FastQCFastQC Report
Sat 31 Dec 2022
SRR5848729.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848729.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150335
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC6930.4609704992184122No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT5800.38580503542089334RNA PCR Primer, Index 40 (95% over 22bp)
GAACATGGGATTTGGTTTCTTTAAAAAAGATCTGAAGCAAATGTAACATCAA1870.12438886486846043No Hit
GCAGTTGGGAAGATGATTGTCAGTACATTAAACTTTTCAATCCCATTATGCA1690.11241560514850168No Hit
AGCCGTGGGACAGAAGGGTAAGTTGGAATTCATAGTTTAACTGTGACTGCCT1640.10908969967073537No Hit
GTATTGGGGATGAGGATGTGTTCCAAACAAGGAAACTAAAAATATGTTTCCT1640.10908969967073537No Hit
GGGACAGGGACATATGGCAAAGCTAATAGCTGAAAACCAAAGAAAATATCAA1610.10709415638407557No Hit
ACGCGAGGGCTGCTTTTTGTAAAGCGTAAAAACATCACAAAGTAGGTCATTC1570.10443343200186252No Hit
CCAACCGGGGACCCCCGGGTGTGCTCTCAGCGCAAGTGGTCCTAGAACAGGA1540.10243788871520272No Hit
CAAGGTGGGCATCATAGTAAAATACCTAGACCCAAGTAAATATTTACTCTTC1520.1011075265240962No Hit
ACGAATGGGAGTGGCATTCTGACTCCATTAGTTTCTCATTCTTCTTCTGGGA1510.10044234542854291No Hit
GTAGCCGGGAATGTGGTATTCCCAAAGTTGAGTGTTTAGGATTATTATGATT1510.10044234542854291No Hit
CTCTCCGGGCATTCATGGATTGGGTTACCTTATCTTCCTGACCATTGAGAGT1510.10044234542854291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)