FastQCFastQC Report
Sat 31 Dec 2022
SRR5848621.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848621.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155346
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT9610.6186190825640827TruSeq Adapter, Index 10 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC9050.582570520000515No Hit
ATCGCTGGGCACAAAAAGTAGAAAGTAAGAAATTGTATCATACCACCAGAGA2250.1448379745857634No Hit
ACTGAGGGGTGAAGGTCGTCACCTGGTTGGCCACGCCCAGCTCCACCGTGGC2150.1384007312708406No Hit
ATAGCAGGGATTACATAAAAACTAGCCATGCCGGATCACCTGAGGTCAGGAG1960.12616996897248722No Hit
TTTCGCGGGGCCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCC1950.12552624464099493No Hit
AAACACGGGGTGTTGTGAATAGATTTTATTATAAATCAGAGAACTGTTTTCT1940.12488252030950266No Hit
CGGCACGGGATGTGTGCACAGTTTATACAAAAAGGCCCTCATGGAAGGCTGG1900.12230762298353352No Hit
CAGCACGGGATTTTAAAACAGTGTCTGGTACATAGTAAGTACTAAATAGGTA1890.12166389865204126No Hit
GATACTGGGAGGAAGAGGAAGAAGAAGAGGAAGAAGAAGAGGAAGAAGAAGA1890.12166389865204126No Hit
TGCAGAGGGAGCATCGGCAAATGTGGAAAATGAGACGACTTATAAAGGTGAG1860.11973272565756439No Hit
TTGTAAGGGATGGCACTTAACTATTTGAAAAATAAATCAGTTAAATTTTAAA1850.11908900132607213No Hit
CGCAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1850.11908900132607213No Hit
AATTTCGGGTCAAAAAGATAACCTACCATGATCAAGTGGATTTCATACCAGG1850.11908900132607213No Hit
ATTAAAGGGGATGATGCTTTTTCTTGATGCTAAGATCACTGCAGAAGAATAA1840.11844527699457984No Hit
ACACAGGGGCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA1830.11780155266308756No Hit
ATGCGAGGGGGCGTATGTGAGGTTAACCTCGTGTAATGTGAGATGATTGCCC1830.11780155266308756No Hit
GCATAAGGGACCCTCCAGGGACTTAGTTTAATTTTACGAAGCCCAGCTATAA1830.11780155266308756No Hit
TAGTTGGGCAACTCGAACTGCTTTCGTCCATGTTTCTCACATTGCTGTATTT1820.11715782833159527No Hit
GCAACAGGGTCTAGCCAAGGGGGATCTCAACGAAGGCAAACACCTTTACACG1810.11651410400010301No Hit
GACAAGGGCGTTTCAGGGCTATTAGGCTAGTGAAGAAGGCTGGAGAGGGCAG1780.11458293100562617No Hit
CTTCACCGGGGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACCGGAGCGG1760.11329548234264158No Hit
CAATTCGGGTGATCCACGTTTTTGTTTTTTTAATGTTAAATGTGTAACTCAG1760.11329548234264158No Hit
GCGCTGGGGCTGGATTCTAATTGTATATCCAAATATTTATTCAATAACTATT1750.1126517580111493No Hit
ACTCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1750.1126517580111493No Hit
TTATTGGGGATTTTTGGAGGGATGATGTGGAGCTTAAATTTGGATGTGTGAA1730.11136430934816474No Hit
TTCAGGGGCAAGCAGAATGATGTAATTTAAAATAATACCATCAATTTTCATT1730.11136430934816474No Hit
TGTTATGGGAACTATGTGAAAGAAATTGAAAATTTGAAAAATGAGTTGATGG1730.11136430934816474No Hit
CGCACAGGGGACACCAGCAAGTCAGGCCTCTGGAACTTCTGATTGACCAGCT1720.11072058501667245No Hit
TAACACGGGAGAATGATTTCTTATGGTTTATTTTGATGAAAGTTCTTTTAAC1690.10878941202219561No Hit
GAACGAGGGAGTTGCTCTGGGATTGAGCCTAAGAAATTTTTTAAAGTTCTAC1690.10878941202219561No Hit
CTTGTTGGGGAAACTCGTAACAATTACCTCTCTACGTGATGCTGTAAGGAAT1690.10878941202219561No Hit
CCAAAAGGGATATAACATGTTTTCACTTGAGGGCTTAACAACTTGAGTGGTT1670.10750196335921106No Hit
ATAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1660.10685823902771878No Hit
TTCTATGGGTCAATGAATTTTTATAAGTAAACACACTTGATTCAAGAACATT1650.10621451469622649No Hit
ACGGCCGGGGCTGTGCTCTGGGACAGGGAGGCTGAACAGCTGCTGCCTGTCA1640.1055707903647342No Hit
GTGAACGGGGTTCTTTTTACCAATAATAATTTATCAAAATAAGAGGGAAAGT1640.1055707903647342No Hit
GTTCATGGGATGATCTTATAGGTAGAAAATTCTGAAGATTGCACACACAAAA1640.1055707903647342No Hit
TTTCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1640.1055707903647342No Hit
GCTTAGGGAGAGTCAAGATAAAAGAGTGGGAAAATATATATCATGCAAATGG1630.10492706603324194No Hit
AGTGCAGGGCATGAAATATAGTTGAGTAGTTTTTTGTTAAAACAGATAAATT1620.10428334170174965No Hit
CTGCTAGGGGTATGCACAGGAGGCCCTTGGGAGCCCTCAGATAACTTTCTCA1620.10428334170174965No Hit
TGTTGTGGGACATTCTAGATAGCAAAGGGGCACATTATCTCAGAATCCAAGA1620.10428334170174965No Hit
CTACGCGGGCTTCACTCACTGCAAACTCTGCCTCCCCAGTTCAAGTGATTCT1610.10363961737025736No Hit
CCACCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1610.10363961737025736No Hit
TTACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1610.10363961737025736No Hit
GAGTACGGGGGCGCGCAGCCAGGTGCGCTCCTTGGGGCTGCTGGCTGCTGCG1610.10363961737025736No Hit
TTGCTAGGGGATCTTTCCTAGGGGAGAGATAAGAGTTGAAAGGAAATAATTC1600.10299589303876507No Hit
TTATGTGGGAGAGGCCTCATTTTAACTGAATCACTCCTTTAAAGATCTTATC1600.10299589303876507No Hit
ATACTGGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCT1590.1023521687072728No Hit
ACAGCAGGGTCACGCTGGGAGCTGTAGACCGGAGCTGTTCCTATTCGGCCAT1580.10170844437578051No Hit
CACGAAGGGATTCTTCGAGTGACAGCTGAAGACAAGGGTACAGGGAACAAAA1580.10170844437578051No Hit
TCATCAGGGAAATCCATTTAATAACCTTTCCTGTGTGATTTATTTCACTTGG1580.10170844437578051No Hit
TGCTATGGGGTTGAAGAATAAGGTAAGAATAAAACAAGGCTTTGGAAGAAAG1570.10106472004428822No Hit
AATATGGGGAGGGGAGCTGAATATGTATCTGCAAGAGAGTGGATGAGGATTT1570.10106472004428822No Hit
TATCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1570.10106472004428822No Hit
TAGTTTGGGGATCTTCGCGTGGGATGGGAAACCATTTATGCCAAGCTTTTCC1570.10106472004428822No Hit
TTTCTTGGGCTGGCGAACTCCTGGCGCTATTCATCTGCTTTTTGTAACAAGC1570.10106472004428822No Hit
GCTATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1570.10106472004428822No Hit
GTTTTGGGGCAAGCCCAAGTACATACTTTTATACAGTGTTTGTTGTGTTAAC1570.10106472004428822No Hit
CTGATCGGGCAACAAAGTTAGTATTTCAAAACAAAACAAAAGCCCTAATCCC1560.10042099571279596No Hit
TCGTGCGGGATATAGATATAGTACTAGCTTCATTACTGAAATTTTAAAATGT1560.10042099571279596No Hit
TCTCATGGGATCTCAGGCGCATGACAACAACAAAATCAAACTCTGATAACCA1560.10042099571279596No Hit
ACGCCCGGGGGGGACTAGCCGGGTTGTATTTGGAAACGCGGAGTGAGTTTTT1560.10042099571279596No Hit
TACTATGGGATTCTCTGGGCTCACATCTCTGCCAAATATAAAGTGATTTGAT1560.10042099571279596No Hit
TTAGTCGGGGTGGAGTAAAAATTCAATATCTTTTCTAAAAACTTTTTGCATA1560.10042099571279596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)