FastQCFastQC Report
Sat 31 Dec 2022
SRR5848588.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848588.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225868
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC7180.31788478226220623No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT6540.28954964846724635Illumina PCR Primer Index 12 (95% over 21bp)
AGCCGGGGGGGCATGCTCGAAGGGTGGGGGTTGAGGCCGACGGGGCGCCGTA2930.12972178440505075No Hit
AGATGTGGGCTGTTTCAATCCTTTCCTAGAGCAACCCTGTTTTAGGGGAACA2870.12706536561177326No Hit
GATACCGGGGATCTAACGCGGGATAATCAAAATTCCTAGAAGAGGAACAACA2760.12219526449076452No Hit
CTCAGTGGGACTCTAATGTAAACTGTGGACTTTAGTTAATACTAATGTGTCA2730.12086705509412576No Hit
AGCATCGGGCAACATGTGGCTTATATAATTCCCACACATAAAGTTCCAATAG2690.11909610923194076No Hit
AGCAAAGGGAAATTAACCGGCCTTCCAACTTTTGTCTGCCTCATTCTAAAAT2680.11865337276639454No Hit
ACTCGGGAGGATAAGCTCTGCAGTACTGCAGATTAAAGAAAATGGAATGTCA2500.11068411638656206No Hit
AAGTCAGGGTACCACAGAAATGTGTTCTGAGTTTTTCTTACCAATCTAAGCC2500.11068411638656206No Hit
CATGATGGGGGTGGCGAAAGAGCCGGCGGAGCCGGAGACCCGCTCCCGGAGA2470.10935590698992331No Hit
GCCTATGGGAATTTTCAAATATATGGACATTTCATGTACATTTTCTTTTTTA2450.1084704340588308No Hit
GTTCCGGGGGATCTACGCGCGGGACTAAAATGCAGATTCTGATTCAGTAAAT2410.10669948819664583No Hit
TGTCGACGGGATGGATCTGTGATGGCACTGGGATTGCCAATGAGTGAAATAG2350.10404306940336834No Hit
TCCAACGGGTTAAACTTTGCATAAATCCTATTGTTATATTGAGAGATTAAGA2330.10315759647227583No Hit
AGGTGCGGGGTTTAAATCTTTAATCCATCTTGAATTGATTTTTGTATAAGGT2310.10227212354118335No Hit
TGGATTGGGACCCAGAGAAGCTGATGAGCTCTGCCAGCCTTGTTAAGGAGCT2280.10094391414454461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)