FastQCFastQC Report
Sat 31 Dec 2022
SRR5848742.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848742.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205248
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGC12340.6012238852510134No Hit
CTTATACACATCTCCGAGCCCACGAGACACCATAGAATCTCGTATGCCGTCT11970.5831969130028063No Hit
CTCAGAGGGCAAGTACTGGCTTTATAAAGAAGAAGGCGAGAGGAGACACAGG2580.12570159027128158No Hit
TTAGCCGGGAGTCAAAAGGCCCTTGATTAATATCTGTCTTCTTGAGTAATTT2390.11644449641409417No Hit
TGCGCTAGGGGAACTGACACTAGTGATGTCCCATATTCTCAGGACCTTGATC2350.11449563454942314No Hit
GCCAGAGGGATTCATTAAAATGTACAGCAGTAGAAATAAGAAACATTATTGA2300.11205955721858435No Hit
TGTGCTGGGTAGTAAAACCCAGTTATATGCCTCTATCTGATATAGCAAACAT2250.10962347988774555No Hit
AAGACTGGGGGATTCTATGTCCCTAATGACTAGTGATGTTGAGCATTTTCTA2220.10816183348924227No Hit
CTGTAGACGGGCGGCCACCACCACCCCACAACACCCCCAAAAGGAACAGTTT2220.10816183348924227No Hit
GGGGTGGGGATACCAAGATGTTATGAGGTTGAACCCGACACTCCTTCTCCTT2210.10767461802307451No Hit
TTCTAGGGAAGATAATTAAATGAAATACTTGACAACATTTTTCATTCCTGCT2190.10670018709073902No Hit
AGCTTAGGGAACTCCAACATATGATGAAAACCCCATGAAGGCCTCGAAAAAG2180.10621297162457125No Hit
ACAGCTGGGCAGAGAAAAGATAAACACAATTTACATATAAAGATATGTTATT2170.10572575615840349No Hit
CGATTCGGGATACTTAAACGTGAAAGGTGACTAAATGCGGGGAAGAAAGATT2160.10523854069223575No Hit
TGCGCAGGGAACATGCGACCATTTGACTATGTTACAACCAAGAGATCCAAAG2150.10475132522606798No Hit
CTATCCGGGTTGAAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAA2150.10475132522606798No Hit
ATGAGGAGGGGATCATGACAACGTCAGGTATGGCATTTCAAATATAGATACA2140.10426410975990022No Hit
GCCGGCGGGGCGCTCCCAGTCGCTGAGTGCCTGAGCCGGGAAGCAGTTGCTG2120.1032896788275647No Hit
ATTTCAGGGGAGTCGCGGGGATGAGCTCTGGGCAGGACGGCGGGCCGAGGAG2110.10280246336139695No Hit
GCGCAGGGTCTCAAAAGGAGACATGTATGCAGCCAACAGACACATGAAAAAC2110.10280246336139695No Hit
GCAGCATGTGAACAGTAATCAGTGCCTGGATAAAGCCACAGAAGAGGATAGC2110.10280246336139695No Hit
TAGCAGGGGAAACTATAGCAGCTCATGCTCACTTTCTAGGAGTCTTGGGAGT2110.10280246336139695No Hit
ATTTGTGGGGTAGCCTTCAAATTTTATGTATCCAAATTTTGAAGAAGCTGTT2090.10182803242906142No Hit
GCATCGTGGGGGGTGCGCGGGCGCAGAGCCTGGAATCCGGGCGCTGGGGCCC2060.10036638603055814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)