FastQCFastQC Report
Sat 31 Dec 2022
SRR5848582.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848582.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91798
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGC10401.1329222858885815No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCTCGTATGCCGTCT8140.8867295583781782Illumina PCR Primer Index 12 (95% over 21bp)
CTGCACGGGATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGT2060.2244057604740844No Hit
TTTAAAAGGGAGGATTCGAGAAGATATTGCAAAATATTTGAGGAATTTAGAT1730.18845726486415826No Hit
TCAATGGGGATTGGAAAGCCAGAAGTTGAATATGATTGTGATAATTTGCAAC1720.18736791651234233No Hit
TGTGGCGGCATTAAGTATAAAATAATTAAACATTCAAATCAAAAGGGAGAAA1700.18518921980871045No Hit
CAAAGGGGGCAAAGAACAAGTCATTAGAGAAATTAAAAATTATTTTGAACTA1680.18301052310507854No Hit
GGTATGGGGGGGCGGGGGCCGGCAGGTGCTCCGCAGCCGTCTGTGCCACCCA1610.17538508464236693No Hit
ACTGCAGGGATGCGTACCAGGGGATGGATTTAAGCATTTGTGTGTAATAGGA1540.16775964617965533No Hit
TAACTTGGGATGGTGGTGCAGTTCTTACGAAACTTTGGTAAAGTTTTGGGCT1530.16667029782783938No Hit
CAATGCGGGTGTGTGGCTCAGAGGTGAAGCGTTGGAAGGGTTTGTGGGAGAT1530.16667029782783938No Hit
GTTAATGGGATATTTAAACGGCTGAAGGAAAAAAACTTTTACCCAGGAATAG1500.16340225277239157No Hit
CGCACAGGGACTGTGTCTGACTCTCAACAAATAATGACAAGAAATGAAAGGC1490.1623129044205756No Hit
GAAGCGAGGATATTTAAGACTTATTGTAGGAAGTAATTTTAAATCTATTTCA1450.15795551101331184No Hit
AGTGCTGGGTATTTGATGTGTTGCCTGGCAGGATACTGCAAAGAACATGGTT1450.15795551101331184No Hit
CAACCAGGGACCTATTAGAAATGCACTTTACCTGTAAGGACACAAGTAAACT1440.1568661626614959No Hit
GGAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1420.154687465957864No Hit
TACGACAGGGTCTTCCGATCTTGCTTGGGGAGCAGAAAGAAGAAAATAACAA1410.15359811760604808No Hit
TGGTTCGGGGGAAAAAGCCTTTTAGATGAAAGTCAATCTTCGGTTTTGTTGT1400.15250876925423212No Hit
AAATGCGGGATTCCTAAGTTCTGCTTTCTGGAGAGGTCGTGAGTGGGCAGAG1380.1503300725506002No Hit
GGTAGCTGGGGTTTTTACAATGGATTCTTAGATATGACACCAAAGTACAAGC1380.1503300725506002No Hit
GTAATAGGGCACAACGTGCATTGTGCTTCTTTCTTTGGGAATATTTGAATCT1370.14924072419878429No Hit
GCTCCGGGGAAAAAGCATCTTCCATGAAACCAGTCCCTGGTGGCAAAAGGGC1350.1470620274951524No Hit
AAATATGGGAGGATCAAATCTTAATATAACTATAATCAGTTTTCTCTTTATA1350.1470620274951524No Hit
CGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1350.1470620274951524No Hit
CCTTTAGGGTGGTGAGGTCAACGTTGTCACATACCTTCACATAAGGGATAGT1340.14597267914333645No Hit
ACGGGTGGGCATGACAACTGAAATATCTTTCAAGGAAGTGATAGAAGCAATT1330.14488333079152052No Hit
TGTTGCGGGATGTGAAAAATGTAATTTACTAAAACTGACACATGAAGAAATA1320.14379398243970457No Hit
AATGTGGGCAGCCACGACTCCTCCTTCCTCTGCGCTTCCAGTGATAAGGGTA1310.14270463408788864No Hit
ATACCGGGGAGCTGATTAGTAGGTGAGATCATGAAAGAGTTGACTGCAGAGG1290.14052593738425675No Hit
GCGCATGGGACTGTTGGTGGGAAAGTGAAATGGTATAGCCACTGTGGACAAT1270.13834724068062487No Hit
GCTTTACGGGGTAGTGAAACATCAAGTCCAATTGCAGGCAACTCTCCCTTTT1260.13725789232880892No Hit
CGCAATGGGGATCCAAAGAGCCCTTCGAGCACCACGCAAGAAGATCCATCGC1260.13725789232880892No Hit
TATGTAGGGAGCTATGACTATCTCCAAAATGCACCTCCTGGATTTTTTCCGA1260.13725789232880892No Hit
CTCTGAGGGATTCCCGGGCTCGATGCCGGAAAAACACCTTGGCCCGCCGGAA1230.13398984727336108No Hit
AACTAGGGATATAAGACATTATTGAAGCCTATCACAAAATCTAAGAAGTTTG1230.13398984727336108No Hit
GAATTAGGGTGAGTGTCTTAATATTTCTCAAAATTAATTTTTATATATTTAT1210.1318111505697292No Hit
TATCAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTT1200.13072180221791324No Hit
AACTTAGGGAGAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTTTCCCG1200.13072180221791324No Hit
AACGCAGGGATCCCACCTACAAGTATGACACCTGTGTAACACCAGCTAGGTA1200.13072180221791324No Hit
AACTATGGGGGCTAATGAGTCATTGTTCCTTAATATCGTTTTCAATATGCCA1180.12854310551428136No Hit
ACCATGGGGGATCTCATAGAGGGAAGGATGTTGTGGAACAAATGGAAAAATG1170.1274537571624654No Hit
CTCAAAGGGAGGATGAGTTCAACAATGACTTAATAGCATCAGAATTTTGACA1160.12636440881064948No Hit
ATCTGAGGGATTGGCTGTCATGAATATTACTGGTCTTCCGGACTTCCAAAGG1150.12527506045883352No Hit
AACATGGAGGACAAAGTAGGTGTGTCACGGAAGAAAGTGCTTAGGGAAGATT1150.12527506045883352No Hit
TTGACTCGGGCGGTCGAGTCACCCCCAACCTCATGGGCCACCTCTGTGGAAA1140.12418571210701759No Hit
CATACGGGGAGAGATCACAGTGGAGCACTTTGAAAGACCGTCGGTTGTGTGC1130.12309636375520165No Hit
TTACTAGGGATCTGTGATTGATAAATATTTGTTTCATGAAGGAACCTGTGAG1130.12309636375520165No Hit
CTGCTGGGCGGCCCAGAAGGTTATATTTAAAAAAGAAAGTTTCTCTTTTGAA1120.12200701540338571No Hit
TATAGGGGACACATCAACAGGAAGGTAAGACAGCATTGCTGGGCCAAAAGTC1120.12200701540338571No Hit
GCGTTCGGGTGTTTGCAGACAACATGATTCTATATTTAGAAAACACCATCAT1120.12200701540338571No Hit
ATAGAAGGGAAGGAAGGAGTAGGTCGGGAAGTTCTAGTAGTGGTAGTTCTAG1110.12091766705156975No Hit
CAGTATGGGGAAAGGAAAGAGGTCGGGAGCGCTCGCGAGATCTCGGACCACC1110.12091766705156975No Hit
GGTGCCGGGGGATACTTCTAATAAATGGAATTTCATTTTATAGACTGTATAT1100.1198283186997538No Hit
TAACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1100.1198283186997538No Hit
GATCAAGGGATCAATGATTAACGGAAGTCTTGCAAAAGAAAGAAGGGAAATC1100.1198283186997538No Hit
GTTTAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTT1090.11873897034793786No Hit
TGTAATGGGATTAGAAAGGCTCAATAGTAGATTTGAACTGGGAGAAGAAACA1080.11764962199612192No Hit
CGTCACGGGTCTACTCAACAACTGCAGGATATACATTACTTTCTTCAACACA1070.11656027364430598No Hit
AGGCCGGGTGCCCTAACCCTCAAAGGAAAGGAGTCATCTTCTAGAAAGCAAC1070.11656027364430598No Hit
GGTCACGGGACCATATGTTTCTTTTTTGTGTAGATATTAATAACATTACTCT1060.11547092529249003No Hit
CTCTCAGGGACATACTCATAAGACAGAATTAAATCAGACAATACAAGAACTG1060.11547092529249003No Hit
TCTTTAGGGACTGGCCTCCCTTGCAGGCCACCTCTCTTTCTTTCTTGGAAGA1060.11547092529249003No Hit
CTCGTGGGATGGTACATTTCTGTAACTTCTTTTTAATTTTGCTGTGAACCTA1060.11547092529249003No Hit
GCAAAAGGGCTCCCAGGTGTTGTCCAAGACCATCAAGGTAGTACGTCTACGA1050.11438157694067408No Hit
TTTGTGCGGGACTTCTTCTCGGTCTTCTTCTCCTAAAAGGAAAAAGAGACAC1050.11438157694067408No Hit
AAACTTGGGGCTGTCCAATAGAACTTTCTGTGATGATGAAAACGTGTAAAGT1050.11438157694067408No Hit
TCTAGTGGGGTCAGGTCCACGGGCCTGCCTACATTTTCTTGAAAATACCATG1040.11329222858885814No Hit
GGTATTGGGGAGGTTGTATGTTTTGTCTAGAAAATATATAAACTACAAAAAG1030.1122028802370422No Hit
TGGCTTGGGATTGATCATCTTGACCAATATGCTTGAGAAAGGACAGTTAGAT1020.11111353188522625No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTTTGTC1010.11002418353341031No Hit
GTGTAAGGGATAGTCCCGGCATGGGGACCTCAACACACCTGAGCCCCAGACA1000.10893483518159437No Hit
TCTAACGGGATGAACTCTAGGGCAATTACTATTTCTAAAAATGTATGGTGGA990.10784548682977843No Hit
AATCTGGGACTATTTATTGAGTGCCTATTATGTGCCTGGCTCTGTGCTAGGC990.10784548682977843No Hit
TTACGTGGGAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAAT990.10784548682977843No Hit
GAGCAGGGGCAGAGCAAGATACTTAATCACATCTGCATAGACTCCTTTTCCT980.10675613847796249No Hit
TCAACAGGGAGAGGTGGCAATCCTAATTTCAGACAAAACACACTTTGAACCA980.10675613847796249No Hit
TCTAATGGGAAAAGAAAAATATTTTTTAAACCGAAATCTGTGGATGAAATAG970.10566679012614655No Hit
GTTCCTGGCACACTGGGCAGAATGTATCATGAGGCCACACCTAGCTGCAAAG970.10566679012614655No Hit
AATCTTGGGATGTGCCCTGTGCGCCAGAGGACGGTATCTTCTATGTCCGCCT960.1045774417743306No Hit
AGAAATGGGCTACCACACCTGACTTGTTCCTTATTTTAGATGTTGAAAATAA960.1045774417743306No Hit
CATGCGGGGAATGCTATCACTGCCAAGAGAAATTAAAATCTAAACAAACGTA960.1045774417743306No Hit
CGCCATGGGGGTTCTGGGATACATGTGCAGAACGTGCAGGTTTGTTGCATAG960.1045774417743306No Hit
AAGCACGGGCATAGAGTGGTCTTTTGAACTGGTATCTAAACATACCTGGTTA950.10348809342251464No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCACTGTCTCTTATACAC950.10348809342251464No Hit
GCTTTGTGGGAAGACCATGTTGTAGAAGAATCTTTAATGTTGTTGGAAAAAT950.10348809342251464No Hit
CGTCCCGGGATTGCTGATAGGATTATATTTAATGTGAATAATTTTTAAAATT950.10348809342251464No Hit
AAGCCAGGGAGTGGGAACAGGCCCCAGCTCAGCCTCCGGCAGGGAGGTCACC950.10348809342251464No Hit
ATCAACGGGATGGTCACCAGCCCAAGGCTGCCTCATGCTCTTCGTGACTTAC940.1023987450706987No Hit
TTATGAGGGCGAGCATGTTTTGAAGAGGTAGTTAGTCAATCTTTATGTCCCT940.1023987450706987No Hit
GCGCACGGATAGGCTTTCCCAACAGCTGCCTCTGAAATAGCTGCTGCTTCGA940.1023987450706987No Hit
ACAGCAGGGATCACTGACACTCCAGACTAAAGCAGAGTCTAGGTTTCTGCAA940.1023987450706987No Hit
CACATAGGGATTTCAGGGATGGAACACGGACCAGCTGACCTTAGCGTGGTCG940.1023987450706987No Hit
TCTATAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT930.10130939671888275No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTTTGTCATCCTGTCTCTTATAC930.10130939671888275No Hit
TGCCGGGGCAGGTTTATACAGAGATGTCTAGACTGCTTCCTCCTGATTAGAA920.10022004836706681No Hit
GTCAGAGGGCGATCTCAGTAAGGGCGATCTAAGCTTGGGCTTGGCCAGGAAC920.10022004836706681No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)