FastQCFastQC Report
Sat 31 Dec 2022
SRR5848728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149748
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC6550.4374015011886636No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT5780.3859817827283169RNA PCR Primer, Index 40 (95% over 22bp)
GAACATGGGATTTGGTTTCTTTAAAAAAGATCTGAAGCAAATGTAACATCAA1800.12020193925795337No Hit
TGCTCAGGGAAAGCAGTCTTGTTAAATTGACATAAATGGTAAACTTCAACAT1770.11819857360365414No Hit
TTCGTCGGGGATTCTTGTGACGTTAACAAAGGCAAAATTAGATGCTAGATAT1760.11753078505222106No Hit
AACCTAGGGGAGAGACCACTCTGTGGCTTTGTGAACTTGAGTGAGTCTTTTA1720.11485963084648877No Hit
GTGCCTGGGGGATCCATGCATCCTAACAAGTTTTCTGCTGCATCTGCTTCCT1710.11419184229505569No Hit
ACGAATGGGAGTGGCATTCTGACTCCATTAGTTTCTCATTCTTCTTCTGGGA1650.11018511098645725No Hit
GGGACAGGGACATATGGCAAAGCTAATAGCTGAAAACCAAAGAAAATATCAA1650.11018511098645725No Hit
AGCCGTGGGACAGAAGGGTAAGTTGGAATTCATAGTTTAACTGTGACTGCCT1630.1088495338835911No Hit
TGTTTAGGGACAGCAAGAGAATGGGAAAATAGACTAATACATAGAAACTAAG1630.1088495338835911No Hit
GCAGTTGGGAAGATGATTGTCAGTACATTAAACTTTTCAATCCCATTATGCA1610.10751395678072495No Hit
TCGATAAGGGCATGGTCTTTTAGCCTTTGAATTCCAGTTGCTCTTAGAAGTT1610.10751395678072495No Hit
TGACACGGGAATGTAATTAAAAAATAGGCAAAGAATATGAACAGACGCTTCT1600.10684616822929187No Hit
GTATACGGGATTCTTGTCAGTTTAGAAATGGACTGGATAAAACTTACTTGGT1590.1061783796778588No Hit
GCTCACGGGGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAG1580.10551059112642575No Hit
GTCTCGGGCGATCTCAATGCGGGATGACCCCAATACGCAAAACTAACCCCCT1580.10551059112642575No Hit
CAAGGTGGGCATCATAGTAAAATACCTAGACCCAAGTAAATATTTACTCTTC1560.10417501402355958No Hit
CCTTTTTGGGGAGTCCCTGAATTACAGTAGCAAACAGTTTGCAGAATCCTAC1550.10350722547212651No Hit
GTATTGGGGATGAGGATGTGTTCCAAACAAGGAAACTAAAAATATGTTTCCT1530.10217164836926035No Hit
CCGTATGGGGGGAAACAGGAATTTTTTTTGACGAGAGTCAAATGCTTATCAC1520.10150385981782728No Hit
CCAACCGGGGACCCCCGGGTGTGCTCTCAGCGCAAGTGGTCCTAGAACAGGA1520.10150385981782728No Hit
ACGTCTGGGAATACAATAAAAATAAATTATAAGAAAAGTGAAATTCCAAAAA1520.10150385981782728No Hit
TGCTACGGGGCAATTAACTGATGGAAGGAATTGGTTTCATGAGACGCTGTAT1510.1008360712663942No Hit
TTCACAAGGGAGGCCCTGATTTTGTTGTTTGTGATGAAGGCCATATTCTAAA1510.1008360712663942No Hit
ATGCCGGGCATTAATACCTACTTATTAAAACTAATGAAAAGGGTTTATATCA1500.10016828271496114No Hit
CTCTCCGGGCATTCATGGATTGGGTTACCTTATCTTCCTGACCATTGAGAGT1500.10016828271496114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)