FastQCFastQC Report
Sat 31 Dec 2022
SRR5848600.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848600.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182125
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC8400.4612216884008236No Hit
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT8260.4535346602608099TruSeq Adapter, Index 10 (95% over 21bp)
TATAGCGGGATTCAGACTTCGAAAAAATAGTTTGTTGTTGCTTGTTAAATTT2470.13562113932738504No Hit
CAAGCTGGGAAGCGGAGGGAGTCGAGCCAGCGTCGCCGCGATGGTGTTGTTG2330.1279341111873713No Hit
GCAGCAGGGGGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTC2260.12409059711736443No Hit
CACAAGAGGAAAAAAAGCAGCAAGACAGAGATTTGTTGGTGCCAACTAAATG2250.12354152367879204No Hit
GCTTGAGGGGATTTGCAGTAATTTGCAATTTGTCAGTGTATAAGACACATGC2240.12299245024021963No Hit
GTCTAAGGGGCTCACTGCAACATCCACCTCCTGGGTTCGAGCAATTCTCCTG2220.1218943033630748No Hit
AAAGCAGGGTGTGGAGGAAGGGAAATATCCGTCATCCAAAGATTCTATATCT2210.1213452299245024No Hit
CGCTATGGGTAGTAAGTGAGTTCTCACGAGATCTGATGGTTTTATGGGGGGC2210.1213452299245024No Hit
TTTAATGGGACCCCTCCACTCTATTTTTGTGTTGTTTTGCACAGACTCGGGA2180.11969800960878517No Hit
GATTCCGGGGTAGGCACTGTGATCATGATGAAAATGAATGAGGATGGCCTGA2170.11914893617021277No Hit
GGTCTAGGGGTTTTCACAGATAATTTTCTAGCGTATAGGCTATTTGCCTTTT2160.11859986273164036No Hit
GTATGGGGGTGGTCTGCTGCACCTATGAACCGGTCATCTAGGTTTTAAGCCC2110.1158544955387783No Hit
GCACTGCAGACGGGAATGCCTAGGTCCCTTCCCGACCCAGCCAGCTGCAGGG2110.1158544955387783No Hit
TCACCTGGGTCATAAGGATTAAAAAAATGCATCTTTAAATTCCACTTGAGGT2110.1158544955387783No Hit
AGCTTGGGGAGACCCTCCAAATCCCTGTCCTGTTGGAAAAACAGATGATGGA2100.1153054221002059No Hit
CGCGTAGGGAGCATGGGGGCCCAGTGCTTTTACAACAGAGGAATGCTAAGGG2090.1147563486616335No Hit
ATATCGGGGATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTA2090.1147563486616335No Hit
CTTGCAGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA2090.1147563486616335No Hit
ACGCCAGGGGACGACTTCGGCTTTACTGTAAAGGGGCTGTAAGTACCGGAGA2090.1147563486616335No Hit
TTGCTTGGGAGCATCAGCAGAACAGAAAATGTGATGTATTTTATGCATGTCA2090.1147563486616335No Hit
GTGCATGGGACCAGCGGCTAAGTCTTTTTTCTAGTTCGCAATGTTTTGTTTT2080.11420727522306108No Hit
ACGTAAGGGTCTGTGTGAGGGAGTAACACTCCTGATACAACTCTGGTTATAA2080.11420727522306108No Hit
TATGAGGGGGAGAGAGATCCTTCTTTTAAAGTGATTTTATCAAAGCCCCTTG2070.11365820178448867No Hit
AAAGCTGGGAAGGAAGGTGAAATAAAACTATTTTCAGGAAATAAAAATTAAG2070.11365820178448867No Hit
TTCGTTGGGCAGTAGGAGGACTAGGCTCCATTATAAGAGTTCTTACAGCAAG2070.11365820178448867No Hit
CACTCCGGGATTCAAACCTTTTGCATGTTAAAAAAAATTTTCTTACTGATTT2050.11256005490734386No Hit
AAATGGTGGGGTTTGGGAGATAGGATGAAGTCATGATTTTGCTTTTGCAAGT2030.11146190803019904No Hit
CTTGCGCCGGGAAGTCACAAAAGTAAGCTGTGACATTGTCTATAAGCATTTG2030.11146190803019904No Hit
GACTCAGGGCTGACTTTGGTCAGACTTTTGCAAATTATGGAGAGAGGCCATC2030.11146190803019904No Hit
CAATATGGGGATCTACAGCGGGGAATGTTAATAGTGATATGTGCCTTTAAAG2020.11091283459162664No Hit
GGAGATGGGTCTTCCGATCTTTGTGAGGGTTGCCCTGGAAATGTTTGCTTAC2020.11091283459162664No Hit
GACCTAGGGATCGATCAGATGTTTATTTGGTCTGAAAAATGGATTCAATACT2010.11036376115305421No Hit
TGCATCGGGAAGCCTGAGGCTGCAGCCTTTTATTTATGTTTTCCCTTTGGCT2010.11036376115305421No Hit
CACAGGGACTCCTAAAACTGCTATACTGTGAACATATTGCTTGTCTCATAAC1990.1092656142759094No Hit
CAACTTGGGTTGTCAGTAATGTACTGTGTGATTTTATAAAAATAGCATAGAC1990.1092656142759094No Hit
TTTAGCGGGGCTTTTAGAGTTGTTGAGCTTTTATTACTACTACTCATTGGGG1990.1092656142759094No Hit
TCTTTTGGGAGGGGGCACAATGGCAAACAGGATATCCCACACTTCTTAACAT1980.108716540837337No Hit
TCAGTGGGCAATTGGCAAGGGATTGATTCTTCAATGGGTTCAGCAATTCACT1970.10816746739876458No Hit
AACAGGGAGGAGCAGTGTGACTTCTTGCCACAAGGCAGAACCAGGGCAACTA1970.10816746739876458No Hit
ACAAATGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGAGAGACGCAGCGG1970.10816746739876458No Hit
GTTTTTGGGAAAGACTAAAGATTATGGAATATTATGAGAAGAAAGAGAAACA1970.10816746739876458No Hit
GGATACGGGATCGTGGCAGAGTTGACTGGAGACAACATTCCTAAGGACTTTA1970.10816746739876458No Hit
GCATAAGGGGTGGCAAAGCAGATATAAAACAGGCATGGACGCATGATTTTAG1970.10816746739876458No Hit
TTTGCTGGGCTCTTAATGCCTGGCTTAAATGAAAACTGCTGGAGTCCCATAT1970.10816746739876458No Hit
CGTGACGGGGTATGACTGAGGTATCCTGCGGTGGCTGGCGTGCTGGGCATGG1960.10761839396019217No Hit
CAAGTTGGGAGAACTATACCTAATTCTATTGTAGCTATAAGTAAATTGCTTG1940.10652024708304735No Hit
TGCCTTGGGTATGCCAGTAACTTTGCTTATCTGTCCAATTAGTCTGGCAAGC1940.10652024708304735No Hit
GTAATAGGGAATTCCTTGTTGTTCATAAATTAAGCATCACTAAAGTCTCTTG1930.10597117364447495No Hit
GCTCAAGGGATGTGGAGTAGGCTACATGGAATACCGTTTGAGTTTGTGTAAG1930.10597117364447495No Hit
GGTGCAGGGATTTCAGCTGAGACTACCATTTTGAAGTGAAGAGTCTAAGGCA1930.10597117364447495No Hit
ACAACCGGCACGACAGCCCCGAAGATGTGAAGCGACGAGCCATGGCCGACCC1930.10597117364447495No Hit
CGCTTTGGGTACATTTGGTCTCAGAATTTAGGTTCCTGCCCTGTTGGTTTTT1920.10542210020590254No Hit
AAAGCAGGGGGATTGTTGTCCCAACCCTGTACTGTACTCCCAGTGGCCAAAC1920.10542210020590254No Hit
ACATGCTAGGGCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACA1910.10487302676733014No Hit
TGCGCAGGGAGTACTGGGATTACAGGCATGAGCCACCGCGCCCAGCCTGTGC1910.10487302676733014No Hit
CACAGGGGGAATGGTTGTAGGGACTGAAAGCTGAGTTGGAAGGATCAGATCA1910.10487302676733014No Hit
GTAGGCGGGAAGTCACCCTAGCCATCATTCTACTATCAACATTACTAATAAG1900.10432395332875771No Hit
TTACGTGGGTAGCTCCACAAGAGAGATGATACTGACTTTTTAAATTTTTTAC1900.10432395332875771No Hit
TTTAAGGGGATGTAACAATGACAAAAATTATAAAAAGACATGGCTGGGCCCC1890.10377487989018531No Hit
ATCTTAGGGGTACTACCTTAGGCAGTGACTCATATCTCTGAGCCTCAGTGTC1890.10377487989018531No Hit
CGTAGGGGCAACAGTGGCAGAGTCATTTTGCTGGTTTAGGCCCTGCCATATT1880.1032258064516129No Hit
CGCGCAGGGGGGCTCTGATTAAAAAAGGAAAACTTCTAATATATTGCTTGGA1880.1032258064516129No Hit
GAATGTGGGGTTTGATAATAATGCTCATTGTTCCTTATTTTGCTTTAAAGAA1880.1032258064516129No Hit
GCAATAGGGTTGATCCTAAAGTGGAGGATACTGGAAACATCTCTGGACCTAC1880.1032258064516129No Hit
ATGCCTGGGCAGTATATCCTCTTTATGATCAGATTCCTGGATCATTGTCATT1870.1026767330130405No Hit
GGGATGGGAACACTGAGGAACCAGAAATCTGGAGGGACATCTGAGATCACCT1870.1026767330130405No Hit
GCATCTGGGATGGTAGCTCTATTTAGTAACTGTCCTGTTGCTTACCAAATCC1870.1026767330130405No Hit
AAAAATGGGTGTCTCAGAAATCATGGCCAGTGTTTAAGAAATAAGTGTGATT1870.1026767330130405No Hit
TAAACGGGGACTCCTGGCTTCAGGCGGTTCTCCCGCTTTGGCCTCCCAAAGT1860.10212765957446808No Hit
TGAAAAGGGTTATCGCAGATATTATCTCTCTATACAAACGAGTAAACTGACA1850.10157858613589568No Hit
TCCTCTGGGCGATTAATACATCATTAACTAAGGTTTGTATTTTATTTTGATT1850.10157858613589568No Hit
TGAACTCTTGACCTCAAGTGATCCGCCCACCTCGGCCTCCCGAAGTGCTGGG1840.10102951269732327No Hit
GCTTTTGGGAATAATGTTCCTTTCTCTACCAGATATTAAAACATATTAACTT1830.10048043925875084No Hit
CTACAACGGGGATTCAGAATTTGATGAAGTAGATTGGTTGGAGTAAGAAAAA1830.10048043925875084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)