FastQCFastQC Report
Sat 31 Dec 2022
SRR5848707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88126
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC3530.40056283049270364No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT3280.37219435807820617RNA PCR Primer, Index 40 (95% over 22bp)
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1690.1917708735220026No Hit
TGCTTGGGGGACCTTCTCCTTCACCTTTGTCCCCACCGCAGACAGGACCAGG1350.15318975103828608No Hit
GTGTCTGGGATAGCTTGCCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGT1250.14184236207248713No Hit
CTCGCAGGGAGCTGCCTTCCAAGCAGTTCCTCTGGAATTTCCAAGAATAATC1230.13957288427932732No Hit
CACTAGGGGGGAAAAGGATCAATTTAAGTGGTCATTTTATAGTAAGAGATGG1220.13843814538274743No Hit
AAGTCAGGGGCATTAGGAGCCATTTGAAATTGACAGTCAAGTGGGCAGTAAC1220.13843814538274743No Hit
TGCACTGGGATTGACTGCTGACAACGTAACAGGGAGCCTTCGGGTATTTGAA1210.1373034064861675No Hit
GCAGTTGGGCGCATTGAAAACGATGTTGCCACCATCCTGTCTCGCCGTATTG1200.13616866758958762No Hit
GTAACCGGGAACTTCTAAAACTTCCAAAAATGACTTCCAAAAACTTCTTCCA1180.13389918979642784No Hit
GTAACGGGGGAGTTTATGGGGGAAATAGGTAATACAAACATTTGACAATGAA1160.13162971200326806No Hit
ATCGACGGGAGGTGAATGCAGTGGTTCTCCTTCATGCAACAGCAGGGGCAAG1150.13049497310668814No Hit
GTTAAAGGGATTGTTACTGGGGTGTATCATGACTTTAAGGAAAATTATCAAA1140.12936023421010825No Hit
ATTTGAGGGCAGGCAGTGGTCCCAAAAGAAAGAAGGAGGAAACAAAACACCT1140.12936023421010825No Hit
ACGCGCGGGTCTTGCTGGAGGGCTACAAGTCAGTGCCCTGTATGCTCTCCAG1130.12822549531352836No Hit
AGGTGAGGGTGATCATGTTCTCCTGAAGTGCGCTTGGTTTGTGACAAGTGTA1130.12822549531352836No Hit
CGCAGTGGGGAACATATCCGGGAATAATTGAAAAAACCAGGCAATCAAATGT1130.12822549531352836No Hit
CTGACAAAACACATAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAA1120.12709075641694845No Hit
CTATTCGGGGGATTATTATATTATGACAATGAGTTCATGGGAATGTGGAAAT1110.12595601752036856No Hit
TATTCTGGGCAATGCATTTTGAAAATGATTTGTGAGCTTTATTTGAAGTCAC1100.12482127862378867No Hit
TGTCATGGGTGTGGCTCTGAAGTTTGTTTTTTAAATGACCACAAGTGTAAGA1100.12482127862378867No Hit
GAACTCGGGTGACGTTCTCATAGTTGATTCGGATGAAGAAGATTCTTCAAAT1100.12482127862378867No Hit
GACTCCGGGAGTCACTGTACTTTCTGAGTCTCAGTTTCCCCATTTGCAGAAT1080.12255180083062887No Hit
TTTGCTGGGTTCAAGAATATGGGTGGGATATGGAATGCTCTTTCAATGTCTA1070.12141706193404898No Hit
TTCGGGGAGTCATTAATTTTCACAATTCTACAATGGCTTTAGAAAGAGGAAA1070.12141706193404898No Hit
GCCAATGGGGTCAGAACCTATAACATAGATCTGAAGTCAGGCTCGAAATCAT1070.12141706193404898No Hit
TGATTTGGGATAATTGCAAAGTTACAGGGATACTATTTCACATGGATTAAAA1070.12141706193404898No Hit
AAGCATGGGAGCTGTGTTTGAGGAAATTCACCTAAGAGACTGTCATCTGAGC1070.12141706193404898No Hit
TCAAGGAGGGATTTAATTTGCAAATAAAGGTGGTTGGAAAATTGTACTTTTG1070.12141706193404898No Hit
TGATCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1070.12141706193404898No Hit
AATATTGGGACCAGAGGCTCAGTGCATTGCTAAGATGGTGAATTGCTTTTTT1060.12028232303746907No Hit
GTGCCTGGGGTAAGAAGCTATTTTATAGCTTGCCAGGTTTACAAACTCAGAC1060.12028232303746907No Hit
TTGGTACGGGATCATCAAGAGACTCAAGAAGAGGAAGGAGCAAACCAGAAGG1050.11914758414088918No Hit
TACACAGGGGTTCTTGCCCGGTATTTCACTCGTTTGTCTCTCCTCATGACAC1040.11801284524430929No Hit
ACCGGAGGGATTGTCTACAGACTGCATTATTTTCCCTCGTTAGTTAAGTTTC1040.11801284524430929No Hit
AAAGGGGGAGGTCGGGCTTGGGAAGGGTCAGCGGGAGGCCTGAGGGCGCCGG1030.1168781063477294No Hit
AGCAACGGGATTAATGAACTAGGTATCCAACATAACAAGATAAACATAAAAA1030.1168781063477294No Hit
CTCTGCACCCCAGCCCTGTTTCACCTTCAGCCATCCCAACAGAGATCCTATG1030.1168781063477294No Hit
TCGGCGGGTATGAAGCCAGTATCACCCTAATATCCAAACCAGGAAAGCACAC1020.11574336745114949No Hit
GGCACCGGGGGACCACCATGGTAAAGAAGCGGAAAGGCCGCGTCGTGATCGA1020.11574336745114949No Hit
GGCCATGGGATGGTGCTGGAAGTTCCAACCACTGAAATAAGGTAAGAAAAAG1020.11574336745114949No Hit
CTGGCTGGGACAGCAGACAATCAACAAATATGTGTGGCAAAAACTGCTAGAA1020.11574336745114949No Hit
CTTATCGGGTTAGGGGTCAGTCCTTGAATTGAGACCCATGTTATCTTTGGGA1010.1146086285545696No Hit
TATGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.1146086285545696No Hit
AATTACGGGGCACTAAGCATTGTCTGCCAGCTTCGTATGGATAGAGGTGGAA1000.11347388965798971No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC990.1123391507614098No Hit
AGTGCAGGGAACAACGTGTATGCAAATTGTTATGGATACATTTCAGAATCTA990.1123391507614098No Hit
CACGAAGGGCTTTGGAGAGATATCTATTCAAGTCCTTTGCCCATTTTTAAAT990.1123391507614098No Hit
CACATAGGGTGGCCCGGCTGCAGTGGATGTTGCTAGAACCCACGCGGAGGAA980.1112044118648299No Hit
TTTAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC980.1112044118648299No Hit
AGCCTGGGCCGATCTTTAGCGGGGGACTTGCTCAGTAAAATCCTGACTGTTG980.1112044118648299No Hit
ATGATAGGGAATCCAAATCTATTTTTCTCACGTGTTTAAAAAACTGAAAAGT980.1112044118648299No Hit
TAGTCCGGGAGCGACTTCCGTATACATAAAGGGACATATTAATACTGGTTCA980.1112044118648299No Hit
ATAGCCGGGCCACTCTCTGGATCCAGTACATCTGAGTCACTGGAAAGTAGCT970.11006967296825002No Hit
ATTTTGGGGACAACTCAAACTCCTCAGACACACTCTGATAAATAGACATACC970.11006967296825002No Hit
TGGCTAGGGCTGTGTTTCTAAGTCAATTCTGTAAACCTATGACACTCCTCGA970.11006967296825002No Hit
ACGAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.1089349340716701No Hit
AACAAGGGGGTGGACCCGCTGGTGCGGGATGTGGCAGGAACTCTGGCTGAAA960.1089349340716701No Hit
GTAGTTGGGAGATCATGTACTGATGAACAAATAAAGATAGCCTTTAGTTTCA960.1089349340716701No Hit
AGTGCTGGGATTTTTTTAAATTTTTAGAAAGATTTTCTGAATATTAGCAAAT960.1089349340716701No Hit
GCCTATGGGGAGTGGCCCCACCTGTGTCCCCGATGCTGCCGTCACCTGGTCC960.1089349340716701No Hit
AGCCACGGGACTGCCCTGTTTACAATAAGTATACGTGAATAATCTATTATTA960.1089349340716701No Hit
ACTACTGGTGAACTACTCAAGATAGTCACGAAAATACTGAAAGTTTGATTTT950.10780019517509021No Hit
ATCACGGGAGCAAGAGATGCTTTATTCTGCAGCATGAACAGATTTAAAATGG950.10780019517509021No Hit
AATACAGGGCTTGATTTGTTCCTTGGCTCAAAAGTAGGGACAAAAAAGAAAA950.10780019517509021No Hit
ACCTCTGGGATGTCAAATATGAGGCCCCAGGATTCCTGGCGAGGTCCTCCTC950.10780019517509021No Hit
CACAATGAGATACCATCTCACACCAGTTAGAATGGCAATCCTTAAAAAGTCA950.10780019517509021No Hit
GGTACTGGGACTCCATTGTATTGCCTTTGGTCTTCTGTCATGGATCAGTTTA950.10780019517509021No Hit
CATATAGGGAAGAATTAAGCTGTTAGTTTTTCCCTAATTATCAAATAAAAAA950.10780019517509021No Hit
TTTTAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG940.10666545627851032No Hit
ATACCAGGGATTTCATTTGTGAATGGAATTTTAAGCAACAGTAAAGGATTCT940.10666545627851032No Hit
TACGATGGGACAAGTCAGAATGTGAAATTAAATAATGGTTTGAACAGAAAAT940.10666545627851032No Hit
CTCGTTCAGGGATAGTATATGTCATTTCATTATGTATACATTTCAATATGTA940.10666545627851032No Hit
CGGCTTGGGTACATTAATATATGATCTTCCATGACGAGGTCTGAAATGAACA940.10666545627851032No Hit
TAAACAGGGAGGAGAAGGTTCTGAATAGACATTTCTCCAAAGACAACATCCA930.10553071738193041No Hit
TAGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA930.10553071738193041No Hit
GATCACGGGTACACTGTTGGTGGGACTGTAAACTAGTTCAACAATTGTGGAA930.10553071738193041No Hit
AACCATGGGTATGGAAACAAGAAATACAGAAAATAAGTCAATCTGAAAGATT930.10553071738193041No Hit
TCTTCGCGGGGAAACTGAGGTGCAGAAATGCCATATGTTGACTAAGGATAGG930.10553071738193041No Hit
ATTTAGGGAGTAGCATTTCAGTGTACTTTCTTGAGAGGGGAAAACAGAGTTT920.10439597848535052No Hit
GGGTCCGGGTTTATTATTAAAATATAAATATTCTATTAGCAAAAATATATTC920.10439597848535052No Hit
TGTGACGGGTCTCTGAAGGGGATTATGTCAAAGGAATATATTTTATAAAAGC920.10439597848535052No Hit
TTCGTGGGGGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAACTACTTAA920.10439597848535052No Hit
GATATAGGGGAAAGTATTTACAGCCTGCTGTGTGTATCTTGTGTCATTTATT920.10439597848535052No Hit
CCGATAGGGAAATACCACAGGGAAGCCATGATGATTCAATTATTTTTTTCAG920.10439597848535052No Hit
TCACACGGGATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCC920.10439597848535052No Hit
GTTGCAGGGGAAAGGCCAAAATATTTTTATATTGCCACAGACAAAGCCACCT910.10326123958877063No Hit
GTTTCCGGGGAACTGCCTTCCCATTTTCCTATCTAACTAAAAGACAGGTGGT910.10326123958877063No Hit
TTGTAGGGGATCTCTGAGTCTGTTAAGTCAGAATATCTGGGGTTGGAACCAG910.10326123958877063No Hit
CAGCTTCGGGATCTGATATAATTGAGAAATGTGTCCTTAGACTATGTTCAAA910.10326123958877063No Hit
GTCGACGGGGAAAGAAATTTCGTAAATGTTTGAAGGGTGAGTTTCATCAGCT910.10326123958877063No Hit
GCAAAAGGGCAGATGTCCTGGAGCAACAGCTCTGAAAAATGTCCAGAAATAG910.10326123958877063No Hit
CAGACTGGGAGCGGAGGGGTTTGGGAGTCAGGCCTGGGCAGGACCCTGCTGA910.10326123958877063No Hit
GGCGTAGGGCTAGTCAATATACAACTATTAGGTGCTGTGCCCCAAAATATCT900.10212650069219072No Hit
GCGCATGGGGTAATGGCCAAACTAGAATCCTCTGAGGAAAGCAAAAACCTAG900.10212650069219072No Hit
GAGCAAGGGGACTTTAGGTTTTTGTGTGTTATTTTCTATTTCTGGGATGTTT900.10212650069219072No Hit
TTTGTCGGGACCTTGCAACTTTGTTGCTAGCTTGAGGTTGATTATTGTGGTT900.10212650069219072No Hit
GCGCATGGGGGAGGAAACATACATGATACCATGACCATGAAAAGTTTTAGTA900.10212650069219072No Hit
GACACTGGGACATACCTCAGGTCTATTTTACCTTACACTGAATGTTCTTAAT900.10212650069219072No Hit
TGGTTTGGGGTATCCTGTGAAGCAATGTGACCAATACAAAGATGAAGAAAAC900.10212650069219072No Hit
TATCAAGGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTA900.10212650069219072No Hit
CTTAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA890.10099176179561083No Hit
TTTTATGGGATTGAAGCAATACGCTCTGGGGGAAAAATAACTGTTTGACTTT890.10099176179561083No Hit
TGAACCAGGGTGGGAATGCATAAATTATACAATAAAAATTGGCTTAGTAAGC890.10099176179561083No Hit
GCGATGGGAGACTGCTGTGCTAGCAATGAGTGAGGCTCTGTGGGTGTAGGAC890.10099176179561083No Hit
GACACAGGGGGGGAAGAGACTCTAAGTCAGACTAGCTGCTTCAGTCCTGGCA890.10099176179561083No Hit
CTGTTCGGGGTGCCAACAAGTTGATATTACTCAAAAACAGCTGGAAGAAGAA890.10099176179561083No Hit
TATTTTGGGTGGTTGCTTAATTCCCAACACTCAATTTCCTTCTTCTAAACAC890.10099176179561083No Hit
GATGCTGGGCGGCAGTGGTTCCATAAGATTATAATACTGTATTTTTACTATA890.10099176179561083No Hit
ACGTATGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGGC890.10099176179561083No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)