FastQCFastQC Report
Sat 31 Dec 2022
SRR5848688.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848688.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102186
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC5900.5773785058618598No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT5830.5705282524024817RNA PCR Primer, Index 40 (95% over 22bp)
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA1970.19278570449963792No Hit
CCGATGGGGATTGGCATTTTAGACTTTAAAAATGCTGTATGATTTCAGATGA1630.15951304483980194No Hit
TTGACTGGGAAGTGCAGAAATCTTCAGTAAAATACCAGCAAACTGAATTCAA1510.14776975319515392No Hit
GAAACTGGGCGATCTAAAGGTGGGTTATAGAGAGAAAGAATAAATGAGTTTA1440.14091949973577594No Hit
GGTGCTGGGGGAAGAGGACAAGCCTGAAAACATAAAGAAGTAAATGGCTAAA1440.14091949973577594No Hit
TTTGCGGGGCAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGG1430.13994089209872193No Hit
GCGCAAGGGACTAGTTGGTAATGTGATTATGTGGTACCTTGGCTTTAGGTTT1420.13896228446166795No Hit
ATAGCAGGGGTCAGGGAGGAGGAGTAGAATTATGTGAGCTTACTTCAGAGCA1400.13700506918755995No Hit
ACAGGTGGGCTTTCCGAAATGACTTTGCACAACCACAGCCAATGAAAACATT1390.13602646155050593No Hit
ACGAACGGGGTTAATAATCAGAGAAATCATAACGAGATGGCTTCTAAAAATA1380.13504785391345195No Hit
GCATAAGGGACTTTGCAGATCTCTTATGAATTCTTTCACTTATTTCTCATTG1350.13211203100228994No Hit
GCATTGCGGGTGAGTGAAAGTTTCTAGAGTGTTTTCCTTGACTATTTTTGAC1320.12917620809112795No Hit
CAAACAGGGGAGAACACCAATGTAACATTTCTGAAACTAGTGAGTTGCAAAC1300.12721899281701995No Hit
TTAATCGGGGATGTGAATCTTCCAATTTTATCTATTTGATTCAAATGGTATG1300.12721899281701995No Hit
CCAAGGGGAATATGAAGATGTATCTTTCTACTTGTAAGGGCCAGTAGCAAAC1290.12624038517996594No Hit
CAAACGGGGATGTGTAGCACAGAACTGCACTAGGATTAATTTGTTTACAAGA1280.12526177754291196No Hit
TGCAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1280.12526177754291196No Hit
AAGAAAGGGCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1270.12428316990585794No Hit
CTTGTAGGGATTCATCTTTTGGAGGGTGGGGGGAAAACCCAAAAACAACAAC1260.12330456226880394No Hit
CATTGTGGTACTCTCTTGTTTCCTTTAGATGGAACAACAAATTTTGGGGACT1240.12134734699469595No Hit
CCATTAGGGGAGTTGCCTTAAAGAAAATTCAGAGCTGATATTCAGCCAGTAC1240.12134734699469595No Hit
TTGTTTGGGATATATCAATAATAACTTTGAATGTAAATGGCCTAAATCCCTC1230.12036873935764195No Hit
CATACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1230.12036873935764195No Hit
GCTATAGGGGTCACTAGTAAGAATGGACCCAATTCAACTTTTATCTTAAGTT1230.12036873935764195No Hit
TAATATGGGAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTC1230.12036873935764195No Hit
GTATCTGGGCCAGTTAACGCTCACTGGCCAGATGATCGCAGTGTCTCCAGAA1220.11939013172058795No Hit
TATTAAGGGGTGTAATAAAACAATGGCACTTGCTTATAATGGTCTTTCAGTA1220.11939013172058795No Hit
AAACTGGGGAATTGTAAGAATTGATTTTATGGAGCTTTTTGATATCTAATAA1210.11841152408353395No Hit
TATAAGGGGTTTTATATGATACAAGAGAAACAACAAAATTAAGTCTTACAGT1210.11841152408353395No Hit
CTATTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1200.11743291644647993No Hit
ACAGTGGGGGTATAAAATGTGTATTGGTTAGCATTTAGTACTAAGAAATTCT1200.11743291644647993No Hit
ATCCTTGGGACTGGAAAGGAAGCACATTCAAGTTCTTAGAATTCTGACTTAA1190.11645430880942594No Hit
AAGCCTGGGGTACTGCATTTATGCAATTAGCAAATAACTGCTAGTTTTCATT1190.11645430880942594No Hit
CTTATTGGGGTTTTGAAAATGCTACTGAATTTGTCTGAAAATCTTTTCATGA1190.11645430880942594No Hit
ATACGCGGGCGGCGGCCCCAGCGGAGACTAGGCCAGAACTGAGCATTTTCAA1190.11645430880942594No Hit
CTAACAGGGGTTTGCTGTGTGATTGCAGTTTTAAATTATAACATTTCATAAA1190.11645430880942594No Hit
GTGCAATGGGAACACAGATACCTACAGACTCCGTTTTCATTTTCTCGTGTTC1180.11547570117237194No Hit
TTTACAGGGAGGCTGAGAAAGGTGAGGAAGCTACAGAAGAAAATTCTAAAGC1170.11449709353531796No Hit
TCTAGCGGGGCGAGCACTTTTAATAAAATAATATGTACCATATTGGTATTGT1170.11449709353531796No Hit
CTGCGGGATGGTGCTAAGGTGGTTTTCTAGGTAACTGCAGGGATGGAGGTCA1170.11449709353531796No Hit
CTAGGTTGGGGCCACAATTTTCATGACTTTCTTCAGAAGTAGCACATTTTCG1160.11351848589826397No Hit
GGTAGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA1150.11253987826120995No Hit
GCTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCA1150.11253987826120995No Hit
TTTAGTGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG1140.11156127062415595No Hit
AGTGATGGGACTAGCTGAACATGTTTTGTGCCAATTGTGTGCTAGAGATAGT1140.11156127062415595No Hit
TAAAACGGGTAGAGTGAAATACCTGATTCAGCTGGCTCCTCACTGTCTTGAT1130.11058266298710195No Hit
GTCTAGGGGGGCACCGGCCGCGCGGCGGGAGGAGGTGCCGGCCGAGCACCGC1120.10960405535004795No Hit
ATAAATGGGACACCTGGATGGGGTGCATGTAGTCTTTGGACTGGTTATTTCT1120.10960405535004795No Hit
GTTTGGGGGGCTGAAAACAATCAGCAATGCCATACTACTGACACAAACGTTT1120.10960405535004795No Hit
TTCAACGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAG1120.10960405535004795No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC1110.10862544771299396No Hit
CAAGCTGGGAGATGGATGTAGTGAATGCTTTCCAGAGTGGAAGTTCCATTCA1110.10862544771299396No Hit
GCCTGTGGCTGACAATACTTCACCTTTCTTTTAATTCCCCATGATGTTTTCA1110.10862544771299396No Hit
GCATGTGGGCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTT1110.10862544771299396No Hit
GTACTGGGGGGATTAAGAATCTCACTCAAAACTGCACAATTACATGAAAACT1110.10862544771299396No Hit
GAGACTGGGATATACCCTTGTCAATAAAAACATAGAATGTTCATTGCCAGTC1110.10862544771299396No Hit
TGCTATGGGTCCCTTATATAAAATTGAAATGGTATCATGTTTGCATATAACA1110.10862544771299396No Hit
TGTTGAGGGCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAA1110.10862544771299396No Hit
CTCGGACCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1110.10862544771299396No Hit
AGACTAGGGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGA1100.10764684007593996No Hit
GTCCTCGGGGCAGCAGCCACTTATCAAACCCCTACTTTAATATTTCACTCAA1100.10764684007593996No Hit
TCATCGGGGGGAAATAATGGCTGAAAACTTATCAAATTTAGCAAAACAACAT1100.10764684007593996No Hit
CAGCGCGGGTCTCATGTGCAAGGCTGTGATGGCTTCATGATATGATGGATTA1100.10764684007593996No Hit
CTGTACGGGATTGTTGGGACAGGTGCCATTTAAATTGCCTCCATGCTCCCCA1100.10764684007593996No Hit
TGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1090.10666823243888596No Hit
TCGTTGGGGACTAGGAGATCTTCCACAAGTCACTTAACTTACTTGAACCTCA1090.10666823243888596No Hit
GTTTTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1090.10666823243888596No Hit
TAACGTGGGAGGATTTTGATGGAATGAGGAATCAACTTTATTGCGCTGAGCC1090.10666823243888596No Hit
TACCGCGGGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTT1080.10568962480183196No Hit
CTAGTGGGGACACCGAATCTTGATGAAAAAAGAGAAGAGCATTTGCCTTGTT1080.10568962480183196No Hit
TTGGACGGGGGAACCCAAGACTTTTGGTAGTTGGATTTGCTCTTTAAAGTTT1080.10568962480183196No Hit
CTCTGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1080.10568962480183196No Hit
TCACATGGGTCAGGGCCCAGTGCACTCACTTCACAGTGACAGCCCTCCCCTA1080.10568962480183196No Hit
CGCATAGGGCAATCTCAGCTGCAAGTAAACGCCTGTGGGTTAGGTTTCTTCT1080.10568962480183196No Hit
ATGCTTGGGAGAGAGTGCTTATGTTTTGTTTACTCATGTTTCAACTTGCTGT1080.10568962480183196No Hit
TATTACGGGTATTGATGTCTTCGCTCAAGACATGTGTGGACGAGATGCAGAA1070.10471101716477794No Hit
TTGCCGGGGGTTCTAGATCTGGGAAAGAATTACTTGAAATACATTATTTAAA1070.10471101716477794No Hit
TGCATGGGTAAGCCACATTGATTTATCCTCATTGAGGATTATGGTCTGTGAC1060.10373240952772395No Hit
TTAAGCGGGCAATTAATTGCTGTAAGAGACACTTCTAAATGATTTGGTCCCA1060.10373240952772395No Hit
GACGAAGGGCAGACTAAACAGAAGCAGACCATCCACCTGCAGGCAGACCAGC1060.10373240952772395No Hit
ATGCCTGGGGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGA1060.10373240952772395No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTA1060.10373240952772395No Hit
GAAGGCGGGATAGAAAGTAGCACCATCAACTGTGGAAAATGACCACATCCTT1050.10275380189066995No Hit
TTCAACGGGATGGCTGTAGATATGCAGATATGTCTTACACAGACAGAAAGTT1050.10275380189066995No Hit
CTTTCAGGGCCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGACC1050.10275380189066995No Hit
AACAAGGGGAAGCTCCAATCATCGGCCAGACAGTTGCTTTATGTAGGTTTTT1050.10275380189066995No Hit
GACTTCGGGCTCCGCTCGATCTGGCCAAGCTGAACCAGGTGGCAAGACAACA1050.10275380189066995No Hit
AAATAAGGGAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAA1040.10177519425361595No Hit
AATTACGGGAAGAGAAGAGAGCAAAAGAGTGAAATTGTAGTTTTCTTCTATG1040.10177519425361595No Hit
AGCACTGGGGAATTACTAATATGATACAGTTTCTGATTCCAAAGTATAAGAA1040.10177519425361595No Hit
GCGACAGGGCACTATCATGAGAACAGCACAAGAAAGACCCACCCCCATGATT1030.10079658661656195No Hit
ATCTAAGGGAAGTATTTGGGTGAGAACTGAGAGGTGGGAGATGATTGGAGGC1030.10079658661656195No Hit
TCACATGGGAGACAAGCTGTGTGAATGCATAGATGTAATCTTGTCTCAAATA1030.10079658661656195No Hit
ACCTTAGGGGATCTTTAGTCGGGCTCCCTAAAGCCCATGTCGAAGCCCCCAT1030.10079658661656195No Hit
GATGCTGGGAAAAGGAAAAATAGTAGTAACTGCTCTGCTTTTTTACCATACT1030.10079658661656195No Hit
TGCTACGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1030.10079658661656195No Hit
GCCATTGGGGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA1030.10079658661656195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)