FastQCFastQC Report
Sat 31 Dec 2022
SRR5848693.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848693.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98152
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT8600.8761920286901947RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC7660.7804222022984758No Hit
TTGCACTGGGATTTTTTAAATATATAAAAACCTAAAAAGGAATTTGTGACAT1420.14467356752791588No Hit
AATGCTGGGGATGTATTTGTTGTAGCAGCCTGAGCTAAGACAGACATATATG1420.14467356752791588No Hit
AGCAATGGGTCAGGCTCGACTTCTAATTCTAGTTCTCTTGTTATTTCTACCA1360.13856059988589128No Hit
GAAATGGGGGCACCTGACTGTCTTCTGCATCTTCTGTGGTGGCTGCTTCTAG1340.1365229440052164No Hit
GGTGAAGGGTGAAGTTAACAACGGACTTGGGAAATCTCAGGCTAAGAACAAC1300.13244763224386666No Hit
GAAACAGGGATGAATTTAAAGTTTATTATCCCAATTCTACAGAAGGATTTAA1280.1304099763631918No Hit
TTGTTCGGGCAGAGGTGGGATGCAGATCTTCGTGAAGACCCTGACTGGTAAG1240.12633466460184203No Hit
AGCTTTGGGGGGCTGTGGCAGTGGACCTGGATGGCAGGCAGAGCAGAGGACC1220.12429700872116717No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC1210.12327818078082974No Hit
GCATTAGCTTAAATGTTTATTGCTTTGTAAATCACACAATACTCTGTTATTT1210.12327818078082974No Hit
GCACCGGGATGATGACTAGAGACTATTTGTCAGTCAAAATTTGGGACTTAAA1210.12327818078082974No Hit
CAATTAGGGGAACAATGGGGGTTTCAAAACATAAAAGTGAAAGTCCTTGTGA1210.12327818078082974No Hit
CAATCTGGGCAATTTACAAAGAGAAGTTCCTGATGAAAGAAGTGTATCTTGA1190.12124052490015487No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCCTGTCTCTTATAC1180.12022169695981742No Hit
ATGCTAGGGAAACAAGTTGAATATGTTTTCATACATTCATACTATAAACTGT1180.12022169695981742No Hit
AGCTTGGGGCTAAGTCACTTGGTAAGAAAGTAAATAAACCTGTTGAGGTATA1160.11818404107914256No Hit
ACGTATGGGACCCTCTCTGGCCTTAGTTTTCTTGTTTGCTATCAAGCTTGGG1150.11716521313880512No Hit
TCGTATGGGTGCAAGGCACTGAGGAGTATCAAAGAAGCAAATATTTTCACTT1130.11512755725813024No Hit
GGTATTGGGGAAGGCAGGGGGGAAGGGACAGTCGGCCGCAGACCGCGCTGGG1130.11512755725813024No Hit
TCGTATGGGAAAATTTCTTTCAAAATAATTTTGAAAGAAATTTTCCCATATG1110.11308990137745539No Hit
TAACTGGGGGCCGCCCAGTGTCCCAGAGTCCCTTTCGAGGCGGAAGCTGGGC1110.11308990137745539No Hit
AATAGTGGGATATTTGAGTGGGCGGTGGCGATTGGTGTTGGCGGTCTGGCTC1110.11308990137745539No Hit
ACATATGGGGTTCCGGGGCCGCATGTCCATGAAGGAGGTGGACGAGCAGATG1110.11308990137745539No Hit
TAACACGGGGCAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATC1100.11207107343711793No Hit
AGTATGGGGGTACCTTCACTCATCAGGGTCTACACTTTCTGCATAAAATTTT1100.11207107343711793No Hit
CTAGGGTTGGGAACCTCAGCCACTTTTCATTTTTAAAATTCTAACCTGTATA1100.11207107343711793No Hit
ACGCAGGGGCATGGGATGTGGGTTGTCCTTGGAAGGGTCAACCTTGGGTGAA1090.1110522454967805No Hit
CGCACAGGGGACCAAGATGGCGGCCGTGGGGCGAGTCGGCTCCTTCGGTTCT1090.1110522454967805No Hit
GCACCTGAGTGAGACATCCTTCCTTCCAAGAATCCCTGCCCCAGGTCAGTGT1090.1110522454967805No Hit
AGTTGAGGGCAGACAGAAGATCTGAGATCCAAGAAGGACTGGGAAGCAAAGA1090.1110522454967805No Hit
GATACTGGGAGGCCACTCTTTTTCTACCAGTTGTTTTATGAATCCACCTATT1090.1110522454967805No Hit
GTCTCAGAAATACCCAGCACCCAACAAGTTAAGATTCACAATGTCTAGCATT1080.11003341755644308No Hit
GGTACAGGGGGAGGAGGGAGGGAGAAAGGCAGAGGAGGAGGAGACAAATTTT1080.11003341755644308No Hit
TACGCTTGGGGAAAGGATATAGTTTTACGTAACATTTGGGGAAAAACAGTTA1080.11003341755644308No Hit
AAAAGTGGGAAGAAAAATATTGCCATCAAGAATTACTTGTGTTTTCACAGAG1070.10901458961610562No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTA1070.10901458961610562No Hit
CTTGACGGGAAGATGAAATAATAGATAAAAGCTATACTCCATCTAAAATAAG1050.10697693373543077No Hit
TAAAAGAGGGATGATCCGGGCCCAGGAACAGAAACGAGATCTCCATGATCAA1050.10697693373543077No Hit
GTGAAAGGGTCCTCAATGTTGGTTTAAGAGTTGTCTAAACCAGTTTCTTGTT1050.10697693373543077No Hit
CCGCGCGGGGGTTTGTGAAAGCAATTTTATCACCAAAAATTAAATGACAGTC1050.10697693373543077No Hit
GTGACTGGGCAGATGCCTGAGGGGATGGTTGAGGCCCAGGCAGCTCCTGCAT1040.10595810579509334No Hit
ATGTAAGGGTGAAAGAAGAACGTCCAGAAAAAATACCAGATTTAAAATTATT1040.10595810579509334No Hit
GAAAGGGGTCTTGATAATTTACTGTATATATTACTTATGTTTCTGAGAAATT1030.10493927785475587No Hit
GTATACGGGTCCAAATAATTTTTTCTTATCCCATAACTTTTAATACATCTAC1030.10493927785475587No Hit
GTAATAGGGAGCACTGAACTGATCCACTCTGATTTTCATTTATTTATTAAGT1030.10493927785475587No Hit
ATAGTTGGGAAGATGCTCACTCCTGAGCATGTGTTTATTCATCCGGGATGGA1030.10493927785475587No Hit
ATAGTGGGATTGGAAGATACGATTTTCAGCAAGTCCTAAAATGCTTATTTGC1020.10392044991441846No Hit
ACATTAGGGCGATCTTTACTGGGGATTCGGAGCTGCGGGAGCCGGGCTGGCA1020.10392044991441846No Hit
ATTTCAGGGCGATCTGCTACGGGGATGACCCCAATACGCAAAACTAACCCCC1020.10392044991441846No Hit
ACTATTGGGAAGTTTGATCACCAGGACTGTTTAGTTTCCCTTAAAATACTTT1020.10392044991441846No Hit
GTCAAAGGGATTCTGCATAGGGTGAGATGAAACTTCAGGATTTTTTATGCCC1020.10392044991441846No Hit
AGAAATGGGGGGGCTCCGCCTGCAGTGTGGCCCGTCCGGACAGTCCCTCACC1020.10392044991441846No Hit
GCTAATGGGGTAATAATAGGTTAATATTCAATCTATATAAAGTACTCAACTC1010.10290162197408102No Hit
TAACAAGGGGAAGATGAAAATGGACAAAGGTGGCTCTGTACTGGGGATATTG1010.10290162197408102No Hit
ATGCCTGGAATGCATATTAAGAAATTTTTAATGTTTTCTATAATTTTATAGT1010.10290162197408102No Hit
ATTTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1010.10290162197408102No Hit
CATAGTGGGATCTCAGTATTTAATTTTTATTCACTTTTTCCATTCCTTTAAG1010.10290162197408102No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCCTGTCTCT1010.10290162197408102No Hit
GTAATAGGGTTAACAACCCATTAATATTAACGTGACTTTAGCACTAATAAGA1010.10290162197408102No Hit
TCTTGAGGGGTATCCCAACTCTATGACACAGTGAGAATAACATGTAATAACA1010.10290162197408102No Hit
AATTGGGCATTTGAATATTCTCTTTGGTGTAGTTTATGTTCAAGACTTTTCT1010.10290162197408102No Hit
TGCTTCGGGAAGGGTTTTGATGAAAGATATTGTTACCCCAGTGCCACAAGAG1000.10188279403374359No Hit
GGGTCACGGGTGATGCTTCCCCTGTATTTTCCCTTTTCTTTAACCTTTAGGA1000.10188279403374359No Hit
AAATTGGGGTTAGTGATAGATACAATATGGGTCCTATTAGCAGGCGTTTCAT1000.10188279403374359No Hit
CCTACTGGGAAGGAGATTGCGAAGATGGCAGAGATGCTGGTCGAGCTCGTCT1000.10188279403374359No Hit
CGCCTGGGCGACAGAGCCAGACACTGTCTTGAAAGAGAAATAAAAGTTGCTT990.10086396609340614No Hit
TATACCTCATATAAGGAGAAGAGCATCTTTCTCGTGGCCCACAGGAAAGATC990.10086396609340614No Hit
ATTGCAGGGTGGTCCATACAGTCAGCGTCCCCAGGCAATGCTCAGAGTCTCT990.10086396609340614No Hit
TGTACAGGGAACAAAGCCCAACTATATACTCTCTACAGGACACATTTTACAT990.10086396609340614No Hit
TGCTTAGGGGATCTCTTTAGCGGGATATTATTCTCCAAGACTTTACCTATGT990.10086396609340614No Hit
TTTTCTGCGGGAGTATTTCAAGTTCTGGTCTTTTGTAGCATCTTTTTTATTC990.10086396609340614No Hit
GCTCGGGGTTACAGTGGCCTGCTATTGAGGAAAGGTATTCTTCCATACAACT990.10086396609340614No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)