FastQCFastQC Report
Sat 31 Dec 2022
SRR5848691.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848691.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96939
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT8110.8366085889064256RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC6790.7004404831904599No Hit
AGCAATGGGTCAGGCTCGACTTCTAATTCTAGTTCTCTTGTTATTTCTACCA1800.18568378052177142No Hit
TTGCACTGGGATTTTTTAAATATATAAAAACCTAAAAAGGAATTTGTGACAT1360.14029441194978284No Hit
ATAGTGGGATTGGAAGATACGATTTTCAGCAAGTCCTAAAATGCTTATTTGC1330.13719968227442No Hit
GAAACAGGGATGAATTTAAAGTTTATTATCCCAATTCTACAGAAGGATTTAA1310.13513652915751143No Hit
TGTACAGGGAACAAAGCCCAACTATATACTCTCTACAGGACACATTTTACAT1260.12997864636523998No Hit
CATAGTGGGATCTCAGTATTTAATTTTTATTCACTTTTTCCATTCCTTTAAG1240.12791549324833143No Hit
AATGCTGGGGATGTATTTGTTGTAGCAGCCTGAGCTAAGACAGACATATATG1220.12585234013142285No Hit
ACGCAGGGGCATGGGATGTGGGTTGTCCTTGGAAGGGTCAACCTTGGGTGAA1210.12482076357296858No Hit
AATTGCGGGTCCTGGGCACTGAGGTCTGTTAGATGAAAGGATATGAGAAAGG1210.12482076357296858No Hit
ATTTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1180.12172603389760571No Hit
GCATTAGCTTAAATGTTTATTGCTTTGTAAATCACACAATACTCTGTTATTT1170.12069445733915143No Hit
TAGTTAGGGCATATCATCTGTTATGTCATCTGATGTCTGTGCTTTCTCTCTA1160.11966288078069715No Hit
GTTGTTGGGGTCTTCCACACGGTTGAACTAATTTACATTCCCACCAACAATG1160.11966288078069715No Hit
CCTACTGGGAAGGAGATTGCGAAGATGGCAGAGATGCTGGTCGAGCTCGTCT1160.11966288078069715No Hit
CCAGCAGGGTCCCTATTGAAGGAAAAATGAGTAACTATCAATTAAACACATA1150.11863130422224286No Hit
CGCACAGGGGACCAAGATGGCGGCCGTGGGGCGAGTCGGCTCCTTCGGTTCT1150.11863130422224286No Hit
AAAAGTGGGGATTCAGATCAATGAAAATATAAAAATTACTTATTGGTTTTCC1150.11863130422224286No Hit
CAATTAGGGGAACAATGGGGGTTTCAAAACATAAAAGTGAAAGTCCTTGTGA1150.11863130422224286No Hit
GGTGAAGGGTGAAGTTAACAACGGACTTGGGAAATCTCAGGCTAAGAACAAC1120.11553657454688No Hit
AATAGTGGGAGCTTGTATTAGATTCACATATTTACTCAGTAAGAATTCTTTC1120.11553657454688No Hit
TCTTGAGGGGTATCCCAACTCTATGACACAGTGAGAATAACATGTAATAACA1100.11347342142997144No Hit
TCGTATGGGTGCAAGGCACTGAGGAGTATCAAAGAAGCAAATATTTTCACTT1090.11244184487151715No Hit
AGTTAAGGGCAAGGACCTGAGCGGGTGCTGAGACCAGGTGCTCCAGAGGCCC1090.11244184487151715No Hit
AGGTCGGGACCTGAAAGCTGCAAAAATTAATGGTTTAGATATATTTATAATA1090.11244184487151715No Hit
GCACCGGGATGATGACTAGAGACTATTTGTCAGTCAAAATTTGGGACTTAAA1080.11141026831306285No Hit
AATCTAGGGCATCCACCAGAGCCTCGCGGCCACCATGTTTGAATTTGCCTTT1080.11141026831306285No Hit
CAGAACGGGGAGGATTAAAACGCCAAAAGCAATGGCAACAAAAGCCAAAGTT1070.11037869175460856No Hit
AATAGTGGGATATTTGAGTGGGCGGTGGCGATTGGTGTTGGCGGTCTGGCTC1070.11037869175460856No Hit
AGTTGAGGGCAGACAGAAGATCTGAGATCCAAGAAGGACTGGGAAGCAAAGA1070.11037869175460856No Hit
GGGTCACGGGTGATGCTTCCCCTGTATTTTCCCTTTTCTTTAACCTTTAGGA1060.1093471151961543No Hit
CACTTGGGGCAGCAGCAGTGAGGATGAAGATGAGACCCAGCATCAAGATGAA1060.1093471151961543No Hit
CTTCACGGGATCTTGTCGTTCATTTGTATTAATATTTGGATATTGTGAGTTA1050.10831553863769999No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC1050.10831553863769999No Hit
TTATGAGGGAGCATTTCATTACCTGGGTTTGTTCTAAAGAAGATTTATTTTT1050.10831553863769999No Hit
GTGACTGGGGAGACTTCCTGAGCAGAAATGGGCGAACTTCAGTCTTTTTACT1050.10831553863769999No Hit
AGCTCAGGGCAAAATCTTGGGGGAGGGAGAGAGTTGTAAAAAACCTAGATCC1050.10831553863769999No Hit
GTCAAAGGGATTCTGCATAGGGTGAGATGAAACTTCAGGATTTTTTATGCCC1050.10831553863769999No Hit
CTAGGGTTGGGAACCTCAGCCACTTTTCATTTTTAAAATTCTAACCTGTATA1050.10831553863769999No Hit
TGCTAGGGAGATCATTATACTTGGAACTTCTAAATCATGCAATTTCTGAATA1050.10831553863769999No Hit
TTAGCTGGGCAACTTCATGTACCAGCGTAAAGATTAAAGTGGAAAACTTCAG1050.10831553863769999No Hit
AGTGCAGGGATAAAGACCAATGAGCATGCTAGAGAATTTCTGCTTCAGGTAG1040.1072839620792457No Hit
CAATCTGGGCAATTTACAAAGAGAAGTTCCTGATGAAAGAAGTGTATCTTGA1040.1072839620792457No Hit
TACAGCGGGAACACAACAGATTATCAGAGCCAATTAGTCAGTGATGGAAACA1030.10625238552079141No Hit
GAAATGGGGGCACCTGACTGTCTTCTGCATCTTCTGTGGTGGCTGCTTCTAG1030.10625238552079141No Hit
AGCACTGGGCGCTCTTCCGATCTGTCTAAGGGAACTAACCAGTCCTACATAT1030.10625238552079141No Hit
ATGCAAGGGATGGCCCGCGGGAAAGCCAAGGAGGAGGGCAGCTGGAAGAAAT1020.10522080896233714No Hit
GTATCGGGGAATGTATTATCTTGTTTTCTTTTCATCATGTTTTCGTTTATTT1020.10522080896233714No Hit
ATGCCTGGAATGCATATTAAGAAATTTTTAATGTTTTCTATAATTTTATAGT1020.10522080896233714No Hit
CTTGACGGGAAGATGAAATAATAGATAAAAGCTATACTCCATCTAAAATAAG1020.10522080896233714No Hit
GCACCTGAGTGAGACATCCTTCCTTCCAAGAATCCCTGCCCCAGGTCAGTGT1020.10522080896233714No Hit
TCGTGCGGGGGTTTCCAGCTTCATCCATGTCCCTAGTAAGGACATGAACTCA1010.10418923240388285No Hit
CTACTGGGGGTAGCTGGAGTAGTTGGGTTGCTTTGTGTTAGGAGGATCCAGA1010.10418923240388285No Hit
GTTTCAGGGAATTATTTTTATGGTTTATTGAATTAAAACCCTAGTAAAGTTT1010.10418923240388285No Hit
TAGGGGGAACAAGATGAGATTGGCATGGCTTTATTTGTTTTTTTTGTTTTTT1010.10418923240388285No Hit
TAACAAGGGGAAGATGAAAATGGACAAAGGTGGCTCTGTACTGGGGATATTG1000.10315765584542856No Hit
CCTCTTGGGGAGCTACTTGTAGATGAAGAAGAAGCAGCAGCACTGCTAGAAA1000.10315765584542856No Hit
ATCAAGGGGATAAAAAAGAAATTGAAGGTGAAAAGGAAAAATACAAAACTAA1000.10315765584542856No Hit
ATTTCAGGGCGATCTGCTACGGGGATGACCCCAATACGCAAAACTAACCCCC1000.10315765584542856No Hit
TACTAAGGGAAGAGCTACCTGGACAACTGAAACAATTCTCCATGTTTCTGGG1000.10315765584542856No Hit
TATGTGGGGGATTTGTAAAGCTGCTTTTAGCTTTAAGTTTCTGTAAGTTACA1000.10315765584542856No Hit
CCTTAAAAATCTCTGTAACCATTTCTTTTATGTACATTTGAAAATGCCCTTT1000.10315765584542856No Hit
TTTACCGGGATGCCTCCTTCTTTAGATTGTCGATATAATAGAGAAGGGGAAC1000.10315765584542856No Hit
AAATTGGGGTTAGTGATAGATACAATATGGGTCCTATTAGCAGGCGTTTCAT1000.10315765584542856No Hit
TGCTTAGGGTATTCTAGGCATTCGAGTCTCTTCCCAAATACCACCTTCTCAA990.10212607928697429No Hit
GTCATGGGGATACTTAGCTATTTTTGTTAAAGCAGGTGATTTATTGTTATTT990.10212607928697429No Hit
GTAGTCGGGCATGGTGGCCCCACTATAGAATGCATTCTCTTTTTTGGGGTCC990.10212607928697429No Hit
AGAAAAGGGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCT990.10212607928697429No Hit
GCTCGGGGTTACAGTGGCCTGCTATTGAGGAAAGGTATTCTTCCATACAACT990.10212607928697429No Hit
GGTACAGGGGGAGGAGGGAGGGAGAAAGGCAGAGGAGGAGGAGACAAATTTT980.10109450272852No Hit
GCACATGGGTATGTCCAAAGACCCTTTATTCGAATGTCCTGGGAGAAGGAAG980.10109450272852No Hit
TAGAACGGGATTTGAAGTATGACTTTTATTGAACGTGATTCACTTTTACACC980.10109450272852No Hit
AGAATCGGGCTAAGAGTTATTGGCCTTAAAGAGGAGGTAAAGAAAGAGATAG980.10109450272852No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTA980.10109450272852No Hit
TGCTCCGGTCTGGGGGATTTGGTTGTGAAGATTCGCTGAATCACTTGTTGAA970.10006292617006571No Hit
AGTATGGGGGTACCTTCACTCATCAGGGTCTACACTTTCTGCATAAAATTTT970.10006292617006571No Hit
CATTTTGGGTAGCAGAAGTGATATTAAATATGTGCCCTCTCCTTGTATTCCA970.10006292617006571No Hit
ATGCTAGGGAAACAAGTTGAATATGTTTTCATACATTCATACTATAAACTGT970.10006292617006571No Hit
CGTAGGGGAAAGAAAAGAGCAAGCATCTCAAAACCAAAACATGTAAAAAAGT970.10006292617006571No Hit
TGCACAGGGACATGAATGATCACAGACCTAAGGCCCAGGCCACCTCTCCAGA970.10006292617006571No Hit
TCTCAAGGGCTATAAGTTACATAAATGTAGGACTCTGTTTTGTTCACAGTAT970.10006292617006571No Hit
TGCGAAGGCGATCTAAGCTCGGGACTTCCACATTCTGGCTATAATTTCCTTT970.10006292617006571No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)