FastQCFastQC Report
Sat 31 Dec 2022
SRR5848758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139466
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC7570.5427846213414022No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT6990.501197424461876No Hit
CGACCTGGGAAACCATAACAATTATAAAGATAACATCAGAAAAAATCCTTCT2280.16348070497468917No Hit
TTAGCTGGGGCAAAGGGAATGCCCGGACACAATGGAGAGGCTGGCCCAAGAG1880.13479987954053319No Hit
ATTGTGGGGACCAAGCAGAGACCTCTGCCTCACACACAATCAGACTGATGAT1860.1333658382688254No Hit
AATCTTCATACCCCAGTCAAGTTTCTCCATTGCTCAATAATGTATAATTTAT1770.1269126525461403No Hit
GAAAAGGGTAAGACAACAAAAGAAGTTTGCTGCATCAATTGACTCTTCCTTT1730.12404457000272469No Hit
GCACTCGGGAACCTAAGTGTCAATCAGTGGGTTAATGGATAAAGAAAATTGT1670.11974244618760128No Hit
GGTGGTGGGATGACCTGCTTCTGTGTTTATGATCTTAAGTGATAAAGTCTTA1650.11830840491589348No Hit
CTTCAGCCTGGGAGGTTGAGGCGGCAGTGAGGCAGGATCGCACCACTGCATT1650.11830840491589348No Hit
ACAGTGGGGGATCTAAACATGGGTGGTGGAAGAACTGGCCTGTGATGGCCAT1640.11759138428003958No Hit
ATCTACGGGGTGAGAGCCATTATAAGTGAAATCTAAAAATTGATCGTTTTCA1630.11687436364418567No Hit
TTTCATGGGAGAGCACTCTATCTAAATAACAGCAGAATACACGTTCTTCTCA1630.11687436364418567No Hit
ACCAACGGGGACCTGTTCAATACATAATCCTACTTGCCTGACTTACTCTTTC1610.11544032237247788No Hit
GTAGGCGGGCATGAAAAACAAATGCAATGTTTGGATCTTAGTTTGAACAAAT1600.11472330173662398No Hit
ATCATAGGGAAGCCATTCTAATATTATTAAAAATGCATTGAAAACAGCATTA1590.11400628110077007No Hit
ATGCAGGGGAGACCAAACCATTTGTATCTGGCATCACTTACTAACACACGAC1570.11257223982906227No Hit
ATTTAAGGGCAGCAATCTGATATTTTTATGTGAATCATCTTTGGAATTCGTG1560.11185521919320839No Hit
AAGGCTGGGGATGGCAGGAGTCAGAAATCACACATGCTGCCCAGCAGCAGAG1560.11185521919320839No Hit
CTCCATGGGGGCTGGTGAGGTAGAAGGGAGTATATCAAATACCTGTTCTGTT1560.11185521919320839No Hit
CTCGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1540.11042117792150058No Hit
CTGACTGGGAGACTTTGGCTTCATTTTTAATCTTTTTGGAGATTAAGTGTCT1500.10755309537808498No Hit
TGAGAAGGGAATATGTGAATCCAGAGATAAGTTTATACGGGAATTATTTGAA1490.10683607474223109No Hit
GCTTTAGGGTGTACTGAAGGGTCAAAGTTTCAATGTAGATCTTTAAGAGAAA1480.10611905410637718No Hit
AAAACCGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1480.10611905410637718No Hit
GCTACAGGGAAATGATAATAGAAACACAACATACCAAAACCTATGAAATACA1480.10611905410637718No Hit
TAACCTGGGATGGGAGGTTGTGTTGCTATACATTCTCACCAACAATCAGTTG1460.10468501283466937No Hit
TAGCTAGGGAGACTGACATGCACACATTTACTATTAAAACTACAACAGTAAT1460.10468501283466937No Hit
TACATGGGGTGTACAAGTTGTTATTGTTGAACATACTTCAAAAATAATGTGC1460.10468501283466937No Hit
CACACTGGGGAGAAATTAACAGAAAAGCTAGAGCCTCCAATCAGAAAAGATC1460.10468501283466937No Hit
TCAAGTGGGATGGTATGGCAGCCATTCTTTTATCTATTTAACCAAGAGGATG1450.10396799219881549No Hit
TGTATTGGGCTTGTTGTGTTTTACGATTTTACCATCTATGTATGTATCCCTA1450.10396799219881549No Hit
TTAGCGGGGACTGTGTAACAAGTCACTCAGTTTTATTTTAACTTTAGACGGT1450.10396799219881549No Hit
TGCTTGGGGCTGAGCCATACCAAGTTTACCAATGCTGTGGGAAAAAATCCTC1450.10396799219881549No Hit
TTGCTTGGGTGGGTGTGGTGGCTCACACCTGTAATTTCAGCACTTGGGACGC1440.10325097156296159No Hit
TTGAATGGGCTGTACCACGTGCAGATCAATTAATGTGATTCAGTATATCAAC1440.10325097156296159No Hit
ACCAGGGGGGGAAGTGAGTGATCGAAAGCATGGCGTCGGTGGTGTTGGCGCT1430.10253395092710768No Hit
TTTTATGGGACATTTAAAAAACACTGGTGTTGCTCTTTCCAGTTAGTGACAT1430.10253395092710768No Hit
GTTTCAGGGTGCCCAGCCCAGGGCGATGCCTGGAAGCCTACTGACATTGCAG1420.10181693029125378No Hit
ACACGGGGAGTTTTAGAAGATGAATTTGTTTTTGTTCCAAGGGTTTTTAAGT1410.10109990965539987No Hit
ACGCATCGGGAAAAGCCAGCTCTTGCCATCTATCTTGCATTAAAAGGAATTC1410.10109990965539987No Hit
AAGAACAGGGTGACAATTACAAACTCGTATAAATGCTTTGAAAGAAAGGTTC1400.10038288901954599No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)