FastQCFastQC Report
Sat 31 Dec 2022
SRR5848678.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848678.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81213
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT6240.7683498947212885RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC6140.756036595126396No Hit
ATATGTGGGATCCTTCAGTAATGAATCACAAATCAAGACATTCTCAGATGAA1610.19824412347776835No Hit
CACACAGGGACAACTTGCACCACTAAGAAAAAAATGTAGAACCATTTGGAAA1480.18223683400440815No Hit
CATAATGGGGGAGCCAGGCCGGAAGGAGGCTGCCGGAGGGCGGGAGGCAGGA1410.17361752428798344No Hit
CCAAATGGGGAGGATCTCAGATCTAGATATGATTTCTGGCACTGGATCATGT1400.1723861943284942No Hit
GCGAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTC1380.16992353440951571No Hit
CAATATGGGACAAACTCTACTTCATTTTTCCTACCTCCTGTGCAGTGAAGAA1290.15884156477411251No Hit
CAATTAGGGAAAATGGTGAAACCCTGTCTCTACAAAAAATGCAAAAGTTGGT1280.15761023481462327No Hit
GTAATTGGGATGCTCACGCCTGTAGTCCCAGCATTTTGGGAGTCCAAGGCAG1280.15761023481462327No Hit
AACTAAGGGTACCCGGGTGACATACCCAGCCAAAGCCAAGGGCACATTCATC1240.15268491497666628No Hit
AGCGCTGGGCAATGAAACAATGAAACCAGAGCTTCTAGGTGTGTGGCCTGGA1220.1502222550576878No Hit
GCTATTGGGACAAAACTATTGTGCAAAAATCACTAGCAATCCTATACCCCAA1210.14899092509819856No Hit
TGCGATGGGATTCAGTGAAATCACAGCCAAGGTTAGCCAATGTGGGAGACAA1210.14899092509819856No Hit
ATTAGCGGGGGTCATGATTCTGGCCTGTAACACCAAGCAGTATAACGATGAG1200.14775959513870932No Hit
AAAATAGGGACAAACACATTATGCAGCTGTACAAAAATATTTTTTATTTATA1200.14775959513870932No Hit
AGAAACGGGATTGCTGGGCGTCTGGACATCTGTAGCTGTCGTTTGGTTTGAT1200.14775959513870932No Hit
GCAAGGGTTTTTATTTTGTTTTAATGAAGGGCAAGAAGAAATTGTACTTTTC1180.14529693521973083No Hit
TGAACCGGGCTCCATACTGGAGCCTGAAAGATCTTTGGAAATGTAGATCTGA1160.14283427530075235No Hit
TTTATAGGGCAGCTTCCACCATCAAGCAATCCACATACTTAAAAAGCCATCA1140.14037161538177384No Hit
GTGGCTGGGAGCAAAGGTGATGAAGGAGTTTGTGGGTGGGAACACACCCAAT1130.1391402854222846No Hit
GACTTTGGGAGGATCCCAGCAGTGGCTGCACCTCCAAGACCCTGGCCGTGCC1130.1391402854222846No Hit
CTCCTAGGGCTCTCAAAATGTGCCGTCTTTGGTTCCTCATGGCTGTTATCTG1120.13790895546279536No Hit
CGTTTTGGGCACCTCTTTCTCTTAGAAGATGGGATGAATTCAAAGTGCCTCC1110.13667762550330612No Hit
TAACTGGGGGATCTAAACTTGGGCTGTGCATGTATCCCAGAACTTAAACTAT1110.13667762550330612No Hit
TCTTAAGGGAACCTCTCCTCTTTTTCCTCTCTTGGTGGAAATGTTTTGTTGT1110.13667762550330612No Hit
TATTAGGGCACCCATTAAGACTTACATTAACATTCCTTTTATATAAAGAGTT1110.13667762550330612No Hit
AAAAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1110.13667762550330612No Hit
TTGCTCGGGATGGTGAAACACTGTCTCTACTAAAAATACAAAAATTAGCTGG1100.13544629554381687No Hit
GCTACTGGGATCAGCTTCATACAATTACAAGTCTTGCATACATAAAACAGCT1100.13544629554381687No Hit
TCGCCAGGGATTCTTGTTAACTAAGCATTGTGCTTCCCAGGTGGTCTGAAGT1080.1329836356248384No Hit
ATGATGGGGTCTATTTAAGTTTTCAAAGACCAGAATTTCTTTTATTCGCTTC1080.1329836356248384No Hit
AGTTCAGGGTATGATGAAATTGTAAATTAAGAAAAGACCAAGTGATTAAATT1070.13175230566534915No Hit
TAAACGGGGACCCATGAGCCCCCTAGATATCATAAAGTTAGATTTAATAAAT1060.1305209757058599No Hit
GTAAATGGGACATGGAAATAGCTTGCTATTTTTATATACATTTCCAAAATAT1060.1305209757058599No Hit
GGTATGGGGAATTCTGCATGTTATGTTTTATTTCTGCATCTTTTCAGAGAGG1060.1305209757058599No Hit
GACCAGGGGAATGCTTCTAGGCGGACTATGACTTAGTTGCGTTACACCCTTT1060.1305209757058599No Hit
TAAGGTGGGCTACAGCGGGGAGGTTACAAGAGCTATCTATTTTAGTTAAAAT1050.12928964574637067No Hit
GCTTTAGGGCGACGAGGGAACTCTATTTCACTTTTGCTTCATTTCATCCTTT1050.12928964574637067No Hit
CTATGAGGGCGACGCTCTTCCGATCTTTGAATGGGCTTTCCACAGATGCAAA1050.12928964574637067No Hit
GTGGGCGGGATTCACTGCATATTTGTCTGCTAATTTTTCTTTAATGAACAAA1050.12928964574637067No Hit
ACCGTCGGGTCTTTTAGTCTTGTGTGAATTTTTTTAGTAAAGTTGATGTTTT1050.12928964574637067No Hit
TAAGCACGGGCATCAGAGTGGAAACCCGTTCAGTGTTAGCCACTCATTCATC1040.1280583157868814No Hit
ATCACAGGGAAGATGCAAATCATGGAGTTCCTGGCCAGGGTCTTTAAGAAAG1040.1280583157868814No Hit
GTGGCGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1030.12682698582739216No Hit
AGGCAGGGATTCCCGCATGAGACTTAAGCTGGTACCGAATTATAATTTCAAA1020.12559565586790292No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC1020.12559565586790292No Hit
CGTCAGGGGATGGAAAGGCCGAGCTTTGTTTCCTGTGTTTGTGTGGACTCCA1020.12559565586790292No Hit
GACACGGGGAGTTCACTGATACTAGGAAACTAAATAATTTAAATCCCTAAAT1020.12559565586790292No Hit
TGAATAGGGGGTCTCTTTGATTTAAAATGAATAAAAATAGATTATTACGTCT1010.12436432590841368No Hit
CATAAAGGGATACATAAACAATAAACCATTGCGTAGGAACCATGAAGAAAAA1010.12436432590841368No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCCTGTCTCTTATAC1000.12313299594892443No Hit
TAGAACGGGGAGATATGGTCTGATTTATTTTTTTAATTCACCTTACATTTTA1000.12313299594892443No Hit
AGCCATGGGGATCTACGCCGGGTATTTTTACTTTCATGCAATTTTTTTACAC1000.12313299594892443No Hit
CGCTCAGGGAAGTAGGGTGGCCTCACCAAGGGTGTGGTTCTCAGTCTGTACC1000.12313299594892443No Hit
TCGTTCGGGATGTCCATCAATAAGTATTTATTTATTGAGCTACTTGTGTTTG1000.12313299594892443No Hit
GGCAGGAGAAGGAAATAAAGGGTATTCAATTAGGAAAAGAGGAAGTCAAATT1000.12313299594892443No Hit
TTAAAAAGGGATATGCACCACTGCCATCACAGTCCTCATCGTGATGCAAGCT990.12190166598943519No Hit
TTCTTGGGAAATGCTAAATTACTTAAAGGTAAAGTGCCACGATGACTAAAAC990.12190166598943519No Hit
TCGATGGGATGGAACCAGAATCTAAATCCAAATAGGCTGTTGCCAGTACAGA990.12190166598943519No Hit
CTTTTAGGGAAGGCTGGCAGTCCTTGATACCTATTTCTTCACCTCCACTTAC990.12190166598943519No Hit
TTTAACGGGTGGGATGAGGTAATAACTCATTGTGATTTTGATTTACATTTCT990.12190166598943519No Hit
AGAGCAGGGATATAAGACATTGTCATGATTACTTAGAGAGTATATGACTGAA980.12067033602994595No Hit
CTACAAGGGTGGCAGAAAGCAATAGAAAATAAAATTAAAATTTTATTCACAA980.12067033602994595No Hit
TTGTTCGGGGATGGCTGAATCCTTATGAATGGGATTAGTGCCCTTACAATGA980.12067033602994595No Hit
TCCAGGGGGAATGGGTTATGATCAGCATTCTTTTAGTGAAATGAAACAAGAT970.11943900607045671No Hit
GGCTTAGGGGCTTTTTCTTCTTTCTAGAATGGTGTGTTTATTTATTTATTTT970.11943900607045671No Hit
ACACAGGTACCCCATTTGATTTCGATGTACAGTCTCAATTTTGTATTTAATG970.11943900607045671No Hit
TCGTTGGGGTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTT970.11943900607045671No Hit
TGACTAGGGACCTTGGTAGAAATTATTAGTATCACCTGCCATATTTTCACAT970.11943900607045671No Hit
CTACAGGGGAGTTGTTTTGGGCTTCTATTTTTGAACTGTTAAAAAATTTTGA960.11820767611096747No Hit
TAAGACGGGAACTTTTCATTCTAAAGCACACTTGTTATTTTATAAACCGAGT960.11820767611096747No Hit
TCTAGGGGGCTTAAAGATGATATGAAAAAAGGAAACTAAGTGGTCTAAGCAA960.11820767611096747No Hit
CTGTCAAAGGAGAAACGACTGGGAAAGTTTGGGAAAGTCAGCAAAAGAAAGA960.11820767611096747No Hit
TGAATCGGGAGACAAGTGTGAATTACAAGAAGAAAACCAGTATTTGATTTAA950.11697634615147823No Hit
GCCCAGGGATTCTGTCTGAAGTCTCTGAGGGAAAGTAAAAACAGCTCTAGAA950.11697634615147823No Hit
TCTACAGGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTG950.11697634615147823No Hit
TGATGTGGGATCAGATTTGAAGTCCAAACAACCAGTTAAGTATCCTGTTATT950.11697634615147823No Hit
ACAAATGGGATGTAGGCTGGGAGGCTAGGCCAGTCTCGCCTTTTCACATTCT940.11574501619198896No Hit
GCTTCTGGGGTTTTTACAAAAAGTAATTTTTTAAATTGCTTTTGTCATGGAT940.11574501619198896No Hit
ATTAAAGGGAATCATATAAAAGTAAGTTAAGTAATTATCACTACAAAAAATA940.11574501619198896No Hit
TTTCCGGGGGCCAAATTTTATATTTTAGCAGGTTTCATTTTATTGATATATT940.11574501619198896No Hit
CGAAATGGGAGTGTATACTAAGCCTTGTGTGGGTGTTTTACATGAAGCAGAT930.11451368623249972No Hit
TACTGTGGGCAAAGTACTTGGTCTCTCAATTTCTTGATCTGGTTTTGCTTCC930.11451368623249972No Hit
ATCGGTGGGACTGCTTTTCCAGACACAAGGCTGCTAAACCAATTAAGAAAAT930.11451368623249972No Hit
AACTGTGGGATGGTCTTGTTTGTTGACATGACAAATTAACATTCTTATAGTT920.11328235627301048No Hit
GATGTTGGGCAACTCAAAATGAACTACACATTTAAACATAATTACCTGAGAC920.11328235627301048No Hit
AGAACAGGGATTCGTGTTATATGATGAGGCATTCTTACACAGATTATTTTCT920.11328235627301048No Hit
TGTAGGGGGATAGGCGTGAGCCACTGCGCCCGGATAGTAGCTCCATTTTATA910.11205102631352123No Hit
GTAGCAGGGCTGCTCTGTAAAGTGCTTTCCTGTTGAATTTTCCATTCAAATT910.11205102631352123No Hit
TATCAAGGGCAATTTGGATTTTCTGCTTGTTTAAGTTTGCTTAAGTTCCTTG910.11205102631352123No Hit
ATCTGGGGGATACTAAAACTGGAACGAGATTGGGAACAGGCAGAGAGTTAGG910.11205102631352123No Hit
TACACTGGGAGGATTTCCTACCAAACATTTTCCTCTGAGGTTTGTTAACTCA910.11205102631352123No Hit
TTAACAGGGGACCAGATTCGTGACCACTCTGAATCATATCTTGACAGTTTCT910.11205102631352123No Hit
GCTTTGGGGACCGTATTTCATAGAAGGGAAGGAAAAATGTAAGGTTTAACAG910.11205102631352123No Hit
TGAAAAGGGGTCTGAATTGTTAAACCACAGTGCTTGCTAAGACAGAGTCAGC900.110819696354032No Hit
CTAACTACGGGGGGTTATAATTTTGCATTCTTTTTGACTTTGTATGGATGAC900.110819696354032No Hit
CAGCCAGGGAGTTAGCCTAAGTTATCAGCTGTCTGAAGTTATTCATAACCTA900.110819696354032No Hit
TTCCTGGGGTCTTCTGCGTACGGTTAGCCAGTTCTCCCAGCACCATTACCAT900.110819696354032No Hit
AGCGAAGGGACCTGGCATTCCTCTCTGTTCATGGAGCTTCTTTGAGGCTTGA900.110819696354032No Hit
GGCGATGGACCCATTTGAGTTTTTGTTCTTACAAGAGGTCCTGCAAGCCTTC900.110819696354032No Hit
TATGGCGGGGCACAGATTTTTAGACAAATTTCTTGTATCAGGAAGAAATACA900.110819696354032No Hit
GCTTGTGGGCATTATGAGCAAAAGAAGTCAGACGTAAAATAGCACATACTGT900.110819696354032No Hit
GTTGCAGGGCAAGTTTAGCAGCTGCTGAGACTTTTCTCTTGAACTCCATGTT890.10958836639454275No Hit
ACAAAAGGGAAGCGTACCAAACGGGCAAGGACATCTCTACAAATTACTATGC890.10958836639454275No Hit
TGCTTCGGGGTGATGCACTGATCAATTTTTGTCACAGCATTTTCATACCTTC890.10958836639454275No Hit
ATAGGTGGGACAGGAGGGGAAGACAGACAAATTATATTCTGGAAATTGCAGT890.10958836639454275No Hit
ATATAAGGGAGTCAAGTGACGGTGCTGAAATACTTTTCTCATTACATGGAGG890.10958836639454275No Hit
GCCACAGGGCAAATCATGTCTGTAAAACTTGACGTATTTTGTGTATGCATGT890.10958836639454275No Hit
TAGCCTGGGACTTCTGTAAGATTGTTTTAACTAGACTCCCCCCACCTCCCCT890.10958836639454275No Hit
CTATATGGGACAGACCAATTTGCAAATGATCTTCAATGTTAAGCACTTGCTC880.10835703643505351No Hit
TGTGCTGGGAACGTGGGAGTGCATAATTCAGGCTACCAAGACCATTTAACAG880.10835703643505351No Hit
CATGCTGGGATTCCAAAAATGAGTTTCATTCAAGTTATTTTGTTTTCAACAT880.10835703643505351No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCCTGTCTCT880.10835703643505351No Hit
CGTGGCGGGATGTTTTTATTAGTTGCCTTTGTTATATACCGTTGGTTGTATG880.10835703643505351No Hit
TTTGTTGGGGAATTTTCAAAAGAAACAACTAATCAGTTGCTTGCATTTTCTC880.10835703643505351No Hit
AGATCTGGGCGATCTATGTGAGGGCAAATAAAGATACATGGATAATTGTAAA870.10712570647556427No Hit
GTGCAGGGGTACTCTGTCCCTCTTTTTATATGGTATTCTGTGAGCTAACTAG870.10712570647556427No Hit
AGTGGCGGGGCTGGGGGCGCTGTTCTTTAGCTAGTGCCAGTATTAAAACATT870.10712570647556427No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGA870.10712570647556427No Hit
CGCTTAGGGCAGGGTATCTTTATATCCTTTCCATGTGAAAGAGATATAAAGA870.10712570647556427No Hit
CACAACGGGATAGGTTGCAGTGAGCCGAGATTTCTCCACTGCACTCCAGCCT870.10712570647556427No Hit
CGCTATGGGATGAAGTGAATGAAATGATGAAATGAAGAAATGAAATGATGAA870.10712570647556427No Hit
CGTATGGGCAACACATTGGAATTTTGTGACTAAATGCCCACAATCCCTATGT860.10589437651607501No Hit
AATGGAGGGATGAATCATCAATGTAGACATTATTGAAGCCAGAGAAGTGATT860.10589437651607501No Hit
AAATTAGGGAGAAGAGTGAAAGTGGAAATTTGTTTCTAGCTTGCTTCATTGC860.10589437651607501No Hit
AACGTAGGGGGGGCCATGGGGCTGTTATTCTGGCCAGGGACACTGTACTTTT860.10589437651607501No Hit
CTGGAAGGGCGTTTTCATTTTCTTAGATGACTTCTTGTCTGAGACAGAAAAA860.10589437651607501No Hit
TAACTAGGGATAGCTTCACTGATGAATTCTACTAAATATTATAAAGAAGAAT860.10589437651607501No Hit
CATTTTGGGCTTGTGGTGGCCTCAGTCTATAAAGCCCAGGGTTTATAGAAAG850.10466304655658577No Hit
TAGTGTGGGACCGACTCCGACCTCTGTACCCCGTGCGGGCCCCCGCCACGCT850.10466304655658577No Hit
TGCAAGGGGATTGATAAAAATGCCTAATAATATATGTGTAGAAAGAATTTAC850.10466304655658577No Hit
ACGTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA850.10466304655658577No Hit
GGTCAGGGGACAGAGCCTTTTTTTTAACCCGCTGTGTCCAAGTTGGGCCCTG850.10466304655658577No Hit
CAATCAGGGATTGTTTCATGGGAACTCCACCACGGCCATATGAAACACTAGT850.10466304655658577No Hit
TTAGGCAGGGAACTGTAAATGCTTGACTTGTAAATATTTGACAAGGTTGACT850.10466304655658577No Hit
TCGTACGGGACTCCTTCACTGCTTCTTAGAAGGTAGAATTAAGTTTCTGGAA840.10343171659709652No Hit
TCTCATGGGTAATATGAATTCCTGTCTAACAGGCTCTGTATATCTTGTAGGT840.10343171659709652No Hit
AATCCGGGGTCAGTTTCCCCTTTTCTAAGTGCTTCCTAGATATATTTCTTGT840.10343171659709652No Hit
GATCGCGGGACTCTTCAGTGTTCCTGTTATTTATGAACGGCATCAGGCACAG840.10343171659709652No Hit
GATACGGGATTTGTTCTTTCCAGAGCTAACTGATAAGAGTGGAGGAGGAATG840.10343171659709652No Hit
TGCAGATGGGTATAAAAGCAGATGGGCCTCCCATTCCAGACTTATGCATTAA840.10343171659709652No Hit
GATATCGGGGGATGTGACTATTAATGAGATTATTAAAGTCCCAATATCTCTT840.10343171659709652No Hit
TAGTTTGGGAGAGGCTCTAAAAATAACAAATGTCCACCACCTCACTCACTGG830.10220038663760728No Hit
AAATGTGGGGTGGCTGAGTTGGCAACCAAGCTGCAAGACAATGTCCTTCCCA830.10220038663760728No Hit
AAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG830.10220038663760728No Hit
AGAACCGGGTGCAGGATCACAATATGTATAATGATTCCCAAGGTGGAGATCT830.10220038663760728No Hit
CGTGGGTGCCTACCTGAAGGCAATAGTACTCTTCAGTCCAGATCATCCAAGC830.10220038663760728No Hit
GCTAAGGGGGATCTTTTTGTGGGATGTTCGCCGACCGTTGACTATTCTCTAC830.10220038663760728No Hit
ATTTATGGGATTCCCAGATTCTTTCCTGAATAGATTTGAATTTAATGTCATT830.10220038663760728No Hit
TTTCTTGGGAGGAACTATTTTAAGAAAGAAAAAAATCCCTCATTCAGACTTC830.10220038663760728No Hit
TGATTCGGGCATTAGAAACCTCTCACAATGCTCAGGACCCTGAGATAGGTAA830.10220038663760728No Hit
AAGGTTGGGAGCTCTTACTATTTCTTTTATTTTTCTGCTCATTTCTGAATGA820.10096905667811804No Hit
GCTAACGGGAATGCAAATTCTTCTTCAAGACTGAAGACATTGATTAGATATA820.10096905667811804No Hit
TGAATAGGGATGTAACAAACCTGCACATGTACCCCTGAACTTAAAAGTATAA820.10096905667811804No Hit
GTAAGTGGGGAGGCAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG820.10096905667811804No Hit
GGGCTTGGGCAAAGCACAAGGGAAAAGTTTTGTGACCTTAAGTTAGGGAAGA820.10096905667811804No Hit
GACTGGGATGACTTCCAAGGCTACAGCTATGATTAGATATGTGTTTCATGTC820.10096905667811804No Hit
ATTCATGGGCTAGGCAAATAAAATTTGATTTTGTGAATAAAGCTTGTTTCTT820.10096905667811804No Hit
CGCACAGGGTCCAAAGAGTAAATGGCAGGCAGCCGTGGCTAAAAGTAGTCTT820.10096905667811804No Hit
CTTAGTGGGTACTGGAGTAACCGCTTCCTAAACCATTTTGCAGAAATGTAAG820.10096905667811804No Hit
TTTCATGGGATTTGCTCTTGTCTTCAGAAAAGAAAGGAAGAAGTATCGTTCC820.10096905667811804No Hit
CAGCTTGGGATTCTTTGTAACTGAAGGAGAAATAAAGTCTTCCCCAGAAATT820.10096905667811804No Hit
TAGCAAGGGCAATCCATCAAGCATTTTATTAGAAAGACTTTATAATATTATT820.10096905667811804No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)