FastQCFastQC Report
Sat 31 Dec 2022
SRR5848586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226650
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC6800.300022060445621No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT6460.28502095742333994Illumina PCR Primer Index 12 (95% over 21bp)
AGCAAAGGGAAATTAACCGGCCTTCCAACTTTTGTCTGCCTCATTCTAAAAT2990.13192146481358924No Hit
GATACCGGGGATCTAACGCGGGATAATCAAAATTCCTAGAAGAGGAACAACA2720.1200088241782484No Hit
CTCAGTGGGACTCTAATGTAAACTGTGGACTTTAGTTAATACTAATGTGTCA2690.11868519744098831No Hit
AAGTCAGGGTACCACAGAAATGTGTTCTGAGTTTTTCTTACCAATCTAAGCC2650.11692036179130817No Hit
ACTCGGGAGGATAAGCTCTGCAGTACTGCAGATTAAAGAAAATGGAATGTCA2560.1129494815795279No Hit
AGATGTGGGCTGTTTCAATCCTTTCCTAGAGCAACCCTGTTTTAGGGGAACA2540.11206706375468783No Hit
AGCATCGGGCAACATGTGGCTTATATAATTCCCACACATAAAGTTCCAATAG2500.11030222810500773No Hit
TGGATTGGGACCCAGAGAAGCTGATGAGCTCTGCCAGCCTTGTTAAGGAGCT2490.1098610191925877No Hit
GCCAGAGGGGGGGATCGCGGGCGCCGGCTGAGCCAGCGGCTCTTGGGAGGCT2460.10853739245532759No Hit
TGTCGACGGGATGGATCTGTGATGGCACTGGGATTGCCAATGAGTGAAATAG2460.10853739245532759No Hit
AGCCGGGGGGGCATGCTCGAAGGGTGGGGGTTGAGGCCGACGGGGCGCCGTA2370.10456651224354731No Hit
ACCAGGGGGAACTGCTATGACGGTTAAGAATGTCAGTATACAAGAAGGAATA2360.10412530333112728No Hit
TTATGCGGTGGACAGGAATCCTATTTTAGACAGAGGACTGAAAGTAGTAAAT2320.10236046768144716No Hit
GCAGTTTGGGCTGGGCTCGGCTGGGCGGTTCTTCTGTGAGTTGCTGCTTGGG2300.1014780498566071No Hit
AAGCTTGGGCAACAGAGCAAGACCCTGTCTCCGAAACAAATAAAAAATACTG2270.100154423119347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)