FastQCFastQC Report
Sat 31 Dec 2022
SRR5848721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72085
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT3790.5257681903308594RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC3280.45501838107789416No Hit
GATACAGGGCAGGAAGGATAAAGTTCCAAATGAGCTAAGCCTTTCAAAACTG1380.19144066033155305No Hit
AAAAGCGGGGTGCCTACCTTTCCCAAAGACTTACACATGTTCAAACAACGTT1270.1761808975515017No Hit
TGAATAGGGACTTTCAGCAAAGAAATTTATTTTGAGAATATGAATATTTCAG1210.1678573905805646No Hit
GCACCAGGGCGATCTTGGCATGGGATTAATTTCTATTTCTGCAAATTTGTCA1210.1678573905805646No Hit
GACTCTGGGCAGAAAAAGAATTGTAATGGCTACAGTTTCAGTTACTTCCATT1170.1623083859332732No Hit
AAACAGGGGTTTGTAGATGATTTTGATTTGCAAAGATTTGGAATTGATTTAT1110.15398487896233615No Hit
TTAGCGGGGGAGTCGCGGGTGCGCGCGGGCCGTGAGTGTGCGCTTTTGAGAG1110.15398487896233615No Hit
TGAACCGGGTGAAAGCACAAGCGTACCAAACGGGCAAGGACATCTCTACAAA1110.15398487896233615No Hit
GCAATTGGGTGTTAAGGGGAAAACTCAATAGTTCTGTTACTTAGGCTGTTAG1100.15259762780051328No Hit
CTGCTTGGGATACACCAAAGTTTTGACGGTTTACCATCATTCCATTTTGTAC1100.15259762780051328No Hit
CTTGTAGGGGAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGT1090.15121037663869044No Hit
GAAACTGGGAGAAATTAATCTTGATATAATACCATTAAATAAAATACAGAAC1070.14843587431504474No Hit
ATTAGTGGGCGCCCAGCACAATGAAGATCAAGATCACTGCTCCTCCCAAGCG1060.1470486231532219No Hit
AAAGTGGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGAATCACAGTACTTT1020.1414996185059305No Hit
AATTGCGGGTGTACAAATGTTTGTGTTTTCATTGTTACACTTTGGGGTTTTA1020.1414996185059305No Hit
TGCTTAGGGTCTTCGTGAGGGCATAGGGTCAAGTTGTCCTGGTTCTGCAAAA1010.14011236734410765No Hit
GAGCTGGGGGCGCTGGTGAACAGGACCCGTCGCCATGGGCCGTGTGATCCGT1000.1387251161822848No Hit
GTGGACGGGACTGGAAAAGACCCCAAAAAGGCAGTAGAGGAGATTAGTGTTT1000.1387251161822848No Hit
ACAAGCGGGTGTTACTGAAGAAGACAAGAGGATTCTTCGTGACATTGAGACT990.13733786502046194No Hit
CATCTTGGGGATGTGAATTTAATGACAAAGATGACTCTCATTTTCTTAGCAG990.13733786502046194No Hit
CTCAACGGGATAAAGGAACATGAAAACCTAAAGCCACTACTAGAACAAAAAG990.13733786502046194No Hit
GTAAAGGGGATTTAAACAAATTTTCATATGGAACTTGATAAGCATTAACCAA990.13733786502046194No Hit
CGATGCGGGAACAGCAAACTTTGTTGTGCAAATGATGAAAACATAATGTAGG990.13733786502046194No Hit
TTTTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC990.13733786502046194No Hit
AAGCTTGGGATCATTCAATATTTTTATCCTCATTATCAGTCTCATATGATCT970.13456336269681626No Hit
ACTAACGGGAGGATTCATCTCTTTGGCATTGCCAGGGTTTGAATGTGTCCCC970.13456336269681626No Hit
TAGTATGGGGGAGTATCTGGATCATTAAGTAATTTCCGGAGGTCAGAGTAAA970.13456336269681626No Hit
TTACAAGGGAGCCAGATGAAAGTAGCATATTTTTTTCAAGTACTGAAAGAAA950.13178886037317056No Hit
TAGTATCGGGTATCTTTTATCTCTCTTTTCCTTTCATCACCAGTGGCTGGTC950.13178886037317056No Hit
ACATTTGGGAATGTCAGGAAGAGAAGGTTCCGGAAGACAGCAAAGAAGAAAT950.13178886037317056No Hit
GATCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.13178886037317056No Hit
CAATCTGGGAGTGCTTGAGCTGCAGAAACTGAGACCAGACCTCTGGCCTGGC950.13178886037317056No Hit
TTTTCTGGGGCAACTTAAAATGTTTACACACTGTGGTGCAATCTACTGTTTG940.13040160921134772No Hit
GTATTAGGGATGGCTGCATTGAATGATATACGGTATCAGAGACTGGTGGTGG940.13040160921134772No Hit
TAGCCTGGGATTCCTCCAAACTCATTCTACAAGGCTAGTATTGTCTTGATAC940.13040160921134772No Hit
ATTTGAGGGATATTGGAAGTTTTGAGTGAGTGCTGTCTAGAAGGTTTTGGAG930.12901435804952488No Hit
GCGAGTGGGCTATGCTGTTGAGAACATGTGGTATGTCAGTCAGTCCTAACTT930.12901435804952488No Hit
TTTTATGGGAGTCAGTGACAGACTGTATACACATCAGGGGTCCCCCAAGATT930.12901435804952488No Hit
CATTGCGGGATGTTGAAAACAGACAGATGGCATATGAAAACCTGAACGACAA920.127627106887702No Hit
ACGACAGGGAAATTGTCAAGGCCCAAGGGCTAATTTTAATTTTCTTTTTACT920.127627106887702No Hit
TTACTCGGGATTCTTGATTATTTCTTTTTTCATCTTTTCAATCCTGCTTACT910.12623985572587917No Hit
AAGCACGGGATTTCTCAGGTGGTGGATCCAAAACTTAACCACATCTTCAGGC910.12623985572587917No Hit
TACTTGGGGAGAACTGATGCATTAAACATTCCTTAAACCATTCTAACTTAGA910.12623985572587917No Hit
CCGCTGGGTAACTATACAACCTCATCATCTTTTACAGAAACAGGTTTTGTGA910.12623985572587917No Hit
TGCCTAGGGGCTGCTTGGAGCTCTTGATTTTTAGAAATGAATAATACAAGAG910.12623985572587917No Hit
CGCTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.12623985572587917No Hit
TGGTATGGGAAGACTAAAGCTTTCATTCTAACATTCAGACATAGCAATCCAA910.12623985572587917No Hit
CATATAGGGCGATCTGGTTTAGGGGGGAAATGCTTTATAACACTGGTCTATG910.12623985572587917No Hit
TGTTGCGGGGATTCTAGTGCTTTGTAAATTACGTGGCTTAACCTAGATTTTT910.12623985572587917No Hit
TTGATGGGGATGTAAGTTTTGATGTAAGTAATGCATGTTTGACTATGAAGTA900.12485260456405632No Hit
AGAATTGGGACATGTTTCCCATGAAAATGATGCAGAAACTTCAGCAGGTAGA900.12485260456405632No Hit
ATCATAGGGAGCCATCCAGTTGATTTAGCAGGAGAATGCAGGTTTTTTTAGC900.12485260456405632No Hit
ACAGTTGGGAGCATTAATATTTTTTATTCTGGAAATCCTTTGGCAAGTATTA900.12485260456405632No Hit
TAACCGAGGAGGGGAATGAGCAAGAGCTTTGTTGTGATGGAGAAGGACTCTG890.12346535340223347No Hit
GCTATTGGCCAATAGTTATTTTTAATATTTGTATTCTTGTTATTTCTGGAAG890.12346535340223347No Hit
TGCAAAGGGGACTGGTGCAAGATGGGCCCTCCCACTGTAAACAACAAAAAAT890.12346535340223347No Hit
GTTCGCGGGTCTGAATTTTTTCATTTATTTCCTTTTTTGCCAGCAGACAGAC890.12346535340223347No Hit
CTAAAATCGGGAGTAAACTTTTCTAGAAGCTCTACGATGACTATCATAGCAG880.12207810224041063No Hit
CTGCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC880.12207810224041063No Hit
GCCATGGGGGCATATTTAGTGTAATGTCCTCAAGATTCATCCTTATTGTCAT880.12207810224041063No Hit
TCACATGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCT880.12207810224041063No Hit
TTAACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC880.12207810224041063No Hit
AGCTACGGGCTAACCACAAAGCATCAAAATAAGAGGCAAAACTGATAAAATT880.12207810224041063No Hit
CAGTAGGGGGTGTACCAACAGAAGTAGGTTGCATATTCTGTCTTTTCTTTCT880.12207810224041063No Hit
AGAAACGGGGTGGGTGAGGCCTGACCACCTTGGGACACCTGCAAGACGACTC880.12207810224041063No Hit
ATGACTGGGCAGAAGGCCCACTTGAGCCTAGGCGTTCAAGGCTGCAGTCAGC870.12069085107858778No Hit
TGGGTTGGGGTAGCAGAGCAGTCCAACCCAGATTAGTGCAGCCCGGAGGCTT860.11930359991676492No Hit
AGTCGCGGGATGCTCTACAATTTGTGCAGTTAACGCAATTATCACAGGGTCC860.11930359991676492No Hit
CAACTGGGGTCTTCCATTAAAAAACAACAACAATAACCAAACAACGACCTTT860.11930359991676492No Hit
TTATCAGGGACTTTAACAAAGAGTAGAACTATTACCAGTTTTATGTGTTGCT860.11930359991676492No Hit
ACAGCAGGATATATACTAGTTGCTATGAAAATATCATGGAATAATAGGGATT860.11930359991676492No Hit
CTTTGGGGGATAGTACAAAATTGAGTATTCCTTATCCAAAATGCTTGGGACC850.11791634875494207No Hit
AGAATGGGAATACTACTCTGAACATTTATATACATATTTTTGTGTTTTCGTA850.11791634875494207No Hit
CTTAGGGGGATGGCCACAACCCCAAATATTGGTACCATCATTGTTCTCTGTT850.11791634875494207No Hit
AATTTTGGGACGATCTTGAAATGAAACTTAGATTTTTCTGGGGAAATGTTCA850.11791634875494207No Hit
GCAATGGGAATACAAGCAGTATGGAAAACCCCGCACCAAACAAGAATCCATG850.11791634875494207No Hit
GGTAATGGGAAGAAAAATGTGAATTTGGTGGTTTTATTCTATCGGTATAAAG850.11791634875494207No Hit
AGAAATGGGAGTTGAGACCAAGAATTGGCAAATTTGCATGAGCTCCTGGAAA850.11791634875494207No Hit
TAGTCAGGGGTAACATACTTAATTTATTTTGAAGTGTTCTGCTCTTGAAGTG850.11791634875494207No Hit
TCTCAAGGGATCACTCCTGGAGGCTTTTCATCAGTGCCTGCTGGAATGGAGA850.11791634875494207No Hit
GTTAAAGGGATACATTTTTAGCAACCAGGATCCAGGTTTGCTTGGATGTGTC840.11652909759311925No Hit
ATCTGCGGGAGAAATATATTCCAGATGATAAAAACATTAAAAACATAAAGCA840.11652909759311925No Hit
TATAGTGGAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA840.11652909759311925No Hit
GAAGCCGGGATAAATAACCTAGTGATGCATCTTAAGGAACTAGAAAAGCAAG840.11652909759311925No Hit
CGACCGGGGGGGTATGGAGAAAAGGGAAACTTGAACACTGTTGGTGAAGTGT840.11652909759311925No Hit
TTTATAGGGTATGGACACCATGCAAAAATCGTCTGCCTGGCCTGGTCCCCAG840.11652909759311925No Hit
GCGCGTGGGGATCTCATTCATTTGCTGACAATAAACATGTATGACTTGTAAT840.11652909759311925No Hit
TAGCTAGGGGGGCTCTGCAGACAGCATTTGCATATGCAAGACTCAGTAGCCA840.11652909759311925No Hit
TGCAATGGGATGAGACTCGATGGCAACCCTTAAACCAGGCTGCTTTCAAATA840.11652909759311925No Hit
CGCTGTGGGGTTGAAATCTCTGGGCTAATTTGTAAGATCTATGTTTTAAAAC840.11652909759311925No Hit
ACTGTTGGGAGCTGTGTGACTCTGTATAAGCTTCTAGGCTTTCTGTGCCTCA830.1151418464312964No Hit
GCATGCGGGTGGTTAGAATGTAAATTAGTTCAGCCCCCAAGGAGAGAAATTT830.1151418464312964No Hit
GTCACCGGGGAAAAGAAAGAGCTAAATTGCCTTCCAGTTCTTTGAAGTAAAA820.11375459526947354No Hit
TTTGCCGGGGACGACGACGGCCCCGCGGGCCCGGATGCTGAGGAGGCAGGCG820.11375459526947354No Hit
CATAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA820.11375459526947354No Hit
GGGTGGGGGATGATTCTTTTGAGCTCTCAAGAGCTACTTTAGCCTTGCCATT820.11375459526947354No Hit
AGCAGCTGGGCTGTGAAAACCACCTCGTTTGCTTTTTGTTTGTTTGTTTTGC820.11375459526947354No Hit
TATAAAGGGATGCTATTCTACTTTTCAGATTTCTCGCCAAAGTGAATATGAA820.11375459526947354No Hit
TAAGCAGGGATATTTAAAATAACAGAAATCATGCAAAGTCTATTCTCAGACT820.11375459526947354No Hit
GTTTGCGGGATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATTTAT820.11375459526947354No Hit
ATTTTTGGGATGGTGTAAATAAGGGTCTAATTTAATTCTTTTGCATGTGGAT820.11375459526947354No Hit
TTCACCGGGACTGACAGAAATGTCCTCAAGAGAGGATTATATTCTTGAACCA810.11236734410765069No Hit
AAGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA810.11236734410765069No Hit
CATGTCGGGAGGGTGACAGTAGGATCTACCCGATAGTAGGGTGGCAGTAGGA810.11236734410765069No Hit
ATACTAGGGAGTAGAATTTGTGTTAGGGCACATCCCATGGAGATCTTTTTTA810.11236734410765069No Hit
CGTGAAGGGAACTTTGGATTTTATGGAAGAGTATAGTCTTATATTCATACCT810.11236734410765069No Hit
TGCACAGGGGTATCGTGGACTTATTGTATGGCATTTTAAGATCACATCTGTT810.11236734410765069No Hit
TCCTATGGGCATGAGAACATGAGGCATGAGAAGTTATTTTGATAATGTTGAG800.11098009294582784No Hit
GTCCTGGGGAAATCTCTTTCACTACAGACAAGACAGTTCAGAAGTGAAAAGT800.11098009294582784No Hit
AAAACCGGGCTTTCCAAAATATTAGCAAATGGAATTAAGCAATTTATAAAAA800.11098009294582784No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC800.11098009294582784No Hit
GATTGGGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCT800.11098009294582784No Hit
ACAATAGGGAGAGCGCTGATTGGTGATTTTTAGAATGCTCTTGCTGTCTGCA800.11098009294582784No Hit
CGCATTGGGAGACACGAAAACCAGAACTGCATCAGGATATGGTGGTACCAGG800.11098009294582784No Hit
TGTGGGGGAGGCTGCACTTGATTATATTTGATTCTTTGTTCGTAATACATGG800.11098009294582784No Hit
ACATACGGGGGGTGCCAGCCAGCTGCTGGTGATCCTCAGCCGGAGGGCAGGG800.11098009294582784No Hit
AATATGGGCTAAGGAGGAGCCGCCAGTGCCTGAAGCCCCCAGCTCGGGGCCC790.109592841784005No Hit
TAAATAGGGGACAAAAATAGATTGTGCAATTAAATTGCGTATGCAATAAACT790.109592841784005No Hit
TCAACAGGGGGAATTCATTACAATTTAACTTTGAAAAATAGAAATAATACTT790.109592841784005No Hit
TGTTTAGGGAAGAACATTTTTGAGTGACAATGCTTCAGTAAAACTGACCTTC790.109592841784005No Hit
GACTGGGGTGGCAAGATTACAGATATTTTCTTCAATTATTTCCTCAAATATG790.109592841784005No Hit
GTTTAAGGGTAACAGGATCTACAGGATGATCAAACTTGGTCTGGGTATTGAT790.109592841784005No Hit
TATGAAGGGAAGGAATGAAGGGCAGAGAAAACAACATGTGCATCACAAACTT790.109592841784005No Hit
CTTCATGGGAGCTTACAGTTTTCCACAAGGACTTTAATAACTAGCTGGGAAA780.10820559062218214No Hit
CGGCTGGGTTTCAACCTTGATTTTCAGTTTTTATATGATGGGAAATATAGTT780.10820559062218214No Hit
TGCAATGGGGCCAACTTCCAGCGAAACCAAAAGCACATGAACTAAGTAATTT780.10820559062218214No Hit
AGAAGTGGGCAGCCCTTCCCTTCTGTATTTGGGGCCTATTTATGCCACCAGT770.10681833946035929No Hit
AACGATAGGGATGAATTTGGGAGGGACACAGACATGGAGTCTATAACAGGAG770.10681833946035929No Hit
TTTTTGGGGACACAGTGGGATGTTAAAGGTTGAATGTCTTTGGTATCTATAA770.10681833946035929No Hit
GTACTAGGGTAATGGAACAGCATCATTAAAGTGCTGGAAAAATATCAACATA770.10681833946035929No Hit
TAAATAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG760.10543108829853644No Hit
TTGAATGGGAAGATGAAGGTGACAACAAAAGTCAACCGTCATGACAAAATCA760.10543108829853644No Hit
ACTAAAGGGTTACTGTTCTAATATAATTGAATGTTAGAAAGAAAATTTACTT760.10543108829853644No Hit
CTTGTAGGGAACATCTGGCCCAGCAGGCCCAGACTGTATCCATCCAAGTTCC760.10543108829853644No Hit
GACACTGGGATAGTTGTTTTATCCATTATCATCTTTGATTAAGACTTTAAAA760.10543108829853644No Hit
AACGCTGGGGGCGGCGGGAAATTGTTTCCCAGAGCCCGGATTCGTGAAGCAG760.10543108829853644No Hit
CAGCATGGGAGATTAAATGAATACAGATAAAATAATAAGCACAGTGTCTGAC760.10543108829853644No Hit
TCTAAGGGGATTCCTCAGAGTTAATTGACTCAAGGAAATAATTTGAAATTTC760.10543108829853644No Hit
GATCTAGGGGCTCTTCCGATCTGCATGTGGGACACTTTGCTAATATTTTAAT760.10543108829853644No Hit
TATTACGGGAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCT750.10404383713671361No Hit
AGCTTGGGGATGTCTGGCAACTCAAAATGTAATGGATTCATCAAGCTATTTG750.10404383713671361No Hit
GTTATGGGGATATTGCCAAAAAAAATCAAAGCATTACTGACCTTAAACAGCT750.10404383713671361No Hit
TACCACGGGGTGGGCAGTCATGAAAATCAATTCAGACTGTGTTGATTAGCAG750.10404383713671361No Hit
TGTAATAGGGTGGTAACTTTGTAGAAGGTTATTGTTATGAATGCTGTGGGAG740.10265658597489076No Hit
GCAGTCGGGTCACGCGTCACTGCAACCTCTGCCTCCCGGATTCAAGCAATTC740.10265658597489076No Hit
GTAACCGGGAGTACAACTTTGATACCAGGAATGCAGTAAAACTTTGACAGTG740.10265658597489076No Hit
TGTAGTGGGGAATAGTGCAACCTTAGAGCCTTATGGCTACAGAAAACTAAAT740.10265658597489076No Hit
CGGGTGGGGGAAACACACCTAAACACTTTATCTCCAAGTTACAAAAGTTTGA740.10265658597489076No Hit
TTGAGAGGGGAAGGTCAAAGAAAAAGTTCAAAAACTGAAGGACTCTGAAGCT740.10265658597489076No Hit
TATTTAGGGATTTGATTAGGATTAACAAACAAGAATATGAATAAACCAATAG740.10265658597489076No Hit
TAATAAGGGAGATCCATTCAGTGGCTCATGTGCAGCTTTCATAAAATAAAAT740.10265658597489076No Hit
CATTTCGGGGACCCAGAGGTTGGGGAACCCTTTTTTAAGACATCAACTTTTC740.10265658597489076No Hit
ATTTGCGGGGTATGTATTACCTGTCAAAAGTGTATCACCTGAGGTGAAGTTT740.10265658597489076No Hit
CTCAGCCGGGATTTCATAAAGGTTCAATTACTCCATTTCCTTTAACTCTCCA740.10265658597489076No Hit
GCATCTGGGGGTTTGTGCCTTGTTCTGAATTATTTTGGGGATTATGACAAGG740.10265658597489076No Hit
AGTTCCGGGACAAAGGCATGTTTTAAAATCTATAACATAAAGAATAAGGAAT730.10126933481306791No Hit
AATTGTGGGACTTGAAAATGGGGGTATAAAGGGGTATATATTTTTTCCAGAA730.10126933481306791No Hit
GATGTGGGGTCCGATCTTCTCGTGGGTCTTCCGATCTGGGCATGGGCAAACC730.10126933481306791No Hit
TTCTCGGGCAAGGGAAGTGCAAGGGCTCAGGATGAAAGAAAATCTATTGGGA730.10126933481306791No Hit
TACGCAAGGGGCGATGACCTTGAGTAGCAGGATATAAAAAACCCCACAAGCT730.10126933481306791No Hit
CCAAAGGGGTCTTCCTTTTTGAAAAGTACTCTCATAGAAGAAATTTAGCAAT730.10126933481306791No Hit
GACATGGGGACTTTGTATCTTCACTGCCAAGCAAAAAGTCTGCCAAGTGGGA730.10126933481306791No Hit
TCATTTGGGATGAATGTAATTACCAAAAGATTTAGAAGTGCATGTGCTTTGG730.10126933481306791No Hit
AATTAAGGGATGCCAGGTTGTGTGCAAGCCACTGTCACGTTCTTTATGTTAT730.10126933481306791No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)