FastQCFastQC Report
Sat 31 Dec 2022
SRR5848694.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848694.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75551
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT6190.8193141057034321RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC5680.751810035605088No Hit
TACGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.1707455890722823No Hit
TAATCCGGGCATATGCTAGGTAGCAAATGGGGTGATATTTTTATAGAAGTGG1190.1575094968961364No Hit
GGCGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1170.15486227846090722No Hit
CTACAGGGGATCTAAAAGAAGGCTGTATTCTAGAAGTTGTTGTGTTTTTTTT1120.14824423237283424No Hit
GCATGTGGGGTTTCTTTTTGTAGAATTCTTAATTTTCTTTTGGATATATACT1110.14692062315521964No Hit
TAAATGGGGGCAGATGAGGGCCACAAAGTGATTGTTGGTTGTGACAATGAGG1110.14692062315521964No Hit
AGGCCTGGGAAGATGAGACCTGAGAATCAACATTTGATTGGGCAACATGAAG1110.14692062315521964No Hit
CCTGCTGGGCTATTGTACAGTTTCTTGGGGATGTGGTGTTTATCCCGGCAGG1080.14294979550237588No Hit
TTACTTGGGTCTCAATGAATTGAAGAAAGCCAAAATTATACCAAGTGCCCTC1050.13897896784953212No Hit
TGTCTTGGGCTCTTCCGATCTGTTGCTGGGCAGTGGAGGAGATGCCCATGAA1050.13897896784953212No Hit
ACGACGGGTAGATATCTATCGCTATAGAATAATGCATTAATAAAATGAGGCT1030.13633174941430293No Hit
AATTTAGGGAAATCAGTGTTTCTGCAGCTGGACTTGATAAATGAATAATGGA1020.13500814019668833No Hit
GTTGCCGGGGAGTTCTCGCGAGGTTTCGTCTTCCCGGAAGCGTTGGAGGACA1020.13500814019668833No Hit
CTTAAAGGGGAGAACGTCTGAAAATTTCTGTAACACAGTAATTACTATAATT1000.13236092176145914No Hit
AAAACAGGGCCGATCTAGGTTTGGGGAAAGATGACTGCTTAGACGTGCGGCG1000.13236092176145914No Hit
AAACGCGGGGCACCAGGCCTTCACCAGTTTTGCCTTTGGGCAGCCTGACAAC990.13103731254384454No Hit
TTGGCGGGCGCCTGTAATCCCTGCTACTGAGTAAGGCTGAGGCAGGAGAATC990.13103731254384454No Hit
TCATCTGGGCGAGTATGTCGGGGAGCTGATCTCTGATGCTGAGGCTGATGTG980.12971370332622997No Hit
GATTAAGGGACAGGGGGTAAAGCAAAAGGAGGAAGAATTCTACTCAGGCCAT970.12839009410861538No Hit
GTGGGTGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA970.12839009410861538No Hit
TGCTCAGGGAGTCTGGGACGCGCCGCCGCCATGATCATCCCTGTACGCTGCT970.12839009410861538No Hit
ATCGACGGGGTTTGATACCAGACAGAAAGCTGACAGTCTGTTCTTTGTAAAC970.12839009410861538No Hit
AGGCCTGGGAGGATACCCTTGACAAATACTGTGACCGGGAGTATGCTGTGAA970.12839009410861538No Hit
GAACAAGGGTAATTACATCTGCAACAATCCTGTTTCCAAATAGGATCACATC960.12706648489100078No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC960.12706648489100078No Hit
TTAAGAGGGGAGGGGTTATCTTTGGCATGTGGTCAGCAGGGTGTCCCACTAC960.12706648489100078No Hit
TGTCAGGGGATGCTCATTGTAGAAAATCCAATAGGAAAAAAAAGCATCTAAA960.12706648489100078No Hit
CGAAACGGGTTATAATTAACTGGTGATATCACAGATATTCCAAGATATAATT960.12706648489100078No Hit
CAATGCGGGATCTCTTAAATCTCCCTTGAAGCCAGAGTCACAGCTAGATCTT960.12706648489100078No Hit
CAGCGTGGGCATTTGAAAACTCTGGAAGTTTCAAAGAAAGCTGGATGTAAGC950.12574287567338618No Hit
ACGTTAGGGGCACCTCCGGTGGGCCTGGCAAACTGGTTGAAGATAGAGTTGT940.1244192664557716No Hit
AGTCTGGGGATCTGTATTAGGGTTCTCTAGAGGGACAGAACTAAGGGGAGTT930.12309565723815702No Hit
CGTGGGAGAGTTTCTCAGCATCTTTCTTCCTGAGTGGATGGCATTATCCCTA930.12309565723815702No Hit
GCACAGGGGATTCACAGGTGACTGACAAGTCTTTGAAAAGAATGGGATCTGC930.12309565723815702No Hit
GCTCTGGGGGTTCTAGGAGGAGGTCCAGCTAGTGCAAAAGCCTTGAGATGGG930.12309565723815702No Hit
AACAGTGGGGAACTTGCTGCTTTGTGAGTTCCATTTCACCTTTTATTTCCAT920.12177204802054241No Hit
ACACCCGGGATGTGTATAACACTCAGAATTGGGCATTGATCCTTAAAGCTTC920.12177204802054241No Hit
ATGCCCAGCAGGGTTTAAGCTACAGCAAGTCAGGATGTCAGTCGTTGAAGAT920.12177204802054241No Hit
AATGCAGGGCAAGTGGGGTGCCCCAGCTGGACACATAGCATCACTAAAAGAG920.12177204802054241No Hit
ACGTGGGGGAAAAAAAGCATTTTTCTAAATGCTCAAATGTTATCAAAATACT920.12177204802054241No Hit
ACTAGAGGGGAAAAGGCTAAAGATAGATAACTTTAAATATACAGAGAGATTA920.12177204802054241No Hit
TACCATGGGAATTGGGTTCATTTCAAGAATACTGTAAAAATTTTATTTAAAT910.12044843880292783No Hit
AATGCAGGGATGATTTTTAAATTTGTATTTTATATTGTTTTATCTATCTGTC910.12044843880292783No Hit
AACAATGGGATCTGCCTGAAGAGCTTTAAACTGCACTTCCCTCTCCTGCGCT910.12044843880292783No Hit
GATACAGGGATACACATGTGTATGTGTGTGTACACACGCATATATACATACA910.12044843880292783No Hit
CTGGACGGGTTTCTATAACTCTGTGCCCTTCATGAATGATCAGTCAGATGCC900.11912482958531323No Hit
GTTGCTGGGGATAACTGGTGGCAGTTCTGTGCCTAAGCTGACATCACCAGTT900.11912482958531323No Hit
GTAGTGGGTGACTTCCAGAAAGTTGAGATCTTTTGACCATTTTTTCTCGTGT900.11912482958531323No Hit
ATTCCCAGCAACCCCAGAGTCCCAGCGGCGGTGCCTGCTTCTCTCTTTCCAG900.11912482958531323No Hit
AAGCCTGGGGCACTCATGATGTAATGGGAACTTCAAGTGGGTAATCACACTT890.11780122036769865No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCCTGTCTCTTATAC890.11780122036769865No Hit
TACTCAGGGGAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAAC890.11780122036769865No Hit
ATTACTGGGCCTCACCAGCAGTGTGTCTTTGGACAAGTCACTAAAACTCCGT890.11780122036769865No Hit
ATGCTAGGGCACATGAGGGTCTAAATGCATTAGCAAACACATTTATACATAT890.11780122036769865No Hit
AACTTAGGGCAGTGAAGGTTGTGAACTTTATAGTGAATAGATGAGACTGACA890.11780122036769865No Hit
GAACGGGATTATAAACAAAATAGGAAGCTAGAAATATTTGGGATCACAGTAA880.11647761115008404No Hit
TTGCGTAGGGGTAAGGGTAAATGACATGATTGATATTATTGTACAGTTTTTA880.11647761115008404No Hit
TTTGTGGGGGCATGGCATCCCCCCCAGCTGCTGCATGAACGAAACTGATTGT880.11647761115008404No Hit
TGTAAGGGGGATGGCCCAATGTATTTGAGACATCTCCTCATGTAATTCAGGC880.11647761115008404No Hit
CCAATAGGGAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAA880.11647761115008404No Hit
TGTGTGGGACTGGTCATCAGCTCATGAGTTTGTCTACTGCAAATGCAAAGAA880.11647761115008404No Hit
AGGCTGGGGACATTGTAATCCCTAGAGCAACCACAAAGAAAACTATTAAAAA870.11515400193246945No Hit
GTTCCTGGGCATGATCTAGAACCCACTGAATGGGTCTTTTGGAAGCAACACC870.11515400193246945No Hit
TGAAATAGGGGGATCCAGAAAATAGTGTGTTACAAAATTTGAAAGATGTTTT870.11515400193246945No Hit
CGTGGCGGGACAGCCGCGGCGGGAGCCCCGCAGCCCCGGGCTGGGCCCGCGT870.11515400193246945No Hit
TGTAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC870.11515400193246945No Hit
TTCGCTGGGTGAAAGAACAGCAGTGGTGGCAACTATGCCTTGCCTTCTGGAG870.11515400193246945No Hit
AGAATCGGGGTGCCGGGGATGGGAATCACTGAAATATTTTTGTAGATTAATT860.11383039271485487No Hit
AGCTGTGGGGGAGAAGAAAATGTAAAGCAGGTGGTCCCCAGCCTCCTGGGCA860.11383039271485487No Hit
TGTTAAGGGATGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCACC860.11383039271485487No Hit
TTTCCAGGGAGCCCACATAGCCAAAGCAAGACTAAGCAAAAAGAACAAATCT860.11383039271485487No Hit
ATCATCGGGATATTTGGTTGATGTCTCTTAGATCTCTCTTAATCTTTTTCTC860.11383039271485487No Hit
TACCCAGGGTTTGACATTGATGCCAATGGTATACTCAATGTCTCTGCTGTGG860.11383039271485487No Hit
GTCACGGGGAGACTATCAAAATCTGTCTTGTGAAAGTAATTTATTAAAATAT860.11383039271485487No Hit
ACACTTGGGGGGACAAATGCGTTTCCACATGCGAGAAAATGAAGGTGGACTT850.11250678349724029No Hit
AACATCGGGATGCCTAGTAAGCGCCACAGGTGACTCTGATGCAGGCGCCACA850.11250678349724029No Hit
AAGCCTGGGCAACAGAGACTCTGTCTCAAAGAAAACAACAACAACAACAACA850.11250678349724029No Hit
ACTGCAGGGACAGTGGTGACTCTGGACAAGGAGACTCCCGTAGCATGACCCC850.11250678349724029No Hit
TCAAACGGGTATTTTCCAAGATAATAGTTTTCTTCAAAGAAACCAAAACAAA850.11250678349724029No Hit
TAGTTCGGGAAGATATATGTGTTATTTAGATGTACCTAAAGCATAAAGATAA840.11118317427962568No Hit
ATATTAGGGATTAGGGAAATGCATAGAAAAACCACTTTTCTTGTTCATTAGT840.11118317427962568No Hit
AGCCTGGGTCACAGAGTGAGACTCCATCTCAAAAAAGAAAAGATACACCATG840.11118317427962568No Hit
GGTGCTGGGTGTGCATCCCTTGGGAGAAGAACAAGGGTTGTTATATTCTGAA840.11118317427962568No Hit
AAATGCGGGATGGATGGCATGCAGCAGCACCCAAGTATTCTTCATTCTTTGC840.11118317427962568No Hit
TGCAATGGGTAAAGGGATGGAGGAAGATCTGCCAAGCAAATGGAAAACAAAA840.11118317427962568No Hit
AAGTGAGGGAGATTTTAATTACTGTATTTTGGTTTTCAGGGAAAGAAGTTAG840.11118317427962568No Hit
GCACGCGGGCTTCTCTATATTCATTTCACAAGAGCTGGCTTAGGTGGGGTAA840.11118317427962568No Hit
CATCATGGGGTAACACTAAGTAGAAATTATAAAAAGTCAAACATTGTGATCT830.1098595650620111No Hit
CCACCCTTCCCCCTTGCTACCAGTCACTTAAACCAAGTCTGTGTTGTGGCTT830.1098595650620111No Hit
ATACTAGGGGCTAATTGCAAATCATCCTTATCAAGTCCAAAGTATAAGTCCT830.1098595650620111No Hit
CCTACTCGGGCAGTCAGAGGTGAGGTTAGATCATAAAATTTGTAATATGATT830.1098595650620111No Hit
GATCAAGGGAGCAACCTACCCTGCTGGATATTCACTGTTTGGATACTAACTG830.1098595650620111No Hit
ATTACGGGGTAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCT830.1098595650620111No Hit
GCTGCAGGGGACGCTCTTCCATCTTAGCAAGGGAAATAACAATTAGTGTGTC830.1098595650620111No Hit
CTGCACCCCGACCTGTGGTCAATGTGTCTGGAGGTCCCCTCCTTTACAGCCA830.1098595650620111No Hit
ACACCGGGCAGATGAGTCTCTATTCCTGGCACATCCCCTTTGGAATTCTCTA820.1085359558443965No Hit
GCATTAGGGCTGCTGCCCTGTGAAGAAGATGCCTTTCTTCCCCTTTGCTTTC820.1085359558443965No Hit
CTTGTAGGGTTAAAGGGAATTTACACTGGGAGAGTGCTTAGCCTTCAAGAAT820.1085359558443965No Hit
GCAGGAAGTAAACCTTTCATTTACCAGGAGGTAATTGATCTGGGTGGTGAGC820.1085359558443965No Hit
GCGGCAGGGATGACAAAATGAATGTGAATATTCTTTCTAAAAATTTAGAAAA820.1085359558443965No Hit
GTTGGCGGGGAAGAGTGAATGTAACTTATTACCCACAGGGACAATTCCAAAT820.1085359558443965No Hit
ACGGAGGGGGGTGGTGGGCGTCTGAAAAGTCGCCACCATGAGGAAGCTCTTC820.1085359558443965No Hit
GTAACAGGGCGATCTGGGTACGGGAGTGGACACTTTCTGTCCTCCTACTTAA810.10721234662678192No Hit
GCAATGGGGTAAAGTATTAATAAATCAGTCATCTCTATAAAAATAAGACTTT810.10721234662678192No Hit
AGCATAGGGACTTTAAGGAACTAGAGAAACAAGAACAAACCAAGCTCAAACC810.10721234662678192No Hit
GCAAGGGGCAGCCACTTTCCTTACCTCTACTTTTAAAAGCTTTGCATGTCTT810.10721234662678192No Hit
CTATTTGGGCTACCCTCATGACCAAAAATTATCTTCCAAAAGTCCTACCTCC810.10721234662678192No Hit
TACAGAAGGGACCACCACACCCAGCTATTTTTTGTATTTTTACTGGAGACAG810.10721234662678192No Hit
GCCGGTGGGGTGGCTAACCATCAAATACATAGTCATCTAAGCTAAACTTTCT810.10721234662678192No Hit
GCTCAGGGGACCCATTGCTTTGCAGAACACCAGACATGGGTGCTCCAATCCA800.10588873740916731No Hit
ACAGCCGGGCTGCAGCAATCTTTGAAAGACTTCCAAAGAATGTTAGAGCACA800.10588873740916731No Hit
AGCCGCGGGCAGTCATGGAGAGGAATTAGGGGGATAATGTCTAAAAGAAAAA800.10588873740916731No Hit
TCCGTAGGGGCTCCACATGCGTTAGTTACTTATCCTGATGCTCTCCCTTCCT800.10588873740916731No Hit
TACGTAGGGACAATCAAATTAGTCTCCAATTTTCTAAGACCAGATCTAGTTA800.10588873740916731No Hit
GACCGGGTAAAAAACAATTATCCCTTACAGACTTGAACATTTGCAGACCTTA800.10588873740916731No Hit
ACTTGAGGGATCAATATTGTGAAAATGGCCATACTGTCCAAGGTAATTTATA800.10588873740916731No Hit
GGTTATGGGGGGAGTAGGGCCGAGCGCGGAACGCGAGGGGCTGCTGGGGTGT800.10588873740916731No Hit
AATGTTGGGCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTTGG790.10456512819155273No Hit
GGCCTAGGGGATGCTGTGTGTGGGACTTCGGTGAACCTATCACTCAAATAGT790.10456512819155273No Hit
TAGTATGGGAGAATTAGCTTAAACAATGAGTCCTATGTAGCTTTTAAGACAT790.10456512819155273No Hit
TTCATCGGGAACACCCTTATCTGTTAGCTCTGTCGATCCAGGGATGAAGGTT790.10456512819155273No Hit
TGACGGGCGAAGCAAATATTGGCAAAATTTAGGGAGAGAATACGTAGTTCTG790.10456512819155273No Hit
TAATTCGGGATGACAGCTGACAGTTTTTCAGAGGTCGCACACAGTGACTCTC780.10324151897393814No Hit
ATCACTGGGTCATGAGGTCTGGCCCACGTGGGCAGTCACTCGGCCACTATGT780.10324151897393814No Hit
ACAAGCGGGCGACGCTCTTCCGATCTAAACTAGGGAGGCATTGAGGCAGCCA780.10324151897393814No Hit
ATTTCGGGATTGTCAGCATTCTTGGGTGAGGATTAGCCTACCATGTTCTAAT780.10324151897393814No Hit
TCGTCAGGGACCAAAACCAGACAAGAACACAACAAAACAAGAAATCCCTAAT780.10324151897393814No Hit
CAGAAGGGGGTATTCTTGTTGAATGTAATTGATGGAGTATTAACATGTAGAA780.10324151897393814No Hit
CTCTTGGAAAGGGATACCTGTATTTTCCTAACTAAGCCAGTTAGTTTTTCAC780.10324151897393814No Hit
ATGGGAGGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGT770.10191790975632356No Hit
TAACTTGGGGACCTCCTCAGCTTTTGTAGAAACACTAAAGAAGACGTTGGAA770.10191790975632356No Hit
TTTCTGGGGGTGAGGTGCCTCCTTGGCACAGACTGTGGATGCCTCTCCCCCA770.10191790975632356No Hit
TATTAGGGGACCCCACGACAGGCCCTGGTATGTGATGTTCTCCACCCTGTGT770.10191790975632356No Hit
ACTAGCGGGCTAACCAGAAGAGGAGGGAAAAATAGTTGGAATTTAAGTTCTG770.10191790975632356No Hit
GAAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA770.10191790975632356No Hit
AGGGCAGGGGACCTAAGAAAAATAATGAGAACAAGCTGTTGCAAGCTCTTTT770.10191790975632356No Hit
TCTTAGCGGGCTCTTCCTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAA770.10191790975632356No Hit
GAGTACGGGTAAGTCAAAGATTTCCACAAAACCATCATATCCCATATCGATA770.10191790975632356No Hit
TGTTCAGGGATTACAACATACAATGTTGTGTCATGGTATTTTCCATTTTACG770.10191790975632356No Hit
TAATGTCGGGAGTGAGCGATAAGAATGAACAGCAGCTTGGGAGGGGCCGGGA760.10059430053870895No Hit
GACTAAGGGAGAGCTTTCAAGAACACACAATGCAAAGTGAGCGCAGCATAGC760.10059430053870895No Hit
CAAGCAGGGAGGCTTTATTGCTTTGAAAACAACATTGGCCTTTTGTATCTCA760.10059430053870895No Hit
TTAAAAGGGGAGAGACAGAGAGGGAGAGAGAGAGCAAGAGGGAGAGTGTGTG760.10059430053870895No Hit
CGCATAGGGATCTTCCACTTAAATACCCTCATTTGACCCTAGGAAGTTTTTT760.10059430053870895No Hit
AGATTAGGGAGATGTTGGCTCAAGTTCAGGGCCACCATCATATACCTAACAA760.10059430053870895No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)