FastQCFastQC Report
Sat 31 Dec 2022
SRR5848744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207052
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC13880.6703630005988834No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT12750.6157873384463807No Hit
GCAGCATGTGAACAGTAATCAGTGCCTGGATAAAGCCACAGAAGAGGATAGC2560.1236404381508027No Hit
TGTGCTGGGTAGTAAAACCCAGTTATATGCCTCTATCTGATATAGCAAACAT2510.12122558584316984No Hit
TGCGCTAGGGGAACTGACACTAGTGATGTCCCATATTCTCAGGACCTTGATC2460.11881073353553696No Hit
CTCAGAGGGCAAGTACTGGCTTTATAAAGAAGAAGGCGAGAGGAGACACAGG2440.11784479261248383No Hit
TTCTAGGGAAGATAATTAAATGAAATACTTGACAACATTTTTCATTCCTGCT2410.1163958812279041No Hit
CGATTCGGGATACTTAAACGTGAAAGGTGACTAAATGCGGGGAAGAAAGATT2410.1163958812279041No Hit
GACACAGGGATCTTTGGAAGCCTTGAGTTCAGAATCTGCTGCTCTGGAATAT2370.1144639993817978No Hit
TGTCAGGGGTAACTGAAAATCTTAAGTGTAGAGATAGCTTACTAGCTAATGG2370.1144639993817978No Hit
CTGTAGACGGGCGGCCACCACCACCCCACAACACCCCCAAAAGGAACAGTTT2310.11156617661263837No Hit
TAGCAGGGGAAACTATAGCAGCTCATGCTCACTTTCTAGGAGTCTTGGGAGT2280.11011726522805866No Hit
GCCAGAGGGATTCATTAAAATGTACAGCAGTAGAAATAAGAAACATTATTGA2270.10963429476653207No Hit
ATGAGGAGGGGATCATGACAACGTCAGGTATGGCATTTCAAATATAGATACA2270.10963429476653207No Hit
TGCGCAGGGAACATGCGACCATTTGACTATGTTACAACCAAGAGATCCAAAG2250.10866835384347893No Hit
ACTTAGGGGGGTCAGGTGGCCTTGGCCAACCAGTGCCTCTCCCAAGCTGATG2220.10721944245889921No Hit
CTATCCGGGTTGAAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAA2210.10673647199737264No Hit
TGTAAAGGGAGTAGTAATAATAACTAATACATATTAAGCATTTTTCATACAA2210.10673647199737264No Hit
TGATTGGGAGCACTTGACATGCGGTCACCGGTGGCAGCGCGGTGTGTTGAAG2190.1057705310743195No Hit
CACAGCAGGAAGGAAGCAGTGTAAATGGAGGTACACGGGAAGCACTTCCTTT2180.10528756061279293No Hit
ATGTTTGGGGGGAAGAGTACAGAGGTGGCAGAATGAAGCTAGAGTGGGAAGG2180.10528756061279293No Hit
TTTAATGGGGCATACAGAAAAGAGAGTAACCCAATAGTGAGGAAGAAATTGA2170.10480459015126634No Hit
TTAGCCGGGAGTCAAAAGGCCCTTGATTAATATCTGTCTTCTTGAGTAATTT2160.10432161968973977No Hit
TCCTATTTGGTAGGTAATACCCTGGAGTAGATAAAAAGTTAAGTTATAGATG2130.10287270830516007No Hit
AAGACTGGGGGATTCTATGTCCCTAATGACTAGTGATGTTGAGCATTTTCTA2120.10238973784363348No Hit
CCAGGCCCAGAGAACTCTTCCACAAACAAGATGAGAGCCACTCGGGAAAAGA2120.10238973784363348No Hit
TTAGGAGGGGGGCGCGCGAGGCTGTTGTGCTCCCGGCTCTCGTGTTTCCCCT2120.10238973784363348No Hit
ACGCGTGGGTCAGCTTGCTCCCTTCTCTTTTAATTTCAGCTATCATTTTTAG2120.10238973784363348No Hit
GTAGTGGGGGCGCGGACGGGGCGGGCCCGCGTGGCGCACGCAGAGCCACTTC2110.10190676738210691No Hit
GAATTTGGGGCAGTTGCTTATAGTTAGTGTCTTTAATTAAGTTTCCATCATT2100.10142379692058033No Hit
AAAGTAGGGAAGAAGAGTCGGGCTCCAGCAGTGCTTCAGTGAGAACCCAGCC2100.10142379692058033No Hit
TTCCAGGGGGAATTCCTCAGCACCAGATATTCAGTATTCATCATAGCCATTG2100.10142379692058033No Hit
TGATGCAGGGAGGAGGTAAAAAACTAAGGAGCACTGTCCAAAGAAGTACAGA2090.10094082645905376No Hit
TTCAATGGGCAAAAAATTGTTGAAGACGTGACTTCATAGCAATATATAGAGA2090.10094082645905376No Hit
CAGAATGGGAGGGTTGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGCG2090.10094082645905376No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)