FastQCFastQC Report
Sat 31 Dec 2022
SRR5848613.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848613.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227705
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC7900.34694011989196544No Hit
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT7880.34606179047451746TruSeq Adapter, Index 10 (95% over 21bp)
AAAGCTGGGCAGATTTGGAACTTCTAGATGTGTAAGTAAAGCCATAAATATA3600.1580992951406425No Hit
TCAGTAGGGAAAGCAGAATATTAGAGCAGATTGCAGTTGGCCCACCCACCCG2950.12955358907358203No Hit
CACTTAGGGGATTGTTCCTGCCCTTCCCCAACTAATCCATCCACCTTGTGAC2820.12384444786016995No Hit
TATTACGGGCATGTCTATGGCTGCCTGTCCTGAGGCTCCGGAGCCCTCATTT2780.12208778902527394No Hit
TATACAGGGGAATCAGGCCCAGGATGTCTTTTCTTAAGTAATTTCTTAATTG2760.12120945960782592No Hit
AATTTAGGGCAGCTACAGCTGATTTTTCCAGTCTCATCTTTATTTTTCTCTT2760.12120945960782592No Hit
AAAATGGGTAGCATCAAGGAAGTTATGATTGGCGTGGACAAATGTTTCAGCC2740.1203311301903779No Hit
TTATCCGGGGTGCTTGTAGGATGAAATTGATAAACAATTATTTTACCAACTT2740.1203311301903779No Hit
AGAAGCGGGAAGGACAGATTGTTATATACGATGTGGGAGAGCAGATTGCTGT2740.1203311301903779No Hit
GCTCAAGGGCAATTTATATTCAATTCATATGACTTCCCATTTCTCCACACCC2720.11945280077292988No Hit
TCTCTTGGGCGGTGGCTACATGTTTCCAAAGCCTTTTTACACCTTGTGTTTA2710.11901363606420588No Hit
TCTGCGGGCATTCCTGGTGTCCCAAAGAATAGCAAAAAGCCAGTTTGAATAT2700.11857447135548188No Hit
AAATATGGGGGATACTCAGTTGTGGAGAGCTGATTCCCAAATCTAAAGTTAA2690.11813530664675786No Hit
CTGCTTGGGGAGAGAGGGAACGAGACAGGGAAAAAGAACGGGAACGAGAAAG2670.11725697722930985No Hit
GATTTGGGGGTTGTAGGTTGCCTGTTCACTCTGATGGTAGTTTCTTTTGCTG2660.11681781252058585No Hit
AAAATGGGAGAGAAAAAGAAAAAGGAAGAAAGAATTTTGGAACTTCACGAAT2650.11637864781186184No Hit
TGCTCGCGGGCGGATTACTTTGTTTTGTGGTATCCAGAGTTTGAAAGGATTG2650.11637864781186184No Hit
AACAGCGGGACTGTAATTTGAATACAATTACTGTATCTAAAAGGAGCTGCTA2650.11637864781186184No Hit
TTTACTGGGGATGAAGGGATAAAGAAAATGTGGTATATATATACAATGGTAA2610.11462198897696581No Hit
CATCGTGGGTCATAGGCCAGCTTTAGTGACAAAGCTCAGAGACCAATTTCAA2590.11374365955951779No Hit
TTTATGGGGAAGTGTAGTATCACAAAGATAGGATAAGGGGGAAAAGCCACTA2580.11330449485079379No Hit
GTTCAGGGGATTTGGAAGCATTAAATCCATACTTATGGGTTTAGATTTTTTT2580.11330449485079379No Hit
CGCACTGGGGGCTTCAACTTCTAACATTATACAACAGCCTGACAATTAATCA2560.11242616543334577No Hit
GGCCAGGGCAGAGTTAAGATAATGATTATAATACTTTAGTCCTCTTCTTGGG2560.11242616543334577No Hit
CACAGGGGGTTACTGTAAATGGAATTGCTTTAATAATTTCCTTTTCAGATTG2540.11154783601589775No Hit
GCTTGGGACAAATTGAAAGCTTCCATGTTTAATGTTATCCTCTTGCTATATA2540.11154783601589775No Hit
TCTGTGGGCTGCATCCGGGTGTCTGGAGGCTGTGGCCGTTTTGTTTTCTTGG2530.11110867130717375No Hit
CGCAAAGGGATTTAAATATGATGATACATGTGAAAATATCTTACAACGTATT2530.11110867130717375No Hit
TTCCTTGGGAGTTTGATGATGATGAGAAACAGAAGCTGATCGAGGAGGTGCA2520.11066950659844976No Hit
ATATGAGGGACTGTGTACTACCTGCATTTGTGCTGTGTGAAAAATAAATACA2520.11066950659844976No Hit
CGCACAGGGATTCTCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTG2510.11023034188972573No Hit
GTGTCAGGGGCTGGGGCTTACTGGGGGCTGAAGGTTCTGGACATGAACAAGG2500.10979117718100175No Hit
TGTACAGGGTCTGTAATTTTCACTTTTAACATGTTACCTAAAGCACTTTTTG2490.10935201247227772No Hit
ATTCGAGGGTGTCGCAGGGCCGTGCGTGAGCTGCTGGGCCTTGGCGATCCGT2490.10935201247227772No Hit
CTACTAGGGTAGGAAGGCAGAGAGCTGGGCGGCAGTGTAGGTTCTGAAATTT2490.10935201247227772No Hit
TACCATGGGCCTCAAATTTGATTGTATGGTTAACAAACTGCAGACAGCTCAT2490.10935201247227772No Hit
TACACTGGGCTAGTAAATGCCTCCTTAACAATTAGCCCAATTCATATCAATG2490.10935201247227772No Hit
AGTTAAGGGCAATGGACTTTGAAAAAATTCTGATGCTGGACCCCACCCTTCA2490.10935201247227772No Hit
GCTCAAGGGAGATGATATTGACATGCCTGGACAGAATTTTGACTCCAAGAGA2490.10935201247227772No Hit
AGCACGGGCTGGGTGAGGGTACTTGTGCAAGTTCAAGCACCTTCTCACCTAT2480.10891284776355373No Hit
AACTCCGGGATTTGAGTACCAAGATAAACTTACACCACATACTTGGTGGGTG2480.10891284776355373No Hit
GTGTGAGGGGGAAAAAGAGGGAAGGAAGCAAGGAAGGGACCATCTTTCTGCC2470.10847368305482973No Hit
CTTCAAGGGGTTGGGATTACAGATGTGAGCTGCTGCGCCCGACCTGAAAACG2470.10847368305482973No Hit
GGCTATGGGGAAAAAAGTTACTGTGTAAAGAAATGCATAGTAACAAACCTCA2460.1080345183461057No Hit
CATATAGGGACGACCGGGTGCAGGAGAAGACAGTTGTGTTTCAACAGGAGAG2450.10759535363738171No Hit
GAAAATGGGGATTTGGGTAAAGCTTTTGTTTAAGAACCCATAGCTGCATGTT2450.10759535363738171No Hit
ATTGCTGGGTCGGGGTGGCTTTCTTCTGATTGAATTCCTTTTTTCTACTGGG2440.10715618892865768No Hit
TATTTTGGGGATGCTGTTGTATGCTCTTTTTGTTTGATACAGAAGCTCAGAA2440.10715618892865768No Hit
GACTCAGGGGTAAACAAAGACCCAAACATTTCCCCAGGTTTTCAACTTTCCT2430.10671702421993369No Hit
CTTGTTGGGAGCTCAAAACAATCAAGTTTTGATTTTTAGTCTGTGTGTGTGT2430.10671702421993369No Hit
TTGCTCGGGGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAA2430.10671702421993369No Hit
GCAGCATGGGACTGGATAGCATATTAAACACCTTGGTGAATTATGGTGCTTG2430.10671702421993369No Hit
GGTCAAGGGTAAGTTCTGTCTTGTCAAAGCAAGAACTGTAGGTCCTGTAACT2410.10583869480248567No Hit
TAAATAGGTACTACTGATTTATGAGGCAATGACAATGCATATTTCCTTATTC2390.10496036538503765No Hit
CTGCGTTCGGGATGAAAAAGATAGGATAACATTGTTAAAGGATCACTCGGTT2380.10452120067631365No Hit
TCGATAGGGCGATCTTAACACGGGGACAAGTCTGCCTTTAACAAGCTGGCCA2380.10452120067631365No Hit
CACGAAGGGAAACGAGGATTCCTATAAGGACTCCACGCTGATCATGCAGTTG2380.10452120067631365No Hit
GTGGGTCGGGACTTCTGGAAGCTTAACAAAACTAACCCTGCTGTCCTTTTTA2370.10408203596758965No Hit
AAACAAGGGATGATAGGCCACACTATATTATCTAATTTTGATATAATTAAGC2370.10408203596758965No Hit
TAACTAGGGACACTTGAAGTATACAGTCTTTCACAGCTCTATATTAATCACT2370.10408203596758965No Hit
TGCAAGGGCGATCTAACTCGGGGACCTATGTTCAATGACAAAGACACTCCCT2370.10408203596758965No Hit
GACAAGGGGCATCTGTCTGATGCAGACAGATGTGGATTTGGATCCTGGCTCT2370.10408203596758965No Hit
TGTTTAGGGATGGCCTGCCAGAATCCAGTTTGGAGATAAAAAGGGCTTAATT2360.10364287125886563No Hit
TCAATGGGCTACTGCTGATGATACCAGTGCTGCTGTAACTGAAGAAATGCCA2360.10364287125886563No Hit
AACAAGGGGCACCTTCTCCACAGTTGTTGACAGACAGCAAATAAAGGCAACA2360.10364287125886563No Hit
TCATCTGGCATTCTTTTTTCTCTTTCATTTTTGAGTAGTTTCTTTTGCTATA2350.10320370655014163No Hit
TTGGAGGGGAAGTATTATACAATTTAAAAACTCTTGTTCCAAAGTCCTGTCT2350.10320370655014163No Hit
GAGCCAGGGAAATGCTCCCAGGAGTGAAGTGGAAGCCTTAAGGATGATGTCC2340.10276454184141763No Hit
TAACCAGGGAACTTAGGTGTAATTTACACATTTTTCAGATACATAAGCTATC2340.10276454184141763No Hit
TGCACAGGGACTATAATCAAATTACTATAAATCAAAGAAGCCTTATTCTGTA2330.10232537713269362No Hit
TCGGCTTGGGGGGAAACGGAAGTGAGCGGCGGGGTCGACTGACGGTAACGGG2330.10232537713269362No Hit
GCACCCGGGAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGC2330.10232537713269362No Hit
AACGCAGGGCGTGGGCGTTTGAAGTGAAGTGATGTTGCAAGGCGACTTGCAA2330.10232537713269362No Hit
TGTTTTGGGGACTCTCGCGAGGCCCCAGAGAGCAGGCGCTGGGCAGTGTGGA2320.10188621242396961No Hit
CATTTAGGGTCCGATCTTCGGCGGGGAGCAAGGGGATGGTAGATATAATTGC2320.10188621242396961No Hit
GCATTTGGGGAACATTGGCCGCTTAATGGTGTGCTGATCATGAAACAAAGAT2320.10188621242396961No Hit
CGTGGGGGCACCCGAAGCGCACGGCCACCCTCAGCATGAGGAACACGAGCGT2320.10188621242396961No Hit
GAAAGTGGGCTTTTGAATAGAACAAACAGCTTTTCCATTTTGCTCTGCTGCA2320.10188621242396961No Hit
CATTATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2310.1014470477152456No Hit
TGCTAGGGGAAGGCAAGACTTACTGTAATTATTGGTAATTTTTATAAACCAA2310.1014470477152456No Hit
CGCGTTGGGGATCATATTTCTAAACAAAAGGAAGAAATTCGCAGATGCATAC2310.1014470477152456No Hit
ATAACGGGGGTCACAGAAAATAGATTTCTTGCTTGCAAAATCTACATCGCCC2300.1010078830065216No Hit
AGTCAGGGTGAATGAAAGCGCTAAAATGTCTCAATCCAGACGTACCCACTGA2300.1010078830065216No Hit
GGCATGGGATAGAAATGTTGAACTGGATGAGTCGCTTGTTCTCTTTTGAACT2300.1010078830065216No Hit
CCACGGGAGGCTAAGCAAAGAAAAGAAAAAAGACAAATGACCAATATCAGGA2290.1005687182977976No Hit
GGCTCCGGAAGGCAGTTTTGCTTGGGAAACAAGCTCCTTCCCCGGGGTCATC2290.1005687182977976No Hit
AATCACGGGGTTAATAAATTCCCACTAGAGTGACACTGAAGATTTAAACACA2290.1005687182977976No Hit
TATCCTGGGTGCTTTGCTAAAATTAAGTATACAATATGCGTAGCAATGCCTT2290.1005687182977976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)