FastQCFastQC Report
Sat 31 Dec 2022
SRR5848716.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848716.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145796
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT7040.4828664709594228RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC6270.4300529506982359No Hit
TTTGTGGGTAAAAGGTTAGAGGAATTGCTAACTAGAATAACCAGTTTAGAGA1570.10768471014293945No Hit
CAGTGGGGCAAGTAATTCTCCTCCTCAGCCTCCTGAGTAGCTGGGGCTACAG1530.10494115064885182No Hit
TCCGTGGGGATTTTGGATAAGGGATACTCAACCTGTAATATGTTGTGAAATC1470.10082581140772037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)