FastQCFastQC Report
Sat 31 Dec 2022
SRR5848727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151021
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC6410.4244442825832169No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT5650.3741201554750664RNA PCR Primer, Index 40 (95% over 22bp)
GAACATGGGATTTGGTTTCTTTAAAAAAGATCTGAAGCAAATGTAACATCAA1890.1251481582031638No Hit
GGGACAGGGACATATGGCAAAGCTAATAGCTGAAAACCAAAGAAAATATCAA1840.12183736036710127No Hit
GTTAGGGACACTTACCTATTCTGGATATTTCATATAAATGGAATTGCACAAC1730.11455360512776368No Hit
ATGCCGGGCATTAATACCTACTTATTAAAACTAATGAAAAGGGTTTATATCA1720.11389144556055118No Hit
GCAGTTGGGAAGATGATTGTCAGTACATTAAACTTTTCAATCCCATTATGCA1700.11256712642612617No Hit
CAAGGTGGGCATCATAGTAAAATACCTAGACCCAAGTAAATATTTACTCTTC1560.10329689248515107No Hit
CCGTATGGGGGGAAACAGGAATTTTTTTTGACGAGAGTCAAATGCTTATCAC1560.10329689248515107No Hit
GTGTCGGGAATTATTTTTTGAAGCATTATTTTCCCAACACAAAAGAGCTTTT1550.10263473291793858No Hit
TTCGTCGGGGATTCTTGTGACGTTAACAAAGGCAAAATTAGATGCTAGATAT1540.10197257335072606No Hit
GTAGCCGGGAATGTGGTATTCCCAAAGTTGAGTGTTTAGGATTATTATGATT1530.10131041378351356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)