FastQCFastQC Report
Sat 31 Dec 2022
SRR5848618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168018
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC6020.3582949445892702No Hit
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT5920.3523432013236677TruSeq Adapter, Index 10 (95% over 21bp)
CTTGCGGGTGGACGGCAGTGTTTGATGAGCCAGCGAACTCAACGCCCACAGC2540.15117427894630336No Hit
TCTATGCGGGACTGCCAGAAATTGTTTGTTTCAGAGGGGATTGGATTATCAA2460.14641288433382138No Hit
ACTTTCCCAGTTTGCAGATTAGGTAGGGAATACAAAAGGAAGCGCAGATTTA2450.14581771000726113No Hit
AGCTTAGGGGGTCACAGTGAAAGGGATTACTCTCCATGATCTAACATTCAAA2420.14403218702758036No Hit
GTTTGGGGCAGGCGAGCATGGAGAAGGCGAGCATGGAGGAGACAAGCACGAG2400.14284183837445988No Hit
CTAAGCAGGGAATCAGAGAAAAAGTCTTTAACTCCACCATTAGCACCCAAAG2260.1345093978026164No Hit
GTAATAGGGCTTCTGATAAAATTGTTTGGAAGGTCACGACCTCGCAAAAACT2260.1345093978026164No Hit
CAGAATGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG2220.13212870049637537No Hit
TGCACTGGGAGACTTCCGATTATATGGCAAATAGCATCATCTTGAATTTTGT2170.12915282886357415No Hit
ACGTTAGGGGCAAACATCAGAGAAAAAAACATTTTCTCACCAAAGGTGTTCA2140.1273673058838934No Hit
ACACAGGGGTGGAGGGAAGAGGGGCCTTGTGCTAGGTACATGGATTTACTAG2130.12677213155733313No Hit
TAAGTGGGAGTGTGACTAGATATCAATCAGTAATTAACATATCAAGGAGCTC2130.12677213155733313No Hit
ACTAAGGGGGGTAGGCAACAGGTTAATCTTATAGTCATTATATTCTCAGTGT2120.1261769572307729No Hit
ATTACGGGGGGCGCCCACGTGGCCTCCGCGGGCCCCGCCAGAGCCTGCGCCC2110.12558178290421265No Hit
GTCTTAGGGAAAGAAAGGAGTAATCAGGACTTTCTCAGAAAAACAAAAAGGC2110.12558178290421265No Hit
ATCGCTGGGATTATCAAGAAGCTAGGAGGAAGTTTGGCAGATTCCTATTTAG2110.12558178290421265No Hit
AGTCCAGGGATTTAATTTGCTCCCAACTGATTGTCACTTAAATGAAAATTTA2110.12558178290421265No Hit
TCTATAGGGAAGACGAAACTCTGCAACTTGGCAACGGTGGCCAGTTTTCGTA2110.12558178290421265No Hit
TATGGTGGGAATCCTATACTACTTTATTTATTTTGTTGCTCAAATTTTTCTA2100.1249866085776524No Hit
AGGGTTGGGAACATTTACAGTATCTTCAAAAACACTAAGTGCTCAGGGACAA2050.12201073694485115No Hit
GTGCGGGGATGATGAAAAGCTGTTACTGGTGATTATAGATGAGTATTTCCAG2040.12141556261829088No Hit
CACTGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2030.12082038829173064No Hit
AAGGAGGGATATGTATTTGCATCTGAAACTGTCACCACAATCAAAATCATAA2030.12082038829173064No Hit
ACCAGGGATTTCAATAAGATTAAAAATTTTAGCATGATCTGATAATTCTAAA2010.11963003963861014No Hit
AGCAATGGGGTGCACTGCAGTGATGGTGCGGGGAGGACCGGCACCTACATCC2010.11963003963861014No Hit
CAACCAGGGGAAATGCTTACAACATAATAAAGGGAGAAAGATGAATAAACCA2000.1190348653120499No Hit
GTTCGGGGAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGCTC2000.1190348653120499No Hit
GCTTGGGGGATTTTGAAGACACGTGGTACCACTCTCTTACTGAAAGCGCTGG1990.11843969098548966No Hit
GGTAGGTCTTCATTGGAGCCTATGAATTTTATTTCTAACAAGATCCAGTAGA1990.11843969098548966No Hit
GCGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC1990.11843969098548966No Hit
TCTGTTGGGTTGGAAGAAATGACTGCTATGTCAGGGGCACAGATAGTCACAT1970.11724934233236915No Hit
CCGACTGGGGTGGGAAGTTGACCAGGTACAGCCAATATTGCAGTCGAGTTGA1970.11724934233236915No Hit
TGATGGGGGATTCTAGCTAACTGCATTTTTCACTTTTTAAACCTGTTGAAAA1960.1166541680058089No Hit
AAATACGGGGGTTCAGAATTGAATTAGACTTCTTGTGACTGTGATGTTTGGT1940.11546381935268842No Hit
TAAGTTGGGCGGGGAGACAGAGTGCCAGCCAGCAACCCTCCCAGGTTTGTAG1940.11546381935268842No Hit
GATGCTGGGCAATTAAGACACTGGTTTTCCTTTCCGTGGAGAAATCTGGTGG1910.11367829637300765No Hit
CTTTTTGGGATATCAGTAGTTCTGAGGTATTGATAGCTTGCTTTATTTCTGC1910.11367829637300765No Hit
CTATGAGGGGATTGTTATGTTTTTATTTGGGCAAGTTTAATCATGGAAAACT1890.11248794771988717No Hit
TTACAAGGGAGGGTTTGGGTGAAAATTAAACTTAAAAAGGATTATAAAGTGC1870.11129759906676666No Hit
CGCCTAGGGGTCAACATCTAGAGGTACAGACTGTCAGATAGATGATCTATTG1870.11129759906676666No Hit
TCCTTGGGGTACCCGGAAGCGATTTCATAGCCACGGTGACTGTCCCACCCAT1870.11129759906676666No Hit
TTCTCGGGGCGATCTATAACAGGGGATCAGCCTAAATAAACTTATTGTCGTT1870.11129759906676666No Hit
AGTCCTGGGCACTGAGGTCTGTTAGATGAAAGGATATGAGAAAGGTAATATT1870.11129759906676666No Hit
ATCGGTGGGGGTCAGGCAACTTGAGGAAACTCACGATCTGCAGACGTTCTGC1870.11129759906676666No Hit
ACCATAGGGCAACTCAGAGTGACTGCTACTGAAGGAAGAGACCATGATAAAT1860.11070242474020639No Hit
TGACAAGGGTAGACTCTGGGAGAGAAAATTAACCAAGTGCTTCAGAACAGGT1860.11070242474020639No Hit
GGTTCTGGGGCAGCATGGGGAATCAAAGGTGGCATGCCAAGTTCAACTCAGG1860.11070242474020639No Hit
CTATCAGGGATATTATAAGCAGGTATTACACATACAGAATTTTCAACATTTA1860.11070242474020639No Hit
TAAGCCGGGGTCCATTAATACTTCCTTATAAAAATTCTAGTCTGTTTCATTA1850.11010725041364616No Hit
CACTGCGGGGGTATTCAGAATATAGAAAGGATTCCTGGGAATCAGTAAGAAA1850.11010725041364616No Hit
ATCCATGGATTCAGCAGTTGATGAACATCTAGATTGTTTCCATTTTGGGGTT1850.11010725041364616No Hit
CTTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1850.11010725041364616No Hit
AAGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1850.11010725041364616No Hit
TCTGGCGGGATCTGGTCACAGAATTATTCCATATTGATTGTTGTTGTGTTGT1850.11010725041364616No Hit
TACACTGGGTTAAGGAATTCAGAGAAGGGAATGTACCAAATAAGCAACAGGG1840.10951207608708591No Hit
CATCAGGGACCCACGCTTGTAAAAGACATAAGAGTTTATATTAAGGACCAAA1840.10951207608708591No Hit
CTACAGGGGTCCGATATGCAATACTTTCAAATTTCCGCTCTTGTCTCATAAA1840.10951207608708591No Hit
CTGAAGGGATTGATGGGTGTGTAGAAAAAGGAGATTCTGACACATGCTGGTA1830.10891690176052565No Hit
CTCTTGGGAGCCAAGTGAGCCCTGTTCTGGTATTTTGAATCATTAGAGAAAA1820.10832172743396541No Hit
GTCTGGGGGGATATGACAGCTTCAGAAAAAGAAATACAGAGTCCTTGGTATA1810.10772655310740514No Hit
TGATGGGCGAGTGGTGAATGCATTTCGTAGTTCTTTATATGAAGGGTTAGAA1800.1071313787808449No Hit
TCTTTGGGGGGGTGGCTGAAGTGATTTGTGCGGCCAGCACAGCTGCCTTCTT1800.1071313787808449No Hit
GGACATGGGCTGTCTCTGCAGGTAACCATCCTATAAGCAGCCTAATTTTTGA1800.1071313787808449No Hit
GTACCAGGGCCTCCTTTGTTACACACTCAAGAATTTGTCAGCACACAATTAA1800.1071313787808449No Hit
GACTTTGGGATGAAGAACTGGTACCAATCTTACTTAAAGTATTTCAAAAACT1800.1071313787808449No Hit
GTCGTGCGGGGCTTCCTTTGGGAGCTCATTCTTTTCGCCAAGGCTCACATGC1800.1071313787808449No Hit
TGTTGGGGTAAAATATTGCATAATATTGATGGGTTCAAAAACCATTAAAATA1790.10653620445428466No Hit
ATCGTTGGGATATGGATGTATTTTGATTTTTTCAAAGAATCAGTTCTGTTAC1790.10653620445428466No Hit
AAACCGGGGACTGGCTAAGGACGATCAGCTGAAGGTTCATGGGTTTTAAGTG1790.10653620445428466No Hit
TCTGGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1790.10653620445428466No Hit
TCGGTTGGGCAGCCTTCGACGTCAATAACAAGGACGTGTCGGTGATGATGAG1790.10653620445428466No Hit
CGTGACGGGGTTGCCTTAGATTAACTTACCTAGTCAGACCCAGAAGAACTTC1780.1059410301277244No Hit
GCTTCAGGGTCTAGTCCTGAAATTTCCTTAGGCTGCTGAGACAAAAACAAAA1780.1059410301277244No Hit
TATCAAGGGAATAAAAGTAAACAAATCAGCATCTTTTTCCCCTTCCCTGCTT1780.1059410301277244No Hit
CACCTGGGATAGAAATCAGGGTATACCATCTTTTATAATTTCAGCCCCCAGC1770.10534585580116416No Hit
ACACAAGGGGGTTGTTAATCTACTGTATATTTTTGTTTGGTATATTTACTAA1770.10534585580116416No Hit
CATTCAGGGACAGGGACAATATCATAATCATCTTTGTATCCCCAATACCCAT1770.10534585580116416No Hit
AAGCGAGGGGAATGTGGTTCGCTTCACCGGGTCCTCTTGGATCAAACATGGC1770.10534585580116416No Hit
GATGCCGGGGATATTGTAACAGAAAAATGATAATATTGTTTGATTATATGAA1770.10534585580116416No Hit
TACCTTGGGGACCTGATGAGTGTTTTAATGTTTCCCGGGTAATTCCAATGGT1760.10475068147460392No Hit
AGTCTCGGGATATAGGAGAAAGAAAAGACAAGACCGTTCTGGAAAGGAAAAT1760.10475068147460392No Hit
GCTGCCGGGATCATCTAGGTTCCCTCCCCTTGCCTCTCACCCCTCAACAGGC1750.10415550714804366No Hit
TCAAATGGGCCTGGGCTTGAGCTTTAAACTTTATACTAAAAGTTAGATTTCC1750.10415550714804366No Hit
TGTGTTGGGGGGGCTCTTGGGTTCTCAGAGCTTGTTTTCTATGTTGGTTACA1750.10415550714804366No Hit
TTTGTTGGGATTCTTCTTAGATTTTTGGAGGCAAAATTTAGACCAGTATACT1740.10356033282148341No Hit
ATAAAAGGGACAGGTCGGGAGGCCCAGGGAAGACAAGAAGAGGCTGAAGTGG1740.10356033282148341No Hit
TATTTAGGGATTGTATTTGAATAAAATTGATGTCACCTAGAATTTTGAAAAG1740.10356033282148341No Hit
GTATTTGGGGGATGCAGTGCTCGCTGTTGCTGAATAGGCGATCACAACGTGC1740.10356033282148341No Hit
GTCAGAGGGGCACTAAAAGTGATGTGATTTATGCATTTATGCATGAGAACTA1740.10356033282148341No Hit
GCCATAGGGAGTTGCAGAATATTAGATTTATGACATCTAGCCCAGAGTATTT1740.10356033282148341No Hit
GACATAGGGCTGTTCCAGTTTTGCTGGATTTGTACATAGCCATTCTAGAAAT1740.10356033282148341No Hit
CTTACAGGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAA1730.10296515849492316No Hit
GGTTGCGGGTCCCGAGACCAGTTGGGTACCTCTCTATGCGAACCAGAGACAT1730.10296515849492316No Hit
CAAAGTGGAGGAAGGCACTGTTGAGTTATCTGCCTAGTGGAAATGAGCCACC1730.10296515849492316No Hit
TTCGTCGGGACTCCTGTAAATTAATCTACAGTCAGGCTAGTATGATTTTAAT1730.10296515849492316No Hit
AGTTTAGGGATTCCAAAACACTATAAACATGTAATGGTATTAGTGAACTGAA1730.10296515849492316No Hit
TAATGCGGGCAGCTTACTCAAAGGTCCTCAGGGATATCTCTTCCACGCTCAA1730.10296515849492316No Hit
TGTCCAGGGGATGGTAATGGCACAATTGACTTCCCTGAATTTCTGACAATGA1720.10236998416836292No Hit
TTAACAGGGCTGGCAGATCCTCCTGTCTGGAGGTACCACCTTCTATTCTGGT1720.10236998416836292No Hit
ATAAGAGGGAATTGCAATCATGGGCATTTTAATGTATTTTAAAATAAATTTC1720.10236998416836292No Hit
AATAACGGGGTAAATAAGAGATTTGGAGCACTCTGAGTTTACCATTTGTAAT1710.10177480984180268No Hit
GTATGGGGGACAGTTTAAAAAATGAGAGTTTTGAAAAGCCAGGGATGCTCTC1710.10177480984180268No Hit
AAGAAAGGGAGTGTCTTTTGGAAAACTACTTAAAGGGTGATTTTCTGTTACT1700.10117963551524241No Hit
ATAGATGGGATGACACTGACACTGCTATTTTGAAACCTGGAGGATGGAAAGG1700.10117963551524241No Hit
CTGATAGGGGACACCTGGCATTCCTCTCTGTTCATGGAGCTTCTTTGAGGCT1700.10117963551524241No Hit
ACTGCGGGGCAGGAATTTAAATGGAGAGAGAAGTGCATTTTTACTGAACCTT1700.10117963551524241No Hit
CACTCCGGGGCCCGAGCTGTGCGGTAGGCATTGAGGCAGCCAGCGCAGGGGC1690.10058446118868217No Hit
GTTGTGGGGAATTTGTCTCTCTCACAAGTAACATCATCTAGTATATAAGACT1690.10058446118868217No Hit
TGTCATGGGATGCCAATTTCTCTACTTGCTTGCTCTTTTTTTTCCTTTCTTT1690.10058446118868217No Hit
TGAGGTGGGATTTCAAAGTAATCCTGAAAAGCTAATTCAGGCCATGATGGGA1690.10058446118868217No Hit
TGGAGCGGGAAGCTAGGGAGGGGACTGAAGAATTTTTTTTCCTGTTTGTTTT1690.10058446118868217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)