FastQCFastQC Report
Sat 31 Dec 2022
SRR5848675.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848675.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148997
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT10770.7228333456378316RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC9290.6235024866272475No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC1640.11006933025497158No Hit
GTCAATGGGACAGATGAAGTGTAAGATTAAATGAATAACTTATCCAAGGGGA1560.10470009463277785No Hit
GTTAACGGAGTATGAATTGCAATGTTTTCTGTTTCTTGTCCTCATCCCCCAT1550.10402894018000362No Hit
TTGTCAGGGAGAATTCACAGGAGCAGTTTCATCACTCCTTATTTCTTTGTAG1520.10201547682168097No Hit
ATAGTTGGGATTGAGCAGTGTTCTGAAAGTTGGATGGTTCAGTTTTCACTTT1520.10201547682168097No Hit
GACATAGGGGATCTTTCGGTGGGGATACTGATATAAAAATAAACACATAGAA1490.10000201346335832No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)