FastQCFastQC Report
Sat 31 Dec 2022
SRR5848690.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848690.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102421
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT6200.6053446070630046RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC5460.5330937991232266No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA2250.2196815106277033No Hit
TCATCGGGGGGAAATAATGGCTGAAAACTTATCAAATTTAGCAAAACAACAT1490.14547797814901242No Hit
ATAAATGGGACACCTGGATGGGGTGCATGTAGTCTTTGGACTGGTTATTTCT1460.14254889134064302No Hit
TTGTTTGGGATATATCAATAATAACTTTGAATGTAAATGGCCTAAATCCCTC1400.1366907177239043No Hit
TTGACTGGGAAGTGCAGAAATCTTCAGTAAAATACCAGCAAACTGAATTCAA1360.13278526864607845No Hit
CCGATGGGGATTGGCATTTTAGACTTTAAAAATGCTGTATGATTTCAGATGA1360.13278526864607845No Hit
ACCTTAGGGGATCTTTAGTCGGGCTCCCTAAAGCCCATGTCGAAGCCCCCAT1360.13278526864607845No Hit
GTACTGGGGGGATTAAGAATCTCACTCAAAACTGCACAATTACATGAAAACT1340.13083254410716552No Hit
TTGTATGGGGGGGTAAGAAGCCTGCTGAGGGATGCCCAAGAAGTTCCAGGGT1330.12985618183770906No Hit
ACAGGTGGGCTTTCCGAAATGACTTTGCACAACCACAGCCAATGAAAACATT1320.1288798195682526No Hit
ATAGCAGGGGTCAGGGAGGAGGAGTAGAATTATGTGAGCTTACTTCAGAGCA1310.12790345729879615No Hit
TTAAGCGGGCAATTAATTGCTGTAAGAGACACTTCTAAATGATTTGGTCCCA1290.12595073275988322No Hit
CATTGTGGGGATGATCTCTGTGAACATGGAAGGTGTGTTTGCTTTAAGTTAG1270.12399800822097032No Hit
GCGCAAGGGACTAGTTGGTAATGTGATTATGTGGTACCTTGGCTTTAGGTTT1270.12399800822097032No Hit
TGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1260.12302164595151385No Hit
GGTGCTGGGGGAAGAGGACAAGCCTGAAAACATAAAGAAGTAAATGGCTAAA1230.12009255914314447No Hit
CATACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1230.12009255914314447No Hit
CTGTACGGGATTGTTGGGACAGGTGCCATTTAAATTGCCTCCATGCTCCCCA1230.12009255914314447No Hit
CTTTCAGGGCCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGACC1220.119116196873688No Hit
AGACTAGGGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGA1220.119116196873688No Hit
AAGAAAGGGCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1220.119116196873688No Hit
TCACATGGGTCAGGGCCCAGTGCACTCACTTCACAGTGACAGCCCTCCCCTA1220.119116196873688No Hit
ACGTCGGGGTCTTCAGTCAGATGTGAATGAATGTTAGCACATAACCCCTCCA1210.11813983460423157No Hit
CATAATGGGTGGCACTTCTCTGTTTGATGAAGAGGGAGCCAAGATTGTCAAA1210.11813983460423157No Hit
GCTATAGGGGTCACTAGTAAGAATGGACCCAATTCAACTTTTATCTTAAGTT1210.11813983460423157No Hit
CCAAGGGGAATATGAAGATGTATCTTTCTACTTGTAAGGGCCAGTAGCAAAC1210.11813983460423157No Hit
CTTGTAGGGATTCATCTTTTGGAGGGTGGGGGGAAAACCCAAAAACAACAAC1200.1171634723347751No Hit
CAAACGGGGATGTGTAGCACAGAACTGCACTAGGATTAATTTGTTTACAAGA1200.1171634723347751No Hit
AAGAGTGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT1200.1171634723347751No Hit
TGTTGAGGGCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAA1200.1171634723347751No Hit
TAAAACGGGTAGAGTGAAATACCTGATTCAGCTGGCTCCTCACTGTCTTGAT1200.1171634723347751No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC1190.11618711006531864No Hit
ATGCTTGGGAGAGAGTGCTTATGTTTTGTTTACTCATGTTTCAACTTGCTGT1190.11618711006531864No Hit
TCTAGCGGGGCGAGCACTTTTAATAAAATAATATGTACCATATTGGTATTGT1180.11521074779586218No Hit
CTGCGGGATGGTGCTAAGGTGGTTTTCTAGGTAACTGCAGGGATGGAGGTCA1180.11521074779586218No Hit
CTAGTGGGGACACCGAATCTTGATGAAAAAAGAGAAGAGCATTTGCCTTGTT1180.11521074779586218No Hit
AAGCCTGGGGTACTGCATTTATGCAATTAGCAAATAACTGCTAGTTTTCATT1180.11521074779586218No Hit
GTTTGGGGGGCTGAAAACAATCAGCAATGCCATACTACTGACACAAACGTTT1180.11521074779586218No Hit
GGTAGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA1180.11521074779586218No Hit
TTAATCGGGGATGTGAATCTTCCAATTTTATCTATTTGATTCAAATGGTATG1180.11521074779586218No Hit
CATTGTGGTACTCTCTTGTTTCCTTTAGATGGAACAACAAATTTTGGGGACT1170.11423438552640572No Hit
AAACTGGGGAATTGTAAGAATTGATTTTATGGAGCTTTTTGATATCTAATAA1170.11423438552640572No Hit
AACGGCGGCCGATCATAGGAGGGCTGATGTTCGCCGACCGTTGACTATTCTC1170.11423438552640572No Hit
CTTATTGGGGTTTTGAAAATGCTACTGAATTTGTCTGAAAATCTTTTCATGA1170.11423438552640572No Hit
TAAGAGGGATGACTACGAGAAGCACTTCACGGTCATGAACTATGACCCGGAT1170.11423438552640572No Hit
GTGCAATGGGAACACAGATACCTACAGACTCCGTTTTCATTTTCTCGTGTTC1170.11423438552640572No Hit
TGCAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1170.11423438552640572No Hit
AGCCACGGGGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC1160.11325802325694925No Hit
AGCACAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1160.11325802325694925No Hit
GAATAAGGGCGTCTCCTGTGTGTATGTGTGTGAGTGGTGGGCAGGCCAGTGG1140.11130529871803635No Hit
CAAACAGGGGAGAACACCAATGTAACATTTCTGAAACTAGTGAGTTGCAAAC1140.11130529871803635No Hit
ACGAACGGGGTTAATAATCAGAGAAATCATAACGAGATGGCTTCTAAAAATA1140.11130529871803635No Hit
TTTGCGGGGCAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGG1140.11130529871803635No Hit
GACTTCGGGCTCCGCTCGATCTGGCCAAGCTGAACCAGGTGGCAAGACAACA1130.11032893644857988No Hit
TGCTATGGGTCCCTTATATAAAATTGAAATGGTATCATGTTTGCATATAACA1130.11032893644857988No Hit
CTCGGACCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1130.11032893644857988No Hit
TTCAACGGGATGGCTGTAGATATGCAGATATGTCTTACACAGACAGAAAGTT1120.10935257417912343No Hit
GTTGATGGGTCATTCTCTACTGTCACAGTGGCTCCGGAAGCCTGGTTGGCAT1120.10935257417912343No Hit
GTCGATGGATGTAGAGTCTCTAATAATTGCTAAATAGCAATGTGAATGATTA1120.10935257417912343No Hit
ATGTTCGGGACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTA1110.10837621190966697No Hit
TATGCTGGGGAAGCGATGAGAAACTTATTTCTATGGGACAGAGTCTTTTTTT1110.10837621190966697No Hit
TTTACAGGGAGGCTGAGAAAGGTGAGGAAGCTACAGAAGAAAATTCTAAAGC1100.1073998496402105No Hit
GTCCTCGGGGCAGCAGCCACTTATCAAACCCCTACTTTAATATTTCACTCAA1100.1073998496402105No Hit
AAAGGGGATTGGGACAAGAAGGATCATCCCCGCTTCCATTTAACATTGTATT1100.1073998496402105No Hit
TCACATGGGAGACAAGCTGTGTGAATGCATAGATGTAATCTTGTCTCAAATA1100.1073998496402105No Hit
ACGCCGGGGATCAGCCAAAATTCAAGTATCAGATAACATAAAGATTTGTTTT1090.10642348737075404No Hit
AACCTAGGGGAAGTTGCATGGGGCCCTAATGAATGATGGGCAATTTGCTGTA1090.10642348737075404No Hit
CAAGCTGGGAGATGGATGTAGTGAATGCTTTCCAGAGTGGAAGTTCCATTCA1090.10642348737075404No Hit
AATACCGGGTGGACAGAAAAGTGAAAATGGTTGGTGATGTGATGGTGCCATG1080.10544712510129758No Hit
TTTTACGGGTAAATTGTCATATGTTGTTATTTTCAGGTCAAGAGCATCAACT1080.10544712510129758No Hit
GACGAAGGGCAGACTAAACAGAAGCAGACCATCCACCTGCAGGCAGACCAGC1080.10544712510129758No Hit
TCACTTGGGTTTTACTTTTTGTTTTCATGAGAATTTTGATAGTGCTACTCAT1070.10447076283184112No Hit
AACAAGGGGAAGCTCCAATCATCGGCCAGACAGTTGCTTTATGTAGGTTTTT1070.10447076283184112No Hit
TATTGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1070.10447076283184112No Hit
ACAGTGGGGGTATAAAATGTGTATTGGTTAGCATTTAGTACTAAGAAATTCT1070.10447076283184112No Hit
CAATCCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1070.10447076283184112No Hit
TAAGTCGGGGATAGACCTAATTTGCAAACTCAATCGGGGACTAAAATTTCCC1070.10447076283184112No Hit
AAAGTTGGGCGTGAACCAAACCTCTGCTAGTCTCAGACTTTTCTTCTGTAGC1070.10447076283184112No Hit
TACCGCGGGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTT1060.10349440056238468No Hit
GTACACGGGAGGAGGTACCGACAGTCCCTGTGCTGGTTAGACACGGAGCGCT1060.10349440056238468No Hit
GCATGTGGGCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTT1060.10349440056238468No Hit
CTATTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1060.10349440056238468No Hit
AATCTGGGGAAGACAAACGCTGAGCTGTGACCAATCCAGCTGTTCTCTGTAG1060.10349440056238468No Hit
GGGTCTGGGTACAACCACTCTAAAAAACCTTGGGGCACTTTACAGACGTCAA1060.10349440056238468No Hit
GCCATTGGGGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA1060.10349440056238468No Hit
ATTGCAGGGCTGGCGGTGGCGGAGGCGGCGGCCCCTCGCCTTGTGGCCCTGG1050.10251803829292822No Hit
TACTCGGGTATTCTCAATTCTTTTTTTTCCGCCCTTCCTTGGCAATGGCTGG1050.10251803829292822No Hit
ATGCCTGGGGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGA1050.10251803829292822No Hit
TCATTAGGGCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC1040.10154167602347175No Hit
GCCTGTGGCTGACAATACTTCACCTTTCTTTTAATTCCCCATGATGTTTTCA1040.10154167602347175No Hit
CAGTTAAGGGATTACATATTAATATAAATATGATTAATTCTAACAACTCCAT1040.10154167602347175No Hit
TGCGTTGGGGCAACAGGAAAATATCACAGTCCTAAATATATATGCACCTAAT1040.10154167602347175No Hit
AATTACGGGAAGAGAAGAGAGCAAAAGAGTGAAATTGTAGTTTTCTTCTATG1040.10154167602347175No Hit
AGCACTGGGGAATTACTAATATGATACAGTTTCTGATTCCAAAGTATAAGAA1040.10154167602347175No Hit
TGCTAGGGGATCTAAAGTTGGTTTAACATACACATATCAATAAATGTGATTC1030.10056531375401528No Hit
CTATGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1030.10056531375401528No Hit
AATGTTGGGTGACAAAATATACTTTAACAAAAATTGTTGCTAAAGACAAAAA1030.10056531375401528No Hit
TTGGACGGGGGAACCCAAGACTTTTGGTAGTTGGATTTGCTCTTTAAAGTTT1030.10056531375401528No Hit
ATCTAAGGGAAGTATTTGGGTGAGAACTGAGAGGTGGGAGATGATTGGAGGC1030.10056531375401528No Hit
CAATAAGGGGAGGACTGTGCAGAGATGAGGAAGACACCTCTTTTGAATCACT1030.10056531375401528No Hit
CAAACAGGGGAAGCAAGGTGGGTGGAAACTAAGACACCTGAACCCTCCAGGG1030.10056531375401528No Hit
TTTAGTGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG1030.10056531375401528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)