FastQCFastQC Report
Sat 31 Dec 2022
SRR5848750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116783
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT10030.8588578817122355No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC8610.7372648416293468No Hit
GCTGCAGGGCACCACTCTTTGACACTGTCTTAAAATCGATAGAGCGAGAGCT2230.19095245027101546No Hit
TTTGATGGGACTGATGATAATTGAGCATCTTTTCATGTGCTCATTAACTGTT2120.1815332711096649No Hit
GCATAGGGGTGGGAAAGAATAAAAAAGCATCTAGAAAGACACTCTACAAATT1840.15755717869895447No Hit
GCAATTCGGGGAAGAAGGAAATTGAGTTCCACAGAAGTTGAATGATTTAATC1800.15413202264028153No Hit
ATGCCAGGGGGGCTTCTCTAGCACAGCAACTTCAAAGCCAGCAAGAGAGACA1780.1524194446109451No Hit
GCTGCTGGGTACTGAGTGGGCTCATGGGTTCTTTAAGTTCCCCCTCTTAACG1620.13871882037625338No Hit
ACGTCTGGGATTTATAAATTCAGTGAAACTTTAACCAAAATCCCAAAGGAAT1610.13786253136158516No Hit
AGCCTGGGGACAATTTTTTAAAAAAACAGGTAACAATGAAAAATATGTAAAG1540.13186850825890756No Hit
ACACCAGGGGAAAGACCAGAGCCCTCATTCCAAAGCCCCCCACCCAGATGGA1540.13186850825890756No Hit
GCTAACGGGACTGAACTGGCCGCTGGGAGAAAAGCCAGATGACAGGGAGCTG1530.1310122192442393No Hit
CAATGCGGGGAGCAGGGGGCCGGAAGTGGTTCTCCGCCCCTGCCACTGGGCC1530.1310122192442393No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCCTGTCTCTTATAC1520.1301559302295711No Hit
TTAGCAGGGCAGTTATTTTAGTAACTTTATTTCTTTCAGTGATGACTTCAGT1520.1301559302295711No Hit
TGTAACGGCCCCCAGTCCCTTCCTTCCTCCCTGCAGATTGTCTCCTAGAGTC1460.1250181961415617No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGA1450.12416190712689348No Hit
TACTCTGGGGATATGCTCCTGGATGGGGACTGGGCTGTGCCCAGGGCCTCTG1450.12416190712689348No Hit
GCCCGCCTCGGCGTCCCAAGGGGCTGTGATTGCAGGCATGAGCTACCAAGCC1430.12244932909755701No Hit
GTACATGGGATGACACTGACACTGCTATTTTGAAACCTGGAGGATGGAAAGG1430.12244932909755701No Hit
AACTTAGGGGGAAGGAGTTCTAAGACTTGCTCCTGCTTTAATTGACGTTGCA1420.12159304008288876No Hit
GCGCAAGGGAGACGCAGCCGCCCTCGGCGTCCTCTGTAGCGGGCGACCTAGG1420.12159304008288876No Hit
GGCTGGGACGTCTGGTTATGGCACTGGATGGTTTGGTGAACTTTCTGTGTGA1390.11902417303888409No Hit
CTTCTTGGGATATGCCAGTTTTGTAATTTATCTTAACTTTAAAATGACTGGG1380.11816788402421584No Hit
GCCATCAGAAGCAGTATCTTGGATTAGTTTGTTTTAGCTCTTGCTCTGTCCT1360.1164553059948794No Hit
CTTAGTGGTAAAAATAGGAATGAATCTCTTAATTGCAGCTTGTGGAGAGTAT1360.1164553059948794No Hit
AGAAACGGGACATGTCTTATAAAATATACCAAGTGAAGTCTTTCAGAATGAA1360.1164553059948794No Hit
AATTTTGGGATGCTAATGTAAGTCTTGAGCATGGAACAGCAAAGTGAACTAA1350.11559901698021116No Hit
GACGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1340.11474272796554294No Hit
TCAGCGGGGCAGGGCTTGGTACAATGAGCTGAATTAGATGGCCTTATGAAAC1340.11474272796554294No Hit
GCTAGGAAAGGGGTTGCTGGTCAGCTGGGCTTGGCTTCAGGCTTAGAACTGG1340.11474272796554294No Hit
TCTCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1340.11474272796554294No Hit
AGTCGCGGGTCAACTGAAATAGAGATGGCTCCTGGTGGAGCACATTTGGGGC1340.11474272796554294No Hit
TACACAGGGGTGTAAAAGATACCTTAACTCCACCAAGAGGGGCTGCTGAGTT1330.11388643895087469No Hit
CAAATAGGGTGTCTCTCTTGGCTGGGACCTTACCAGTCATGTCAGCTTGAGC1330.11388643895087469No Hit
CTTTCAGGGACTGGTTCATAGAATATGTGCATATTCACCTTTAGATATAGAG1320.11303014993620647No Hit
TAGAATGGGACATAACACACAGTTTATTCAGCAGCCCCAGGGGAAAAAAGAC1320.11303014993620647No Hit
CAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1320.11303014993620647No Hit
TTAAGCGGGGGCAGTTATTACCAAAAATACCTAAAGATTTGGAACTACCTTT1310.11217386092153823No Hit
ATACAGGGAAAGGCCCACACTCAAAGATCTTCTACTCATTTCACTAACTTAC1310.11217386092153823No Hit
AACAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAA1300.11131757190687001No Hit
TACCTTGGGAGAATCTTGTAAACTAAACCAGTTTATTTTTATGGGATCTTCA1300.11131757190687001No Hit
CGAACAGGATTCTTAGCTTTTCTAAATTACTGAACTCACCAGCAACCTGGAA1300.11131757190687001No Hit
AGGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1280.10960499387753354No Hit
CACTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1280.10960499387753354No Hit
ACATTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1270.10874870486286532No Hit
TATAGCGGGATAATGAAACTATCTACAATTCTTGTTTTAGCACATCTGTTAT1260.10789241584819709No Hit
CCAGCCTGGGTGACAGAGCAAGGCCTTGTCTCTAAAAAAATTGTTTTAATTA1250.10703612683352887No Hit
CAATCAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1240.10617983781886062No Hit
TTATGTGGGCTGAAAAAAAATTATTTTCCAAAATGTTCTGTTATTTAAAGTC1240.10617983781886062No Hit
TTGTCTGGGAACAGCTGTTTTGATGATGCTTGGCAACATGTTGGTATTTTTG1230.1053235488041924No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCCTGTCTCT1230.1053235488041924No Hit
ACTTGTGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1210.10361097077485593No Hit
TGGCCTGGGAGCCCTTGCTGGTATCAAGGGCAAATTGCTTTGGTTTTTTATG1210.10361097077485593No Hit
CTGAGGAGGGATGTTTATGCACCTAATAACAGAGCTTCAAAACACATGAAAC1200.10275468176018769No Hit
ACTTAGGGATCGTGCCATTGTATTCCAGCCTCGGCAACACAGCAGGACTGTG1180.10104210373085125No Hit
AACTGAGGGACACACCACCATGCCTGGCTGATTTTCGTTATTTTTAGTAGAG1180.10104210373085125No Hit
TTAGCAGGGTTATCACTGGTCATTAGAGAAATGCAAATCAAAACCACAATGA1180.10104210373085125No Hit
TTGGCTGGGGAGAGTCTGGTTCTTGACATGAGATGCCCTTCAGACATCTCTT1170.100185814716183No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)