FastQCFastQC Report
Sat 31 Dec 2022
SRR5848674.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848674.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148048
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT10560.713282178752837RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC8670.5856208797146871No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAACTGTCTCTTATACAC1670.11280125364746568No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCCTGTCTCTTATAC1670.11280125364746568No Hit
TCAGCCGGGGACTTTGAGGAGCTGAATACTTCAGTGGAGGAAGTAACTGAAG1610.10874851399546093No Hit
CTCAGGCGGGAGTACTTTCCAGCAGTCAGTCCCTGGTTCCTAGATGTGTTCT1570.1060466875607911No Hit
TGACACGGGATCATTTCTGCTCAGTGTATGAATTTGGATTAATATTTAATGA1570.1060466875607911No Hit
GTCAATGGGACAGATGAAGTGTAAGATTAAATGAATAACTTATCCAAGGGGA1560.10537123095212364No Hit
TGAACAGGGAGCTGAATTGTGGTGCAACTTTGGGGCTATTTTAGGATTTTAA1490.10064303469145143No Hit
TAATGAGGGATTCTTTAAAGGAAAAAAAATCAGGGAACCGAAAAGTAAGATT1490.10064303469145143No Hit
GCGGCAGGGCTGTCACCTAGCAATCTGTTATTTTCTTTTATCCTTTCCCCAA1490.10064303469145143No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)