FastQCFastQC Report
Thu 19 Jan 2023
SRR5848685.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848685.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403476
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT12020.2979111520883522RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC10140.251316063408976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG206.2705384E-446.053491
ATCGCGA206.2705384E-446.053491
ACGACAG206.2705384E-446.053491
GTTTGCG253.3898286E-546.0534861
ACTTGCG453.0559022E-1046.0534861
GTTTACG253.3898286E-546.0534861
TTTCGCG301.8435603E-646.0534861
GCGCACG253.3898286E-546.0534861
AACGATG301.8435603E-646.0534861
CGCAACG301.8435603E-646.0534861
TCGCCAC206.297434E-446.0135245
GGCCGTC206.297434E-446.0135244
CGTTAAG206.297434E-446.0135242
GAGCGTA206.297434E-446.0135240
CAGGCCG206.297434E-446.0135242
AGATTCG206.297434E-446.0135246
CACAACG206.297434E-446.0135244
CGATCAC206.297434E-446.0135240
CCCGCCG206.297434E-446.0135245
TGCGTCG206.297434E-446.0135241
Produced by FastQC (version 0.11.5)