FastQCFastQC Report
Sat 31 Dec 2022
SRR5848636.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848636.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239445
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC9520.3975860844870429No Hit
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT8190.3420409697425296TruSeq Adapter, Index 10 (95% over 21bp)
CTACAAGGGACTGGGAAAGGGGAACGCACAGCACTGGGAAATGGGAGCATAG2620.10941969972227443No Hit
CTAACTGGGGTATTGGGAAATGGGAGCACAGTACTGGGAAAGGGGAACGCAC2550.10649627263045794No Hit
AGTGGGGGGAATGTAGTTGGATTTAAGTCGTTGTTTTACGTCTCAAGAGGCA2450.10231994821357723No Hit

[OK]Adapter Content

Adapter graph