FastQCFastQC Report
Sat 31 Dec 2022
SRR5848774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115147
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC11931.03606694051951No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT11731.0186978384152432No Hit
GATGCCGGGAGTACATAATATGTATTTCCATTTGTCCAGATCTACTTTTGTG1700.1476373678862671No Hit
GGTTCCGGATTATCTGTTAGTTTGAAAGCAGACAAGTAAATCCCACCTTTGA1670.1450320025706271No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCCTGTCTCTTATAC1600.13895281683413374No Hit
CGCAAAGGGAGGTTATGATGTTATCATCAGTCCCAGTGCTATCAGTCAACTC1550.13461054130806707No Hit
AAACAAGGGATGATTATTTGAAATACACTTGTGTTAGTAACTGCTTCTATAA1510.13113672088721373No Hit
ACCATAGGGCTTCATAACACTGTACCAATTCAGCTTTTAAATTTTATTACTT1510.13113672088721373No Hit
TACGCTAGGGATCAGCCTCCACCTGGAAGAGAGCTAGGGCCGGGCAGGCCGG1480.12853135557157372No Hit
GCATAGGGGTCTTGCGAAGGGGTATCACATCTAGAGAAAATAATTTTGGGTG1460.12679444536114703No Hit
TATGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1440.12505753515072038No Hit
AATTGCGGGAGAAATAAATGTGTGTTTATTTCATTATTTTTGGGATGTCCTC1260.10942534325688033No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAAACTCGTATGC1260.10942534325688033No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGA1210.10508306773081366No Hit
GTTTGGGGGTCTGCAAACGGGGCATGATTCAGAGGAATAGAAAAAAGAATGA1200.10421461262560032No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCCTGTCTCT1200.10421461262560032No Hit
AATTGTGGGAATGCTGGGAGTGTCACCTGGTGCAGTTGTTTTGGAAAACAGT1190.10334615752038698No Hit
GCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA1180.10247770241517365No Hit
GCACTGGGGCTTTGCACACATTTTAAAAATGTGATTATCTTCATTTTGCAGA1180.10247770241517365No Hit
AACCATGGGCATAAGCAAAGGAGAAATGAAATTCTTTTCAGGAAAGCCAAAG1170.10160924730996031No Hit
CTATATGGGGAGTTGCTTCAGCGTCCCGGTGTGGCTGTGCCGTTGGTCCTGT1170.10160924730996031No Hit
TCCAATGGGTATGGTCACCCCCACTATCCCCATGACCGCATGAAGAGGCAGT1160.10074079220474697No Hit
TACACTGGGTATAGTAATTTTAAAACAGCTTAGAGTTGGCAAAAACAAAACA1160.10074079220474697No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)