FastQCFastQC Report
Sat 31 Dec 2022
SRR5848706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89222
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT3130.35081033825738045RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC3060.34296473963820584No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1740.19501916567662683No Hit
CACTAGGGGGGAAAAGGATCAATTTAAGTGGTCATTTTATAGTAAGAGATGG1380.15467037277801438No Hit
GTAGTTGGGAGATCATGTACTGATGAACAAATAAAGATAGCCTTTAGTTTCA1360.15242877317253592No Hit
TGCACTGGGATTGACTGCTGACAACGTAACAGGGAGCCTTCGGGTATTTGAA1350.15130797336979668No Hit
CTGAAAGGGGACAAACCAAACCAATTTATTAACATCATTATCATTAGTAGTA1270.1423415749478828No Hit
CACAATGAGATACCATCTCACACCAGTTAGAATGGCAATCCTTAAAAAGTCA1260.14122077514514358No Hit
ATGATAGGGAATCCAAATCTATTTTTCTCACGTGTTTAAAAAACTGAAAAGT1250.14009997534240434No Hit
CACGAAGGGCTTTGGAGAGATATCTATTCAAGTCCTTTGCCCATTTTTAAAT1240.1389791755396651No Hit
TGATTTGGGATAATTGCAAAGTTACAGGGATACTATTTCACATGGATTAAAA1220.13673757593418664No Hit
ACCGGAGGGATTGTCTACAGACTGCATTATTTTCCCTCGTTAGTTAAGTTTC1220.13673757593418664No Hit
GTCGACGGGGAAAGAAATTTCGTAAATGTTTGAAGGGTGAGTTTCATCAGCT1170.13113357692049046No Hit
GGCACCGGGGGACCACCATGGTAAAGAAGCGGAAAGGCCGCGTCGTGATCGA1150.128891977315012No Hit
CAGACTGGGAGCGGAGGGGTTTGGGAGTCAGGCCTGGGCAGGACCCTGCTGA1150.128891977315012No Hit
TGTCATGGGTGTGGCTCTGAAGTTTGTTTTTTAAATGACCACAAGTGTAAGA1140.12777117751227277No Hit
CTATTCGGGGGATTATTATATTATGACAATGAGTTCATGGGAATGTGGAAAT1140.12777117751227277No Hit
GTGTCTGGGATAGCTTGCCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGT1130.12665037770953352No Hit
ATTTGAGGGCAGGCAGTGGTCCCAAAAGAAAGAAGGAGGAAACAAAACACCT1130.12665037770953352No Hit
TGGCTAGGGCTGTGTTTCTAAGTCAATTCTGTAAACCTATGACACTCCTCGA1120.1255295779067943No Hit
AGCCACGGGACTGCCCTGTTTACAATAAGTATACGTGAATAATCTATTATTA1110.12440877810405504No Hit
TCACACGGGATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCC1110.12440877810405504No Hit
AGCAACGGGATTAATGAACTAGGTATCCAACATAACAAGATAAACATAAAAA1100.12328797830131581No Hit
GTAACGGGGGAGTTTATGGGGGAAATAGGTAATACAAACATTTGACAATGAA1100.12328797830131581No Hit
AAGCATGGGAGCTGTGTTTGAGGAAATTCACCTAAGAGACTGTCATCTGAGC1100.12328797830131581No Hit
CATATAGGGAAGAATTAAGCTGTTAGTTTTTCCCTAATTATCAAATAAAAAA1090.12216717849857657No Hit
GGCCATGGGATGGTGCTGGAAGTTCCAACCACTGAAATAAGGTAAGAAAAAG1090.12216717849857657No Hit
GAATTTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1080.12104637869583734No Hit
GGTACTGGGACTCCATTGTATTGCCTTTGGTCTTCTGTCATGGATCAGTTTA1080.12104637869583734No Hit
ATCGACGGGAGGTGAATGCAGTGGTTCTCCTTCATGCAACAGCAGGGGCAAG1070.1199255788930981No Hit
GGGTCCGGGTTTATTATTAAAATATAAATATTCTATTAGCAAAAATATATTC1070.1199255788930981No Hit
CAGCTTCGGGATCTGATATAATTGAGAAATGTGTCCTTAGACTATGTTCAAA1070.1199255788930981No Hit
TGTTGCAGGGACTGTGAATATTGCTGGGCTGCTATCCATTCTCGTGCTGGCA1070.1199255788930981No Hit
ATTTTGGGGACAACTCAAACTCCTCAGACACACTCTGATAAATAGACATACC1070.1199255788930981No Hit
AACAAGGGGGTGGACCCGCTGGTGCGGGATGTGGCAGGAACTCTGGCTGAAA1060.11880477909035887No Hit
ACGCGCGGGTCTTGCTGGAGGGCTACAAGTCAGTGCCCTGTATGCTCTCCAG1060.11880477909035887No Hit
CTGATTGGGGCTGTGGACAAGAGTACGGGAAAAGAGAACAAGATTACTATCA1060.11880477909035887No Hit
ACGTCGGGGTCAGTTAACTCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGC1060.11880477909035887No Hit
GCCGCAGGGGACCCATCAGAGTATTATCAATATGAAGGAAATAGTGACTGAT1050.11768397928761964No Hit
CTTAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1050.11768397928761964No Hit
ATCGCTGGGGATCATGTTGATATAAATCATAATTTCAACTAGATCAAGACAT1050.11768397928761964No Hit
TATTTTGGGTGGTTGCTTAATTCCCAACACTCAATTTCCTTCTTCTAAACAC1050.11768397928761964No Hit
TCAAGGAGGGATTTAATTTGCAAATAAAGGTGGTTGGAAAATTGTACTTTTG1050.11768397928761964No Hit
AAAAAAGGGCAAACCTAATATAAAAGGGTTCATCTGTAAGTATCATTTAGAG1040.1165631794848804No Hit
TATTCTGGGCAATGCATTTTGAAAATGATTTGTGAGCTTTATTTGAAGTCAC1040.1165631794848804No Hit
AATATTGGGACCAGAGGCTCAGTGCATTGCTAAGATGGTGAATTGCTTTTTT1040.1165631794848804No Hit
AATTTAGGGGTCCTTAAGGCTGGAATTCTTAACTTATTGACCTTATATCCTA1040.1165631794848804No Hit
CCGATAGGGAAATACCACAGGGAAGCCATGATGATTCAATTATTTTTTTCAG1040.1165631794848804No Hit
GCAGTTGGGCGCATTGAAAACGATGTTGCCACCATCCTGTCTCGCCGTATTG1040.1165631794848804No Hit
TCGGCGGGTATGAAGCCAGTATCACCCTAATATCCAAACCAGGAAAGCACAC1030.11544237968214117No Hit
GTTGCAGGGGAAAGGCCAAAATATTTTTATATTGCCACAGACAAAGCCACCT1030.11544237968214117No Hit
ACTACTGGTGAACTACTCAAGATAGTCACGAAAATACTGAAAGTTTGATTTT1030.11544237968214117No Hit
TCAAATGGGGGATCGAAGGCGAGATGGCGGACGTGCTAGATCTTCACGAGGC1030.11544237968214117No Hit
CGCAGTGGGGAACATATCCGGGAATAATTGAAAAAACCAGGCAATCAAATGT1030.11544237968214117No Hit
GAACTCGGGTGACGTTCTCATAGTTGATTCGGATGAAGAAGATTCTTCAAAT1030.11544237968214117No Hit
GTAACAGGGGATCTAACGCGGGGAATACAGAAAATTAATAATAATTATTCAA1030.11544237968214117No Hit
TGATCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1030.11544237968214117No Hit
TAGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1020.11432157987940193No Hit
GATCACGGGTACACTGTTGGTGGGACTGTAAACTAGTTCAACAATTGTGGAA1020.11432157987940193No Hit
TTTGCTGGGTTCAAGAATATGGGTGGGATATGGAATGCTCTTTCAATGTCTA1010.1132007800766627No Hit
TTCGGGGAGTCATTAATTTTCACAATTCTACAATGGCTTTAGAAAGAGGAAA1010.1132007800766627No Hit
TACACAGGGGTTCTTGCCCGGTATTTCACTCGTTTGTCTCTCCTCATGACAC1010.1132007800766627No Hit
TTAGCTCGGGGGCTGCCAAACTGGAACGTTTGCGAGAGAAGGATAAGCACAT1010.1132007800766627No Hit
CTGGCTGGGACAGCAGACAATCAACAAATATGTGTGGCAAAAACTGCTAGAA1010.1132007800766627No Hit
TTTTATGGGATTGAAGCAATACGCTCTGGGGGAAAAATAACTGTTTGACTTT1000.11207998027392346No Hit
AATACAGGGCTTGATTTGTTCCTTGGCTCAAAAGTAGGGACAAAAAAGAAAA1000.11207998027392346No Hit
TTGTAGGGGATCTCTGAGTCTGTTAAGTCAGAATATCTGGGGTTGGAACCAG1000.11207998027392346No Hit
TACGCCGGGCAGTCTACGATCTGTAAAGTTAAAAAATATTTTTGAAATATTT1000.11207998027392346No Hit
TTGGTACGGGATCATCAAGAGACTCAAGAAGAGGAAGGAGCAAACCAGAAGG990.11095918047118423No Hit
TGCTTGGGGGACCTTCTCCTTCACCTTTGTCCCCACCGCAGACAGGACCAGG990.11095918047118423No Hit
CTAGTCGGGCTTCAAATATATGAACGAGCACTTGCCTTCTAATGCCTCGGTT980.10983838066844501No Hit
CTGACAAAACACATAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAA980.10983838066844501No Hit
GATATAGGGGAAAGTATTTACAGCCTGCTGTGTGTATCTTGTGTCATTTATT980.10983838066844501No Hit
GTATCTGGGAAAATGGTTACGAGTAGAGATCACAAATGTTCACAGCAGCCGG970.10871758086570577No Hit
GAATAAGGGGTGGAGAAATAGGAACAGTTTTACACTGTTGGTGGGACTGTCA970.10871758086570577No Hit
TGCTGTGGGATAAGAGAACACCAGATAATATGCTTTATTTCTTTCGATCTTT970.10871758086570577No Hit
TGAATGGGGGCTCACTGCAACCTTCATTTCCTGGGTTCAAGCGATTCTCCTG960.10759678106296654No Hit
CTCGCAGGGAGCTGCCTTCCAAGCAGTTCCTCTGGAATTTCCAAGAATAATC960.10759678106296654No Hit
TGTATGGGGAACATTGTTTTTCCAATATATTAGTATTACAAGTGCCTAAAAC960.10759678106296654No Hit
GGATTAGGGGAACTCTTTGACATCTAAAGATCCAGATATCAAGGCACAATAT960.10759678106296654No Hit
GATGCTGGGCGGCAGTGGTTCCATAAGATTATAATACTGTATTTTTACTATA960.10759678106296654No Hit
TGTGACGGGTCTCTGAAGGGGATTATGTCAAAGGAATATATTTTATAAAAGC950.1064759812602273No Hit
TACCTGGGGATTTCTTGTGAATATAAATCTGTCATTCTATTAGCATGTGCAA950.1064759812602273No Hit
TTCATAGGGATTTCAGTACAGACAGCCTGTTATTTAACATAGACTCACAACA950.1064759812602273No Hit
AGAAGTGGGGTGTTGTCATTGTGGGAATTTGGTTAAATATTTTGTCTCAAAT950.1064759812602273No Hit
CGCTTCGGGATGAAAATATATTGGATTATTTTTGAGAGAATGAATCTTTACA940.10535518145748807No Hit
TTCACAGGGGAAGCGGAGGGAGTCGAGCCAGCGTCGCCGCGATGGTGTTGTT940.10535518145748807No Hit
GTTAAAGGGATTGTTACTGGGGTGTATCATGACTTTAAGGAAAATTATCAAA930.10423438165474883No Hit
TTTAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC930.10423438165474883No Hit
TTTGTCGGGACCTTGCAACTTTGTTGCTAGCTTGAGGTTGATTATTGTGGTT930.10423438165474883No Hit
CGGCCTGGGACCTGGAAATTAAATTCCAGCAATGACAAAAGTTTTGTGTATT930.10423438165474883No Hit
TCTTCGCGGGGAAACTGAGGTGCAGAAATGCCATATGTTGACTAAGGATAGG930.10423438165474883No Hit
CAGCAACGGGAGGTTGGCCAGAGGGTGTGACCCAGTTACCCTTTAACCCCCA930.10423438165474883No Hit
ACGTAAGGGTTGCTTAATTATTGCAGCAACTCTGTGATCTTTATTATTGCCA930.10423438165474883No Hit
TCAAACGGGGAGGACTTCAGTTGCCATGGAGACTTAAATACAGACCAACTGA920.1031135818520096No Hit
GTAACCGGGAACTTCTAAAACTTCCAAAAATGACTTCCAAAAACTTCTTCCA920.1031135818520096No Hit
AGCCTGGGCCGATCTTTAGCGGGGGACTTGCTCAGTAAAATCCTGACTGTTG920.1031135818520096No Hit
TAGCCGGGGCCGATCTGCCAAGGGATTATTTAAAACTTTTAGCTAGAGTTCT920.1031135818520096No Hit
TCGTACTGGGAGTTCCTTTTTACTGTTGAATATTGTGTGACCATTGCACGAA910.10199278204927037No Hit
CTTATCGGGTTAGGGGTCAGTCCTTGAATTGAGACCCATGTTATCTTTGGGA910.10199278204927037No Hit
CGGCTTGGGTACATTAATATATGATCTTCCATGACGAGGTCTGAAATGAACA910.10199278204927037No Hit
GCCTGGGGAGCACTGCAAAGTTTCCTAATCAGCAGCCCAGCTTTCCCTCCTA910.10199278204927037No Hit
TTAATAGGGGAGGAGGAGTGACGGGAACCGGGAGCCGGCTCTATGCGCCTGG910.10199278204927037No Hit
ACCTGGGGGACTACAGGTATTATGTAGCCAGCTGTCTGCTTTTGTAAATAAA900.10087198224653113No Hit
TATTGAGGGCAAACTATCTATAAACATATATATACATATATACACATGAACA900.10087198224653113No Hit
ATGCTCGGGCTGTTCTCACTGATATATTTAATATGATTTTCTGATTTAGTCT900.10087198224653113No Hit
GCCTATGGGGAGTGGCCCCACCTGTGTCCCCGATGCTGCCGTCACCTGGTCC900.10087198224653113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)