FastQCFastQC Report
Sat 31 Dec 2022
SRR5848748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116388
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT10250.880674983675293No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC8820.7578100835137643No Hit
GCTGCAGGGCACCACTCTTTGACACTGTCTTAAAATCGATAGAGCGAGAGCT2130.183008557583256No Hit
TTTGATGGGACTGATGATAATTGAGCATCTTTTCATGTGCTCATTAACTGTT2000.17183902120493522No Hit
AATTTTGGGATGCTAATGTAAGTCTTGAGCATGGAACAGCAAAGTGAACTAA1770.15207753376636765No Hit
GTACATGGGATGACACTGACACTGCTATTTTGAAACCTGGAGGATGGAAAGG1600.13747121696394818No Hit
TTAGCAGGGCAGTTATTTTAGTAACTTTATTTCTTTCAGTGATGACTTCAGT1580.13575282675189884No Hit
CTTCTTGGGATATGCCAGTTTTGTAATTTATCTTAACTTTAAAATGACTGGG1560.13403443653984948No Hit
ACGTCTGGGATTTATAAATTCAGTGAAACTTTAACCAAAATCCCAAAGGAAT1560.13403443653984948No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGA1510.12973846100972608No Hit
TGTAACGGCCCCCAGTCCCTTCCTTCCTCCCTGCAGATTGTCTCCTAGAGTC1510.12973846100972608No Hit
ATGCCAGGGGGGCTTCTCTAGCACAGCAACTTCAAAGCCAGCAAGAGAGACA1480.12716087569165205No Hit
TACACAGGGGTGTAAAAGATACCTTAACTCCACCAAGAGGGGCTGCTGAGTT1460.1254424854796027No Hit
AGTCGCGGGTCAACTGAAATAGAGATGGCTCCTGGTGGAGCACATTTGGGGC1450.12458329037357803No Hit
ACACCAGGGGAAAGACCAGAGCCCTCATTCCAAAGCCCCCCACCCAGATGGA1440.12372409526755336No Hit
ACTTAGGGATCGTGCCATTGTATTCCAGCCTCGGCAACACAGCAGGACTGTG1430.12286490016152868No Hit
TACTCTGGGGATATGCTCCTGGATGGGGACTGGGCTGTGCCCAGGGCCTCTG1430.12286490016152868No Hit
GCAATTCGGGGAAGAAGGAAATTGAGTTCCACAGAAGTTGAATGATTTAATC1400.12028731484345465No Hit
CGAACAGGATTCTTAGCTTTTCTAAATTACTGAACTCACCAGCAACCTGGAA1390.11942811973742998No Hit
ACAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1380.1185689246314053No Hit
AGCCTGGGGACAATTTTTTAAAAAAACAGGTAACAATGAAAAATATGTAAAG1370.11770972952538063No Hit
AATTGAGGGCTCGCAGGCACCCCCAGGTTATGTGGACAGAGCTAAGCCCAAA1350.11599133931333126No Hit
GCTGCTGGGTACTGAGTGGGCTCATGGGTTCTTTAAGTTCCCCCTCTTAACG1350.11599133931333126No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCCTGTCTCTTATAC1340.1151321442073066No Hit
CAAATAGGGTGTCTCTCTTGGCTGGGACCTTACCAGTCATGTCAGCTTGAGC1340.1151321442073066No Hit
GCACCGTTTTGCATTCCCACCAGCAATGAATGAGAGCTCCTCTTTCTACACA1340.1151321442073066No Hit
GCATAGGGGTGGGAAAGAATAAAAAAGCATCTAGAAAGACACTCTACAAATT1330.11427294910128193No Hit
CAATGCGGGGAGCAGGGGGCCGGAAGTGGTTCTCCGCCCCTGCCACTGGGCC1330.11427294910128193No Hit
AACTGTGGGAGAGGATTGAACTATACCCTGTCTGTACTGTACATAGAAAATC1320.11341375399525723No Hit
CGCCCGGGGCTTGCGGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCT1320.11341375399525723No Hit
TTATATGGGATGCTATATAATATAATTGACAAAATATGCTAAAAGGCAAAAA1320.11341375399525723No Hit
GACGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1310.11255455888923256No Hit
ATTCAAGGGAATGCCTGGAATACACATATTTTAGATTCTTAAAAGACATCTT1300.1116953637832079No Hit
CATCTTGGGGAACTACCACCAGGACTCAGAGGCCGCCATCAACCGCCAGATC1290.11083616867718321No Hit
GCCATCAGAAGCAGTATCTTGGATTAGTTTGTTTTAGCTCTTGCTCTGTCCT1290.11083616867718321No Hit
GTTTGAGGGCTGCGATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCT1280.10997697357115854No Hit
ATACAGGGAAAGGCCCACACTCAAAGATCTTCTACTCATTTCACTAACTTAC1280.10997697357115854No Hit
TTATGTGGGCTGAAAAAAAATTATTTTCCAAAATGTTCTGTTATTTAAAGTC1280.10997697357115854No Hit
TATAGCGGGATAATGAAACTATCTACAATTCTTGTTTTAGCACATCTGTTAT1270.10911777846513387No Hit
TGCTGCAGGGCTTTTCCTCTAGGCAGAGAAGAGGCGATGGCGGCGATGGCAT1260.10825858335910918No Hit
AACAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAA1260.10825858335910918No Hit
AGCTCTGGGGAGACATCAAAAATCAAAGACTAGATGAGCTCATTCATGTGAA1260.10825858335910918No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAAACTCGTATGC1260.10825858335910918No Hit
AGGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1250.10739938825308451No Hit
GCGCAAGGGAGACGCAGCCGCCCTCGGCGTCCTCTGTAGCGGGCGACCTAGG1250.10739938825308451No Hit
GTGGCCGGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTC1240.10654019314705983No Hit
TACTGTGGGCAGTAAAATTTGGCATCCATGATAATCTTATCTCATTTCAACT1240.10654019314705983No Hit
GCTAACGGGACTGAACTGGCCGCTGGGAGAAAAGCCAGATGACAGGGAGCTG1230.10568099804103516No Hit
CATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC1230.10568099804103516No Hit
TTTAGGGTAAGGGCCTCCCCAGGGTTATCAGGTCCACAAAGGGTCTTGGGAT1220.10482180293501049No Hit
ACGCCTGGGGCTGCCACAAGAACTGCTTGGTATTGCCTACATGTGTTGTCCT1220.10482180293501049No Hit
CTTAGTGGTAAAAATAGGAATGAATCTCTTAATTGCAGCTTGTGGAGAGTAT1220.10482180293501049No Hit
TTCTCAGGGGTGATTCTGCTACAATTCTGTTTCTTGACTTGGATGCTGGTTT1210.1039626078289858No Hit
CACTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1210.1039626078289858No Hit
ACTTGTGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1210.1039626078289858No Hit
ACTAGCGGGCCTGCAATTGTGTCTTCATTCTGATTAGTTTATTCCATTTAGA1210.1039626078289858No Hit
CTTTCAGGGACTGGTTCATAGAATATGTGCATATTCACCTTTAGATATAGAG1200.10310341272296113No Hit
CAATCAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1200.10310341272296113No Hit
CTTTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1200.10310341272296113No Hit
AACTTAGGGGGAAGGAGTTCTAAGACTTGCTCCTGCTTTAATTGACGTTGCA1190.10224421761693646No Hit
TGAGATGGGGTTCGCGAGCCCGCCCGAACAGACGCTGGAGTGGAACGACGCC1190.10224421761693646No Hit
TCAGCGGGGCAGGGCTTGGTACAATGAGCTGAATTAGATGGCCTTATGAAAC1180.10138502251091178No Hit
GCCATAGGGTGGGAAATTGGATATGAAAGAAATACACACCTACATGGAAATG1180.10138502251091178No Hit
GGCTGGGACGTCTGGTTATGGCACTGGATGGTTTGGTGAACTTTCTGTGTGA1170.10052582740488711No Hit
CTTTACGGGTCCATGAAATCTTCACAATTTATGTTCTTCTGCCGTGGCTTCA1170.10052582740488711No Hit
GTCATAGGGATGTAGATCGAGTGACATTGCATAGGTTGAACAGGTGTTTTAT1170.10052582740488711No Hit
AGAGTAGGGCAAGTGATGCTTACAAAAAAGCCTGGGGCAATAATCAGGACGG1170.10052582740488711No Hit
CTATTGGGGCTCCTCCCTTACCCTCCTCTGCTGGGAACCTAGCACACCTGAA1170.10052582740488711No Hit
CTTCGCGGGGTAATTAGCAGGATTTAGTTTAAAGTAAGTCTTTCCCAAATAC1170.10052582740488711No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)