAAGACATGTCTAATTTTGATCGTTTCTCTGAGATGATGAACAA1040.10106408823672319No Hit
ACGTCCGGGTAATAGTAATAAAAATAACGTTTTATGACTATTTATTGCAAGG1030.10009231815752394No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)