FastQCFastQC Report
Sat 31 Dec 2022
SRR5848587.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848587.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224894
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC6970.3099237863171094No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT6630.294805552838226Illumina PCR Primer Index 12 (95% over 21bp)
AGCATCGGGCAACATGTGGCTTATATAATTCCCACACATAAAGTTCCAATAG2850.12672636886711075No Hit
AGCCGGGGGGGCATGCTCGAAGGGTGGGGGTTGAGGCCGACGGGGCGCCGTA2780.12361379138616416No Hit
CTCAGTGGGACTCTAATGTAAACTGTGGACTTTAGTTAATACTAATGTGTCA2770.12316913746031463No Hit
GATACCGGGGATCTAACGCGGGATAATCAAAATTCCTAGAAGAGGAACAACA2700.12005655997936807No Hit
GCTTTTGGGGTTCAGGGCCCTAGTAGCAGGAGAGATGATCCTGAATCCTGTT2610.11605467464672245No Hit
AGCAAAGGGAAATTAACCGGCCTTCCAACTTTTGTCTGCCTCATTCTAAAAT2610.11605467464672245No Hit
TCCAACGGGTTAAACTTTGCATAAATCCTATTGTTATATTGAGAGATTAAGA2490.11071882753652831No Hit
CATGATGGGGGTGGCGAAAGAGCCGGCGGAGCCGGAGACCCGCTCCCGGAGA2490.11071882753652831No Hit
ACTCGGGAGGATAAGCTCTGCAGTACTGCAGATTAAAGAAAATGGAATGTCA2480.11027417361067882No Hit
TGGATTGGGACCCAGAGAAGCTGATGAGCTCTGCCAGCCTTGTTAAGGAGCT2480.11027417361067882No Hit
AAGTCAGGGTACCACAGAAATGTGTTCTGAGTTTTTCTTACCAATCTAAGCC2420.10760625005558173No Hit
GCAGTTTGGGCTGGGCTCGGCTGGGCGGTTCTTCTGTGAGTTGCTGCTTGGG2400.10671694220388273No Hit
GCTGGCGGGAGTGATATTTTAATTCAATTAGTGTCTTACCAATTTGATGCAG2350.10449367257463517No Hit
GCTAGAGGGCATTTGAATGGAACTGAATAATTTTCAAAGGAAGTGGTCATAC2330.10360436472293615No Hit
CATGCCTGTGGTCCCAGCACTTTGGAAGACCGAGGTGGGTGGATCACTTGGA2300.10227040294538761No Hit
GTCCCTGGGCCTTTCATTTCCCCCATGCTGATTGAGTGTTTTCCTGTACCAT2290.10182574901953809No Hit
GTTCCGGGGGATCTACGCGCGGGACTAAAATGCAGATTCTGATTCAGTAAAT2260.10049178724198955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)