FastQCFastQC Report
Sat 31 Dec 2022
SRR5848749.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848749.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117332
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT9740.8301230695803361No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC8630.7355197218150206No Hit
TTTGATGGGACTGATGATAATTGAGCATCTTTTCATGTGCTCATTAACTGTT2350.20028636689053284No Hit
GCTGCAGGGCACCACTCTTTGACACTGTCTTAAAATCGATAGAGCGAGAGCT1930.164490505573927No Hit
ATGCCAGGGGGGCTTCTCTAGCACAGCAACTTCAAAGCCAGCAAGAGAGACA1890.16108137592472643No Hit
AGCCTGGGGACAATTTTTTAAAAAAACAGGTAACAATGAAAAATATGTAAAG1860.158524528687826No Hit
GCAATTCGGGGAAGAAGGAAATTGAGTTCCACAGAAGTTGAATGATTTAATC1750.14914942215252447No Hit
ACGTCTGGGATTTATAAATTCAGTGAAACTTTAACCAAAATCCCAAAGGAAT1740.14829713974022432No Hit
GCTGCTGGGTACTGAGTGGGCTCATGGGTTCTTTAAGTTCCCCCTCTTAACG1740.14829713974022432No Hit
TACTCTGGGGATATGCTCCTGGATGGGGACTGGGCTGTGCCCAGGGCCTCTG1670.14233116285412334No Hit
GCCATCAGAAGCAGTATCTTGGATTAGTTTGTTTTAGCTCTTGCTCTGTCCT1650.14062659802952307No Hit
CTTCTTGGGATATGCCAGTTTTGTAATTTATCTTAACTTTAAAATGACTGGG1640.13977431561722292No Hit
AGTCGCGGGTCAACTGAAATAGAGATGGCTCCTGGTGGAGCACATTTGGGGC1640.13977431561722292No Hit
AATTTTGGGATGCTAATGTAAGTCTTGAGCATGGAACAGCAAAGTGAACTAA1610.1372174683803225No Hit
GCATAGGGGTGGGAAAGAATAAAAAAGCATCTAGAAAGACACTCTACAAATT1570.13380833873112194No Hit
TACCTTGGGAGAATCTTGTAAACTAAACCAGTTTATTTTTATGGGATCTTCA1570.13380833873112194No Hit
CGAACAGGATTCTTAGCTTTTCTAAATTACTGAACTCACCAGCAACCTGGAA1560.13295605631882182No Hit
ACACCAGGGGAAAGACCAGAGCCCTCATTCCAAAGCCCCCCACCCAGATGGA1520.12954692666962125No Hit
GACGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1510.1286946442573211No Hit
AACAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAA1510.1286946442573211No Hit
CAATGCGGGGAGCAGGGGGCCGGAAGTGGTTCTCCGCCCCTGCCACTGGGCC1510.1286946442573211No Hit
TAGAATGGGACATAACACACAGTTTATTCAGCAGCCCCAGGGGAAAAAAGAC1490.1269900794327208No Hit
TGTAACGGCCCCCAGTCCCTTCCTTCCTCCCTGCAGATTGTCTCCTAGAGTC1460.12443323219582042No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCCTGTCTCTTATAC1440.12272866737122012No Hit
GCCATAGGGTGGGAAATTGGATATGAAAGAAATACACACCTACATGGAAATG1440.12272866737122012No Hit
ACATTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1420.12102410254661986No Hit
ACGCCTGGGGCTGCCACAAGAACTGCTTGGTATTGCCTACATGTGTTGTCCT1400.11931953772201956No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGA1390.11846725530971942No Hit
TTAGCAGGGCAGTTATTTTAGTAACTTTATTTCTTTCAGTGATGACTTCAGT1390.11846725530971942No Hit
CACACGGGGGAGGCTGGTAAGTCTAATCTGCAGAGTCAGCAAGCAGGCTGGA1390.11846725530971942No Hit
GCACCGTTTTGCATTCCCACCAGCAATGAATGAGAGCTCCTCTTTCTACACA1390.11846725530971942No Hit
TTAAGCGGGGGCAGTTATTACCAAAAATACCTAAAGATTTGGAACTACCTTT1380.11761497289741929No Hit
TTGTCTGGGAACAGCTGTTTTGATGATGCTTGGCAACATGTTGGTATTTTTG1340.11420584324821872No Hit
AACTAAGGGGTGGTCCCAGCTACTTGGGAGGTTGAGGTAGGAGGACCACTTT1330.11335356083591859No Hit
TTTAAGGGGAGATATACAAGGCTGTTTTAGTATTCCAAAATTAATTCATGAA1330.11335356083591859No Hit
GCAGCGGGAAGCTTAGTTTGGTTAGGGAAAGAAAGTTGGTATCAACGAGAGC1320.11250127842361846No Hit
ACTAGCGGGCCTGCAATTGTGTCTTCATTCTGATTAGTTTATTCCATTTAGA1320.11250127842361846No Hit
TCAGCGGGGCAGGGCTTGGTACAATGAGCTGAATTAGATGGCCTTATGAAAC1310.11164899601131832No Hit
GTACATGGGATGACACTGACACTGCTATTTTGAAACCTGGAGGATGGAAAGG1310.11164899601131832No Hit
GCGCAAGGGAGACGCAGCCGCCCTCGGCGTCCTCTGTAGCGGGCGACCTAGG1310.11164899601131832No Hit
CAAATAGGGTGTCTCTCTTGGCTGGGACCTTACCAGTCATGTCAGCTTGAGC1300.11079671359901816No Hit
CTTAGTGGTAAAAATAGGAATGAATCTCTTAATTGCAGCTTGTGGAGAGTAT1300.11079671359901816No Hit
GCTAGGAAAGGGGTTGCTGGTCAGCTGGGCTTGGCTTCAGGCTTAGAACTGG1290.10994443118671803No Hit
GCCCGCCTCGGCGTCCCAAGGGGCTGTGATTGCAGGCATGAGCTACCAAGCC1280.10909214877441789No Hit
ACTTAGGGATCGTGCCATTGTATTCCAGCCTCGGCAACACAGCAGGACTGTG1270.10823986636211776No Hit
TACTGTGGGCAGTAAAATTTGGCATCCATGATAATCTTATCTCATTTCAACT1270.10823986636211776No Hit
TATAGCGGGAGCTGAAACCAGCAGAATTGAAAACTCTGGCAATAAAACATGG1260.1073875839498176No Hit
TGTTGGGGGAATTTAAAAAAAGCTAGTGCAAGTAGAATCAGAATTTTAAGGC1260.1073875839498176No Hit
AACTTAGGGGGAAGGAGTTCTAAGACTTGCTCCTGCTTTAATTGACGTTGCA1260.1073875839498176No Hit
TTGGCTGGGGAGAGTCTGGTTCTTGACATGAGATGCCCTTCAGACATCTCTT1240.10568301912521733No Hit
TTTCTTGGGGTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACT1240.10568301912521733No Hit
TTATATGGGATGCTATATAATATAATTGACAAAATATGCTAAAAGGCAAAAA1240.10568301912521733No Hit
TTATGTGGGCTGAAAAAAAATTATTTTCCAAAATGTTCTGTTATTTAAAGTC1240.10568301912521733No Hit
GGCTGGGACGTCTGGTTATGGCACTGGATGGTTTGGTGAACTTTCTGTGTGA1230.10483073671291719No Hit
CATCTTGGGGAACTACCACCAGGACTCAGAGGCCGCCATCAACCGCCAGATC1230.10483073671291719No Hit
GCTAACGGGACTGAACTGGCCGCTGGGAGAAAAGCCAGATGACAGGGAGCTG1230.10483073671291719No Hit
CATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC1230.10483073671291719No Hit
CTCTCAGGGACATACTTTGAGGATTGACTTTATATAAGGTGCCCTGTAGAAA1230.10483073671291719No Hit
GCTTAGGGGATTGATTACTAAAATAAGTTAATATTAAGCAAGGTGCCGGTTG1230.10483073671291719No Hit
ATTCAAGGGAATGCCTGGAATACACATATTTTAGATTCTTAAAAGACATCTT1220.10397845430061706No Hit
GTCATAGGGATGTAGATCGAGTGACATTGCATAGGTTGAACAGGTGTTTTAT1220.10397845430061706No Hit
TTCTCTGGGGATACTAAAAAGGGCAAATCAGCTAAGAGCTCTATAATTTCTA1220.10397845430061706No Hit
ACTGCTGGGATCAAAAAATTTTTTGCAGTAACTTTATTTCTCTTTTTATGGT1220.10397845430061706No Hit
CAATCAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1210.10312617188831691No Hit
ACAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1210.10312617188831691No Hit
GTGGCCGGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTC1200.10227388947601677No Hit
AAGCGAGCGGGAAGGATTTTTATCTCTTTATCTGGGCACTTCTTAGAATGGA1200.10227388947601677No Hit
AAGACTGGGCTTTCACTACGTGGTAATGAAGATGGCCCCCAGCAACTCCAAT1190.10142160706371664No Hit
CACTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1190.10142160706371664No Hit
ATGTGCGGGATGACTGAGACAGCTTTTGGCTCCGCATATAAAACCAAGAAGG1190.10142160706371664No Hit
CAGAGGGGGGCTCGGCGGCGGCGGGCGCGGGAAGATGGCGGCAGCGGCGGCG1190.10142160706371664No Hit
AATTGAGGGCTCGCAGGCACCCCCAGGTTATGTGGACAGAGCTAAGCCCAAA1190.10142160706371664No Hit
GCACTTGGGTAGCCTCCAGAACTGTGAGAGAATACATTTCTGTTGTTTTAAG1180.10056932465141649No Hit
AGAGTAGGGCAAGTGATGCTTACAAAAAAGCCTGGGGCAATAATCAGGACGG1180.10056932465141649No Hit
TAATTTGGGTGGGCTTTATTGGCCCTTGCTCAGTAGAAGGTGCTGAGAACAG1180.10056932465141649No Hit
ACGTCAGGGGTAGAGGAAACTTCTCTCTGAGCATTGTTTTAAGGGTGCTTTT1180.10056932465141649No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)