FastQCFastQC Report
Sat 31 Dec 2022
SRR5848599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182640
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGC8210.4495181778361805No Hit
CTTATACACATCTCCGAGCCCACGAGACATGTGCTTATCTCGTATGCCGTCT7720.42268944371441086TruSeq Adapter, Index 10 (95% over 21bp)
TATAGCGGGATTCAGACTTCGAAAAAATAGTTTGTTGTTGCTTGTTAAATTT2650.1450941743320193No Hit
TCACCTGGGTCATAAGGATTAAAAAAATGCATCTTTAAATTCCACTTGAGGT2420.13250109505037233No Hit
CACAAGAGGAAAAAAAGCAGCAAGACAGAGATTTGTTGGTGCCAACTAAATG2400.1314060446780552No Hit
GCAGCAGGGGGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTC2370.1297634691195795No Hit
AAAGCAGGGTGTGGAGGAAGGGAAATATCCGTCATCCAAAGATTCTATATCT2360.12921594393342095No Hit
GTCGGGGATAATCACTTGTTCCTTAAGTAGGGACCCGTATGAATGGCTCCAC2310.12647831800262813No Hit
CTTGCAGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA2230.12209811651335961No Hit
GCACTGCAGACGGGAATGCCTAGGTCCCTTCCCGACCCAGCCAGCTGCAGGG2220.12155059132720106No Hit
TCTTTTGGGAGGGGGCACAATGGCAAACAGGATATCCCACACTTCTTAACAT2200.12045554095488392No Hit
GTAATAGGGAATTCCTTGTTGTTCATAAATTAAGCATCACTAAAGTCTCTTG2190.11990801576872535No Hit
GGTCTAGGGGTTTTCACAGATAATTTTCTAGCGTATAGGCTATTTGCCTTTT2180.1193604905825668No Hit
GCAATAGGGTTGATCCTAAAGTGGAGGATACTGGAAACATCTCTGGACCTAC2160.11826544021024966No Hit
GTCTAAGGGGCTCACTGCAACATCCACCTCCTGGGTTCGAGCAATTCTCCTG2150.11771791502409111No Hit
CGCGCAGGGGGGCTCTGATTAAAAAAGGAAAACTTCTAATATATTGCTTGGA2130.11662286465177397No Hit
GATTCCGGGGTAGGCACTGTGATCATGATGAAAATGAATGAGGATGGCCTGA2120.11607533946561542No Hit
GCTCAAGGGATGTGGAGTAGGCTACATGGAATACCGTTTGAGTTTGTGTAAG2110.11552781427945685No Hit
AACAATGGGGTGAGAGTGACATTCTTGTCTTGTTCCAGTTCTCAAGGGGAAT2100.11498028909329829No Hit
TTCGTTGGGCAGTAGGAGGACTAGGCTCCATTATAAGAGTTCTTACAGCAAG2070.1133377135348226No Hit
ACATGCTAGGGCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACA2040.1116951379763469No Hit
GAGTATGGGATGGATCGGGCTGTGGCTGAGGGAAAGCGTGTGTGTGTGATTG2040.1116951379763469No Hit
TTTAGCGGGGCTTTTAGAGTTGTTGAGCTTTTATTACTACTACTCATTGGGG2040.1116951379763469No Hit
CACAGGGACTCCTAAAACTGCTATACTGTGAACATATTGCTTGTCTCATAAC2030.11114761279018834No Hit
ACGCCAGGGGACGACTTCGGCTTTACTGTAAAGGGGCTGTAAGTACCGGAGA2030.11114761279018834No Hit
AAAGCTGGGAAGGAAGGTGAAATAAAACTATTTTCAGGAAATAAAAATTAAG2030.11114761279018834No Hit
AGCTTGGGGAGACCCTCCAAATCCCTGTCCTGTTGGAAAAACAGATGATGGA2020.11060008760402978No Hit
TACAGGGATTATTAGGAAGTAAAGACCAAATCTACAACTTTGATCTGGAAAT2010.11005256241787122No Hit
GAAAAGGGGATATTCAAAAGTTCTGTGGTGTTATGTCCAGTGTAGCTTTTTG2010.11005256241787122No Hit
CTTGCGCCGGGAAGTCACAAAAGTAAGCTGTGACATTGTCTATAAGCATTTG2000.10950503723171265No Hit
TCAGTGGGCAATTGGCAAGGGATTGATTCTTCAATGGGTTCAGCAATTCACT1990.1089575120455541No Hit
TATGAGGGGGAGAGAGATCCTTCTTTTAAAGTGATTTTATCAAAGCCCCTTG1990.1089575120455541No Hit
CGCTATGGGTAGTAAGTGAGTTCTCACGAGATCTGATGGTTTTATGGGGGGC1990.1089575120455541No Hit
GTAAGGGGGCGATCTACGATGGGGGAAACAGGACCTCACCAAATGAACTAAA1980.10840998685939553No Hit
ATATCGGGGATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTA1980.10840998685939553No Hit
AGTGCGGGATTAAGTATGGTGCTGGAAGTTCCAACCACTGAAATAAGGTAAG1980.10840998685939553No Hit
CACCGCGGGATGTGCAGAGAGACTAAAGTCCGAACTGATCTTCATTTCAGTC1970.10786246167323696No Hit
TTGCTTGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTAT1970.10786246167323696No Hit
GTATGGGGGTGGTCTGCTGCACCTATGAACCGGTCATCTAGGTTTTAAGCCC1970.10786246167323696No Hit
AAAAATGGGCAAAAGGTAATCTTGTTTGTTTACTATTGTCTTTTTAGGTGAT1970.10786246167323696No Hit
CACAGGGGGAATGGTTGTAGGGACTGAAAGCTGAGTTGGAAGGATCAGATCA1970.10786246167323696No Hit
ACGTAAGGGTCTGTGTGAGGGAGTAACACTCCTGATACAACTCTGGTTATAA1960.1073149364870784No Hit
GCTTGAGGGGATTTGCAGTAATTTGCAATTTGTCAGTGTATAAGACACATGC1960.1073149364870784No Hit
GACCTAGGGATCGATCAGATGTTTATTTGGTCTGAAAAATGGATTCAATACT1950.10676741130091984No Hit
AAATGGTGGGGTTTGGGAGATAGGATGAAGTCATGATTTTGCTTTTGCAAGT1950.10676741130091984No Hit
TTTGCTGGGCTCTTAATGCCTGGCTTAAATGAAAACTGCTGGAGTCCCATAT1950.10676741130091984No Hit
AACAGGGAGGAGCAGTGTGACTTCTTGCCACAAGGCAGAACCAGGGCAACTA1940.10621988611476127No Hit
TAGCCTGGGGAGGTTCTCGGACCCCAGCCTGCTGGCCCTGGGGCGCGGGGCG1940.10621988611476127No Hit
CTACAACGGGGATTCAGAATTTGATGAAGTAGATTGGTTGGAGTAAGAAAAA1940.10621988611476127No Hit
TTTAAGGGGATGTAACAATGACAAAAATTATAAAAAGACATGGCTGGGCCCC1940.10621988611476127No Hit
GACTCAGGTGTTCTCAAAGGTTATAATTCATGAAACTCATGAAGGTGTTGAA1930.10567236092860272No Hit
CGGTGGGCGAAATGCTGCACTATTAAATTAGAGGTTTTTGAAAAATCCAACT1930.10567236092860272No Hit
ACGTCAGGGTAAGCCAAACTACAACCAACTAGTTTGTTAACAAGATTAGTGC1920.10512483574244415No Hit
GTTCGTGGGGAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGA1920.10512483574244415No Hit
CGCAGGGGGCCCATGATTATCTGGAAACAGCTGGTTCCGTTTCACCATCGTT1910.10457731055628558No Hit
GTCGAGGGATGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCACCT1910.10457731055628558No Hit
AATTTCGGGGATCTATGCACGGGGGAAATGCGTGTTCTAGCTTTCTGTGTGC1910.10457731055628558No Hit
CGTGACGGGGTATGACTGAGGTATCCTGCGGTGGCTGGCGTGCTGGGCATGG1910.10457731055628558No Hit
TTGCTTGGGAGCATCAGCAGAACAGAAAATGTGATGTATTTTATGCATGTCA1910.10457731055628558No Hit
TGGGCTGGGCAGTCCATTGTCTGGAACTTAAAAGAAACTCACCACTTTCCAC1910.10457731055628558No Hit
TTTACAGGGAGAACTTTGACTATTTAAAAGTTTCATTTTCTTTTGCAATTTT1900.10402978537012703No Hit
CAACATGGGGATGTAGTGAATGCTTTCCAGAGTGGAAGTTCCATTCAGGGGG1900.10402978537012703No Hit
CGCTTTGGGTACATTTGGTCTCAGAATTTAGGTTCCTGCCCTGTTGGTTTTT1900.10402978537012703No Hit
TTCTTTGGGATTTTATGAATGCACTTAATTGTATTAAAATTACTTTTAAAAA1900.10402978537012703No Hit
TAGAGACGGGATCTTATGCTGGGGGACCTTTCCGAGTTGGCTGCAGATTTGT1900.10402978537012703No Hit
GACTCAGGGCTGACTTTGGTCAGACTTTTGCAAATTATGGAGAGAGGCCATC1890.10348226018396846No Hit
ATGTTAGGGAGGGGTTGCAATCCTGGTCTCTGATAAAACAGACTTTAAACCA1880.10293473499780989No Hit
TGCGCAGGGAGTACTGGGATTACAGGCATGAGCCACCGCGCCCAGCCTGTGC1880.10293473499780989No Hit
CAATATGGGGATCTACAGCGGGGAATGTTAATAGTGATATGTGCCTTTAAAG1870.10238720981165132No Hit
TATCAGGGGACTGCTACTTTCATGTTGCTTTCTAGAAAACAGCATTTCATTC1870.10238720981165132No Hit
AAAGCAGGGGGATTGTTGTCCCAACCCTGTACTGTACTCCCAGTGGCCAAAC1870.10238720981165132No Hit
CAGTCAGGGCTAGGCTACAAGCCTGTACAGCATGTTACTGTACTGAATACTG1860.10183968462549277No Hit
ACAAATGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGAGAGACGCAGCGG1860.10183968462549277No Hit
GGAGATGGGTCTTCCGATCTTTGTGAGGGTTGCCCTGGAAATGTTTGCTTAC1860.10183968462549277No Hit
TTACTAGGGGTTGTTCAGGGAGAAACTGACACCACTCAACTGCCTGGCACTG1860.10183968462549277No Hit
AAAAATGGGTGTCTCAGAAATCATGGCCAGTGTTTAAGAAATAAGTGTGATT1860.10183968462549277No Hit
TGCATCGGGAAGCCTGAGGCTGCAGCCTTTTATTTATGTTTTCCCTTTGGCT1850.1012921594393342No Hit
AGAATGGGGCTTGTTTAATGGGGCAGATATGCATTAAATAGTTTGTACAAGC1850.1012921594393342No Hit
CGCGTAGGGAGCATGGGGGCCCAGTGCTTTTACAACAGAGGAATGCTAAGGG1850.1012921594393342No Hit
GTTTTTGGGAAAGACTAAAGATTATGGAATATTATGAGAAGAAAGAGAAACA1850.1012921594393342No Hit
TAACAAGGGAGGAAGAGCCGAGGTGCAGGAGAGGCAAGGAGCCAACATGGCG1840.10074463425317563No Hit
CAACTTGGGTTGTCAGTAATGTACTGTGTGATTTTATAAAAATAGCATAGAC1840.10074463425317563No Hit
GCTTTAGGGGGGCGTCGCCTGCGCGTCTCGGCTGAGCTGGCCATGGCGCAGC1830.10019710906701708No Hit
CACCTAGGGGCCACAAAGTGCCTCGTTTACCTTTAAATACTGTTAATGTGTC1830.10019710906701708No Hit
TAGCCATGGGATTATTGAGGCTTTCACATATCAGTGATTTTAAAATATCAGT1830.10019710906701708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)