FastQCFastQC Report
Sat 31 Dec 2022
SRR5848757.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848757.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138003
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC7270.526800142025898No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT6370.46158416846010597No Hit
ACAGTGGGGGATCTAAACATGGGTGGTGGAAGAACTGGCCTGTGATGGCCAT1640.11883799627544328No Hit
TTTCATGGGAGAGCACTCTATCTAAATAACAGCAGAATACACGTTCTTCTCA1620.1173887524184257No Hit
AATGCAGGGAATTTAAATTAGCAAAGAAAGGTGAGAAAAAAATCCCTTTGGT1590.11521488663289929No Hit
TGATTTGGGCGATCCTAGGAATGGGAAGTTATCTCTTTTATAATATTTTAAG1570.11376564277588168No Hit
GCACTCGGGAACCTAAGTGTCAATCAGTGGGTTAATGGATAAAGAAAATTGT1540.11159177699035529No Hit
GCTACAGGGAAATGATAATAGAAACACAACATACCAAAACCTATGAAATACA1500.10869328927632008No Hit
GTAGGCGGGCATGAAAAACAAATGCAATGTTTGGATCTTAGTTTGAACAAAT1500.10869328927632008No Hit
TTCAATGGCTTTGATTACTTTTTAATCATTGGCTTCAGTCTGTGGGAACTAA1480.10724404541930248No Hit
AGCTGAGGGCCAGGATTGCAGGTGTGAGCCACCGCACCTGGCAACCTTTTTC1470.10651942349079369No Hit
ATCTACGGGGTGAGAGCCATTATAAGTGAAATCTAAAAATTGATCGTTTTCA1470.10651942349079369No Hit
TTGCTAGGGAGACCTTGACTTTGAGAAGGTCATGCATACAAGGAAGAGGCAT1470.10651942349079369No Hit
ACCAACGGGGACCTGTTCAATACATAATCCTACTTGCCTGACTTACTCTTTC1460.10579480156228487No Hit
ATTGTGGGGACCAAGCAGAGACCTCTGCCTCACACACAATCAGACTGATGAT1460.10579480156228487No Hit
GGCTCAGGTCTTATCAATCTGACATCCCAAAATTGTCACATCTCCTGCAGAT1460.10579480156228487No Hit
ATCATAGGGAAGCCATTCTAATATTATTAAAAATGCATTGAAAACAGCATTA1450.10507017963377607No Hit
GTAGCTTGGGAGAATGGCGATTATTAAAAAGTCAAGAAACAATAGATGTGAG1450.10507017963377607No Hit
AAAGTAGGGATGAAACTCAAATTTCAATTTCTACAAATAGATTCCATCATTT1440.10434555770526728No Hit
TCTGATGGGAGATTTTCAGAACTTGAAGACTATTTTCTAATTTCTATTTTTT1440.10434555770526728No Hit
CGACCTGGGAAACCATAACAATTATAAAGATAACATCAGAAAAAATCCTTCT1440.10434555770526728No Hit
GTGATTGGGCAGCCTGTCCATGGATACTTGGATTCCATTGTGTGTGTGTGTG1430.10362093577675849No Hit
CTTGGCGGGCTAGAACCAATGACAAACGACCCCGTGAACTACGTGAGGCAAG1430.10362093577675849No Hit
AGACAGGGGGCTTATGTGGAGTTCGTCGATGTTAGCTCAGTGCCTCTAGCAA1420.10289631384824967No Hit
CTCGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1420.10289631384824967No Hit
GGTGGTGGGATGACCTGCTTCTGTGTTTATGATCTTAAGTGATAAAGTCTTA1410.10217169191974088No Hit
TCTCTAGGGGAAAGGACTAGACTTGGTCTTTAAGCTCCCTTCCGATTCTAAC1410.10217169191974088No Hit
CTCCATGGGGGCTGGTGAGGTAGAAGGGAGTATATCAAATACCTGTTCTGTT1400.10144706999123207No Hit
GGTAGGGGCTGGTTTTTCTTCAACTTTTTGTATTGAAAATTTCAACTCTACA1390.10072244806272328No Hit
AGCTAGGGGGATCCACTCAGCCTAGGCAACCATTGATCTGCTTTTATCACCA1390.10072244806272328No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)