FastQCFastQC Report
Sat 31 Dec 2022
SRR5848692.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848692.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97258
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT7840.8061033539657406RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC7830.805075160912213No Hit
AGCAATGGGTCAGGCTCGACTTCTAATTCTAGTTCTCTTGTTATTTCTACCA1720.17684920520676964No Hit
GGTGAAGGGTGAAGTTAACAACGGACTTGGGAAATCTCAGGCTAAGAACAAC1320.1357214830656604No Hit
CAATCTGGGCAATTTACAAAGAGAAGTTCCTGATGAAAGAAGTGTATCTTGA1300.13366509695860496No Hit
GAAACAGGGATGAATTTAAAGTTTATTATCCCAATTCTACAGAAGGATTTAA1280.1316087108515495No Hit
CAATTAGGGGAACAATGGGGGTTTCAAAACATAAAAGTGAAAGTCCTTGTGA1280.1316087108515495No Hit
TAACACGGGGCAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATC1270.13058051779802177No Hit
CAGAACGGGGAGGATTAAAACGCCAAAAGCAATGGCAACAAAAGCCAAAGTT1260.12955232474449402No Hit
TGTACAGGGAACAAAGCCCAACTATATACTCTCTACAGGACACATTTTACAT1260.12955232474449402No Hit
GAAATGGGGGCACCTGACTGTCTTCTGCATCTTCTGTGGTGGCTGCTTCTAG1250.1285241316909663No Hit
GCATTAGCTTAAATGTTTATTGCTTTGTAAATCACACAATACTCTGTTATTT1250.1285241316909663No Hit
TGTATGGGGCTGCTTGGCCTCTCAGTCTTAAAAATCAACTTTTGATTCTCTT1240.12749593863743855No Hit
TTAGCTGGGCAACTTCATGTACCAGCGTAAAGATTAAAGTGGAAAACTTCAG1240.12749593863743855No Hit
TTGCACTGGGATTTTTTAAATATATAAAAACCTAAAAAGGAATTTGTGACAT1220.1254395525303831No Hit
CGCCTGGGCGACAGAGCCAGACACTGTCTTGAAAGAGAAATAAAAGTTGCTT1200.12338316642332764No Hit
AATGCTGGGGATGTATTTGTTGTAGCAGCCTGAGCTAAGACAGACATATATG1200.12338316642332764No Hit
AGTTATGGGTGCCATAAATTATGTGTCTTCTGCATTGTTAAAATAAATTTAT1190.1223549733697999No Hit
CTAGGGTTGGGAACCTCAGCCACTTTTCATTTTTAAAATTCTAACCTGTATA1180.12132678031627217No Hit
TTGTTCGGGCAGAGGTGGGATGCAGATCTTCGTGAAGACCCTGACTGGTAAG1180.12132678031627217No Hit
GTGTTTGGGGGTCCAGCTGTGGTTGATAAGAGGTATATCTCTTAAAAAAGAC1180.12132678031627217No Hit
GGTACAGGGGGAGGAGGGAGGGAGAAAGGCAGAGGAGGAGGAGACAAATTTT1170.12029858726274445No Hit
AGGTATGGGAACTGTTTCAGGGCTCTAGAAATGGAATAAATGCAAGAAAGAA1170.12029858726274445No Hit
AGAAAAGGGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCT1170.12029858726274445No Hit
AGCTTTGGGGGGCTGTGGCAGTGGACCTGGATGGCAGGCAGAGCAGAGGACC1160.11927039420921672No Hit
CACTTGGGGCAGCAGCAGTGAGGATGAAGATGAGACCCAGCATCAAGATGAA1150.11824220115568901No Hit
AGTTAAGGGCAAGGACCTGAGCGGGTGCTGAGACCAGGTGCTCCAGAGGCCC1140.11721400810216127No Hit
CATAGTGGGATCTCAGTATTTAATTTTTATTCACTTTTTCCATTCCTTTAAG1140.11721400810216127No Hit
TCGTATGGGTGCAAGGCACTGAGGAGTATCAAAGAAGCAAATATTTTCACTT1130.11618581504863354No Hit
ACTATTGGGAAGTTTGATCACCAGGACTGTTTAGTTTCCCTTAAAATACTTT1130.11618581504863354No Hit
TATCACGGGTGGACAGGTAAGTTTGAGATGCCTGGTAGTTATCCCAGGGGAA1120.1151576219951058No Hit
AGTACGGGGATGGAAAATGTTGTCCTGTACTTACTAGGCAAAACGTATGAAT1120.1151576219951058No Hit
CTTGCCGGGAGGAGGCCGGAGTACACGCCGATCCACCTCAGCTGAACTGGCA1120.1151576219951058No Hit
AGTTGAGGGCAGACAGAAGATCTGAGATCCAAGAAGGACTGGGAAGCAAAGA1120.1151576219951058No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTA1120.1151576219951058No Hit
TAGTTAGGGCATATCATCTGTTATGTCATCTGATGTCTGTGCTTTCTCTCTA1110.11412942894157808No Hit
ATGCTAGGGAAACAAGTTGAATATGTTTTCATACATTCATACTATAAACTGT1110.11412942894157808No Hit
GTTGTTGGGGTCTTCCACACGGTTGAACTAATTTACATTCCCACCAACAATG1110.11412942894157808No Hit
CCTACTGGGAAGGAGATTGCGAAGATGGCAGAGATGCTGGTCGAGCTCGTCT1100.11310123588805035No Hit
GGCAATGGGCACTGAATAAAAACATAATCCATGAGTTCATGCTGATACTCAA1090.11207304283452262No Hit
AGCGTGGGGTATGGACGATAGCATCATAAGCCTTTTCTTCTAGATGTTTTAA1090.11207304283452262No Hit
CAAAGAGGGGATCATCCAGCGGGAGGTATTCACTATGAAATGTGCATAGAAT1090.11207304283452262No Hit
AATCTAGGGCATCCACCAGAGCCTCGCGGCCACCATGTTTGAATTTGCCTTT1090.11207304283452262No Hit
CAGCCTGGGCAACATAGCAAGACCCTGTCTCTACAAAAAATAAAATGTAAAA1090.11207304283452262No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC1080.11104484978099488No Hit
TGTGCGGGTAAGTGAACTATTTCAGGGCTATCAAATTGTTATAATTTGGGAT1080.11104484978099488No Hit
TATGTGGGGGATTTGTAAAGCTGCTTTTAGCTTTAAGTTTCTGTAAGTTACA1080.11104484978099488No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCCTGTCTCT1080.11104484978099488No Hit
ATTGCAGGGTGGTCCATACAGTCAGCGTCCCCAGGCAATGCTCAGAGTCTCT1070.11001665672746716No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGA1070.11001665672746716No Hit
GTTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.11001665672746716No Hit
GGGTCACGGGTGATGCTTCCCCTGTATTTTCCCTTTTCTTTAACCTTTAGGA1070.11001665672746716No Hit
TGCTTCGGGAAGGGTTTTGATGAAAGATATTGTTACCCCAGTGCCACAAGAG1060.10898846367393943No Hit
CTTGACGGGAAGATGAAATAATAGATAAAAGCTATACTCCATCTAAAATAAG1060.10898846367393943No Hit
CATTTTGGGTAGCAGAAGTGATATTAAATATGTGCCCTCTCCTTGTATTCCA1050.10796027062041169No Hit
GTGAAAGGGTCCTCAATGTTGGTTTAAGAGTTGTCTAAACCAGTTTCTTGTT1050.10796027062041169No Hit
GCACCGGGATGATGACTAGAGACTATTTGTCAGTCAAAATTTGGGACTTAAA1050.10796027062041169No Hit
GATACTGGGAGGCCACTCTTTTTCTACCAGTTGTTTTATGAATCCACCTATT1050.10796027062041169No Hit
AGTATGGGGGTACCTTCACTCATCAGGGTCTACACTTTCTGCATAAAATTTT1040.10693207756688396No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCCTGTCTCTTATAC1030.10590388451335624No Hit
AATAGTGGGAGCTTGTATTAGATTCACATATTTACTCAGTAAGAATTCTTTC1030.10590388451335624No Hit
GTGATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1030.10590388451335624No Hit
ACATATGGGGTTCCGGGGCCGCATGTCCATGAAGGAGGTGGACGAGCAGATG1030.10590388451335624No Hit
ATGCAAGGGATGGCCCGCGGGAAAGCCAAGGAGGAGGGCAGCTGGAAGAAAT1020.1048756914598285No Hit
TGCTTAGGGTATTCTAGGCATTCGAGTCTCTTCCCAAATACCACCTTCTCAA1020.1048756914598285No Hit
ATTTCAGGGCGATCTGCTACGGGGATGACCCCAATACGCAAAACTAACCCCC1020.1048756914598285No Hit
GATCTTGGGGAAGCAAGAATCCCAGGATGCTAATTCTGTCCAATGAGTCACT1020.1048756914598285No Hit
CGTAGGGGAAAGAAAAGAGCAAGCATCTCAAAACCAAAACATGTAAAAAAGT1020.1048756914598285No Hit
TGCAATGGGGAGTGCTACCAATGAAAATAACACCAATCCTGAGCCTCAGTTC1010.10384749840630077No Hit
TTGCTGGGGAGGATCTCAAAGAGACATTTTCCCTCTCATATTCATTGAAACA1010.10384749840630077No Hit
ATAGTGGGATTGGAAGATACGATTTTCAGCAAGTCCTAAAATGCTTATTTGC1010.10384749840630077No Hit
TGCTTAGGGGATCTCTTTAGCGGGATATTATTCTCCAAGACTTTACCTATGT1000.10281930535277303No Hit
GCTCGGGGTTACAGTGGCCTGCTATTGAGGAAAGGTATTCTTCCATACAACT1000.10281930535277303No Hit
CGCACAGGGGACCAAGATGGCGGCCGTGGGGCGAGTCGGCTCCTTCGGTTCT1000.10281930535277303No Hit
CCGCGCGGGGGTTTGTGAAAGCAATTTTATCACCAAAAATTAAATGACAGTC1000.10281930535277303No Hit
GTCATGGGGAAGCAATGCAAATGTCTAATTGTCCCCCAGTGGGCATAGTCAG1000.10281930535277303No Hit
AATAGTGGGATATTTGAGTGGGCGGTGGCGATTGGTGTTGGCGGTCTGGCTC1000.10281930535277303No Hit
GATCATGGGACTGTCACTATTTGTTATAAAGCTCAAGGTTAGTTTTCATTGA1000.10281930535277303No Hit
GTTGCGGGAAGATACCAGATTCAGGTTGGCTGAATTCTAGTTCTAGTTTTTC990.10179111229924531No Hit
ACGTATGGGACCCTCTCTGGCCTTAGTTTTCTTGTTTGCTATCAAGCTTGGG990.10179111229924531No Hit
ATTGCAGGGCAGAGGAGAGAGACATTCAGACAGAGGAAAGTCTCCCTGCCCC990.10179111229924531No Hit
AAAAGTGGGGATTCAGATCAATGAAAATATAAAAATTACTTATTGGTTTTCC990.10179111229924531No Hit
GCACATGGGTATGTCCAAAGACCCTTTATTCGAATGTCCTGGGAGAAGGAAG980.10076291924571758No Hit
CGTCTTGGGTGGTTTCTCCTTTGATTGCTGGTAAATTCTGAGGCCACAGAGA980.10076291924571758No Hit
AATTAAGGGTGCCTATGGCTTTATCAATCTAGTCTTTCAGCCATTGTTTATT980.10076291924571758No Hit
TTTTCTGCGGGAGTATTTCAAGTTCTGGTCTTTTGTAGCATCTTTTTTATTC980.10076291924571758No Hit
GCACCTGAGTGAGACATCCTTCCTTCCAAGAATCCCTGCCCCAGGTCAGTGT980.10076291924571758No Hit
AGCTCAGGGCAAAATCTTGGGGGAGGGAGAGAGTTGTAAAAAACCTAGATCC980.10076291924571758No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)