FastQCFastQC Report
Sat 31 Dec 2022
SRR5848619.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848619.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152563
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT9010.5905756965974712TruSeq Adapter, Index 10 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC8630.5656679535667233No Hit
ATCGCTGGGCACAAAAAGTAGAAAGTAAGAAATTGTATCATACCACCAGAGA2750.1802534035119918No Hit
GCATAAGGGACCCTCCAGGGACTTAGTTTAATTTTACGAAGCCCAGCTATAA1990.13043791745049588No Hit
GACAAGGGCGTTTCAGGGCTATTAGGCTAGTGAAGAAGGCTGGAGAGGGCAG1950.12781604976304872No Hit
GCTAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1930.12650511591932515No Hit
ACACAGGGGCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA1920.12584964899746334No Hit
ATTAAAGGGGATGATGCTTTTTCTTGATGCTAAGATCACTGCAGAAGAATAA1920.12584964899746334No Hit
TTATGTGGGAGAGGCCTCATTTTAACTGAATCACTCCTTTAAAGATCTTATC1890.12388324823187798No Hit
TGCAGAGGGAGCATCGGCAAATGTGGAAAATGAGACGACTTATAAAGGTGAG1850.12126138054443082No Hit
AAACACGGGGTGTTGTGAATAGATTTTATTATAAATCAGAGAACTGTTTTCT1840.12060591362256905No Hit
TAGTTGGGCAACTCGAACTGCTTTCGTCCATGTTTCTCACATTGCTGTATTT1810.11863951285698367No Hit
GATACTGGGAGGAAGAGGAAGAAGAAGAGGAAGAAGAAGAGGAAGAAGAAGA1800.11798404593512189No Hit
ACTCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1780.11667311209139831No Hit
GCTTAGGGAGAGTCAAGATAAAAGAGTGGGAAAATATATATCATGCAAATGG1760.11536217824767474No Hit
ATGCTTGGGTCTAAGTAACAGTCTATCCTTAAAGTCCACAGTCTTAACCACT1760.11536217824767474No Hit
AGCAAAGGGAGATGCACCTAAATAAGACTTGGGGGTTTTAGGAAAGGTTTTC1750.11470671132581295No Hit
GAACGAGGGAGTTGCTCTGGGATTGAGCCTAAGAAATTTTTTAAAGTTCTAC1750.11470671132581295No Hit
TTATTGGGGATTTTTGGAGGGATGATGTGGAGCTTAAATTTGGATGTGTGAA1740.11405124440395115No Hit
ACGCCCGGGGGGGACTAGCCGGGTTGTATTTGGAAACGCGGAGTGAGTTTTT1730.11339577748208937No Hit
ACTGAGGGGTGAAGGTCGTCACCTGGTTGGCCACGCCCAGCTCCACCGTGGC1730.11339577748208937No Hit
CTGGTATGGGGCTGTTTGCCAAAGGAGAAAGGACTGATCATAGGTTTCTCAG1720.11274031056022757No Hit
CACTGAGGGTGATCACTTTGAGGAGCCAAAGGAAAGAGTGAAAGATTGGGAC1710.1120848436383658No Hit
CGGCACGGGATGTGTGCACAGTTTATACAAAAAGGCCCTCATGGAAGGCTGG1710.1120848436383658No Hit
CTTCACCGGGGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACCGGAGCGG1710.1120848436383658No Hit
GCCAACGGGGACCAGCTAAGGTCATAAAGTGCAGGAGGTCTCAAAGGTCTCT1680.11011844287278041No Hit
TCTTCAGGGGTAAGGTGCTGTAATCTAGAATGAATCAGCTCAACTGTTTTCA1680.11011844287278041No Hit
AATCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1680.11011844287278041No Hit
GTGAACGGGGTTCTTTTTACCAATAATAATTTATCAAAATAAGAGGGAAAGT1670.10946297595091864No Hit
GCGATGGGGAAGTGCCTTCTCCACGGTGCAATTCAGACTTCAGTGATCTCCA1670.10946297595091864No Hit
AATTTCGGGTCAAAAAGATAACCTACCATGATCAAGTGGATTTCATACCAGG1660.10880750902905684No Hit
TTTGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1650.10815204210719506No Hit
CTAAGTGGGATTACTAGACATTAAGCTAAAAAGGATTATTCAGATTTAAAAG1650.10815204210719506No Hit
TTGTAAGGGATGGCACTTAACTATTTGAAAAATAAATCAGTTAAATTTTAAA1640.10749657518533326No Hit
TTTCTTGGGCTGGCGAACTCCTGGCGCTATTCATCTGCTTTTTGTAACAAGC1630.10684110826347148No Hit
TCGTGAGGGACCTAATGTCAGATCTTGAGTCCTGGGCTCCATTTTGCTCTTT1620.10618564134160971No Hit
AATATGGGGAGGGGAGCTGAATATGTATCTGCAAGAGAGTGGATGAGGATTT1610.10553017441974791No Hit
GTCGCAGGGCTGAGTAGGAATATGAAAATGACACCTTTTCCAAATATTAAAT1610.10553017441974791No Hit
CGCAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1600.10487470749788613No Hit
ATAGCAGGGATTACATAAAAACTAGCCATGCCGGATCACCTGAGGTCAGGAG1600.10487470749788613No Hit
GAGGCAGGGAATTGCTTAAACCCTTAAACCCGGGAGGTGGAGGTTGCAGTGA1590.10421924057602433No Hit
ATAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1580.10356377365416256No Hit
CTCGCGGGGATCTCTTGATGGGGGAGAAGAGAAAAGGATGCTTCAGAGGAAA1580.10356377365416256No Hit
CGCACAGGGGACACCAGCAAGTCAGGCCTCTGGAACTTCTGATTGACCAGCT1580.10356377365416256No Hit
CAGCACGGGATTTTAAAACAGTGTCTGGTACATAGTAAGTACTAAATAGGTA1570.10290830673230075No Hit
TAACACGGGAGAATGATTTCTTATGGTTTATTTTGATGAAAGTTCTTTTAAC1570.10290830673230075No Hit
GCTACCGGGAACTTTAAACAGAATTACTATTCGGCTCAGCTATTCCATTGGG1570.10290830673230075No Hit
CTTTCAGGGGTGGTCACAAGCGCCGGCTCCCTCCCTGCAGATGGAAGGGCGA1570.10290830673230075No Hit
GTCGAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1570.10290830673230075No Hit
GCATAGAAATGTGAACCCCTGCCACTGAGGAAGATGAAGGTAGACCCTGTGT1560.10225283981043898No Hit
TTAAGTGGGACCTGTGGTAACGGTTAAATGAATTTTAAGACACAGTCACCTT1560.10225283981043898No Hit
GCTTTAGGGGAATATATTACCATGTGAATAATAGAGACTGTGTTGCTCTCTA1560.10225283981043898No Hit
GTGCCAGGGGAAAGCTTAACTGCTGTGGTTTAAAGTACAGTTTCTCTAAAGA1540.10094190596671539No Hit
CGAACGGGGAATTGTATTAATTAGATAATGGTTAAATTTTTATTTTTTTATC1540.10094190596671539No Hit
TCAACAGGGTCACACTTTCCTAATATAATAGGACCTCTTTGGTAGATGATTG1540.10094190596671539No Hit
CTTAATGGGGTATGTCTTCCATCCTTGCTAAGATAGATGGGAGAAAGACAAA1540.10094190596671539No Hit
CAATGTGGGCAGAAGGTAAATCAGTAGATCACCCATGTGTATCTGCACCTTC1530.1002864390448536No Hit
CAACCAGGGAAGAGATAATCCCTTCAAGTTAAATTGAAAATTTTCCTGAAAC1530.1002864390448536No Hit
GAGTACGGGGGCGCGCAGCCAGGTGCGCTCCTTGGGGCTGCTGGCTGCTGCG1530.1002864390448536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)