FastQCFastQC Report
Sat 31 Dec 2022
SRR5848616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168335
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT6180.3671250779695251TruSeq Adapter, Index 10 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC5990.35583806100929694No Hit
GTAATAGGGCTTCTGATAAAATTGTTTGGAAGGTCACGACCTCGCAAAAACT2390.14197879228918525No Hit
ACTTTCCCAGTTTGCAGATTAGGTAGGGAATACAAAAGGAAGCGCAGATTTA2230.13247393590162473No Hit
GATGCCGGGGATATTGTAACAGAAAAATGATAATATTGTTTGATTATATGAA2220.1318798823774022No Hit
ACACAGGGGTGGAGGGAAGAGGGGCCTTGTGCTAGGTACATGGATTTACTAG2210.13128582885317966No Hit
TCTATGCGGGACTGCCAGAAATTGTTTGTTTCAGAGGGGATTGGATTATCAA2160.128315561232067No Hit
ATCGCTGGGATTATCAAGAAGCTAGGAGGAAGTTTGGCAGATTCCTATTTAG2160.128315561232067No Hit
AAATACGGGGGTTCAGAATTGAATTAGACTTCTTGTGACTGTGATGTTTGGT2140.12712745418362195No Hit
GCGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC2140.12712745418362195No Hit
TCAAATGGGCCTGGGCTTGAGCTTTAAACTTTATACTAAAAGTTAGATTTCC2130.12653340065939941No Hit
CAGAATGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG2130.12653340065939941No Hit
TGCACTGGGAGACTTCCGATTATATGGCAAATAGCATCATCTTGAATTTTGT2120.12593934713517688No Hit
AGTCTCGGGATATAGGAGAAAGAAAAGACAAGACCGTTCTGGAAAGGAAAAT2120.12593934713517688No Hit
GGTAGGTCTTCATTGGAGCCTATGAATTTTATTTCTAACAAGATCCAGTAGA2110.12534529361095437No Hit
CTATCAGGGATATTATAAGCAGGTATTACACATACAGAATTTTCAACATTTA2090.12415718656250928No Hit
CACTGCGGGGGTATTCAGAATATAGAAAGGATTCCTGGGAATCAGTAAGAAA2070.12296907951406422No Hit
GTCTTAGGGAAAGAAAGGAGTAATCAGGACTTTCTCAGAAAAACAAAAAGGC2070.12296907951406422No Hit
CTTGCGGGTGGACGGCAGTGTTTGATGAGCCAGCGAACTCAACGCCCACAGC2070.12296907951406422No Hit
GTTTGGGGCAGGCGAGCATGGAGAAGGCGAGCATGGAGGAGACAAGCACGAG2060.12237502598984168No Hit
CTAGAAGGGCAGAACTTTAGTTGTACTCTGCTTGAGGGGAAGAAGGCTCCTG2060.12237502598984168No Hit
CTAAGCAGGGAATCAGAGAAAAAGTCTTTAACTCCACCATTAGCACCCAAAG2050.12178097246561914No Hit
TCTGGCGGGATCTGGTCACAGAATTATTCCATATTGATTGTTGTTGTGTTGT2020.11999881189295154No Hit
TGTCCAGGGGATGGTAATGGCACAATTGACTTCCCTGAATTTCTGACAATGA2000.1188107048445065No Hit
TATGGTGGGAATCCTATACTACTTTATTTATTTTGTTGCTCAAATTTTTCTA2000.1188107048445065No Hit
AAGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2000.1188107048445065No Hit
AGTCCTGGGCACTGAGGTCTGTTAGATGAAAGGATATGAGAAAGGTAATATT1990.11821665132028394No Hit
ACTAAGGGGGGTAGGCAACAGGTTAATCTTATAGTCATTATATTCTCAGTGT1970.1170285442718389No Hit
ACCAGGGATTTCAATAAGATTAAAAATTTTAGCATGATCTGATAATTCTAAA1970.1170285442718389No Hit
ATCGTTGGGATATGGATGTATTTTGATTTTTTCAAAGAATCAGTTCTGTTAC1970.1170285442718389No Hit
TAAGCCGGGGTCCATTAATACTTCCTTATAAAAATTCTAGTCTGTTTCATTA1960.11643449074761636No Hit
CATTCAGGGACAGGGACAATATCATAATCATCTTTGTATCCCCAATACCCAT1950.11584043722339384No Hit
CAACCAGGGGAAATGCTTACAACATAATAAAGGGAGAAAGATGAATAAACCA1950.11584043722339384No Hit
GGTTCTGGGGCAGCATGGGGAATCAAAGGTGGCATGCCAAGTTCAACTCAGG1950.11584043722339384No Hit
GTACCAGGGCCTCCTTTGTTACACACTCAAGAATTTGTCAGCACACAATTAA1940.1152463836991713No Hit
AGTCCAGGGATTTAATTTGCTCCCAACTGATTGTCACTTAAATGAAAATTTA1940.1152463836991713No Hit
TACACTGGGTTAAGGAATTCAGAGAAGGGAATGTACCAAATAAGCAACAGGG1920.11405827665072624No Hit
TAAGTTGGGCGGGGAGACAGAGTGCCAGCCAGCAACCCTCCCAGGTTTGTAG1910.1134642231265037No Hit
TCAAAGGGGCAAGATGGCAGCATCAAAATGAAGCCTCTGATGGGGGTGATAT1910.1134642231265037No Hit
TCTATAGGGAAGACGAAACTCTGCAACTTGGCAACGGTGGCCAGTTTTCGTA1910.1134642231265037No Hit
CTGAAGGGATTGATGGGTGTGTAGAAAAAGGAGATTCTGACACATGCTGGTA1890.11227611607805862No Hit
CTTAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1890.11227611607805862No Hit
AATGACGGGTTTTTTAATGGTTTATTGCTGCAGGTTTTTTGTGTATTTGATT1880.1116820625538361No Hit
CATCAGGGACCCACGCTTGTAAAAGACATAAGAGTTTATATTAAGGACCAAA1880.1116820625538361No Hit
TCGCTTGGGGATTGTTAGACTTAATAAATCACTGTTAAATTTAAAGATGACA1880.1116820625538361No Hit
TTACAAGGGAGGGTTTGGGTGAAAATTAAACTTAAAAAGGATTATAAAGTGC1870.11108800902961356No Hit
ACCATAGGGCAACTCAGAGTGACTGCTACTGAAGGAAGAGACCATGATAAAT1870.11108800902961356No Hit
CTATGAGGGGATTGTTATGTTTTTATTTGGGCAAGTTTAATCATGGAAAACT1870.11108800902961356No Hit
TGATGGGCGAGTGGTGAATGCATTTCGTAGTTCTTTATATGAAGGGTTAGAA1870.11108800902961356No Hit
TGCAAGGGGGAAAGACTAGTTAAGAACATTTTTAAGATACTTAATAAATATG1870.11108800902961356No Hit
ATAGCTGGGTAGATCTAAAAAATCTTATTAAAATGTTATAGCAATAGTATTA1870.11108800902961356No Hit
GCTGCCGGGATCATCTAGGTTCCCTCCCCTTGCCTCTCACCCCTCAACAGGC1860.11049395550539103No Hit
GTCAGAGGGGCACTAAAAGTGATGTGATTTATGCATTTATGCATGAGAACTA1860.11049395550539103No Hit
CTACAGGGGTCCGATATGCAATACTTTCAAATTTCCGCTCTTGTCTCATAAA1860.11049395550539103No Hit
CACCTGGGATAGAAATCAGGGTATACCATCTTTTATAATTTCAGCCCCCAGC1850.1098999019811685No Hit
GCCATAGGGAGTTGCAGAATATTAGATTTATGACATCTAGCCCAGAGTATTT1850.1098999019811685No Hit
GCTTCAGGGTCTAGTCCTGAAATTTCCTTAGGCTGCTGAGACAAAAACAAAA1840.10930584845694596No Hit
TTTGTTGGGATTCTTCTTAGATTTTTGGAGGCAAAATTTAGACCAGTATACT1830.10871179493272343No Hit
AGGGTTGGGAACATTTACAGTATCTTCAAAAACACTAAGTGCTCAGGGACAA1820.10811774140850092No Hit
CTTTTTGGGATATCAGTAGTTCTGAGGTATTGATAGCTTGCTTTATTTCTGC1820.10811774140850092No Hit
AAGGAGGGATATGTATTTGCATCTGAAACTGTCACCACAATCAAAATCATAA1820.10811774140850092No Hit
TGCGCTGGGTAACAATTAAATTAAAAAACTTTTATATTTCTCTTTATTATAG1810.10752368788427838No Hit
ATTCGCGGGGAGAGCAGAAGGTGACATCTCCAATGTGGCCAATGGGTTTAAG1810.10752368788427838No Hit
TAAGTGGGAGTGTGACTAGATATCAATCAGTAATTAACATATCAAGGAGCTC1810.10752368788427838No Hit
CACTCCGGGGCCCGAGCTGTGCGGTAGGCATTGAGGCAGCCAGCGCAGGGGC1800.10692963436005583No Hit
GCTTGGGGGATTTTGAAGACACGTGGTACCACTCTCTTACTGAAAGCGCTGG1800.10692963436005583No Hit
AATAACGGGGTAAATAAGAGATTTGGAGCACTCTGAGTTTACCATTTGTAAT1800.10692963436005583No Hit
TAATGCGGGCAGCTTACTCAAAGGTCCTCAGGGATATCTCTTCCACGCTCAA1800.10692963436005583No Hit
AGCTTAGGGGGTCACAGTGAAAGGGATTACTCTCCATGATCTAACATTCAAA1800.10692963436005583No Hit
CAAAGTGGAGGAAGGCACTGTTGAGTTATCTGCCTAGTGGAAATGAGCCACC1790.10633558083583332No Hit
ACACCAGGGATTTCTTTTCTTTTAAAGTTGATGTTTATGTTGGTGATTTCGA1790.10633558083583332No Hit
TGACAAGGGTAGACTCTGGGAGAGAAAATTAACCAAGTGCTTCAGAACAGGT1780.10574152731161078No Hit
TTCTCGGGGCGATCTATAACAGGGGATCAGCCTAAATAAACTTATTGTCGTT1780.10574152731161078No Hit
ACGTTAGGGGCAAACATCAGAGAAAAAAACATTTTCTCACCAAAGGTGTTCA1780.10574152731161078No Hit
CTCTTGGGAGCCAAGTGAGCCCTGTTCTGGTATTTTGAATCATTAGAGAAAA1780.10574152731161078No Hit
CTTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1770.10514747378738824No Hit
GTTCGGGGAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGCTC1760.10455342026316572No Hit
GCTTAGGGGCTACTTGGGGATCATGCAGAGAGCTTCACGTCTGAAGAGAGAG1750.10395936673894318No Hit
TGTATAGGGTATGTATCAGTGTCCTTTCCAGAGCAACCAGAAGGAGGTTATA1750.10395936673894318No Hit
TATGCTGGGTACGCAAATCAATCATTGCGACGCATCATATCAACAGAAGAAA1740.10336531321472064No Hit
TGTCATGGGATGCCAATTTCTCTACTTGCTTGCTCTTTTTTTTCCTTTCTTT1740.10336531321472064No Hit
GCAGTCGGGAGAAACTTCTGCAGCATCCAGAACTGAGTAATAGTCAACTTCT1730.10277125969049812No Hit
CGTTAAGGGCTTCCCAGGGAGCACGGGCATGCCTTGGAATATTGCCAAGCTT1730.10277125969049812No Hit
TTTAAAGGGGAATGGAATAGATAGCTGATAGGTAAACCCTCACATACATGGT1720.10217720616627558No Hit
GGACACGGGGCCCATTTTCAATGTCTTACAAAAATGAGAAGTGTGATGGTTT1720.10217720616627558No Hit
CGGCCGGGGTCGCCTTTTGTGCCTTGTTTGACTGCGACCGCCCTGTGGCGCA1720.10217720616627558No Hit
TCGGTTGGGCAGCCTTCGACGTCAATAACAAGGACGTGTCGGTGATGATGAG1720.10217720616627558No Hit
TCCTTGGGGTACCCGGAAGCGATTTCATAGCCACGGTGACTGTCCCACCCAT1710.10158315264205305No Hit
GGTTGCGGGTCCCGAGACCAGTTGGGTACCTCTCTATGCGAACCAGAGACAT1710.10158315264205305No Hit
TTGCTGGGAGTATAGAGTTGTTTTGTTGGTTTAAGAGTAACATTCAGTAGTA1710.10158315264205305No Hit
ATAAGAGGGAATTGCAATCATGGGCATTTTAATGTATTTTAAAATAAATTTC1710.10158315264205305No Hit
TACCTTGGGGACCTGATGAGTGTTTTAATGTTTCCCGGGTAATTCCAATGGT1710.10158315264205305No Hit
GTATGGGGGACAGTTTAAAAAATGAGAGTTTTGAAAAGCCAGGGATGCTCTC1710.10158315264205305No Hit
CGGACGGGGCGCTGGAGCTATTTGGTGCTTGAATGTGACCATCCTTTTTACT1710.10158315264205305No Hit
CGCCTAGGGGTCAACATCTAGAGGTACAGACTGTCAGATAGATGATCTATTG1700.10098909911783051No Hit
TTTTATGGGGATATTATGAACAATATTTGACAACCTAGATGAAATGGACAAA1700.10098909911783051No Hit
CTGATAGGGGACACCTGGCATTCCTCTCTGTTCATGGAGCTTCTTTGAGGCT1700.10098909911783051No Hit
AAATTAGGGGTGCAGTGATTATTTTTTAAAGTCTTCTTTCATGTAAGTAGCA1700.10098909911783051No Hit
TCTGTTGGGTTGGAAGAAATGACTGCTATGTCAGGGGCACAGATAGTCACAT1690.10039504559360798No Hit
TCACAAGGGATACACCAGACAGAAAGATAACCTACTGCATACGCCAGACACA1690.10039504559360798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)