FastQCFastQC Report
Sat 31 Dec 2022
SRR5848615.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848615.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228419
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC8510.37256095158458796No Hit
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT7830.3427910988140216TruSeq Adapter, Index 10 (95% over 21bp)
AAAGCTGGGCAGATTTGGAACTTCTAGATGTGTAAGTAAAGCCATAAATATA3310.1449091362802569No Hit
TCAGTAGGGAAAGCAGAATATTAGAGCAGATTGCAGTTGGCCCACCCACCCG3280.14359576042273192No Hit
AAAATGGGTAGCATCAAGGAAGTTATGATTGGCGTGGACAAATGTTTCAGCC2980.13046200184748205No Hit
TACACTGGGCTAGTAAATGCCTCCTTAACAATTAGCCCAATTCATATCAATG2910.12739745817992373No Hit
CACAGGGGGTTACTGTAAATGGAATTGCTTTAATAATTTCCTTTTCAGATTG2900.12695966622741542No Hit
CATATAGGGACGACCGGGTGCAGGAGAAGACAGTTGTGTTTCAACAGGAGAG2830.12389512255985712No Hit
GTTCAGGGGATTTGGAAGCATTAAATCCATACTTATGGGTTTAGATTTTTTT2820.12345733060734879No Hit
CACTTAGGGGATTGTTCCTGCCCTTCCCCAACTAATCCATCCACCTTGTGAC2770.12126837084480713No Hit
TGTACAGGGTCTGTAATTTTCACTTTTAACATGTTACCTAAAGCACTTTTTG2760.1208305788922988No Hit
TGCTCGCGGGCGGATTACTTTGTTTTGTGGTATCCAGAGTTTGAAAGGATTG2760.1208305788922988No Hit
TTATCCGGGGTGCTTGTAGGATGAAATTGATAAACAATTATTTTACCAACTT2750.12039278693979048No Hit
TATTTTGGGGATGCTGTTGTATGCTCTTTTTGTTTGATACAGAAGCTCAGAA2750.12039278693979048No Hit
GACTCAGGGGTAAACAAAGACCCAAACATTTCCCCAGGTTTTCAACTTTCCT2740.11995499498728215No Hit
TCTGTGGGCTGCATCCGGGTGTCTGGAGGCTGTGGCCGTTTTGTTTTCTTGG2710.11864161912975715No Hit
AGTTAAGGGCAATGGACTTTGAAAAAATTCTGATGCTGGACCCCACCCTTCA2710.11864161912975715No Hit
GGCCAGGGCAGAGTTAAGATAATGATTATAATACTTTAGTCCTCTTCTTGGG2690.1177660352247405No Hit
AAATATGGGGGATACTCAGTTGTGGAGAGCTGATTCCCAAATCTAAAGTTAA2680.11732824327223217No Hit
TATACAGGGGAATCAGGCCCAGGATGTCTTTTCTTAAGTAATTTCTTAATTG2670.11689045131972384No Hit
AAACAAGGGATGATAGGCCACACTATATTATCTAATTTTGATATAATTAAGC2660.11645265936721552No Hit
TCTCTTGGGCGGTGGCTACATGTTTCCAAAGCCTTTTTACACCTTGTGTTTA2660.11645265936721552No Hit
AGCACGGGCTGGGTGAGGGTACTTGTGCAAGTTCAAGCACCTTCTCACCTAT2640.11557707546219886No Hit
TATTACGGGCATGTCTATGGCTGCCTGTCCTGAGGCTCCGGAGCCCTCATTT2630.11513928350969052No Hit
AAAATGGGAGAGAAAAAGAAAAAGGAAGAAAGAATTTTGGAACTTCACGAAT2630.11513928350969052No Hit
TCTCATGGGGGAAAAAGAAAAGAAAGAGAAAAGAAAGGAAAGGAAGGAAAGA2620.11470149155718219No Hit
GCAGCATGGGACTGGATAGCATATTAAACACCTTGGTGAATTATGGTGCTTG2610.11426369960467386No Hit
CGCACTGGGGGCTTCAACTTCTAACATTATACAACAGCCTGACAATTAATCA2600.11382590765216553No Hit
TGTTCAGGGCATAAATGAATGAAATGATTCTGTCATTCTTACTTGCCCTGTA2600.11382590765216553No Hit
CTGCTTGGGGAGAGAGGGAACGAGACAGGGAAAAAGAACGGGAACGAGAAAG2600.11382590765216553No Hit
ATTGCTGGGTCGGGGTGGCTTTCTTCTGATTGAATTCCTTTTTTCTACTGGG2590.1133881156996572No Hit
CTACTAGGGTAGGAAGGCAGAGAGCTGGGCGGCAGTGTAGGTTCTGAAATTT2590.1133881156996572No Hit
TAACCAGGGAACTTAGGTGTAATTTACACATTTTTCAGATACATAAGCTATC2590.1133881156996572No Hit
GATTTGGGGGTTGTAGGTTGCCTGTTCACTCTGATGGTAGTTTCTTTTGCTG2590.1133881156996572No Hit
GGTCAAGGGTAAGTTCTGTCTTGTCAAAGCAAGAACTGTAGGTCCTGTAACT2570.11251253179464056No Hit
CATTATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2560.11207473984213223No Hit
TGCCCCGGGAGATCTCAGTCATATGAATTACAACGTAGTATTTACTGGCAAG2550.11163694788962389No Hit
TTGCTCGGGGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAA2540.11119915593711556No Hit
CGCAAAGGGATTTAAATATGATGATACATGTGAAAATATCTTACAACGTATT2530.11076136398460723No Hit
ATTCGAGGGTGTCGCAGGGCCGTGCGTGAGCTGCTGGGCCTTGGCGATCCGT2520.1103235720320989No Hit
TTACGGTGGGACTGTAGAATTAACGTTATGAAATGTCAGATGCTTGGTGAAT2500.10944798812708226No Hit
TTCCTTGGGAGTTTGATGATGATGAGAAACAGAAGCTGATCGAGGAGGTGCA2490.10901019617457393No Hit
GTGTCAGGGGCTGGGGCTTACTGGGGGCTGAAGGTTCTGGACATGAACAAGG2490.10901019617457393No Hit
GCTCAAGGGAGATGATATTGACATGCCTGGACAGAATTTTGACTCCAAGAGA2480.1085724042220656No Hit
CGTTATGGGGGCCAGCAATTAGAATTTTAACACTGGCAGTTATGAATAATAT2470.10813461226955726No Hit
CATTTAGGGTCCGATCTTCGGCGGGGAGCAAGGGGATGGTAGATATAATTGC2460.10769682031704893No Hit
TCGATAGGGCGATCTTAACACGGGGACAAGTCTGCCTTTAACAAGCTGGCCA2460.10769682031704893No Hit
TGCAAGGGCGATCTAACTCGGGGACCTATGTTCAATGACAAAGACACTCCCT2450.1072590283645406No Hit
TAGCGCTGGGAAAATTGTTAGAAAGAATATAAACTGCCAAACAAGCATGTTA2440.10682123641203227No Hit
GCTCAAGGGCAATTTATATTCAATTCATATGACTTCCCATTTCTCCACACCC2440.10682123641203227No Hit
GCTTGGGACAAATTGAAAGCTTCCATGTTTAATGTTATCCTCTTGCTATATA2440.10682123641203227No Hit
GATGTTGGGAGGAGAAAATGCTGAGAAGCTTCTGCAGTTCAAGCGTTGGTTC2430.10638344445952394No Hit
GAGCCAGGGAAATGCTCCCAGGAGTGAAGTGGAAGCCTTAAGGATGATGTCC2420.10594565250701563No Hit
TAGCCTGGGCGACAGAGTGAGACTCTGTCTCAAAAACTGAAGTACTGATTCT2410.1055078605545073No Hit
TTAATAGGGACCTGGTTAGACATGCCAGCCTTTGCAAGGCAGGTTAGTCACC2410.1055078605545073No Hit
TGAAATGGGTACTACTAGCAAACTTTTATCTAGTGATTATTGTATGTCAGGC2400.10507006860199897No Hit
TGCACAGGGACTATAATCAAATTACTATAAATCAAAGAAGCCTTATTCTGTA2390.10463227664949062No Hit
TGCTAGGGGAAGGCAAGACTTACTGTAATTATTGGTAATTTTTATAAACCAA2390.10463227664949062No Hit
CACTCCGGGAACTTGTTGAAACTGTGGCTTTAACATCCATGTGACTAGTGTA2380.1041944846969823No Hit
TGTAATGGGAATTCAGGACAGCAGAAGAGAAACTAAAAATGTTGCATCTGGA2380.1041944846969823No Hit
AACTCCGGGATTTGAGTACCAAGATAAACTTACACCACATACTTGGTGGGTG2380.1041944846969823No Hit
AACTCAGGGAACAAACACATGAAAAGATGCTTAATGTTATTAGTCATTAGGG2380.1041944846969823No Hit
TGTAGCGGGCAGGAGGAGGGCAGTGTGGTGGTGGAGCGAGCCGTTCAGACCG2380.1041944846969823No Hit
TGTTTAGGGATGGCCTGCCAGAATCCAGTTTGGAGATAAAAAGGGCTTAATT2370.10375669274447397No Hit
AGAAGCGGGAAGGACAGATTGTTATATACGATGTGGGAGAGCAGATTGCTGT2360.10331890079196564No Hit
AGTGTGGGAGAGCTGGATGTGCATGCCCTGTGGACGAAGGTACAGCTCGCCT2350.10288110883945731No Hit
GCATTTGGGGAACATTGGCCGCTTAATGGTGTGCTGATCATGAAACAAAGAT2340.10244331688694899No Hit
TTATCGGGGATGAAATTTTGTTAAATAGGCAAATGTGGAATTCCTCACAGAT2340.10244331688694899No Hit
GCTAGGGGGGTTTGTCATAAATAGCTCTGATTATTTTGAGGTACGTCCCTTC2330.10200552493444066No Hit
TACTCCGGGAGGAGGCCAAGGCGAGAAGATCACTTGAGGCTGGCCTCGAACT2330.10200552493444066No Hit
CTTGTTGGGAGCTCAAAACAATCAAGTTTTGATTTTTAGTCTGTGTGTGTGT2330.10200552493444066No Hit
GGCTATGGGGAAAAAAGTTACTGTGTAAAGAAATGCATAGTAACAAACCTCA2330.10200552493444066No Hit
AATTTAGGGCAGCTACAGCTGATTTTTCCAGTCTCATCTTTATTTTTCTCTT2320.10156773298193233No Hit
ATTGGGGAGACCCAAAAGAATGAAAGGTGGCAGTGAAGATGGGGTCTGAAAG2320.10156773298193233No Hit
CAAATAGGGGAGCACCAAGAGGCCAAGAAAGCAGAGAACTCATCAAATGAGG2320.10156773298193233No Hit
TAAGGTGGGTGAGAAGGGGAAATTTTTCAATATGTAGAAGCTTCAGAATTAG2320.10156773298193233No Hit
TATTGCGGGCTCTGGAAAGCGTCCCAGAATATTAGCCAGTTTTCTGTCACAT2310.101129941029424No Hit
AGAGTGGGGGTTTTTTGAATGTAGTCCAATCTAAATTTATAAACTAAACAGT2310.101129941029424No Hit
GGCTCCGGAAGGCAGTTTTGCTTGGGAAACAAGCTCCTTCCCCGGGGTCATC2300.10069214907691566No Hit
TCATTGGGGACATCAGAGTTTCCGGTTTCCCAATGACAAGCCTGTCTACGTA2300.10069214907691566No Hit
TATGCTGGGGGGAAGTGAGCGGCGGGGTCGACTGACGGTAACGGGGCAGAGA2300.10069214907691566No Hit
ACTTGAGGGACCTCAGTTCTCAGGCATGGGCATGGATTAAACCAGTTAGTTT2290.10025435712440733No Hit
TCTGCGGGCATTCCTGGTGTCCCAAAGAATAGCAAAAAGCCAGTTTGAATAT2290.10025435712440733No Hit
AAAAAAGGGATGGTGAGCCCCTTAGAGATGAGCGCCTACTCCTTCTTGGCGA2290.10025435712440733No Hit
ATATAGGGAAGTCGTGACAGCCTTCATCAGTGCAATGTTTGCAGGGTAATTC2290.10025435712440733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)