FastQCFastQC Report
Sat 31 Dec 2022
SRR5848617.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848617.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169268
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC6150.36332915849422215No Hit
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT6020.35564902994068576TruSeq Adapter, Index 10 (95% over 21bp)
GTAATAGGGCTTCTGATAAAATTGTTTGGAAGGTCACGACCTCGCAAAAACT2450.14474088427818607No Hit
GTTTGGGGCAGGCGAGCATGGAGAAGGCGAGCATGGAGGAGACAAGCACGAG2410.14237776780017486No Hit
TATGGTGGGAATCCTATACTACTTTATTTATTTTGTTGCTCAAATTTTTCTA2390.14119620956116927No Hit
TACACTGGGTTAAGGAATTCAGAGAAGGGAATGTACCAAATAAGCAACAGGG2360.13942387220266086No Hit
TAAGTGGGAGTGTGACTAGATATCAATCAGTAATTAACATATCAAGGAGCTC2330.13765153484415246No Hit
ACTTTCCCAGTTTGCAGATTAGGTAGGGAATACAAAAGGAAGCGCAGATTTA2320.13706075572464965No Hit
ATCGCTGGGATTATCAAGAAGCTAGGAGGAAGTTTGGCAGATTCCTATTTAG2310.13646997660514687No Hit
AGCTTAGGGGGTCACAGTGAAAGGGATTACTCTCCATGATCTAACATTCAAA2290.13528841836614125No Hit
ACGTTAGGGGCAAACATCAGAGAAAAAAACATTTTCTCACCAAAGGTGTTCA2280.13469763924663847No Hit
AGTCCAGGGATTTAATTTGCTCCCAACTGATTGTCACTTAAATGAAAATTTA2280.13469763924663847No Hit
CTTGCGGGTGGACGGCAGTGTTTGATGAGCCAGCGAACTCAACGCCCACAGC2280.13469763924663847No Hit
TCTATGCGGGACTGCCAGAAATTGTTTGTTTCAGAGGGGATTGGATTATCAA2250.13292530188813007No Hit
ACCAGGGATTTCAATAAGATTAAAAATTTTAGCATGATCTGATAATTCTAAA2220.13115296452962166No Hit
TATTTAGGGATTGTATTTGAATAAAATTGATGTCACCTAGAATTTTGAAAAG2210.13056218541011888No Hit
CTGAAGGGATTGATGGGTGTGTAGAAAAAGGAGATTCTGACACATGCTGGTA2190.12938062717111326No Hit
GCGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC2180.12878984805161045No Hit
TGCACTGGGAGACTTCCGATTATATGGCAAATAGCATCATCTTGAATTTTGT2160.12760828981260486No Hit
GTGGCTGGGTGTGGATATCTATGAAGACATGCTAGAAAATCAGTGTGACAAT2150.12701751069310205No Hit
GTCTTAGGGAAAGAAAGGAGTAATCAGGACTTTCTCAGAAAAACAAAAAGGC2150.12701751069310205No Hit
ATTACGGGGGGCGCCCACGTGGCCTCCGCGGGCCCCGCCAGAGCCTGCGCCC2130.12583595245409646No Hit
CACTGCGGGGGTATTCAGAATATAGAAAGGATTCCTGGGAATCAGTAAGAAA2130.12583595245409646No Hit
GGTAGGTCTTCATTGGAGCCTATGAATTTTATTTCTAACAAGATCCAGTAGA2130.12583595245409646No Hit
CACTGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2130.12583595245409646No Hit
CAGAATGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG2130.12583595245409646No Hit
GTCGTGCGGGGCTTCCTTTGGGAGCTCATTCTTTTCGCCAAGGCTCACATGC2120.12524517333459367No Hit
TCCTTGGGGTACCCGGAAGCGATTTCATAGCCACGGTGACTGTCCCACCCAT2100.12406361509558807No Hit
TCTTTGGGGGGGTGGCTGAAGTGATTTGTGCGGCCAGCACAGCTGCCTTCTT2100.12406361509558807No Hit
ATCGTTGGGATATGGATGTATTTTGATTTTTTCAAAGAATCAGTTCTGTTAC2100.12406361509558807No Hit
ATCCATGGATTCAGCAGTTGATGAACATCTAGATTGTTTCCATTTTGGGGTT2090.12347283597608527No Hit
TTCTCGGGGCGATCTATAACAGGGGATCAGCCTAAATAAACTTATTGTCGTT2080.12288205685658246No Hit
TCAAATGGGCCTGGGCTTGAGCTTTAAACTTTATACTAAAAGTTAGATTTCC2080.12288205685658246No Hit
AAGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2070.12229127773707966No Hit
GTGCGGGGATGATGAAAAGCTGTTACTGGTGATTATAGATGAGTATTTCCAG2050.12110971949807404No Hit
AGGGTTGGGAACATTTACAGTATCTTCAAAAACACTAAGTGCTCAGGGACAA2040.12051894037857125No Hit
CATCAGGGACCCACGCTTGTAAAAGACATAAGAGTTTATATTAAGGACCAAA2030.11992816125906845No Hit
AAATACGGGGGTTCAGAATTGAATTAGACTTCTTGTGACTGTGATGTTTGGT2020.11933738213956566No Hit
TTTAAAGGGGAATGGAATAGATAGCTGATAGGTAAACCCTCACATACATGGT2020.11933738213956566No Hit
AGTCTCGGGATATAGGAGAAAGAAAAGACAAGACCGTTCTGGAAAGGAAAAT2000.11815582390056005No Hit
TCTGTTGGGTTGGAAGAAATGACTGCTATGTCAGGGGCACAGATAGTCACAT1990.11756504478105725No Hit
CTACAGGGGTCCGATATGCAATACTTTCAAATTTCCGCTCTTGTCTCATAAA1980.11697426566155446No Hit
CTTGCTGGGATTCCCAGCAAGGGTTTGGCAAATTACAAAATAAGGATGATGA1980.11697426566155446No Hit
TGATGGGGGATTCTAGCTAACTGCATTTTTCACTTTTTAAACCTGTTGAAAA1970.11638348654205166No Hit
TGTCCAGGGGATGGTAATGGCACAATTGACTTCCCTGAATTTCTGACAATGA1960.11579270742254887No Hit
AGCAATGGGGTGCACTGCAGTGATGGTGCGGGGAGGACCGGCACCTACATCC1940.11461114918354326No Hit
GTCTGGGGGGATATGACAGCTTCAGAAAAAGAAATACAGAGTCCTTGGTATA1930.11402037006404046No Hit
ACTAAGGGGGGTAGGCAACAGGTTAATCTTATAGTCATTATATTCTCAGTGT1930.11402037006404046No Hit
GCTGCCGGGATCATCTAGGTTCCCTCCCCTTGCCTCTCACCCCTCAACAGGC1930.11402037006404046No Hit
CTAAGCAGGGAATCAGAGAAAAAGTCTTTAACTCCACCATTAGCACCCAAAG1930.11402037006404046No Hit
TGCAAGGGGGAAAGACTAGTTAAGAACATTTTTAAGATACTTAATAAATATG1920.11342959094453767No Hit
ACACAGGGGTGGAGGGAAGAGGGGCCTTGTGCTAGGTACATGGATTTACTAG1920.11342959094453767No Hit
TCTGGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1910.11283881182503487No Hit
TTTGCCGGGCTGCCTAAGGGAACTGACCAGAAGTTCATACTGCCAACATACT1900.11224803270553205No Hit
CACCTGGGATAGAAATCAGGGTATACCATCTTTTATAATTTCAGCCCCCAGC1890.11165725358602925No Hit
GCTTGGGGGATTTTGAAGACACGTGGTACCACTCTCTTACTGAAAGCGCTGG1890.11165725358602925No Hit
ATCGGTGGGGGTCAGGCAACTTGAGGAAACTCACGATCTGCAGACGTTCTGC1890.11165725358602925No Hit
TGACAAGGGTAGACTCTGGGAGAGAAAATTAACCAAGTGCTTCAGAACAGGT1880.11106647446652645No Hit
GCTTCAGGGTCTAGTCCTGAAATTTCCTTAGGCTGCTGAGACAAAAACAAAA1880.11106647446652645No Hit
CATTCAGGGACAGGGACAATATCATAATCATCTTTGTATCCCCAATACCCAT1880.11106647446652645No Hit
CTATCAGGGATATTATAAGCAGGTATTACACATACAGAATTTTCAACATTTA1880.11106647446652645No Hit
GATGCCGGGGATATTGTAACAGAAAAATGATAATATTGTTTGATTATATGAA1870.11047569534702364No Hit
TGAGGTGGGATTTCAAAGTAATCCTGAAAAGCTAATTCAGGCCATGATGGGA1870.11047569534702364No Hit
TATCAAGGGAATAAAAGTAAACAAATCAGCATCTTTTTCCCCTTCCCTGCTT1870.11047569534702364No Hit
GGACATGGGCTGTCTCTGCAGGTAACCATCCTATAAGCAGCCTAATTTTTGA1860.10988491622752085No Hit
AGTCCTGGGCACTGAGGTCTGTTAGATGAAAGGATATGAGAAAGGTAATATT1850.10929413710801805No Hit
AAGGAGGGATATGTATTTGCATCTGAAACTGTCACCACAATCAAAATCATAA1850.10929413710801805No Hit
ACACAAGGGGGTTGTTAATCTACTGTATATTTTTGTTTGGTATATTTACTAA1840.10870335798851526No Hit
GCAGTCGGGAGAAACTTCTGCAGCATCCAGAACTGAGTAATAGTCAACTTCT1840.10870335798851526No Hit
TCGCTTGGGGATTGTTAGACTTAATAAATCACTGTTAAATTTAAAGATGACA1840.10870335798851526No Hit
CTTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1840.10870335798851526No Hit
TGTGCAGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCG1830.10811257886901246No Hit
TAAGCCGGGGTCCATTAATACTTCCTTATAAAAATTCTAGTCTGTTTCATTA1830.10811257886901246No Hit
GACATAGGGCTGTTCCAGTTTTGCTGGATTTGTACATAGCCATTCTAGAAAT1830.10811257886901246No Hit
GTTCGGGGAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGCTC1830.10811257886901246No Hit
TGATGGGCGAGTGGTGAATGCATTTCGTAGTTCTTTATATGAAGGGTTAGAA1820.10752179974950965No Hit
CTTACAGGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAA1810.10693102063000685No Hit
GGTTGCGGGTCCCGAGACCAGTTGGGTACCTCTCTATGCGAACCAGAGACAT1810.10693102063000685No Hit
GGTTCTGGGGCAGCATGGGGAATCAAAGGTGGCATGCCAAGTTCAACTCAGG1810.10693102063000685No Hit
TCTTGTGGGACTTTCCTTAATCCCCCAGCTGACTAAAACTTTTGTTTCATCT1800.10634024151050406No Hit
AAACCGGGGACTGGCTAAGGACGATCAGCTGAAGGTTCATGGGTTTTAAGTG1800.10634024151050406No Hit
TGTCTAGGGGATGACAAAGAAGTCTGGATTTCTAGTCCAGCTGGGCAGCACA1800.10634024151050406No Hit
TTACAAGGGAGGGTTTGGGTGAAAATTAAACTTAAAAAGGATTATAAAGTGC1770.10456790415199566No Hit
CGTGACGGGGTTGCCTTAGATTAACTTACCTAGTCAGACCCAGAAGAACTTC1770.10456790415199566No Hit
ATAGATGGGATGACACTGACACTGCTATTTTGAAACCTGGAGGATGGAAAGG1770.10456790415199566No Hit
CGTGGGGGGGGGACAGCGGCAATCATGGCGCCACCTGTGAGATACTGCATCC1770.10456790415199566No Hit
GATATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1770.10456790415199566No Hit
GTTGGCGGGACGAGCCTTCAACAAGCTGCCCAGTCCTCTCCTCAGCAGACGC1770.10456790415199566No Hit
TACCACGGGACTTCTCTCTAGGTAGAGGTTCTGGGGAGCAGTGAGGTTGTGA1770.10456790415199566No Hit
GTACCAGGGCCTCCTTTGTTACACACTCAAGAATTTGTCAGCACACAATTAA1770.10456790415199566No Hit
AAAAAAAGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAG1760.10397712503249285No Hit
TCTATAGGGAAGACGAAACTCTGCAACTTGGCAACGGTGGCCAGTTTTCGTA1760.10397712503249285No Hit
TGCTTAGGGCTGATGGGTAATTTGTGGAGAAATATCTTAATTTTGATTTCCT1750.10338634591299005No Hit
CGTTAAGGGAAACTTCTTTGACTTAATATTCTTTTAAATTACAGCACATTTT1750.10338634591299005No Hit
TCTTTGGGGGGAGCACCGTTTGGTCTCACTCATAACACTTCTCAGAGATGCT1740.10279556679348724No Hit
CTTTGTGGGAGATGTGTCTCTGAGTAGTAAAGGCTTAGCCTTGTTCTGTTTT1740.10279556679348724No Hit
TAATGCGGGGGGTCAAGTGACTAAGTGCATGTGGTGGATGCCTTGGCGATTA1740.10279556679348724No Hit
GTCAGAGGGGCACTAAAAGTGATGTGATTTATGCATTTATGCATGAGAACTA1740.10279556679348724No Hit
CTTTTTGGGATATCAGTAGTTCTGAGGTATTGATAGCTTGCTTTATTTCTGC1740.10279556679348724No Hit
TGTACAGGGGCACACAATGGGACATACAGCCTAAACTCCCCCAATCCAACAC1730.10220478767398444No Hit
ACCATGGGGGAATCCAGGTGGAAATAATCCAGGAATTATGGATAGTCTTGTA1730.10220478767398444No Hit
TCGGTTGGGCAGCCTTCGACGTCAATAACAAGGACGTGTCGGTGATGATGAG1730.10220478767398444No Hit
TTTGTTGGGATTCTTCTTAGATTTTTGGAGGCAAAATTTAGACCAGTATACT1720.10161400855448165No Hit
TAACGAGGGTTGCCTTGGAAAAGAGCATGTTTGCCTTTAAAATTTTTGTTTT1720.10161400855448165No Hit
ATCCATGGGCGATCTAGGTGCGGGAAAGTTCTTGTGGAATTCAGTCACAAGG1720.10161400855448165No Hit
CCGACTGGGGTGGGAAGTTGACCAGGTACAGCCAATATTGCAGTCGAGTTGA1720.10161400855448165No Hit
CTATGAGGGGATTGTTATGTTTTTATTTGGGCAAGTTTAATCATGGAAAACT1710.10102322943497885No Hit
TCACCAGGGATACGGTTCAGAAACAATTCAACATAAAGAGGAGTGTGTGTAG1710.10102322943497885No Hit
GAAAGAGGGCGATCTAATTAGGGGTGAGCGCGTTCTTAGCAGTTGGCTTCAT1710.10102322943497885No Hit
TTACGCGGGATTATAATCAGCTTCAGAGATTCCTTAAACCTTCCTAATTTCC1700.10043245031547605No Hit
TAACCAGGGGCAGAAAAAGATCCAAACCGCTGACAAGTTTTAAAAGCTACTT1700.10043245031547605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)