FastQCFastQC Report
Sat 31 Dec 2022
SRR5848673.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848673.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147566
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGCCGTCT10010.6783405391485844RNA PCR Primer, Index 40 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCCAGAATCTCGTATGC8480.5746581190789206No Hit
CTGATGAGGACAGATGACATGATCCTTTATGTAGAAAACCCCAAAGACTCCA1750.1185910033476546No Hit
TTGTCAGGGAGAATTCACAGGAGCAGTTTCATCACTCCTTATTTCTTTGTAG1730.11723567759510999No Hit
ATAGTTGGGATTGAGCAGTGTTCTGAAAGTTGGATGGTTCAGTTTTCACTTT1510.10232709431711913No Hit
TCAGCCGGGGACTTTGAGGAGCTGAATACTTCAGTGGAGGAAGTAACTGAAG1480.10029410568830217No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)