FastQCFastQC Report
Sat 31 Dec 2022
SRR5848620.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848620.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155489
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT9420.6058306375370605TruSeq Adapter, Index 10 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC9020.5801053450726418No Hit
ATCGCTGGGCACAAAAAGTAGAAAGTAAGAAATTGTATCATACCACCAGAGA2590.16657126870711111No Hit
GAACGAGGGAGTTGCTCTGGGATTGAGCCTAAGAAATTTTTTAAAGTTCTAC2350.1511360932284599No Hit
TTATTGGGGATTTTTGGAGGGATGATGTGGAGCTTAAATTTGGATGTGTGAA2010.129269594633704No Hit
TGTTGTGGGACATTCTAGATAGCAAAGGGGCACATTATCTCAGAATCCAAGA1990.12798333001048304No Hit
GATACTGGGAGGAAGAGGAAGAAGAAGAGGAAGAAGAAGAGGAAGAAGAAGA1980.1273401976988726No Hit
TAGTTGGGCAACTCGAACTGCTTTCGTCCATGTTTCTCACATTGCTGTATTT1970.12669706538726214No Hit
AAACACGGGGTGTTGTGAATAGATTTTATTATAAATCAGAGAACTGTTTTCT1960.12605393307565166No Hit
GACAAGGGCGTTTCAGGGCTATTAGGCTAGTGAAGAAGGCTGGAGAGGGCAG1950.12541080076404118No Hit
TTGTAAGGGATGGCACTTAACTATTTGAAAAATAAATCAGTTAAATTTTAAA1910.12283827151759932No Hit
ATAGCCGGGTAATGGAAATCTTACCACCTTTCATTTTCCCTCTGTTACCAAA1910.12283827151759932No Hit
AATTTCGGGTCAAAAAGATAACCTACCATGATCAAGTGGATTTCATACCAGG1900.12219513920598885No Hit
ACTGAGGGGTGAAGGTCGTCACCTGGTTGGCCACGCCCAGCTCCACCGTGGC1890.12155200689437837No Hit
TAACACGGGAGAATGATTTCTTATGGTTTATTTTGATGAAAGTTCTTTTAAC1880.12090887458276792No Hit
ACACAGGGGCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA1830.11769321302471558No Hit
ATTAAAGGGGATGATGCTTTTTCTTGATGCTAAGATCACTGCAGAAGAATAA1820.11705008071310512No Hit
TTTCTTGGGCTGGCGAACTCCTGGCGCTATTCATCTGCTTTTTGTAACAAGC1820.11705008071310512No Hit
TTACATGGGAAGCTAGATTTCCGACAGCATTATTCTGAGAGCTGGCTACCAT1800.11576381608988416No Hit
TTAGTCGGGGTGGAGTAAAAATTCAATATCTTTTCTAAAAACTTTTTGCATA1800.11576381608988416No Hit
ATACATGGGTAGTCATTGACAGGTAAGAAGTTACCCTTTGCTTTGGTTTCCA1800.11576381608988416No Hit
AATATGGGGAGGGGAGCTGAATATGTATCTGCAAGAGAGTGGATGAGGATTT1790.1151206837782737No Hit
ATGCGAGGGGGCGTATGTGAGGTTAACCTCGTGTAATGTGAGATGATTGCCC1770.11383441915505277No Hit
CAATTCGGGTGATCCACGTTTTTGTTTTTTTAATGTTAAATGTGTAACTCAG1770.11383441915505277No Hit
CGCAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1760.1131912868434423No Hit
AAAACAGGGACTCGCCGCAGGCCCGGAAAAAGAATCCGCCTCGCAGCGTCCT1740.11190502222022136No Hit
TTACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1740.11190502222022136No Hit
TTTCGCGGGGCCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCC1740.11190502222022136No Hit
TGTTATGGGAACTATGTGAAAGAAATTGAAAATTTGAAAAATGAGTTGATGG1730.11126188990861091No Hit
TCGTGAGGGACCTAATGTCAGATCTTGAGTCCTGGGCTCCATTTTGCTCTTT1730.11126188990861091No Hit
GGTCTTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1720.11061875759700043No Hit
CACGAAGGGATTCTTCGAGTGACAGCTGAAGACAAGGGTACAGGGAACAAAA1710.10997562528538996No Hit
GCATAAGGGACCCTCCAGGGACTTAGTTTAATTTTACGAAGCCCAGCTATAA1710.10997562528538996No Hit
ACGCCCGGGGGGGACTAGCCGGGTTGTATTTGGAAACGCGGAGTGAGTTTTT1700.10933249297377949No Hit
CTTCACCGGGGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACCGGAGCGG1700.10933249297377949No Hit
GCAACAGGGTCTAGCCAAGGGGGATCTCAACGAAGGCAAACACCTTTACACG1700.10933249297377949No Hit
GCGCTGGGGCTGGATTCTAATTGTATATCCAAATATTTATTCAATAACTATT1690.10868936066216901No Hit
GTGAACGGGGTTCTTTTTACCAATAATAATTTATCAAAATAAGAGGGAAAGT1690.10868936066216901No Hit
AAGCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1690.10868936066216901No Hit
TTCTATGGGTCAATGAATTTTTATAAGTAAACACACTTGATTCAAGAACATT1690.10868936066216901No Hit
CCAAAAGGGATATAACATGTTTTCACTTGAGGGCTTAACAACTTGAGTGGTT1680.10804622835055856No Hit
TGCAGAGGGAGCATCGGCAAATGTGGAAAATGAGACGACTTATAAAGGTGAG1680.10804622835055856No Hit
GTCGCAGGGCTGAGTAGGAATATGAAAATGACACCTTTTCCAAATATTAAAT1680.10804622835055856No Hit
GCTTAGGGAGAGTCAAGATAAAAGAGTGGGAAAATATATATCATGCAAATGG1670.1074030960389481No Hit
CACTGAGGGTGATCACTTTGAGGAGCCAAAGGAAAGAGTGAAAGATTGGGAC1670.1074030960389481No Hit
ACACCAGGGATAGTTTTAAAATTAATCTTTGCATGAAGGTTGGTTTCGCTTT1670.1074030960389481No Hit
TGTTTTGGGAAATAATGAAGCGTATGGTAAATTTGAGGATTCTGTTAGAATC1660.10675996372733762No Hit
TTTCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1660.10675996372733762No Hit
TCTTAAGGGGGATGAATGGTGACCGCATCGACTCCCTGCTGGAGAACGACCG1660.10675996372733762No Hit
ACTCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1660.10675996372733762No Hit
AGTATGGGGGGGCGGGTGCTGCTTGCTGCAGGCTCTGGGGAGTCGCCATGCC1660.10675996372733762No Hit
TCGTTGGGGCGATCTGAATGTGGGGAGACCAGCCTGGTCAACATGGTGAAAC1650.10611683141572716No Hit
GTTTGCGGGATACCATTTGACCCAGCCATCCCATTACTGAGTATATACCCAA1650.10611683141572716No Hit
CAGCACGGGATTTTAAAACAGTGTCTGGTACATAGTAAGTACTAAATAGGTA1650.10611683141572716No Hit
CTAAGTGGGATTACTAGACATTAAGCTAAAAAGGATTATTCAGATTTAAAAG1650.10611683141572716No Hit
GAATGCGGGGTTCTCTGGTAAAGAAATTCCTTCAACTTTGTTTAACCTCATG1650.10611683141572716No Hit
CGAACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1650.10611683141572716No Hit
TAAGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1640.10547369910411668No Hit
CGGCACGGGATGTGTGCACAGTTTATACAAAAAGGCCCTCATGGAAGGCTGG1640.10547369910411668No Hit
AGCCTTGGGGGAAAATGAGGATGATTGAGGCTGGGTATTATGCACAAGTTTA1640.10547369910411668No Hit
AGAATTGGGAATACCTAGGTTTTAAAAATCTATCTAAAAATAAATCTACGTA1630.10483056679250623No Hit
TAGTTTGGGGATCTTCGCGTGGGATGGGAAACCATTTATGCCAAGCTTTTCC1630.10483056679250623No Hit
ATAAAGGGAGGAATTTTGGAATAGACTCAACAGGGAGGGGGTTTAGAAGCTG1630.10483056679250623No Hit
CGCACAGGGGACACCAGCAAGTCAGGCCTCTGGAACTTCTGATTGACCAGCT1630.10483056679250623No Hit
GAGCATGGGGACAAAGGGGATCCTTTCGCATTGCAGTTGCAACTTTCTTCCC1620.10418743448089576No Hit
GCTAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1620.10418743448089576No Hit
AGTGACGGGTATATAAGCTATAACATCAAATCAGGGAAAAATGAGGAAAAAA1610.10354430216928528No Hit
TAGGCTAGGGACATGGACATTTTATAGGCAGAAAGTGGATCCAAATTTTGAA1610.10354430216928528No Hit
GTCGAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1610.10354430216928528No Hit
CGAACGGGGAATTGTATTAATTAGATAATGGTTAAATTTTTATTTTTTTATC1600.1029011698576748No Hit
TTCAGGGGCAAGCAGAATGATGTAATTTAAAATAATACCATCAATTTTCATT1600.1029011698576748No Hit
CTTTCAGGGGTGGTCACAAGCGCCGGCTCCCTCCCTGCAGATGGAAGGGCGA1600.1029011698576748No Hit
TCTTCAGGGGTAAGGTGCTGTAATCTAGAATGAATCAGCTCAACTGTTTTCA1590.10225803754606436No Hit
GACACCGGGTGAGAAGCTACTAGGGAAGACTATTTGTAAAATAGTAAGGACT1590.10225803754606436No Hit
ATTTGTGGGAATCAAGATTCTGGAGAGTCTTGAACACAGAATAACGATTGTG1580.10161490523445389No Hit
CTGGTATGGGGCTGTTTGCCAAAGGAGAAAGGACTGATCATAGGTTTCTCAG1580.10161490523445389No Hit
CGGTGGGGGATTTCTTAAAGGTTGTATCTAGTTCTATTGTAGACAAGTACAT1570.10097177292284341No Hit
TTATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1570.10097177292284341No Hit
GTCTTTGGGCCGACTGTACTTTCTTTAAAGTGTTCAAAAGTATTACTAGCAA1560.10032864061123295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)