FastQCFastQC Report
Sat 31 Dec 2022
SRR5848708.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848708.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88772
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC3650.41116568287297794No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT3010.33907087820483933RNA PCR Primer, Index 40 (95% over 22bp)
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1840.2072725634208985No Hit
CACTAGGGGGGAAAAGGATCAATTTAAGTGGTCATTTTATAGTAAGAGATGG1330.14982201595097555No Hit
TGCTTGGGGGACCTTCTCCTTCACCTTTGTCCCCACCGCAGACAGGACCAGG1320.14869553462803586No Hit
GTAACGGGGGAGTTTATGGGGGAAATAGGTAATACAAACATTTGACAATGAA1310.1475690533050962No Hit
ATTTGAGGGCAGGCAGTGGTCCCAAAAGAAAGAAGGAGGAAACAAAACACCT1250.14081016536745822No Hit
GATCACGGGTACACTGTTGGTGGGACTGTAAACTAGTTCAACAATTGTGGAA1240.13968368404451856No Hit
ATCGACGGGAGGTGAATGCAGTGGTTCTCCTTCATGCAACAGCAGGGGCAAG1220.1374307213986392No Hit
GTAACCGGGAACTTCTAAAACTTCCAAAAATGACTTCCAAAAACTTCTTCCA1220.1374307213986392No Hit
GTTGCAGGGGAAAGGCCAAAATATTTTTATATTGCCACAGACAAAGCCACCT1190.1340512774298202No Hit
TGCACTGGGATTGACTGCTGACAACGTAACAGGGAGCCTTCGGGTATTTGAA1190.1340512774298202No Hit
GCAGTTGGGCGCATTGAAAACGATGTTGCCACCATCCTGTCTCGCCGTATTG1190.1340512774298202No Hit
AATATTGGGACCAGAGGCTCAGTGCATTGCTAAGATGGTGAATTGCTTTTTT1180.13292479610688054No Hit
TGTCATGGGTGTGGCTCTGAAGTTTGTTTTTTAAATGACCACAAGTGTAAGA1150.12954535213806156No Hit
GTAGTTGGGAGATCATGTACTGATGAACAAATAAAGATAGCCTTTAGTTTCA1150.12954535213806156No Hit
CGCAGTGGGGAACATATCCGGGAATAATTGAAAAAACCAGGCAATCAAATGT1140.12841887081512188No Hit
AGCCTGGGCCGATCTTTAGCGGGGGACTTGCTCAGTAAAATCCTGACTGTTG1130.12729238949218222No Hit
CTGGCTGGGACAGCAGACAATCAACAAATATGTGTGGCAAAAACTGCTAGAA1130.12729238949218222No Hit
TGATTTGGGATAATTGCAAAGTTACAGGGATACTATTTCACATGGATTAAAA1120.12616590816924256No Hit
TAGCCGGGGCCGATCTGCCAAGGGATTATTTAAAACTTTTAGCTAGAGTTCT1120.12616590816924256No Hit
AAGCATGGGAGCTGTGTTTGAGGAAATTCACCTAAGAGACTGTCATCTGAGC1110.1250394268463029No Hit
TTCGGGGAGTCATTAATTTTCACAATTCTACAATGGCTTTAGAAAGAGGAAA1100.12391294552336324No Hit
AGCAACGGGATTAATGAACTAGGTATCCAACATAACAAGATAAACATAAAAA1090.12278646420042355No Hit
TGGCTAGGGCTGTGTTTCTAAGTCAATTCTGTAAACCTATGACACTCCTCGA1090.12278646420042355No Hit
GAATTTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1080.12165998287748389No Hit
ATGATAGGGAATCCAAATCTATTTTTCTCACGTGTTTAAAAAACTGAAAAGT1080.12165998287748389No Hit
ATCACGGGAGCAAGAGATGCTTTATTCTGCAGCATGAACAGATTTAAAATGG1070.12053350155454422No Hit
CGGCTTGGGTACATTAATATATGATCTTCCATGACGAGGTCTGAAATGAACA1060.11940702023160456No Hit
TCTCGAGGGCAGCGGGTCAGGCCTCTGCCCAGCGCCTGTGGTGGGACCCTGT1060.11940702023160456No Hit
TGTATGGGGAACATTGTTTTTCCAATATATTAGTATTACAAGTGCCTAAAAC1060.11940702023160456No Hit
TGCTGTGGGATAAGAGAACACCAGATAATATGCTTTATTTCTTTCGATCTTT1060.11940702023160456No Hit
CTCGCAGGGAGCTGCCTTCCAAGCAGTTCCTCTGGAATTTCCAAGAATAATC1050.11828053890866488No Hit
ATAGCCGGGCCACTCTCTGGATCCAGTACATCTGAGTCACTGGAAAGTAGCT1050.11828053890866488No Hit
TTAAGCGGGAATTCCTTCCACGACCCCTGAAGAGCAGCGGTGGGTCTGAGCA1050.11828053890866488No Hit
CTATTCGGGGGATTATTATATTATGACAATGAGTTCATGGGAATGTGGAAAT1050.11828053890866488No Hit
CGCTTCGGGATGAAAATATATTGGATTATTTTTGAGAGAATGAATCTTTACA1040.11715405758572522No Hit
TCATAGGGGGGCACTCTGCGCCCGGAGGACAGAGCGGCCCGGTCGCCGGCAT1040.11715405758572522No Hit
CTGACAAAACACATAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAA1040.11715405758572522No Hit
CACAATGAGATACCATCTCACACCAGTTAGAATGGCAATCCTTAAAAAGTCA1040.11715405758572522No Hit
GGCCATGGGATGGTGCTGGAAGTTCCAACCACTGAAATAAGGTAAGAAAAAG1040.11715405758572522No Hit
AGATGCGGGACTGCTTTTACCTACACAAATGATTTAAAGGAAAGAACACAGC1030.11602757626278558No Hit
TGAACCAGGGTGGGAATGCATAAATTATACAATAAAAATTGGCTTAGTAAGC1020.11490109493984589No Hit
CACCTTGGGGCGGCGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGA1020.11490109493984589No Hit
TCACCCGGGGTCTCTTAAAGTTTCTTTCAGCTTTTAAAAATCCCCTCATTAT1020.11490109493984589No Hit
GAACTCGGGTGACGTTCTCATAGTTGATTCGGATGAAGAAGATTCTTCAAAT1020.11490109493984589No Hit
TACGATGGGACAAGTCAGAATGTGAAATTAAATAATGGTTTGAACAGAAAAT1010.11377461361690624No Hit
TGTGACGGGTCTCTGAAGGGGATTATGTCAAAGGAATATATTTTATAAAAGC1010.11377461361690624No Hit
CACATTGGGATCTTCAGAGTTTTCCTAGTTCTTCTAGAAATACATGTCAGGT1010.11377461361690624No Hit
ACCATCACCCATGAGGGATCAAAGAATGCAGCTGGCTTAGAGCAGGCTGGCT1010.11377461361690624No Hit
TATGCGGGATGTACAAATGTGGCTCTAACAAATCTCCAGCAACAAGAAAAGA1010.11377461361690624No Hit
AGCCACGGGACTGCCCTGTTTACAATAAGTATACGTGAATAATCTATTATTA1000.11264813229396656No Hit
GATATAGGGGAAAGTATTTACAGCCTGCTGTGTGTATCTTGTGTCATTTATT1000.11264813229396656No Hit
ACGTACGGGATTGCTAAATATGTTTCTTTCATACGTTGCAGTGCTTACAGTT990.1115216509710269No Hit
TTCATAGGGATTTCAGTACAGACAGCCTGTTATTTAACATAGACTCACAACA990.1115216509710269No Hit
AAAGGGGGAGGTCGGGCTTGGGAAGGGTCAGCGGGAGGCCTGAGGGCGCCGG980.11039516964808722No Hit
AATTACGGGGCACTAAGCATTGTCTGCCAGCTTCGTATGGATAGAGGTGGAA980.11039516964808722No Hit
ACGCGCGGGTCTTGCTGGAGGGCTACAAGTCAGTGCCCTGTATGCTCTCCAG980.11039516964808722No Hit
TCAAGGAGGGATTTAATTTGCAAATAAAGGTGGTTGGAAAATTGTACTTTTG980.11039516964808722No Hit
CAGACTGGGAGCGGAGGGGTTTGGGAGTCAGGCCTGGGCAGGACCCTGCTGA980.11039516964808722No Hit
CACGAAGGGCTTTGGAGAGATATCTATTCAAGTCCTTTGCCCATTTTTAAAT970.10926868832514756No Hit
AGGTGAGGGTGATCATGTTCTCCTGAAGTGCGCTTGGTTTGTGACAAGTGTA970.10926868832514756No Hit
GAATCCGGGGCTGGGGTTCACCCTGTCCCAGAGCTCTGGCTAGGGTGCTGGG970.10926868832514756No Hit
ACACACGGTAAGACAAAAGGTGGAGGTATTAAGAATGGTAGCTTATGTATAC960.10814220700220792No Hit
TTCGTGGGGGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAACTACTTAA960.10814220700220792No Hit
GTGATGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA960.10814220700220792No Hit
AACCATGGGTATGGAAACAAGAAATACAGAAAATAAGTCAATCTGAAAGATT960.10814220700220792No Hit
TGTTGCAGGGACTGTGAATATTGCTGGGCTGCTATCCATTCTCGTGCTGGCA960.10814220700220792No Hit
ATTGCTGGGATGCCTTAACTGACATTGTTTTGACACATATTTAAAATACACA960.10814220700220792No Hit
CTCTGCACCCCAGCCCTGTTTCACCTTCAGCCATCCCAACAGAGATCCTATG960.10814220700220792No Hit
TTTTAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG950.10701572567926823No Hit
TTTTATGGGATTGAAGCAATACGCTCTGGGGGAAAAATAACTGTTTGACTTT950.10701572567926823No Hit
TTAAATGGGCAAAAACAGATTTCCTAGCCTTGGCTGATAGCCCATTTACTCC950.10701572567926823No Hit
TAAACAGGGAGGAGAAGGTTCTGAATAGACATTTCTCCAAAGACAACATCCA950.10701572567926823No Hit
CCGATAGGGAAATACCACAGGGAAGCCATGATGATTCAATTATTTTTTTCAG950.10701572567926823No Hit
AGATGTGGGATCTCTTGTTAAGGATTTTGCATATATTAGTAAGTTATTTTTA950.10701572567926823No Hit
ACTACTGGTGAACTACTCAAGATAGTCACGAAAATACTGAAAGTTTGATTTT940.10588924435632859No Hit
CGTCGCGGGAGGGACTGAAGGTCACACCACAGCGCGTGAGTTTCGCTCTGTT940.10588924435632859No Hit
TCGGCGGGTATGAAGCCAGTATCACCCTAATATCCAAACCAGGAAAGCACAC930.1047627630333889No Hit
ACCTGGGGGACTACAGGTATTATGTAGCCAGCTGTCTGCTTTTGTAAATAAA930.1047627630333889No Hit
GCGCATGGGGTAATGGCCAAACTAGAATCCTCTGAGGAAAGCAAAAACCTAG930.1047627630333889No Hit
AAACACGGGCTTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGC930.1047627630333889No Hit
AGTAATGGGGCATACGGGGTCTTGTTAAAGAGTATACCAGTGCTCTAAGAAA930.1047627630333889No Hit
GTGCCTGGGGTAAGAAGCTATTTTATAGCTTGCCAGGTTTACAAACTCAGAC930.1047627630333889No Hit
CGCTAAGGGAGCCTACCCCACAGGCAACATTCAATTCCTGTCTGAATTGAGA930.1047627630333889No Hit
TATTTTGGGTGGTTGCTTAATTCCCAACACTCAATTTCCTTCTTCTAAACAC930.1047627630333889No Hit
GGATTAGGGGAACTCTTTGACATCTAAAGATCCAGATATCAAGGCACAATAT930.1047627630333889No Hit
TAGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.10363628171044925No Hit
ATTCAAGGGCTTTTGCAAAGTTGTATCTACATTTTACACAAAGATAAAGAAT920.10363628171044925No Hit
GCCGCAGGGGACCCATCAGAGTATTATCAATATGAAGGAAATAGTGACTGAT910.10250980038750956No Hit
AAGTCAGGGGCATTAGGAGCCATTTGAAATTGACAGTCAAGTGGGCAGTAAC910.10250980038750956No Hit
AACAAGGGGGTGGACCCGCTGGTGCGGGATGTGGCAGGAACTCTGGCTGAAA910.10250980038750956No Hit
GACTCCGGGAGTCACTGTACTTTCTGAGTCTCAGTTTCCCCATTTGCAGAAT910.10250980038750956No Hit
TTTAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.10250980038750956No Hit
ACGTCGGGGTCAGTTAACTCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGC910.10250980038750956No Hit
TTGGTACGGGATCATCAAGAGACTCAAGAAGAGGAAGGAGCAAACCAGAAGG900.1013833190645699No Hit
TTTGCTGGGTTCAAGAATATGGGTGGGATATGGAATGCTCTTTCAATGTCTA900.1013833190645699No Hit
ACACAGGGGATCTTTTTGGGGGAGGTGCAAACGTCCTCAGCAAAATATAGCA900.1013833190645699No Hit
CTGTTCGGGGTGCCAACAAGTTGATATTACTCAAAAACAGCTGGAAGAAGAA900.1013833190645699No Hit
GTCGACGGGGAAAGAAATTTCGTAAATGTTTGAAGGGTGAGTTTCATCAGCT900.1013833190645699No Hit
GCCTGGGGAGCACTGCAAAGTTTCCTAATCAGCAGCCCAGCTTTCCCTCCTA900.1013833190645699No Hit
CACAGGGGGGTCTTCTAGAAGCATAAGAAATAATATCATACGTCAAATATTA900.1013833190645699No Hit
CTTATCGGGTTAGGGGTCAGTCCTTGAATTGAGACCCATGTTATCTTTGGGA890.10025683774163026No Hit
ATTTAGGGAGTAGCATTTCAGTGTACTTTCTTGAGAGGGGAAAACAGAGTTT890.10025683774163026No Hit
CGTAGAGGGTAGTCATTATGGTTAATAAACACTTACCATTAGGAGGAAGAGG890.10025683774163026No Hit
AAGTTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.10025683774163026No Hit
TTCACAGGGGAAGCGGAGGGAGTCGAGCCAGCGTCGCCGCGATGGTGTTGTT890.10025683774163026No Hit
CTGAAAGGGGACAAACCAAACCAATTTATTAACATCATTATCATTAGTAGTA890.10025683774163026No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)