FastQCFastQC Report
Sat 31 Dec 2022
SRR5848614.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848614.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229836
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC8830.3841869855027063No Hit
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT7840.3411127934701265TruSeq Adapter, Index 10 (95% over 21bp)
AAAGCTGGGCAGATTTGGAACTTCTAGATGTGTAAGTAAAGCCATAAATATA3480.15141231138725003No Hit
GACTCAGGGGTAAACAAAGACCCAAACATTTCCCCAGGTTTTCAACTTTCCT3020.13139804034180894No Hit
CTGCGTTCGGGATGAAAAAGATAGGATAACATTGTTAAAGGATCACTCGGTT2990.1300927617953671No Hit
AAAATGGGTAGCATCAAGGAAGTTATGATTGGCGTGGACAAATGTTTCAGCC2940.12791729755129744No Hit
GCTCAAGGGCAATTTATATTCAATTCATATGACTTCCCATTTCTCCACACCC2900.1261769261560417No Hit
TACACTGGGCTAGTAAATGCCTCCTTAACAATTAGCCCAATTCATATCAATG2880.1253067404584138No Hit
CTGCTTGGGGAGAGAGGGAACGAGACAGGGAAAAAGAACGGGAACGAGAAAG2860.12443655476078595No Hit
AGAAGCGGGAAGGACAGATTGTTATATACGATGTGGGAGAGCAGATTGCTGT2860.12443655476078595No Hit
AAAATGGGAGAGAAAAAGAAAAAGGAAGAAAGAATTTTGGAACTTCACGAAT2850.124001461911972No Hit
TCTCTTGGGCGGTGGCTACATGTTTCCAAAGCCTTTTTACACCTTGTGTTTA2850.124001461911972No Hit
TCAGTAGGGAAAGCAGAATATTAGAGCAGATTGCAGTTGGCCCACCCACCCG2820.1226961833655302No Hit
AAATATGGGGGATACTCAGTTGTGGAGAGCTGATTCCCAAATCTAAAGTTAA2810.12226109051671627No Hit
TATGCTGGGGGGAAGTGAGCGGCGGGGTCGACTGACGGTAACGGGGCAGAGA2800.12182599766790232No Hit
GATTTGGGGGTTGTAGGTTGCCTGTTCACTCTGATGGTAGTTTCTTTTGCTG2800.12182599766790232No Hit
CTACTAGGGTAGGAAGGCAGAGAGCTGGGCGGCAGTGTAGGTTCTGAAATTT2780.12095581197027445No Hit
TATTACGGGCATGTCTATGGCTGCCTGTCCTGAGGCTCCGGAGCCCTCATTT2760.12008562627264657No Hit
TTCCTTGGGAGTTTGATGATGATGAGAAACAGAAGCTGATCGAGGAGGTGCA2760.12008562627264657No Hit
CACTTAGGGGATTGTTCCTGCCCTTCCCCAACTAATCCATCCACCTTGTGAC2760.12008562627264657No Hit
CGCACTGGGGGCTTCAACTTCTAACATTATACAACAGCCTGACAATTAATCA2750.11965053342383265No Hit
GTTCAGGGGATTTGGAAGCATTAAATCCATACTTATGGGTTTAGATTTTTTT2740.1192154405750187No Hit
TATTTTGGGGATGCTGTTGTATGCTCTTTTTGTTTGATACAGAAGCTCAGAA2690.11703997633094902No Hit
TAGCGCTGGGAAAATTGTTAGAAAGAATATAAACTGCCAAACAAGCATGTTA2680.1166048834821351No Hit
GGCTCCGGAAGGCAGTTTTGCTTGGGAAACAAGCTCCTTCCCCGGGGTCATC2670.11616979063332115No Hit
TATACAGGGGAATCAGGCCCAGGATGTCTTTTCTTAAGTAATTTCTTAATTG2670.11616979063332115No Hit
CCAGCCTGGGTGACAGAGCGAGAGTCAGTCTCAAAAAGAATAAAATAATGTT2670.11616979063332115No Hit
ATTCGAGGGTGTCGCAGGGCCGTGCGTGAGCTGCTGGGCCTTGGCGATCCGT2650.11529960493569327No Hit
TCTGCGGGCATTCCTGGTGTCCCAAAGAATAGCAAAAAGCCAGTTTGAATAT2640.11486451208687935No Hit
AAACAAGGGATGATAGGCCACACTATATTATCTAATTTTGATATAATTAAGC2620.11399432638925147No Hit
GATCAAGGGGAGAATGCAGCAAAAGCCAACGAGGCTGCTGCTAACTCCTTGC2610.11355923354043752No Hit
TGTACAGGGTCTGTAATTTTCACTTTTAACATGTTACCTAAAGCACTTTTTG2610.11355923354043752No Hit
TTTACTGGGGATGAAGGGATAAAGAAAATGTGGTATATATATACAATGGTAA2610.11355923354043752No Hit
GCTCAAGGGAGATGATATTGACATGCCTGGACAGAATTTTGACTCCAAGAGA2610.11355923354043752No Hit
CGATCTGGGATTTTTACAGCACTTTGAGACATGTCTCATTATTCCTACTTGA2600.1131241406916236No Hit
GCTTACGGGCAGCTCAGGTCAGCGCCTCCCTCCCTCGGGGAGCCCAGCGAGG2590.11268904784280966No Hit
TTACGGTGGGACTGTAGAATTAACGTTATGAAATGTCAGATGCTTGGTGAAT2560.11138376929636785No Hit
TCATTGGGGACATCAGAGTTTCCGGTTTCCCAATGACAAGCCTGTCTACGTA2560.11138376929636785No Hit
AGTTAAGGGCAATGGACTTTGAAAAAATTCTGATGCTGGACCCCACCCTTCA2560.11138376929636785No Hit
AGTGTGGGAGAGCTGGATGTGCATGCCCTGTGGACGAAGGTACAGCTCGCCT2550.1109486764475539No Hit
TGGCCTGGGAAATCTAGAGCTCCAAGGATGCTGGTCATTAAGAAAGGTAATA2540.11051358359873997No Hit
TTATCCGGGGTGCTTGTAGGATGAAATTGATAAACAATTATTTTACCAACTT2540.11051358359873997No Hit
TATCCTGGGTGCTTTGCTAAAATTAAGTATACAATATGCGTAGCAATGCCTT2540.11051358359873997No Hit
CTTCAAGGGGTTGGGATTACAGATGTGAGCTGCTGCGCCCGACCTGAAAACG2530.11007849074992603No Hit
CATATAGGGACGACCGGGTGCAGGAGAAGACAGTTGTGTTTCAACAGGAGAG2530.11007849074992603No Hit
TTGCTCGGGGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAA2520.1096433979011121No Hit
GAGCCAGGGAAATGCTCCCAGGAGTGAAGTGGAAGCCTTAAGGATGATGTCC2500.10877321220348422No Hit
GATAGTGGGCAAATGTCCTCATTTTTAACAAGGAAAGCTTATGACACACACA2490.10833811935467028No Hit
CACAGGGGGTTACTGTAAATGGAATTGCTTTAATAATTTCCTTTTCAGATTG2480.10790302650585636No Hit
CATTATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2470.10746793365704241No Hit
TGTTTAGGGATGGCCTGCCAGAATCCAGTTTGGAGATAAAAAGGGCTTAATT2470.10746793365704241No Hit
TATACAGGGTACAGCAGAGAGCCAGGAGGTCCAACTGCCGTAGACAGGAAGG2470.10746793365704241No Hit
TGCCAAGGGGATTCCAAACATGGCGGCTCCACTAGGGGGTATGTTTTCTGGG2470.10746793365704241No Hit
CATTTAGGGTCCGATCTTCGGCGGGGAGCAAGGGGATGGTAGATATAATTGC2470.10746793365704241No Hit
ATAAACGGGAAGAAACTCACCGTGATAAGCTAGCTTCCACGTCTGGAGAAAA2470.10746793365704241No Hit
TGCTAGGGGAAGGCAAGACTTACTGTAATTATTGGTAATTTTTATAAACCAA2470.10746793365704241No Hit
GGCCAGGGCAGAGTTAAGATAATGATTATAATACTTTAGTCCTCTTCTTGGG2470.10746793365704241No Hit
GTGTCAGGGGCTGGGGCTTACTGGGGGCTGAAGGTTCTGGACATGAACAAGG2460.10703284080822847No Hit
TCTCATGGGGGAAAAAGAAAAGAAAGAGAAAAGAAAGGAAAGGAAGGAAAGA2460.10703284080822847No Hit
TGTAATGGGAATTCAGGACAGCAGAAGAGAAACTAAAAATGTTGCATCTGGA2450.10659774795941453No Hit
GAAAATGGGGATTTGGGTAAAGCTTTTGTTTAAGAACCCATAGCTGCATGTT2450.10659774795941453No Hit
AATTTAGGGCAGCTACAGCTGATTTTTCCAGTCTCATCTTTATTTTTCTCTT2440.1061626551106006No Hit
TGTTCAGGGCATAAATGAATGAAATGATTCTGTCATTCTTACTTGCCCTGTA2430.10572756226178666No Hit
GCTAATGGGAGCCCAGAGAGCTTAGTCAAAGACGATATCACGGTCTACCTTA2420.10529246941297273No Hit
CTTGTTGGGAGCTCAAAACAATCAAGTTTTGATTTTTAGTCTGTGTGTGTGT2420.10529246941297273No Hit
ACTAGGGACACTCTGCAGTGCTAACATTGAGAACACATTTCTTGTTCTTTTT2420.10529246941297273No Hit
GCTTGGGACAAATTGAAAGCTTCCATGTTTAATGTTATCCTCTTGCTATATA2420.10529246941297273No Hit
GTGAATGGGAGAGCTCACAAGCTACAGAAAGAGATAAGCTTGTAAATCACAG2400.10442228371534486No Hit
AAGCCTGGGATTATGCCCTCATCTCTACCTCTATAGATTTAGCCTTTGTGAG2400.10442228371534486No Hit
CAAATAGGGGAGCACCAAGAGGCCAAGAAAGCAGAGAACTCATCAAATGAGG2390.10398719086653091No Hit
TGAGCAGGGGGGGAATTCATGTGGAGGTCAGAGTGGAAGCAGGTGTGAGAGG2390.10398719086653091No Hit
GCAGCATGGGACTGGATAGCATATTAAACACCTTGGTGAATTATGGTGCTTG2390.10398719086653091No Hit
TGAGACGGTCTCCTTCCATCACTACAGTAAGTAAAAGGACCATAGAGGGGAA2380.10355209801771698No Hit
TTAATAGGGACCTGGTTAGACATGCCAGCCTTTGCAAGGCAGGTTAGTCACC2380.10355209801771698No Hit
GCTAACGGATGCTTTTGCATATGTACATAAAAATATCTACCTAAAAATTCCT2380.10355209801771698No Hit
TCGATAGGGCGATCTTAACACGGGGACAAGTCTGCCTTTAACAAGCTGGCCA2370.10311700516890306No Hit
CGCAAAGGGATTTAAATATGATGATACATGTGAAAATATCTTACAACGTATT2370.10311700516890306No Hit
TTTATGGGGAAGTGTAGTATCACAAAGATAGGATAAGGGGGAAAAGCCACTA2350.10224681947127517No Hit
AGCACGGGCTGGGTGAGGGTACTTGTGCAAGTTCAAGCACCTTCTCACCTAT2350.10224681947127517No Hit
TAGCCTGGGCGACAGAGTGAGACTCTGTCTCAAAAACTGAAGTACTGATTCT2350.10224681947127517No Hit
AACTCAGGGAACAAACACATGAAAAGATGCTTAATGTTATTAGTCATTAGGG2350.10224681947127517No Hit
AATACGGGGGTATTTTGTGACCTTATGATTAGTTTTATCTTTCAACTGATTT2340.10181172662246123No Hit
TTACCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2340.10181172662246123No Hit
GGCTATGGGACAATTATGACAACTGAAATGAGACATGCACATAATATAGATA2340.10181172662246123No Hit
TGCTCGCGGGCGGATTACTTTGTTTTGTGGTATCCAGAGTTTGAAAGGATTG2340.10181172662246123No Hit
TCAACAGGGAGTTTAAAATTGCTTTCTAAATAGAAATGAAATACGTGATATG2330.1013766337736473No Hit
TGCAAGGGCGATCTAACTCGGGGACCTATGTTCAATGACAAAGACACTCCCT2330.1013766337736473No Hit
AACAGCGGGACTGTAATTTGAATACAATTACTGTATCTAAAAGGAGCTGCTA2330.1013766337736473No Hit
GTTAGGGGTGTTCTGTGAGGGCCTGCAGGGAACAGTGGTCCCATGGAATTAT2320.10094154092483336No Hit
CTGATAGGGGTGAATTTATTTGTTCTGTGATGTTTCTTTTTATGTTCTGCAC2320.10094154092483336No Hit
CGTGAAGGGGTACTACTAATTAATAGAAAATCATTCAACCAAGAGAAGAGTC2310.10050644807601943No Hit
CTGGCCGGGATGCCCGGAATACTACTCAGCGATAAAAAAGAACTAATTACTG2310.10050644807601943No Hit
ATATAAGGGGAGGACAGGTGTGTTGGGCGTGTTGAGAGCCTTGAAGTGACTA2300.10007135522720548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)