FastQCFastQC Report
Sat 31 Dec 2022
SRR5848696.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848696.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76547
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC6040.7890577031105073No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT6030.7877513161848277RNA PCR Primer, Index 40 (95% over 22bp)
CTACAGGGGATCTAAAAGAAGGCTGTATTCTAGAAGTTGTTGTGTTTTTTTT1360.1776686218924321No Hit
TACGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1310.17113668726403386No Hit
TGCAATGGGTAAAGGGATGGAGGAAGATCTGCCAAGCAAATGGAAAACAAAA1270.16591113956131526No Hit
AGGCCTGGGAGGATACCCTTGACAAATACTGTGACCGGGAGTATGCTGTGAA1160.15154088337883914No Hit
CCGCTGGGGCTGCACCAAGGAATTCCAGGCCCGGTGAGGGAGCTGGGTCCAT1120.14631533567612054No Hit
GCATGTGGGGTTTCTTTTTGTAGAATTCTTAATTTTCTTTTGGATATATACT1100.14370256182476127No Hit
AATTTAGGGAAATCAGTGTTTCTGCAGCTGGACTTGATAAATGAATAATGGA1060.13847701412204266No Hit
TAATCCGGGCATATGCTAGGTAGCAAATGGGGTGATATTTTTATAGAAGTGG1040.1358642402706834No Hit
TACCATGGGAATTGGGTTCATTTCAAGAATACTGTAAAAATTTTATTTAAAT1040.1358642402706834No Hit
AAACGCGGGGCACCAGGCCTTCACCAGTTTTGCCTTTGGGCAGCCTGACAAC1040.1358642402706834No Hit
ATCACTGGGTCATGAGGTCTGGCCCACGTGGGCAGTCACTCGGCCACTATGT1040.1358642402706834No Hit
CCTGCTGGGCTATTGTACAGTTTCTTGGGGATGTGGTGTTTATCCCGGCAGG1040.1358642402706834No Hit
GATACAGGGATACACATGTGTATGTGTGTGTACACACGCATATATACATACA1040.1358642402706834No Hit
GTTGCCGGGGAGTTCTCGCGAGGTTTCGTCTTCCCGGAAGCGTTGGAGGACA1040.1358642402706834No Hit
CAGCGTGGGCATTTGAAAACTCTGGAAGTTTCAAAGAAAGCTGGATGTAAGC1030.13455785334500373No Hit
TTTCCAGGGAGCCCACATAGCCAAAGCAAGACTAAGCAAAAAGAACAAATCT1030.13455785334500373No Hit
AACAATGGGATCTGCCTGAAGAGCTTTAAACTGCACTTCCCTCTCCTGCGCT1030.13455785334500373No Hit
TATACAGGGGGACTGCAATTTTTGTTTTGTATTGTTTGTGTTTTTTCTTGAA1020.13325146641932406No Hit
GCTCAGGGGACCCATTGCTTTGCAGAACACCAGACATGGGTGCTCCAATCCA1020.13325146641932406No Hit
GTTCATGGGGTGGCCCGGAACCCCGTGATCAACATCGCCTCCATGTTGGGCT1000.1306386925679648No Hit
AGGCTGGGGACATTGTAATCCCTAGAGCAACCACAAAGAAAACTATTAAAAA990.12933230564228512No Hit
AATGCAGGGATGATTTTTAAATTTGTATTTTATATTGTTTTATCTATCTGTC990.12933230564228512No Hit
GGCGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.12933230564228512No Hit
TAATGTCGGGAGTGAGCGATAAGAATGAACAGCAGCTTGGGAGGGGCCGGGA980.12802591871660549No Hit
TCATCTGGGCGAGTATGTCGGGGAGCTGATCTCTGATGCTGAGGCTGATGTG980.12802591871660549No Hit
AGCCTGGGTCACAGAGTGAGACTCCATCTCAAAAAAGAAAAGATACACCATG980.12802591871660549No Hit
ATTCCCAGCAACCCCAGAGTCCCAGCGGCGGTGCCTGCTTCTCTCTTTCCAG980.12802591871660549No Hit
GAACGGGATTATAAACAAAATAGGAAGCTAGAAATATTTGGGATCACAGTAA970.12671953179092585No Hit
TGCACAGGGTCTCTAGGTTGGGGTGTTACCAAGTTTATATGTGACAGTTGAA970.12671953179092585No Hit
AAGCCTGGGCAACAGAGACTCTGTCTCAAAGAAAACAACAACAACAACAACA970.12671953179092585No Hit
ACGTGGGGGAAAAAAAGCATTTTTCTAAATGCTCAAATGTTATCAAAATACT970.12671953179092585No Hit
TGTCTTGGGCTCTTCCGATCTGTTGCTGGGCAGTGGAGGAGATGCCCATGAA970.12671953179092585No Hit
AAAACAGGGCCGATCTAGGTTTGGGGAAAGATGACTGCTTAGACGTGCGGCG970.12671953179092585No Hit
ATGGGAGGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGT960.12541314486524618No Hit
AATGCAGGGCAAGTGGGGTGCCCCAGCTGGACACATAGCATCACTAAAAGAG960.12541314486524618No Hit
GCTCTGGGGGTTCTAGGAGGAGGTCCAGCTAGTGCAAAAGCCTTGAGATGGG960.12541314486524618No Hit
CCAATAGGGAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAA960.12541314486524618No Hit
AACAGTGGGGAACTTGCTGCTTTGTGAGTTCCATTTCACCTTTTATTTCCAT950.12410675793956653No Hit
ACACCCGGGATGTGTATAACACTCAGAATTGGGCATTGATCCTTAAAGCTTC950.12410675793956653No Hit
TGCTCAGGGAGTCTGGGACGCGCCGCCGCCATGATCATCCCTGTACGCTGCT950.12410675793956653No Hit
TGTCAGGGGATGCTCATTGTAGAAAATCCAATAGGAAAAAAAAGCATCTAAA950.12410675793956653No Hit
GGGGCTGGGTATTAAATAAACTTTAAAAGTACAGATATGTTCCTATTCAAAT950.12410675793956653No Hit
GCTGCAGGGGACGCTCTTCCATCTTAGCAAGGGAAATAACAATTAGTGTGTC950.12410675793956653No Hit
ATAGTGGGATCTGATGTTAATTTATATTCAAATGATCTTCAAATTTATTTTA950.12410675793956653No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC940.1228003710138869No Hit
ATTACGGGGTAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCT940.1228003710138869No Hit
ACACTTGGGGGGACAAATGCGTTTCCACATGCGAGAAAATGAAGGTGGACTT940.1228003710138869No Hit
CTGCACCCCGACCTGTGGTCAATGTGTCTGGAGGTCCCCTCCTTTACAGCCA940.1228003710138869No Hit
GCACAGGGGATTCACAGGTGACTGACAAGTCTTTGAAAAGAATGGGATCTGC930.12149398408820725No Hit
ACGACGGGTAGATATCTATCGCTATAGAATAATGCATTAATAAAATGAGGCT920.1201875971625276No Hit
TCAAATGGGCCAGATGTAGTTTTGTTGTTTTTTTTTAAATAAATAAATGTGA910.11888121023684795No Hit
TTGCGTAGGGGTAAGGGTAAATGACATGATTGATATTATTGTACAGTTTTTA910.11888121023684795No Hit
ATCATCGGGATATTTGGTTGATGTCTCTTAGATCTCTCTTAATCTTTTTCTC910.11888121023684795No Hit
TTTGTGGGGGCATGGCATCCCCCCCAGCTGCTGCATGAACGAAACTGATTGT910.11888121023684795No Hit
AGGCCTGGGAAGATGAGACCTGAGAATCAACATTTGATTGGGCAACATGAAG910.11888121023684795No Hit
GCAGGAAGTAAACCTTTCATTTACCAGGAGGTAATTGATCTGGGTGGTGAGC910.11888121023684795No Hit
CTTAAAGGGGAGAACGTCTGAAAATTTCTGTAACACAGTAATTACTATAATT910.11888121023684795No Hit
TTACTTGGGTCTCAATGAATTGAAGAAAGCCAAAATTATACCAAGTGCCCTC900.11757482331116831No Hit
AGTCTGGGGATCTGTATTAGGGTTCTCTAGAGGGACAGAACTAAGGGGAGTT900.11757482331116831No Hit
GCAAGGGGCAGCCACTTTCCTTACCTCTACTTTTAAAAGCTTTGCATGTCTT900.11757482331116831No Hit
TACTTAGGGACAGAAACACATAGTGGGCTGGATTGGTTCATGGGCCACAGGT900.11757482331116831No Hit
CGTGGCGGGACAGCCGCGGCGGGAGCCCCGCAGCCCCGGGCTGGGCCCGCGT900.11757482331116831No Hit
CGAAACGGGTTATAATTAACTGGTGATATCACAGATATTCCAAGATATAATT900.11757482331116831No Hit
GATGTAGGGACAGTCTATGTGGGATATTAAAATTTCATGGCGAGGGGATTTC900.11757482331116831No Hit
TACGTAGGGACAATCAAATTAGTCTCCAATTTTCTAAGACCAGATCTAGTTA890.11626843638548866No Hit
AACATCGGGATGCCTAGTAAGCGCCACAGGTGACTCTGATGCAGGCGCCACA890.11626843638548866No Hit
TTCGCTGGGTGAAAGAACAGCAGTGGTGGCAACTATGCCTTGCCTTCTGGAG890.11626843638548866No Hit
GTAACAGGGCGATCTGGGTACGGGAGTGGACACTTTCTGTCCTCCTACTTAA880.114962049459809No Hit
GCAATGGGGTAAAGTATTAATAAATCAGTCATCTCTATAAAAATAAGACTTT880.114962049459809No Hit
AGAATCGGGGTGCCGGGGATGGGAATCACTGAAATATTTTTGTAGATTAATT880.114962049459809No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCCTGTCTCTTATAC880.114962049459809No Hit
ACGTTAGGGGCACCTCCGGTGGGCCTGGCAAACTGGTTGAAGATAGAGTTGT880.114962049459809No Hit
AGCTGCGGGCAACTGGCTGAGAATCTTTTTATTTGCTGATTTGTCTCTTGTG880.114962049459809No Hit
GCACGCGGGCTTCTCTATATTCATTTCACAAGAGCTGGCTTAGGTGGGGTAA880.114962049459809No Hit
TGTTCAGGGATTACAACATACAATGTTGTGTCATGGTATTTTCCATTTTACG880.114962049459809No Hit
TGTACTGGGGGACATACTGATTGTATACTTTGATTCTTAAACCATGGAGATG870.11365566253412937No Hit
ACCTAAGGGTCTGAAAGTGGGGAAGTGAGATTCCACTCTGTAGAACCCTACA870.11365566253412937No Hit
GCATTAGGGCTGCTGCCCTGTGAAGAAGATGCCTTTCTTCCCCTTTGCTTTC870.11365566253412937No Hit
AAATAGGGGCAGGCCTCCACCTCCTGCCTCCCAGCACCCCCTGCTGAGTCTG870.11365566253412937No Hit
GTTGCTGGGGATAACTGGTGGCAGTTCTGTGCCTAAGCTGACATCACCAGTT860.1123492756084497No Hit
GTGGGTGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA860.1123492756084497No Hit
AAACATGGGTGACTGCATAAATCAAAAAACATGTCTTAGATTTTGGGGTGTA860.1123492756084497No Hit
GGTTATGGGGGGAGTAGGGCCGAGCGCGGAACGCGAGGGGCTGCTGGGGTGT860.1123492756084497No Hit
TACATTGGGGATAGAATACAGTGCGATTTGGAAAGACACCACAAAAACTCAT850.11104288868277006No Hit
ACTGCCGGGTAACCGACGTTGAAGATAAGTCTGATTCCGTTATGGTTTTTCT850.11104288868277006No Hit
ACTGCAGGGACAGTGGTGACTCTGGACAAGGAGACTCCCGTAGCATGACCCC850.11104288868277006No Hit
ATGCTAGGGCACATGAGGGTCTAAATGCATTAGCAAACACATTTATACATAT850.11104288868277006No Hit
GAATAAGGGAGGAGGTCCAACTGCCGTAGACAGGAAGGAATTAAAATTGTCC850.11104288868277006No Hit
GAACAAGGGTAATTACATCTGCAACAATCCTGTTTCCAAATAGGATCACATC840.10973650175709043No Hit
TAGTATGGGAGAATTAGCTTAAACAATGAGTCCTATGTAGCTTTTAAGACAT840.10973650175709043No Hit
ACAGCCGGGCTGCAGCAATCTTTGAAAGACTTCCAAAGAATGTTAGAGCACA840.10973650175709043No Hit
TCGTCAGGGACCAAAACCAGACAAGAACACAACAAAACAAGAAATCCCTAAT840.10973650175709043No Hit
TATCGGGGGGCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGC840.10973650175709043No Hit
TACCCAGGGTTTGACATTGATGCCAATGGTATACTCAATGTCTCTGCTGTGG840.10973650175709043No Hit
GCGTATGGGCACGATTTCAGCCATGAATCAGGCATAAATATTCTGATGGTTA830.10843011483141077No Hit
TACAAAGGGAAGAAAAATGTGAATTTGGTGGTTTTATTCTATCGGTATAAAG830.10843011483141077No Hit
TTAGTGGGGAAGCAATGGTTACAAGTAAAGAAATCATCTCATTTGGAAGATA830.10843011483141077No Hit
TAAATGGGGGCAGATGAGGGCCACAAAGTGATTGTTGGTTGTGACAATGAGG830.10843011483141077No Hit
GAAGCTGGGCTGCACATCAAGTATCCTAAGGACAGGGGATATCAAGATGCCA830.10843011483141077No Hit
CTTGTAGGGTTAAAGGGAATTTACACTGGGAGAGTGCTTAGCCTTCAAGAAT830.10843011483141077No Hit
CAATGCGGGATCTCTTAAATCTCCCTTGAAGCCAGAGTCACAGCTAGATCTT830.10843011483141077No Hit
TTGCTTGGGGCCTAGGTTGGTAACTATGTTTCTTTATATTTTGTGCAAAGTA830.10843011483141077No Hit
TACCTGGGGATAAGGGATAGGCTTGGTGGGATTCCAGCTTGGGGCAGTGGCA830.10843011483141077No Hit
ACTAGAGGGGAAAAGGCTAAAGATAGATAACTTTAAATATACAGAGAGATTA830.10843011483141077No Hit
TGTGTGGGACTGGTCATCAGCTCATGAGTTTGTCTACTGCAAATGCAAAGAA830.10843011483141077No Hit
GATTAAGGGACAGGGGGTAAAGCAAAAGGAGGAAGAATTCTACTCAGGCCAT820.10712372790573112No Hit
AATGTTGGGCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTTGG820.10712372790573112No Hit
GTAGTGGGTGACTTCCAGAAAGTTGAGATCTTTTGACCATTTTTTCTCGTGT820.10712372790573112No Hit
TTGGCAGGGGATGTTCCTGTTGGTTCTCGAGGGGACTGCTATGATAGGTACC820.10712372790573112No Hit
CCTTAGGGGGATGGGAGGCGGCTGGTGGTGGGCTCGGGCCGCTCGCCTTGCC820.10712372790573112No Hit
GTTGCGGGACCATGGGATGATGCAACATGAGGTTCTTCACCAGATGCTGGCC820.10712372790573112No Hit
TTGGCGGGCGCCTGTAATCCCTGCTACTGAGTAAGGCTGAGGCAGGAGAATC820.10712372790573112No Hit
AGCTGTGGGGGAGAAGAAAATGTAAAGCAGGTGGTCCCCAGCCTCCTGGGCA810.10581734098005147No Hit
CGTGGGAGAGTTTCTCAGCATCTTTCTTCCTGAGTGGATGGCATTATCCCTA810.10581734098005147No Hit
CTCTTGGAAAGGGATACCTGTATTTTCCTAACTAAGCCAGTTAGTTTTTCAC810.10581734098005147No Hit
GTTGGCGGGGAAGAGTGAATGTAACTTATTACCCACAGGGACAATTCCAAAT810.10581734098005147No Hit
GGCATTGGGAGGCGTGGGTCAATGGAAGTATTGTTTTACAAAATGGAGATAA810.10581734098005147No Hit
ACGGAGGGGGGTGGTGGGCGTCTGAAAAGTCGCCACCATGAGGAAGCTCTTC810.10581734098005147No Hit
GCAGCACGGGATTGCTACAGATGTAGCCTCCCGTGGGCTAGGTTTGTATAGA810.10581734098005147No Hit
TAACTTGGGGACCTCCTCAGCTTTTGTAGAAACACTAAAGAAGACGTTGGAA800.10451095405437183No Hit
ACCAAAGGGCGATCTTCTCGCGGGTTCTCAAGTCCTGGCACACCATACACGA800.10451095405437183No Hit
ACTGAAGGGCACCTCCAGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGGG800.10451095405437183No Hit
TTAGCAGGGGCTTTTCTCAATTTTGGAGAAGATGTTTAAATAGTTCTGTTGT800.10451095405437183No Hit
ACTTGAGGGATCAATATTGTGAAAATGGCCATACTGTCCAAGGTAATTTATA800.10451095405437183No Hit
TAAGTGGGATTGCAATATTTTCATATACAGACACTAAAAACAGTTTTCAAAG790.10320456712869218No Hit
TCACCGGCTTCCGATCTTAGCCGGGGAGAAATACATGATAGCACCAAATGCC790.10320456712869218No Hit
ATAGTTGGGGGGATTAAAGGCATGAGCCACTGCGCCCAGCAGGAATGGTTAT790.10320456712869218No Hit
ATACTAGGGGCTAATTGCAAATCATCCTTATCAAGTCCAAAGTATAAGTCCT790.10320456712869218No Hit
GGTGCTGGGTGTGCATCCCTTGGGAGAAGAACAAGGGTTGTTATATTCTGAA790.10320456712869218No Hit
CAAAAAAAAAGAAAAGATTCATGATGCTGCTGCTCCCAGAAGGTTTGCTGGA790.10320456712869218No Hit
AAATGCGGGATGGATGGCATGCAGCAGCACCCAAGTATTCTTCATTCTTTGC790.10320456712869218No Hit
TTGCTTGGGCACCATCTCCATTTCTGTCCTGCTGGGTCAAGCCACCATCCTC790.10320456712869218No Hit
CGATTAGGGATGAGAGCTGGTTGTTTAAAAGTTTAAAAGCACCTCCCCCTTC790.10320456712869218No Hit
GCCACTGGGGAGAAAAGGGGTGAAGAGAAAAAACCAGATTATATAGAAAGGA790.10320456712869218No Hit
TCAAACGGGTATTTTCCAAGATAATAGTTTTCTTCAAAGAAACCAAAACAAA790.10320456712869218No Hit
TGCCAAGGGATCAGAAAACTGAGTGGACCATGGTAAATTCTTAATTGGTTTT780.10189818020301253No Hit
CTTTCGTGGGGATGTCCAAGTTAAAGCAAATTTAGCACCCTATCCTTATTGG780.10189818020301253No Hit
CCACCCTTCCCCCTTGCTACCAGTCACTTAAACCAAGTCTGTGTTGTGGCTT780.10189818020301253No Hit
GTTCCTGGGCATGATCTAGAACCCACTGAATGGGTCTTTTGGAAGCAACACC780.10189818020301253No Hit
CAAGCAGGGAGGCTTTATTGCTTTGAAAACAACATTGGCCTTTTGTATCTCA780.10189818020301253No Hit
TTAAAAGGGGAGAGACAGAGAGGGAGAGAGAGAGCAAGAGGGAGAGTGTGTG780.10189818020301253No Hit
GAAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA780.10189818020301253No Hit
TTTGATGGGGGTCCCAACCATGTGATGTCTGGAGCATAGGATGCATTCTTAT780.10189818020301253No Hit
TGACGGGCGAAGCAAATATTGGCAAAATTTAGGGAGAGAATACGTAGTTCTG780.10189818020301253No Hit
AAGCCTGGGGCACTCATGATGTAATGGGAACTTCAAGTGGGTAATCACACTT770.10059179327733289No Hit
TAGTTCGGGAAGATATATGTGTTATTTAGATGTACCTAAAGCATAAAGATAA770.10059179327733289No Hit
TAATTCGGGATGACAGCTGACAGTTTTTCAGAGGTCGCACACAGTGACTCTC770.10059179327733289No Hit
TGAAATAGGGGGATCCAGAAAATAGTGTGTTACAAAATTTGAAAGATGTTTT770.10059179327733289No Hit
TGTTCGGGGAAATCATAAAGAGCCTCTTACGGCTAGCTAAGGAGTATGCATT770.10059179327733289No Hit
ATTACTGGGCCTCACCAGCAGTGTGTCTTTGGACAAGTCACTAAAACTCCGT770.10059179327733289No Hit
AGGGCAGGGGACCTAAGAAAAATAATGAGAACAAGCTGTTGCAAGCTCTTTT770.10059179327733289No Hit
TGCATAGGGATATCCCGTTGCCATTATACTTTTGTCAAGACTCAGAACGTAC770.10059179327733289No Hit
AGTGGCAGGGAAGAAAGCTTAAGGAAAATGAATTAATGTAAATTTTAATAAG770.10059179327733289No Hit
GCTTTCGGGGGCGATGCCAAAGCCAGGCGGGCTGCTGGGGTGTGAGCATGGA770.10059179327733289No Hit
GACTTAGGGAACTCACTGCGGCCTTGAACACCTGAGCTCAAGCGATCCTTTC770.10059179327733289No Hit
GCGGCAGGGATGACAAAATGAATGTGAATATTCTTTCTAAAAATTTAGAAAA770.10059179327733289No Hit
TGTCACGGGAAGCCACTGACATTTAATATTTACTGAAGCCATTCCTTAGACA770.10059179327733289No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)