FastQCFastQC Report
Sat 31 Dec 2022
SRR5848689.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848689.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102760
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT6260.6091864538731024RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC5140.5001946282600234No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA1700.16543402101985208No Hit
CCGATGGGGATTGGCATTTTAGACTTTAAAAATGCTGTATGATTTCAGATGA1630.15862203191903465No Hit
GGTGCTGGGGGAAGAGGACAAGCCTGAAAACATAAAGAAGTAAATGGCTAAA1530.14889061891786687No Hit
GTCCTCGGGGCAGCAGCCACTTATCAAACCCCTACTTTAATATTTCACTCAA1490.14499805371739977No Hit
GCATAAGGGACTTTGCAGATCTCTTATGAATTCTTTCACTTATTTCTCATTG1440.1401323472168159No Hit
TCATCGGGGGGAAATAATGGCTGAAAACTTATCAAATTTAGCAAAACAACAT1430.1391592059166991No Hit
TTGACTGGGAAGTGCAGAAATCTTCAGTAAAATACCAGCAAACTGAATTCAA1380.1342934994161152No Hit
ATAGCAGGGGTCAGGGAGGAGGAGTAGAATTATGTGAGCTTACTTCAGAGCA1380.1342934994161152No Hit
GGGTCTGGGTACAACCACTCTAAAAAACCTTGGGGCACTTTACAGACGTCAA1360.13234721681588169No Hit
CATACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1330.12942779291553133No Hit
TAACGTGGGAGGATTTTGATGGAATGAGGAATCAACTTTATTGCGCTGAGCC1330.12942779291553133No Hit
GCGCAAGGGACTAGTTGGTAATGTGATTATGTGGTACCTTGGCTTTAGGTTT1310.12748151031529778No Hit
CTGCGGGATGGTGCTAAGGTGGTTTTCTAGGTAACTGCAGGGATGGAGGTCA1300.126508369015181No Hit
TATTGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1300.126508369015181No Hit
TCTAGCGGGGCGAGCACTTTTAATAAAATAATATGTACCATATTGGTATTGT1290.12553522771506423No Hit
GCTATAGGGGTCACTAGTAAGAATGGACCCAATTCAACTTTTATCTTAAGTT1290.12553522771506423No Hit
CAAACGGGGATGTGTAGCACAGAACTGCACTAGGATTAATTTGTTTACAAGA1290.12553522771506423No Hit
CTTGTAGGGATTCATCTTTTGGAGGGTGGGGGGAAAACCCAAAAACAACAAC1280.12456208641494744No Hit
CAAACAGGGGAGAACACCAATGTAACATTTCTGAAACTAGTGAGTTGCAAAC1260.1226158038147139No Hit
TGTTAAGGGCCTCCAAGTTTGCCCAGCATGCCTTCCGAACCTTCGACAAGAA1250.12164266251459713No Hit
GCATGTGGGCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTT1250.12164266251459713No Hit
ATACGCGGGCGGCGGCCCCAGCGGAGACTAGGCCAGAACTGAGCATTTTCAA1240.12066952121448035No Hit
CTAGTTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1240.12066952121448035No Hit
ACAGGTGGGCTTTCCGAAATGACTTTGCACAACCACAGCCAATGAAAACATT1240.12066952121448035No Hit
CAAGCTGGGAGATGGATGTAGTGAATGCTTTCCAGAGTGGAAGTTCCATTCA1230.11969637991436358No Hit
TATTAAGGGGTGTAATAAAACAATGGCACTTGCTTATAATGGTCTTTCAGTA1230.11969637991436358No Hit
AAGAAAGGGCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1220.11872323861424679No Hit
TTTGCGGGGCAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGG1220.11872323861424679No Hit
GTCTAGGGGGGCACCGGCCGCGCGGCGGGAGGAGGTGCCGGCCGAGCACCGC1210.11775009731413001No Hit
AACAAGGGGAAGCTCCAATCATCGGCCAGACAGTTGCTTTATGTAGGTTTTT1210.11775009731413001No Hit
GCATTGCGGGTGAGTGAAAGTTTCTAGAGTGTTTTCCTTGACTATTTTTGAC1210.11775009731413001No Hit
TTGTTTGGGATATATCAATAATAACTTTGAATGTAAATGGCCTAAATCCCTC1200.11677695601401324No Hit
CCAAGGGGAATATGAAGATGTATCTTTCTACTTGTAAGGGCCAGTAGCAAAC1200.11677695601401324No Hit
TGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1190.11580381471389646No Hit
GTACTGGGGGGATTAAGAATCTCACTCAAAACTGCACAATTACATGAAAACT1190.11580381471389646No Hit
ACGAACGGGGTTAATAATCAGAGAAATCATAACGAGATGGCTTCTAAAAATA1190.11580381471389646No Hit
CTATGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1180.11483067341377967No Hit
TTGGACGGGGGAACCCAAGACTTTTGGTAGTTGGATTTGCTCTTTAAAGTTT1180.11483067341377967No Hit
TCACATGGGTCAGGGCCCAGTGCACTCACTTCACAGTGACAGCCCTCCCCTA1180.11483067341377967No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC1170.1138575321136629No Hit
ATAAATGGGACACCTGGATGGGGTGCATGTAGTCTTTGGACTGGTTATTTCT1170.1138575321136629No Hit
CCATTAGGGGAGTTGCCTTAAAGAAAATTCAGAGCTGATATTCAGCCAGTAC1170.1138575321136629No Hit
TAAAACGGGTAGAGTGAAATACCTGATTCAGCTGGCTCCTCACTGTCTTGAT1170.1138575321136629No Hit
ATGCCTGGGGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGA1170.1138575321136629No Hit
AAGCCTGGGGTACTGCATTTATGCAATTAGCAAATAACTGCTAGTTTTCATT1160.11288439081354612No Hit
ATATAGGGGGCTTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCC1160.11288439081354612No Hit
CTGTACGGGATTGTTGGGACAGGTGCCATTTAAATTGCCTCCATGCTCCCCA1160.11288439081354612No Hit
GTAATAGGGGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAA1150.11191124951342936No Hit
CATAATGGGTGGCACTTCTCTGTTTGATGAAGAGGGAGCCAAGATTGTCAAA1150.11191124951342936No Hit
CTTTCAGGGCCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGACC1140.11093810821331258No Hit
CTCTGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1140.11093810821331258No Hit
GGTAGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA1140.11093810821331258No Hit
ATGCTTGGGAGAGAGTGCTTATGTTTTGTTTACTCATGTTTCAACTTGCTGT1140.11093810821331258No Hit
GAATAAGGGCGTCTCCTGTGTGTATGTGTGTGAGTGGTGGGCAGGCCAGTGG1130.10996496691319581No Hit
GTTGATGGGTCATTCTCTACTGTCACAGTGGCTCCGGAAGCCTGGTTGGCAT1130.10996496691319581No Hit
CTCGGACCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1130.10996496691319581No Hit
CGCTCTGGGAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTA1120.10899182561307902No Hit
AGACTAGGGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGA1120.10899182561307902No Hit
ATCTAAGGGAAGTATTTGGGTGAGAACTGAGAGGTGGGAGATGATTGGAGGC1120.10899182561307902No Hit
CTAGGTTGGGGCCACAATTTTCATGACTTTCTTCAGAAGTAGCACATTTTCG1120.10899182561307902No Hit
ACCTTAGGGGATCTTTAGTCGGGCTCCCTAAAGCCCATGTCGAAGCCCCCAT1120.10899182561307902No Hit
GAACTCGGGGTAAGACAAAGAGATAAAAGGCAGAAAGATTGGAAAGAAAGAA1120.10899182561307902No Hit
AGTCAAGGGTCTCAGAACCATATCTGTTGACATTTTTTCTCATAGAAATATC1120.10899182561307902No Hit
GTGCAATGGGAACACAGATACCTACAGACTCCGTTTTCATTTTCTCGTGTTC1110.10801868431296224No Hit
GTTTTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1110.10801868431296224No Hit
TGCAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1110.10801868431296224No Hit
TCGAAGGGGGGCAGTAGAGATTCAAGACTGTTTATGAATAAAGATGTCTATA1100.10704554301284547No Hit
GTTTGGGGGGCTGAAAACAATCAGCAATGCCATACTACTGACACAAACGTTT1100.10704554301284547No Hit
GAAACAGGGCTCTAAAGCCAATACTGGCCAATGCTTCACCAGTTAGTCATGC1100.10704554301284547No Hit
AAGAGTGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT1100.10704554301284547No Hit
CAATGTGGGATTGAAACAGCCAACAAGAATGAGTGAGTCACTCAGTGATATG1100.10704554301284547No Hit
AGTGTGGGGACACACCATAGATATTGCAGGAGGCAGCCACCATGTGAAATCT1100.10704554301284547No Hit
TGCTATGGGAAACATAGAAAAGTTAGAGTAAAAATAAAAGATAAAAGATAAA1090.10607240171272869No Hit
GATGCTGGGAAAAGGAAAAATAGTAGTAACTGCTCTGCTTTTTTACCATACT1090.10607240171272869No Hit
CTTATTGGGGTTTTGAAAATGCTACTGAATTTGTCTGAAAATCTTTTCATGA1080.1050992604126119No Hit
TCGACTGGGGCTTTGGGGCTTTGAAGGCAGGTTCAAGAAAGCATATGTCGAT1070.10412611911249513No Hit
GACTTCGGGCTCCGCTCGATCTGGCCAAGCTGAACCAGGTGGCAAGACAACA1070.10412611911249513No Hit
GAGACTGGGATATACCCTTGTCAATAAAAACATAGAATGTTCATTGCCAGTC1070.10412611911249513No Hit
CGTATCGGGGGATTAGGGATGCTCAACTTGCATCTAATACTCGGCTAGTATT1070.10412611911249513No Hit
AATCATGGGAGGACTCGGGAAGCAGTGCCAATGAACAAGCCGTGCAGTGAGA1070.10412611911249513No Hit
GAAGGCGGGATAGAAAGTAGCACCATCAACTGTGGAAAATGACCACATCCTT1060.10315297781237835No Hit
AACCTAGGGGAAGTTGCATGGGGCCCTAATGAATGATGGGCAATTTGCTGTA1060.10315297781237835No Hit
AAAGGGGATTGGGACAAGAAGGATCATCCCCGCTTCCATTTAACATTGTATT1060.10315297781237835No Hit
CTGCAAGGGGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGACGAGACA1060.10315297781237835No Hit
CTATTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1060.10315297781237835No Hit
AGTAATGGGGGGGGTGGTAAACAGAAGCCAGAAGGACATAGATGGGAAGAAG1060.10315297781237835No Hit
CATTGTGGGGATGATCTCTGTGAACATGGAAGGTGTGTTTGCTTTAAGTTAG1060.10315297781237835No Hit
TTGTATGGGGGGGTAAGAAGCCTGCTGAGGGATGCCCAAGAAGTTCCAGGGT1060.10315297781237835No Hit
TGCTAGGGGATCTAAAGTTGGTTTAACATACACATATCAATAAATGTGATTC1050.10217983651226158No Hit
ATTGCAGGGCTGGCGGTGGCGGAGGCGGCGGCCCCTCGCCTTGTGGCCCTGG1050.10217983651226158No Hit
AAATAAGGGAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAA1050.10217983651226158No Hit
TTAAGCGGGCAATTAATTGCTGTAAGAGACACTTCTAAATGATTTGGTCCCA1050.10217983651226158No Hit
GCCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAAC1050.10217983651226158No Hit
AGAACTGGGATATAAATGAAATACAATAATGTATAAATGAAATACAGTAATG1050.10217983651226158No Hit
CTAACAGGGGTTTGCTGTGTGATTGCAGTTTTAAATTATAACATTTCATAAA1050.10217983651226158No Hit
GCACCCGGGTACTTCCGTGAGTGCTAATTGTATTCTGATAGTATTTTTTTCT1050.10217983651226158No Hit
AATACCGGGTGGACAGAAAAGTGAAAATGGTTGGTGATGTGATGGTGCCATG1040.10120669521214481No Hit
AGGTTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1040.10120669521214481No Hit
GCAATGGGGACTCTTAGAAAACAACATGTTTTATATTTTTTAAATATTGTAC1040.10120669521214481No Hit
CATTGTGGTACTCTCTTGTTTCCTTTAGATGGAACAACAAATTTTGGGGACT1030.10023355391202804No Hit
TACTCGGGTATTCTCAATTCTTTTTTTTCCGCCCTTCCTTGGCAATGGCTGG1030.10023355391202804No Hit
CGCATAGGGCAATCTCAGCTGCAAGTAAACGCCTGTGGGTTAGGTTTCTTCT1030.10023355391202804No Hit
GAAAATGGGGAACAACTAGATAGAAGATCAAATATCCCACTTTCAGTAATGC1030.10023355391202804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)